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公开(公告)号：US08547533B2
公开(公告)日：2013-10-01
申请号：US13519737
申请日：2010-12-28
申请人： Jay R. Knutson , Gary L. Griffiths
发明人： Jay R. Knutson , Gary L. Griffiths
IPC分类号： G01C3/12
CPC分类号： C07K2/00 , C09B11/24 , C09B23/086 , G01N21/6428 , G01N21/6458 , G01N2021/6432 , G01N2021/6441 , Y10T436/21
摘要： Composite probes for super resolution optical techniques using super resolution via transiently activated quenchers (STAQ) include a donor moiety and an acceptor moiety joined by a linker, wherein the acceptor moiety, when excited by incident radiation, is excited to a state which, for example, absorbs in the donor emission region, such that the acceptor moiety in its excited state quenches at least a portion of the donor moiety emission. Other transiently activated quenching mechanisms and moieties could accomplish the same task by reducing donor population. Also disclosed are methods for irradiating a selected region of a target material including the composite probe, wherein the composite probe enables improved resolution by point spread function modification and/or nanoscale chemical reactions.
摘要翻译： 用于通过瞬时激活猝灭剂（STAQ）使用超分辨率的超分辨率光学技术的复合探针包括通过接头连接的供体部分和受体部分，其中受体部分在被入射辐射激发时被激发到例如 ，在受体发射区吸收，使得其激发态的受体部分猝灭供体部分发射的至少一部分。 其他瞬时激活的猝灭机制和部分可以通过减少供体群体来完成相同的任务。 还公开了用于照射包括复合探针的靶材料的选定区域的方法，其中复合探针通过点扩散功能修饰和/或纳米级化学反应提高了分辨率。
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公开(公告)号：US20120309045A1
公开(公告)日：2012-12-06
申请号：US13519737
申请日：2010-12-28
申请人： Jay R. Knutson , Gary L. Griffiths
发明人： Jay R. Knutson , Gary L. Griffiths
CPC分类号： C07K2/00 , C09B11/24 , C09B23/086 , G01N21/6428 , G01N21/6458 , G01N2021/6432 , G01N2021/6441 , Y10T436/21
摘要： Composite probes for super resolution optical techniques using super resolution via transiently activated quenchers (STAQ) include a donor moiety and an acceptor moiety joined by a linker, wherein the acceptor moiety, when excited by incident radiation, is excited to a state which, for example, absorbs in the donor emission region, such that the acceptor moiety in its excited state quenches at least a portion of the donor moiety emission. Other transiently activated quenching mechanisms and moieties could accomplish the same task by reducing donor population. Also disclosed are methods for irradiating a selected region of a target material including the composite probe, wherein the composite probe enables improved resolution by point spread function modification and/or nanoscale chemical reactions.
摘要翻译： 用于通过瞬时激活猝灭剂（STAQ）使用超分辨率的超分辨率光学技术的复合探针包括通过接头连接的供体部分和受体部分，其中受体部分在被入射辐射激发时被激发到例如 ，在受体发射区吸收，使得其激发态的受体部分猝灭供体部分发射的至少一部分。 其他瞬时激活的猝灭机制和部分可以通过减少供体群体来完成相同的任务。 还公开了用于照射包括复合探针的靶材料的选定区域的方法，其中复合探针通过点扩散功能修饰和/或纳米级化学反应提高了分辨率。
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3.发明授权Non-contact total emission detection method and system for multi-photon microscopy 有权非接触式全发射检测方法及多光子显微镜系统公开(公告)号：US08759792B2
公开(公告)日：2014-06-24
申请号：US13383248
申请日：2010-07-12
IPC分类号： H01J65/08
CPC分类号： G01N21/64 , G01N21/6458 , G02B21/02 , G02B21/084 , G02B21/16 , G02B2207/113 , G02B2207/114
摘要： A multi-photon microscope having an illumination source that transmits an illumination light into a housing having an objective lens arrangement for illuminating a sample disposed outside the housing and directing a first portion of emission light emitted from the sample to a detection system is disclosed. A light collection system is disposed proximate the objective lens arrangement for directing a second portion of emission light in a coaxial relationship with the first portion of emission light to the detection system such that substantially all of the emission light on, around and above the illumination region is detected.
摘要翻译： 公开了一种多光子显微镜，其具有将照明光透射到具有用于照亮设置在壳体外部的样本的物镜结构的壳体中并将从样本发射的发射光的第一部分引导到检测系统的照明光源。 光收集系统设置在物镜布置附近，用于将发射光的第二部分以与发射光的第一部分同轴的关系引导到检测系统，使得基本上所有的照射区域周围和上方的发射光 被检测到。
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4.发明申请NON-CONTACT TOTAL EMISSION DETECTION METHOD AND SYSTEM FOR MULTI-PHOTON MICROSCOPY 有权非接触式总发射检测方法及多光子显微镜系统公开(公告)号：US20130153788A1
公开(公告)日：2013-06-20
申请号：US13383248
申请日：2010-07-12
申请人： Jay R. Knutson , Christian A. Combs
发明人： Jay R. Knutson , Christian A. Combs
IPC分类号： G01N21/64
CPC分类号： G01N21/64 , G01N21/6458 , G02B21/02 , G02B21/084 , G02B21/16 , G02B2207/113 , G02B2207/114
摘要： A multi-photon microscope having an illumination source that transmits an illumination light into a housing having an objective lens arrangement for illuminating a sample disposed outside the housing and directing a first portion of emission light emitted from the sample to a detection system is disclosed. A light collection system is disposed proximate the objective lens arrangement for directing a second portion of emission light in a coaxial relationship with the first portion of emission light to the detection system such that substantially all of the emission light on, around and above the illumination region is detected.
