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公开(公告)号：US20130004988A1
公开(公告)日：2013-01-03
申请号：US13608081
申请日：2012-09-10
Applicant: Michael J. Pugia , James A. Profitt , Lloyd S. Schulman , Chris T. Zimmerle , Hai-Hang Kuo
Inventor： Michael J. Pugia , James A. Profitt , Lloyd S. Schulman , Chris T. Zimmerle , Hai-Hang Kuo
CPC classification number: B01L3/0268 , B01J2219/0036 , B01J2219/00378 , B01J2219/00385 , B01J2219/00527 , B01J2219/00545 , B01J2219/00641 , B01J2219/00725 , B01J2219/0074 , B01L2400/0487 , G01N33/52 , G01N35/1016 , G01N2035/00158 , Y10T436/144444 , Y10T436/2575
Abstract: Biological fluid samples are deposited by methods that produce a uniform layer of the sample over a reagent-containing surface. In one embodiment, a nozzle having multiple openings is used to deposit a sample over the reagent-containing surface simultaneously. In an alternative embodiment, single droplets of the sample are deposited in a pattern on the surface, preferably in a sequence of parallel lines. The reaction between the biological sample and the reagents is read from a spectrographic image of the reagent-containing surface obtained by optical methods.
Abstract translation: 通过在含试剂的表面上产生均匀的样品层的方法沉积生物流体样品。 在一个实施例中，使用具有多个开口的喷嘴同时将试样沉积在含有试剂的表面上。 在替代实施例中，样品的单个液滴以图案沉积在表面上，优选地以一系列平行线沉积。 从通过光学方法获得的含有试剂的表面的光谱图像中读取生物样品和试剂之间的反应。
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2.发明授权Method and apparatus for splitting of specimens into multiple channels of a microfluidic device 有权将样品分离成微流体装置的多个通道的方法和装置公开(公告)号：US07125711B2
公开(公告)日：2006-10-24
申请号：US10326157
申请日：2002-12-19
Applicant: Michael J. Pugia , James A. Profitt , Gert Blankenstein , Ralf-Peter Peters
Inventor： Michael J. Pugia , James A. Profitt , Gert Blankenstein , Ralf-Peter Peters
CPC classification number: B01L3/5027 , B01L3/5025 , B01L3/50273 , B01L2200/0605 , B01L2200/0684 , B01L2300/0803 , B01L2300/0816 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2400/0406 , B01L2400/0409 , B01L2400/0688 , G01N35/00069 , G01N2035/00158 , G01N2035/00237 , G01N2035/1034
Abstract: A microliter liquid sample, particularly a biological sample, is analyzed in a device employing centrifugal and capillary forces. The sample is moved by capillary forces into one or more metering wells which define the amount of the sample to be analyzed in subsequent steps. The defined amount of the sample is transferred from the metering wells to one or more conditioning and reagent wells for measuring the amount of an analyte contained in each metered amount of the sample.
Abstract translation: 在使用离心和毛细管力的装置中分析微升液体样品，特别是生物样品。 样品通过毛细管力移动到一个或多个计量孔中，其限定在后续步骤中要分析的样品的量。 将定义的样品量从计量孔转移到一个或多个调理和试剂孔，以测量每个计量量的样品中所含的分析物的量。
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公开(公告)号：US06815210B1
公开(公告)日：2004-11-09
申请号：US10480314
申请日：2003-12-11
Applicant: James A. Profitt , Alexander H. Orn , Jennifer Farr
Inventor： James A. Profitt , Alexander H. Orn , Jennifer Farr
IPC: G01N2178
CPC classification number: G01N33/6839 , G01N33/543
Abstract: An assay for the determination of protein in an aqueous test fluid which combines the test fluid with a buffer and a dye. The buffer is selected from citrulline, malonic acid, cyanoacetic acid, citraconic acid, methyl phosphonic acid, sarcosine, saccharin, or combinations thereof. The buffer is added in sufficient quantity to maintain the pH of the assay including the test fluid at a selected target pH range within a range of from about 2.0 to about 3.0. The dye has a pKa which enables it to operate as a protein indicator at the target pH range. The dye also has affinity for protein such that it will provide a detectable response in the presence of greater than about 15 mg/dL protein to thereby render the assay suitable for the detection of total protein in the test fluid. The buffer and dye may be absorbed in a test strip of absorbent material.
