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    Methods
    2.
    发明授权
    Methods 有权

    公开(公告)号：US09868956B2

    公开(公告)日：2018-01-16

    申请号：US13376068

    申请日：2010-06-02

    申请人： Duy P. Nguyen ,  Heinz Neumann ,  Alexander Deiters ,  Jason Chin ,  Hrvoje Lusic

    发明人： Duy P. Nguyen ,  Heinz Neumann ,  Alexander Deiters ,  Jason Chin ,  Hrvoje Lusic

    IPC分类号： C12P21/06 ,  C12N15/67 ,  C07C233/49 ,  C07C271/22 ,  C12N9/00 ,  C12P21/02

    CPC分类号： C12N15/67 ,  C07C233/49 ,  C07C271/22 ,  C12N9/93 ,  C12P21/02

    摘要： The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.

    INCORPORATION OF METHYL LYSINE INTO POLYPEPTIDES
    3.
    发明申请
    INCORPORATION OF METHYL LYSINE INTO POLYPEPTIDES 审中-公开
    将甲基赖氨酸加入多糖中

    公开(公告)号：US20120244636A1

    公开(公告)日：2012-09-27

    申请号：US13499450

    申请日：2010-10-01

    申请人： Jason Chin ,  Duy P. Nguyen

    发明人： Jason Chin ,  Duy P. Nguyen

    IPC分类号： C12P21/00 ,  G01N33/53

    CPC分类号： C07K14/47 ,  C12N9/93 ,  C12P21/02 ,  G01N33/566

    摘要： The invention relates to A method of making a polypeptide comprising at least one Nε-methyl-lysine at a specific site in said polypeptide, said method comprising (a) genetically directing the incorporation of R—Nε-methyl-lysine into said polypeptide, wherein R comprises an auxiliary group; and (b) catalysing the removal of R from the polypeptide of (a). In particular the invention relates to such a method wherein genetically directing the incorporation of R—Nε-methyl-lysine into said polypeptide comprises arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, and wherein said translation is carried out in the presence of an amber tRNA charged with R—Nε-methyl-lysine.

    摘要翻译： 本发明涉及一种在所述多肽的特定位点制备包含至少一个N - 甲基 - 赖氨酸的多肽的方法，所述方法包括（a）将R-N' - 甲基 - 赖氨酸掺入所述多肽 其中R包括辅助基团; 和（b）催化从（a）的多肽中除去R。 特别地，本发明涉及这样一种方法，其中将R-N'-甲基 - 赖氨酸并入所述多肽的方法包括安排编码所述多肽的RNA的翻译，其中所述RNA包含琥珀密码子，并且其中所述翻译 在装有R-N'-甲基 - 赖氨酸的琥珀色tRNA存在下进行。

    Methods
    4.
    发明申请
    Methods 有权
    方法

    公开(公告)号：US20120077948A1

    公开(公告)日：2012-03-29

    申请号：US13376068

    申请日：2010-06-02

    申请人： Duy P. Nguyen ,  Heinz Neumann ,  Alexander Deiters ,  Jason Chin ,  Hrvoje Lusic

    发明人： Duy P. Nguyen ,  Heinz Neumann ,  Alexander Deiters ,  Jason Chin ,  Hrvoje Lusic

    IPC分类号： C12P21/00 ,  C07C233/49 ,  C07C271/22 ,  C08H1/00 ,  C07K14/00

    CPC分类号： C12N15/67 ,  C07C233/49 ,  C07C271/22 ,  C12N9/93 ,  C12P21/02

    摘要： The invention relates to a method of making a polypeptide comprising an orthogonal functional group, said orthogonal functional group being comprised by an aliphatic amino acid or amino acid derivative, said method comprising providing a host cell; providing a nucleic acid encoding the polypeptide of interest; providing a tRNA-tRNA synthetase pair orthogonal to said host cell; adding an amino acid or amino acid derivative comprising the orthogonal functional group of interest, wherein said amino acid or amino acid derivative is a substrate for said orthogonal tRNA synthetase, wherein said amino acid or amino acid derivative has an aliphatic carbon backbone; and incubating to allow incorporation of said amino acid or amino acid derivative into the polypeptide of interest via the orthogonal tRNA-tRNA synthetase pair. The invention also relates to certain amino acids, and to polypeptides comprising same.

    摘要翻译： 本发明涉及一种制备包含正交官能团的多肽的方法，所述正交官能团由脂肪族氨基酸或氨基酸衍生物构成，所述方法包括提供宿主细胞; 提供编码目的多肽的核酸; 提供与所述宿主细胞正交的tRNA-tRNA合成酶对; 加入包含所述正交官能团的氨基酸或氨基酸衍生物，其中所述氨基酸或氨基酸衍生物是所述正交tRNA合成酶的底物，其中所述氨基酸或氨基酸衍生物具有脂族碳骨架; 并孵育以允许所述氨基酸或氨基酸衍生物通过正交的tRNA-tRNA合成酶对引入目的多肽。 本发明还涉及某些氨基酸，以及包含其的多肽。

    Twin buckle assembly with dual release positions
    5.
    发明授权
    Twin buckle assembly with dual release positions 有权
    带双释放位置的双扣组件

    公开(公告)号：US08096027B2

    公开(公告)日：2012-01-17

    申请号：US12546914

    申请日：2009-08-25

    申请人： Marc Jung ,  Jason Chin

    发明人： Marc Jung ,  Jason Chin

    IPC分类号： A44B11/25

    CPC分类号： A44B11/2542 ,  A44B11/2526 ,  Y10T24/45613 ,  Y10T24/45644 ,  Y10T24/45649

    摘要： A twin buckle assembly is provided with dual release positions. The buckle assembly can lockingly secure two buckle tongues. The buckle assembly includes a single, pivotal latch mechanism that can separately secure one or both of the buckle tongues. Pivotal movement of the latch occurs upon rotation of the buckle cover. Thus, one tongue is released at a first rotational position of the cover and both tongues are released at a second rotational position of the cover. The buckle assembly, thereby, allows the independent removal of lap and shoulder belts.