摘要翻译： 公开了一种多光子显微镜，其具有将照明光透射到具有用于照亮设置在壳体外部的样本的物镜结构的壳体中并将从样本发射的发射光的第一部分引导到检测系统的照明光源。 光收集系统设置在物镜布置附近，用于将发射光的第二部分以与发射光的第一部分同轴的关系引导到检测系统，使得基本上所有的照射区域周围和上方的发射光 被检测到。
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5.发明授权Method and apparatus for imaging a physical parameter in turbid media using diffuse waves 失效使用DIFFUSE波形在涡轮介质中成像物理参数的方法和装置
公开(公告)号：US5203339A
公开(公告)日：1993-04-20
申请号：US722823
申请日：1991-06-28
申请人： Alexander Knuttel , Jay R. Knutson
发明人： Alexander Knuttel , Jay R. Knutson
IPC分类号： G01J1/04 , G01J9/02 , G01N21/41 , G01N21/45 , G01N21/47 , G01N21/65 , G01N21/76 , G01N21/85 , G01N27/447
CPC分类号： G01J1/04 , G01J1/0422 , G01J3/02 , G01J3/0208 , G01J3/0216 , G01N21/4795 , G01N27/44721 , G01J2009/0203 , G01N2021/1738 , G01N2021/1759 , G01N2021/178 , G01N2021/1782 , G01N2021/4173 , G01N2021/451 , G01N2021/4702 , G01N21/645 , G01N21/65 , G01N21/76 , G01N21/85 , G01N2201/0893
摘要： Imaging of a turbid object utilizes interference among the modulation wavefronts of a plurality of modulated light rays propagating through the object by diffusion and having predetermined phases relative to one another. A computer controlled phase and amplitude selecting device, such as a zone plate, is used to modulate light rays at appropriate phases in order to obtain constructive interference only at a predetermined portion of the object, including one or more preselected voxels. The rays reflected from (or diffusively transmitted through) the predetermined portion are received simultaneously at a detector, thus providing simultaneously all the data necessary to describe or image the portion. A single detector element may be used to detect the scattered reflected or transmitted light from the portion and to generate a signal representing the amplitude and phase characteristics for the modulation wavefront, thereby to provide absorption (and other) characteristics descriptive of the portion. An array of detectors may be used to detect the light from a plurality of individual voxels simultaneously and to provide such characteristics for each of the voxels. By dynamically controlling the phase and amplitude selecting device, the voxels selected for imaging are changed without mechanical scanning. Light rays having different frequencies may be modulated to provide complete absorption spectra for an arbitrarily selected portion of the object.
摘要翻译： 浑浊物体的成像利用通过扩散传播通过物体的多个调制光线的调制波前的相互干扰，并相对于彼此具有预定的相位。 使用计算机控制的相位和幅度选择装置（例如区域板）在适当的相位调制光线，以便仅在物体的预定部分（包括一个或多个预选的体素）处获得建设性的干扰。 从（或扩散传输）预定部分反射的光线在检测器处同时接收，从而同时提供描述或成像该部分所需的所有数据。 可以使用单个检测器元件来检测来自该部分的散射的反射或透射的光并且产生表示调制波前的幅度和相位特性的信号，从而提供描述该部分的吸收（和其它）特性。 可以使用一组检测器来同时检测来自多个单独体素的光，并为每个体素提供这样的特征。 通过动态地控制相位和幅度选择装置，选择用于成像的体素没有机械扫描而改变。 可以调制具有不同频率的光线，以便为对象的任意选择的部分提供完整的吸收光谱。
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公开(公告)号：US20120320458A1
公开(公告)日：2012-12-20
申请号：US13581107
申请日：2011-02-22
申请人： Jay R. Knutson
发明人： Jay R. Knutson
IPC分类号： G02B27/28
CPC分类号： G02B5/001 , G02B27/283 , G02B27/286 , G03F7/70225 , G03F7/70566
摘要： Polarizers and polarizing beam splitter include one or more pairs of axicons that are configured to separate an input beam into a radially polarized component and a tangentially (or azimuthally) polarized component. A second axicon pair can be provided to recombine the tangentially polarized component so as to provide a more uniform beam intensity. The radially polarized component can be reflected or otherwise directed so that one or both the radial and tangential components are available for use.