Abstract translation: 在测试流体与缓冲液和染料组合的水性测试流体中测定蛋白质的测定法。 缓冲液选自瓜氨酸，丙二酸，氰基乙酸，柠康酸，甲基膦酸，肌氨酸，糖精或其组合。 以足够的量加入缓冲液，以将测定液的pH值保持在约2.0至约3.0范围内的选定目标pH范围。 染料具有使其能够在目标pH范围内作为蛋白质指示剂操作的pKa。 染料还对蛋白质具有亲和力，使得其在大于约15mg / dL蛋白质的存在下提供可检测的响应，从而使测定法适合于检测测试流体中的总蛋白质。 缓冲液和染料可以吸收在吸收材料的测试条中。
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公开(公告)号：US4943571A
公开(公告)日：1990-07-24
申请号：US335354
申请日：1989-04-10
Applicant: Helen H. Ong , Vernon B. Anderson , James A. Profitt
Inventor： Helen H. Ong , Vernon B. Anderson , James A. Profitt
IPC: C07D313/14
CPC classification number: C07D313/14
Abstract: Novel aminoalkylthiodibenzoxepins, physiologically tolerable acid addition salts thereof, a method of preparing same, pharmaceutical and veterinary preparations including same and methods of treating by administering same are disclosed. These compounds are useful as analgesic, antidepressant and anticonvulsant agents. A process for selectively reducing olefins by alkaline earth metals in loweralkanols is also disclosed.
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公开(公告)号：US4775683A
公开(公告)日：1988-10-04
申请号：US14748
申请日：1987-02-13
Applicant: Helen H. Ong , Vernon B. Anderson , James A. Profitt
Inventor： Helen H. Ong , Vernon B. Anderson , James A. Profitt
IPC: C07D337/14 , A61K31/38
CPC classification number: C07D337/14
Abstract: Novel aminoalkylthiodibenzothiepins and related compounds, physiologically tolerable acid addition salts thereof, a method of preparing same, pharmaceutical and veterinary preparations including same and methods of treating by administering same are disclosed. These compounds are useful as antidepressant, analgetic, and anticonvulsant agents.
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Patent Agency Ranking6.发明授权
公开(公告)号：US4524207A
公开(公告)日：1985-06-18
申请号：US515969
申请日：1983-07-21
Applicant: Helen H. Ong , Vernon B. Anderson , James A. Profitt
Inventor： Helen H. Ong , Vernon B. Anderson , James A. Profitt
IPC: C07D211/52 , C07D211/54 , C07D495/10 , C07D513/10
CPC classification number: C07D495/10 , C07D211/52 , C07D211/54
Abstract: Novel substituted 1,3-dihydrospiro[benzo(c)thiophene]s and methods of preparing the same are described. These compounds are useful as antidepressants and tranquilizers and intermediates therefor.
Abstract translation: 描述了新型取代的1,3-二氢螺[苯并（c）噻吩]及其制备方法。 这些化合物可用作抗抑郁药和镇定剂及其中间体。
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公开(公告)号：US4477667A
公开(公告)日：1984-10-16
申请号：US332251
申请日：1981-12-18
Applicant: Helen H. Ong , James A. Profitt
Inventor： Helen H. Ong , James A. Profitt
IPC: C07D211/64 , C07D471/10
CPC classification number: C07D471/10 , C07D211/64
Abstract: Novel spiro[indoline-3,4'-piperidine]s and related compounds and methods of preparing same are described. These compounds are useful as antidepressants, anticonvulsants and tranquilizers. Also described is a novel method of preparing indoline rings.
