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    MICROSCOPIC BODY ENCLOSING METHOD, MICROSCOPIC BODY DETECTION METHOD, AND MICROSCOPIC BODY DETECTION DEVICE
    1.
    发明申请
    MICROSCOPIC BODY ENCLOSING METHOD, MICROSCOPIC BODY DETECTION METHOD, AND MICROSCOPIC BODY DETECTION DEVICE 审中-公开

    公开(公告)号：US20200173925A1

    公开(公告)日：2020-06-04

    申请号：US16496197

    申请日：2018-03-28

    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

    Inventor： Hiroyuki NOJI ,  Kazuhito TABATA

    IPC: G01N21/64 ,  G01N21/31 ,  G01N1/36

    Abstract: Provided is a method of enclosing a microscopic body in at least some of a plurality of cavities formed in the surface of a substrate, including the step of arranging an insertion member above the cavity-formed surface of the substrate, determining relative positions of the insertion member and the substrate by a support section provided on the insertion member such that the bottom surface of the insertion member and the cavity-formed surface of the substrate face each other, thereby providing a solution introduction space between the bottom surface of the insertion member and the cavity-formed surface of the substrate, and providing a solution discharge space that is in communication with the solution introduction space, the solution discharge space being located above the bottom surface of the insertion member, and between the substrate and the insertion member, within the substrate and/or within the insertion member.

    METHOD FOR DETECTING TARGET MOLECULE AND KIT FOR USE IN SAID METHOD
    6.
    发明申请
    METHOD FOR DETECTING TARGET MOLECULE AND KIT FOR USE IN SAID METHOD 审中-公开

    公开(公告)号：US20170227538A1

    公开(公告)日：2017-08-10

    申请号：US15502652

    申请日：2015-09-09

    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

    Inventor： Hiroyuki NOJI

    IPC: G01N33/543

    CPC classification number: G01N33/54393 ,  C12Q1/68 ,  G01N33/543

    Abstract: Provided is a method to detect a target molecule, including a complex formation step of reacting a target molecule, a carrier modified with a first antibody which specifically binds to the target molecule, and two or more second antibodies which specifically bind to the target molecule, and are labeled with enzymes having a substrate cleaving activity and mutually different substrate specificities, to form a complex consisting of the first antibody, the target molecule and the second antibodies, on the carrier; and a detection step of reacting two or more substrates having cleavage sites by the enzymes, a fluorescent substance bound to one terminal side of each of the cleavage sites and a quencher bound to another terminal side thereof, wherein the fluorescent substances are mutually different in fluorescence wavelength, and the complex, to detect fluorescence emitted from the fluorescent substances.

    MICROSCOPIC BODY DETECTION METHOD AND MICROSCOPIC BODY DETECTION DEVICE
    9.
    发明申请
    MICROSCOPIC BODY DETECTION METHOD AND MICROSCOPIC BODY DETECTION DEVICE 有权

    公开(公告)号：US20210278323A1

    公开(公告)日：2021-09-09

    申请号：US16496532

    申请日：2018-03-28

    Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCY

    Inventor： Hiroyuki NOJI ,  Takao ONO ,  Yoshihiro MINAGAWA

    IPC: G01N1/36 ,  G01N21/78 ,  G01N21/64 ,  G01N33/569 ,  G01N33/58 ,  B01L3/00

    Abstract: There is provided a method of detecting a microscopic body stored in a plurality of receptacles formed separately from each other. The method, which is provided as a technique for enclosing a to-be-detected substance such as nucleic acid, protein, virus, and cell by means of a simple operation in droplets of an extremely small volume and enabling highly sensitive detection, includes the steps of (1) introducing a solvent into a space between a lower layer part in which the receptacles are formed and an upper layer part facing a surface of the lower layer part in which surface the receptacles are formed, wherein the solvent contains the microscopic body; (2) introducing gas into the space to form a droplet of the solvent in the receptacles, wherein the droplet contains the microscopic body; and (3) detecting the microscopic body present in the droplet optically, electrically, and/or magnetically.

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