摘要翻译： 偏振器和偏振分束器包括一对或多对轴，其被配置为将输入光束分离成径向偏振分量和切向（或方位角）偏振分量。 可以提供第二轴对对，以重新组合切向极化分量，以便提供更均匀的束强度。 径向偏振分量可以被反射或以其他方式定向，使得径向和切向分量中的一个或两个可用于使用。
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公开(公告)号：US07667210B2
公开(公告)日：2010-02-23
申请号：US11979600
申请日：2007-11-06
IPC分类号： G01J1/58
CPC分类号： G02B21/06 , G02B2207/114
摘要： A multi-photon microscope has an illumination source, an objective lens unit arranged in an optical path of the illumination source, a first light collection system arranged to collect a first portion of light emitted from a sample when the sample is illuminated by light from the illumination source, and a second light collection system arranged to collect a second portion of light emitted from the sample when the sample is illuminated by light from the illumination source. The first portion of light when collected by the first light collection system and the second portion of light when collected by the second light collection system, together provide a means of collecting as much light from as many angles as possible emanating from an emitting point source. This collection scheme has the potential to approach the total emission collection of light from an emitting point source depending on the optical properties of the sample being imaged.
摘要翻译： 多光子显微镜具有照明源，布置在照明源的光路中的物镜单元，第一光收集系统，被布置成当样品被来自光源的光照射时，收集从样品发射的光的第一部分 照明源和第二光收集系统，其被布置成当样品被来自照明源的光照射时收集从样品发射的光的第二部分。 由第一光收集系统收集的第一部分光和第二光收集系统收集的第二部分光一起提供了从发射点源发出的尽可能多的角度收集尽可能多的光的装置。 该采集方案具有取决于正在成像的样品的光学性质来接近来自发射点源的光的总发射收集的潜力。
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8.发明授权公开(公告)号：US09448158B2
公开(公告)日：2016-09-20
申请号：US13983534
申请日：2012-02-03
CPC分类号： G01N21/01 , G01N21/6458 , G01N2021/6469 , G01N2201/08 , G02B6/241 , G02B21/16 , G02B21/26
摘要： Light collectors for fluorescence microscopy include slab light guides having a specimen volume such as a specimen well configured to retain a specimen. The specimen volume is bounded by a surface of the slab light guide that permits laterally propagating fluorescence to enter the slab light guide and propagate toward a detection or imaging system. Typically, the slab light guide has an elliptical perimeter and the sample volume is situated at a first focus of the elliptical perimeter. A reflector such as a conical reflector is situated at the second focus so as to direct the collected fluorescence to exit the slab light guide.
摘要翻译： 用于荧光显微镜的收集器包括具有样本体积的板状光导，例如准确地保持样本的样本。 样本体积由平板导光体的表面限制，允许横向传播的荧光进入平板光导并向检测或成像系统传播。 通常，平板导光体具有椭圆周长，并且样品体积位于椭圆周长的第一焦点。 诸如锥形反射器的反射器位于第二焦点处，以便引导所收集的荧光以离开平板光导。
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公开(公告)号：US09086509B2
公开(公告)日：2015-07-21
申请号：US13581107
申请日：2011-02-22
申请人： Jay R. Knutson
发明人： Jay R. Knutson
CPC分类号： G02B5/001 , G02B27/283 , G02B27/286 , G03F7/70225 , G03F7/70566
摘要： Polarizers and polarizing beam splitter include one or more pairs of axicons that are configured to separate an input beam into a radially polarized component and a tangentially (or azimuthally) polarized component. A second axicon pair can be provided to recombine the tangentially polarized component so as to provide a more uniform beam intensity. The radially polarized component can be reflected or otherwise directed so that one or both the radial and tangential components are available for use.
摘要翻译： 偏振器和偏振分束器包括一对或多对轴，其被配置为将输入光束分离成径向偏振分量和切向（或方位角）偏振分量。 可以提供第二轴对对，以重新组合切向极化分量，以便提供更均匀的束强度。 径向偏振分量可以被反射或以其他方式定向，使得径向和切向分量中的一个或两个可用于使用。
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10.发明申请公开(公告)号：US20130308126A1
公开(公告)日：2013-11-21
申请号：US13983534
申请日：2012-02-03
IPC分类号： G01N21/01
CPC分类号： G01N21/01 , G01N21/6458 , G01N2021/6469 , G01N2201/08 , G02B6/241 , G02B21/16 , G02B21/26
摘要： Light collectors for fluorescence microscopy include slab light guides having a specimen volume such as a specimen well configured to retain a specimen. The specimen volume is bounded by a surface of the slab light guide that permits laterally propagating fluorescence to enter the slab light guide and propagate toward a detection or imaging system. Typically, the slab light guide has an elliptical perimeter and the sample volume is situated at a first focus of the elliptical perimeter. A reflector such as a conical reflector is situated at the second focus so as to direct the collected fluorescence to exit the slab light guide.
摘要翻译： 用于荧光显微镜的收集器包括具有样本体积的板状光导，例如准确地保持样本的样本。 样本体积由平板导光体的表面限制，允许横向传播的荧光进入平板光导并向检测或成像系统传播。 通常，平板导光体具有椭圆周长，并且样品体积位于椭圆周长的第一焦点。 诸如锥形反射器的反射器位于第二焦点处，以便引导所收集的荧光以离开平板光导。
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