    摘要翻译： 双扣组件提供双释放位置。 带扣组件可以锁定两个扣舌。 带扣组件包括单独的枢转闩锁机构，其可以单独地固定一个或两个带扣舌。 闩锁的枢转运动发生在带扣盖旋转时。 因此，一个舌片在盖的第一旋转位置被释放，并且两个舌片在盖子的第二旋转位置被释放。 因此，带扣组件可以独立地拆卸搭扣和肩带。

    Methods and Compositions
    6.
    发明申请
    Methods and Compositions 审中-公开
    方法和组成

    公开(公告)号：US20110027829A1

    公开(公告)日：2011-02-03

    申请号：US12739247

    申请日：2008-10-27

    申请人： Heinz Neumann ,  Jason Chin

    发明人： Heinz Neumann ,  Jason Chin

    IPC分类号： C12P21/00 ,  C12N9/00 ,  C07H21/00 ,  C07K2/00 ,  C07K14/00 ,  C12N15/63 ,  C12N1/00

    CPC分类号： C12P21/02 ,  C12N9/93

    摘要： The invention relates to a tRNA synthetase capable of binding N⊂-acetyl lysine, in particular, the invention relates to a tRNA synthetase capable of binding N∈-acetyl lysine wherein said synthetase comprises a polypeptide having at least 90% sequence identity to the amino acid sequence of MbPy1RS. The invention also relates to a method of making a polypeptide comprising N∈-acetyl lysine comprising arranging for the translation of a RNA encoding said polypeptide, wherein said RNA comprises an amber codon, wherein said translation is carried out in the presence of a polypeptide according to any of claims 1 to 11 and in the presence of tRNA capable of being charged with N∈-acetyl lysine, and in the presence of N∈-acetyl lysine.

    摘要翻译： 本发明涉及能够结合N-乙酰赖氨酸的tRNA合成酶，特别地，本发明涉及能够结合Nε-乙酰赖氨酸的tRNA合成酶，其中所述合成酶包含与氨基具有至少90％序列同一性的多肽 MbPy1RS的酸序列。 本发明还涉及一种制备包含Nε-乙酰赖氨酸的多肽的方法，包括安排编码所述多肽的RNA的翻译，其中所述RNA包含琥珀密码子，其中所述翻译在多肽存在下进行， 权利要求1至11中任一项，并且在存在能够被Nε-乙酰赖氨酸加载的tRNA存在下，并且在Nε-乙酰赖氨酸存在下。

    Evolved Orthogonal Ribosomes
    7.
    发明申请
    Evolved Orthogonal Ribosomes 失效
    进化正交核糖体

    公开(公告)号：US20100105565A1

    公开(公告)日：2010-04-29

    申请号：US12516230

    申请日：2007-11-28

    申请人： Jason Chin ,  Kaihang Wang ,  Heinz Neumann

    发明人： Jason Chin ,  Kaihang Wang ,  Heinz Neumann

    IPC分类号： C40B30/00 ,  C07H21/02 ,  C12P21/06 ,  C12N1/21

    CPC分类号： C12N15/11 ,  C12N15/111 ,  C12N2320/13 ,  C12P21/02 ,  C40B10/00 ,  C40B40/08

    摘要： There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.

    摘要翻译： 提供了用于进化正交rRNA分子的方法，包括以下步骤：提供一个或多个突变正交rRNA分子文库，并将文库引入细胞，使得正交rRNA被并入核糖体中以提供正交核糖体; 提供（i）不被天然核糖体翻译的一个或多个正交mRNA分子，和（ii）包含一个或多个正交mRNA密码子; 测定正交mRNA的翻译并选择翻译正交mRNA的正交rRNA分子，其中步骤（c）中的测定需要在正交mRNA中翻译一个或多个正交mRNA密码子; 并结合这样的rRNA分子的正交核糖体。

    Traceless Ubiquitination
    8.
    发明申请
    Traceless Ubiquitination 审中-公开
    无痕泛素化

    公开(公告)号：US20140148576A1

    公开(公告)日：2014-05-29

    申请号：US14129028

    申请日：2012-06-22

    申请人： Jason Chin ,  Satpal Virdee

    发明人： Jason Chin ,  Satpal Virdee

    IPC分类号： C12P21/00 ,  C07C271/22 ,  C07C323/59 ,  C12N9/10

    CPC分类号： C12P21/00 ,  C07C271/22 ,  C07C323/59 ,  C07K2319/95 ,  C12N9/104 ,  C12N9/93 ,  C12Y601/01026

    摘要： The invention related to a tRNA synthetase capable of binding delta-substituted lysine, wherein said tRNA synthetase comprises amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS, wherein said sequence comprises 5 or fewer substitutions within the amino acid sequence corresponding to the amino acid sequence of at least L271 to Y349 of MbPyIRS; and wherein said synthetase comprises W at amino acid position 349 relative to MbPyIRS.

    摘要翻译： 本发明涉及能够结合δ-取代的赖氨酸的tRNA合成酶，其中所述tRNA合成酶包含对应于MbPyIRS的至少L271至Y349的氨基酸序列的氨基酸序列，其中所述序列在氨基酸内包含5个或更少个取代 对应于MbPyIRS的至少L271至Y349的氨基酸序列的序列; 并且其中所述合成酶在相对于MbPyIRS的氨基酸位置349处包含W。