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公开(公告)号:US20180195950A1
公开(公告)日:2018-07-12
申请号:US15848450
申请日:2017-12-20
发明人: James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC分类号: G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26
摘要: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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公开(公告)号:US10955332B2
公开(公告)日:2021-03-23
申请号:US15848450
申请日:2017-12-20
发明人: James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC分类号: G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26 , B01J19/00 , G01N15/10
摘要: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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公开(公告)号:US20210208055A1
公开(公告)日:2021-07-08
申请号:US17191612
申请日:2021-03-03
发明人: James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC分类号: G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26 , B01J19/00
摘要: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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公开(公告)号:US20180327832A1
公开(公告)日:2018-11-15
申请号:US15969562
申请日:2018-05-02
发明人: Sean M. Ramirez , Brian D. Mather , Edwin Li , Sojeong Moon , Innsu Daniel Kim , Alexandre Richez , Ludovic Vincent , Xavier von Hatten , Hai Quang Tran , Maxwell Zimmerley , Julia Morrison , Gianluca Andrea Artioli , Krystal Sly , Hayden Black , Lewis J. Kraft , Hong Xie , Wei Wei , Ryan Sanford
IPC分类号: C12Q1/6874 , C12Q1/6806
CPC分类号: C12Q1/6874 , C12Q1/6806
摘要: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.
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公开(公告)号:US20190151850A1
公开(公告)日:2019-05-23
申请号:US15572128
申请日:2016-05-04
申请人: Illumina, Inc.
发明人: Allen E Eckhardt , Rijo Pantoja , Sean M Ramirez , Petr Capek , Edwin Li
IPC分类号: B01L3/00
CPC分类号: B01L3/502784 , B01L3/502707 , B01L2200/0673 , B01L2200/141 , B01L2300/0645 , B01L2300/0816 , B01L2300/0887 , B01L2300/16 , B01L2300/161 , B01L2300/163 , B01L2400/0427
摘要: Embodiments of present application are directed to microfluidic devices and particularly digital micro-plastic fluidic devices that are specifically designed to prevent sample contamination during sample processing, methods of manufacturing the same, and methods to improve sample analysis process by preventing sample contamination.
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公开(公告)号:US10589273B2
公开(公告)日:2020-03-17
申请号:US15572128
申请日:2016-05-04
申请人: Illumina, Inc.
发明人: Allen E Eckhardt , Rigo Pantoja , Sean M Ramirez , Petr Capek , Edwin Li
摘要: Embodiments of present application are directed to microfluidic devices and particularly digital micro-plastic fluidic devices that are specifically designed to prevent sample contamination during sample processing, methods of manufacturing the same, and methods to improve sample analysis process by preventing sample contamination.
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