Abstract translation: 描述了新的螺[二氢吲哚-3,4'-哌啶]和相关化合物及其制备方法。 这些化合物可用作抗抑郁药，抗惊厥药和镇定剂。 还描述了制备二氢吲哚环的新方法。
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公开(公告)号：US08304254B2
公开(公告)日：2012-11-06
申请号：US12598150
申请日：2008-03-14
Applicant: Michael J. Pugia , James A. Profitt
Inventor： Michael J. Pugia , James A. Profitt
IPC: G01N1/38
CPC classification number: G01N35/1016 , G01N2035/1034 , Y10T436/144444 , Y10T436/25 , Y10T436/2575
Abstract: Assays in which samples of biological fluids are dispensed onto reagent-containing porous substrates are improved in the accuracy and repeatability by dispensing the biological fluids in two or more fractions thereof, separated by intervals in which the biological fluid is not dispensed. Reagents and other fluids may be dispensed during the intervals when the biological fluid is not dispensed. Alternatively, reagents and other fluids may be dispensed in a similar manner onto substrates already containing biological fluids.
Abstract translation: 生物流体样品分配到含有试剂的多孔基材上的测定通过将生物流体分配在两个或更多个部分中，通过分配生物流体不分配的间隔而提高了精度和重复性。 在不分配生物流体的间隔期间，可以分配试剂和其它流体。 或者，可以以类似的方式将试剂和其它流体分配到已经含有生物流体的基底上。
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公开(公告)号：US20100255592A1
公开(公告)日：2010-10-07
申请号：US12598150
申请日：2008-03-14
Applicant: Michael J. Pugia , James A. Profitt
Inventor： Michael J. Pugia , James A. Profitt
CPC classification number: G01N35/1016 , G01N2035/1034 , Y10T436/144444 , Y10T436/25 , Y10T436/2575
Abstract: Assays in which samples of biological fluids are dispensed onto reagent-containing porous substrates are improved in the accuracy and repeatability by dispensing the biological fluids in two or more fractions thereof, separated by intervals in which the biological fluid is not dispensed. Reagents and other fluids may be dispensed during the intervals when the biological fluid is not dispensed. Alternatively, reagents and other fluids may be dispensed in a similar manner onto substrates already containing biological fluids.
Abstract translation: 生物流体样品分配到含有试剂的多孔基材上的测定通过将生物流体分配在两个或更多个部分中，通过分配生物流体不分配的间隔而提高了精度和重复性。 在不分配生物流体的间隔期间，可以分配试剂和其它流体。 或者，可以以类似的方式将试剂和其它流体分配到已经含有生物流体的基底上。
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10.发明申请公开(公告)号：US20100172801A1
公开(公告)日：2010-07-08
申请号：US12688346
申请日：2010-01-15
Applicant: Michael J. Pugia , James A. Profitt , Hai-Hang Kuo , Gert Blankenstein , Ralf-Peter Peters , Lloyd S. Schulman
Inventor： Michael J. Pugia , James A. Profitt , Hai-Hang Kuo , Gert Blankenstein , Ralf-Peter Peters , Lloyd S. Schulman
IPC: G01N30/00 , G01N33/487
CPC classification number: B01L3/502746 , B01L3/502723 , B01L2200/0684 , B01L2200/16 , B01L2300/0816 , B01L2400/0406 , B01L2400/0409 , B01L2400/0418 , B01L2400/0487 , B01L2400/0688 , B01L2400/086 , Y10T436/2575
Abstract: Analytical results obtained with microfluidic devices are improved by providing structural features in areas containing dry supported reagents, the structural features directing the flow of a sample over the area in a predetermined uniform manner and facilitating the purging of air.
Abstract translation: 通过在含有干支持试剂的区域中提供结构特征来改进用微流体装置获得的分析结果，其结构特征以预定均匀的方式引导样品流过该区域并促进空气吹扫。
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