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公开(公告)号：US20210208055A1
公开(公告)日：2021-07-08
申请号：US17191612
申请日：2021-03-03
发明人： James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC分类号： G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26 , B01J19/00
摘要： A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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公开(公告)号：US20200179935A1
公开(公告)日：2020-06-11
申请号：US16790147
申请日：2020-02-13
发明人： Xavier von Hatten , Michel Perbost , Heng Huang , Nicole Lee , Vicky V. Lam , Nilda Juan , Timothy J. Merkel , John M. Beierle
摘要： Disclosed herein are compositions and fluidic devices that include a filler fluid having a siloxane block co-polymer solubilized in the filler fluid. Also disclosed herein are related kits and methods for using the fluidic devices for various uses, such as the polymerase chain reaction or preparations for sequencing reactions.
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公开(公告)号：US20180195950A1
公开(公告)日：2018-07-12
申请号：US15848450
申请日：2017-12-20
发明人： James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC分类号： G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26
摘要： A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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4.发明授权公开(公告)号：US12084474B2
公开(公告)日：2024-09-10
申请号：US16411913
申请日：2019-05-14
发明人： Xiaolin Wu , Randall Smith , Peyton Shieh , John M. Beierle , Wayne N. George , Elliot John Lawrence , Jie Mao , Xiaohai Liu
IPC分类号： C07H21/04 , C12Q1/6806 , C12Q1/6876
CPC分类号： C07H21/04 , C12Q1/6806 , C12Q1/6876 , C12Q1/6806 , C12Q2523/107 , C12Q2525/113 , C12Q2565/543
摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.
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公开(公告)号：US10955332B2
公开(公告)日：2021-03-23
申请号：US15848450
申请日：2017-12-20
发明人： James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC分类号： G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26 , B01J19/00 , G01N15/10
摘要： A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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公开(公告)号：US20190217300A1
公开(公告)日：2019-07-18
申请号：US15770017
申请日：2016-10-20
发明人： Xavier von Hatten , Michel Perbost , Heng Huang , Nicole Lee , Vicky V. Lam , Nilda Juan , Timothy J. Merkel , John M. Beierle
CPC分类号： B01L3/502792 , B01J19/0093 , B01J2219/00813 , B01L2300/12 , B01L2400/0418 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , C08G77/04 , C08G77/388 , C08G77/392 , C08G77/44 , C08L83/10 , C08L83/00
摘要： Disclosed herein are compositions and fluidic devices that include a filler fluid having a siloxane block co-polymer solubilized in the filler fluid. Also disclosed herein are related kits and methods for using the fluidic devices for various uses, such as the polymerase chain reaction or preparations for sequencing reactions.
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公开(公告)号：US11846580B2
公开(公告)日：2023-12-19
申请号：US17191612
申请日：2021-03-03
发明人： James Tsay , Anmiv Prabhu , David Heiner , Edwin Li , Alexandre Richez , John M. Beierle , Kevan Samiee , Kristina Munoz , Leonid Malevanchik , Ludovic Vincent , Naiqian Zhan , Peyton Shieh , Robert Yang , Samantha Schmitt , Sang Park , Scott Bailey , Sean M. Ramirez , Sunmin Ahn , Valerie Uzzell , Wei Wei , Yuxiang Huang , Tyler Jamison Dill
IPC分类号： G01N15/14 , C12Q1/6874 , B01L3/00 , C09D133/24 , C09D133/26 , G01N15/10 , B01J19/00
CPC分类号： G01N15/1436 , B01J19/0046 , B01L3/502 , B01L3/502707 , C09D133/24 , C09D133/26 , C12Q1/6874 , G01N15/1484 , B01J2219/00286 , B01J2219/00317 , B01J2219/00319 , B01J2219/00418 , B01J2219/00585 , B01J2219/00596 , B01J2219/00612 , B01J2219/00637 , B01J2219/00722 , B01L2200/12 , B01L2300/087 , B01L2300/0829 , B01L2300/0848 , B01L2300/0887 , B01L2300/16 , G01N2015/1006
摘要： A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.
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8.发明申请公开(公告)号：US20190352327A1
公开(公告)日：2019-11-21
申请号：US16411913
申请日：2019-05-14
发明人： Xiaolin Wu , Randall Smith , Peyton Shieh , John M. Beierle , Wayne N. George , Elliot John Lawrence , Jie Mao , Xiaohai Liu
IPC分类号： C07H21/04 , C12Q1/6876
摘要： Embodiments of the present disclosure relate to methods of preparation of templates for polynucleotide sequencing. In particular, the disclosure relates to linearization of clustered polynucleotides in preparation for sequencing by cleavage of one or more first strands of double-stranded polynucleotides immobilized on a solid support by a transition metal complex, for example, a palladium complex or a nickel complex. Further disclosure relate to linearization of clustered polynucleotides by cleaving one or more second strands of double double-stranded polynucleotides immobilized on a solid support comprising azobenzene linker by Na2S2O4. Nucleotides and oligonucleotides comprising a 3′ phosphate moiety blocking group, and methods of removing the same using a fluoride reagent are also disclosed.
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公开(公告)号：US11692223B2
公开(公告)日：2023-07-04
申请号：US16846028
申请日：2020-04-10
发明人： Kevin L. Gunderson , Jingwei Bai , Matthew William Kellinger , John M. Beierle , Jonathan Mark Boutell , Roberto Rigatti , Maria Candelaria Rogert Bacigalupo , Boyan Boyanov , Klaus Maisinger
IPC分类号： C12P19/34 , C12Q1/6874 , B01J19/00 , C12Q1/6806 , C12Q1/6837 , C12Q1/6853 , C40B40/06 , C40B50/14
CPC分类号： C12Q1/6874 , B01J19/0046 , C12Q1/6806 , C12Q1/6837 , C12Q1/6853 , C40B40/06 , C40B50/14 , B01J2219/00317 , B01J2219/00585 , B01J2219/00596 , B01J2219/00608 , B01J2219/00637 , B01J2219/00722 , C12Q1/6874 , C12Q2565/513 , C12Q2565/543
摘要： The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods, including methods for modifying immobilized capture primers comprising: a) contacting a substrate comprising a plurality of immobilized capture primers with a plurality of template nucleic acids under conditions sufficient for hybridization to produce one or more immobilized template nucleic acids, and b) extending one or more immobilized capture primers to produce one or more immobilized extension products complementary to the one or more template nucleic acid.
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公开(公告)号：US20200318182A1
公开(公告)日：2020-10-08
申请号：US16846028
申请日：2020-04-10
发明人： Kevin L. Gunderson , Jingwei Bai , Matthew William Kellinger , John M. Beierle , Jonathan Mark Boutell , Roberto Rigatti , Maria Candelaria Rogert Bacigalupo , Boyan Boyanov , Klaus Maisinger
IPC分类号： C12Q1/6874 , B01J19/00 , C12Q1/6806 , C12Q1/6837 , C12Q1/6853 , C40B40/06 , C40B50/14
摘要： The present disclosure relates to the field of molecular biology and more specifically to microarrays and methods.
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