公开(公告)号：US20100093052A1

公开(公告)日：2010-04-15

申请号：US12514618

申请日：2007-11-14

申请人： Jeffrey J. Chalmers ,  Oscar R. Lara-Velasco ,  Xiaodong Tong ,  Maciej Zborowski ,  Lee R. Moore ,  Sherif S. Farag

发明人： Jeffrey J. Chalmers ,  Oscar R. Lara-Velasco ,  Xiaodong Tong ,  Maciej Zborowski ,  Lee R. Moore ,  Sherif S. Farag

IPC分类号： C12N13/00 ,  C12M1/42

CPC分类号： B03C1/288 ,  B03C2201/18 ,  B03C2201/26

摘要： Systems and methods are provided separating a first group of cells from a mixture of at least first and second groups of cells in a suspending fluid. The system includes an annular flow channel and at least one magnetic element positioned around the exterior of the annular flow channel as to provide a radially symmetric magnetic field around at least a portion of the annular flow channel. The at least one magnetic element is configured to provide a gradually increasing magnetic field along the axis of flow as to limit the maximum magnetic gradient within the annular flow channel. A pump is operatively connected to a terminal end of the annular flow channel to force the suspending fluid through the annular channel.

摘要翻译： 提供了将系统和方法从悬浮液中的至少第一组和第二组细胞的混合物中分离出第一组细胞。 该系统包括环形流动通道和围绕环形流动通道的外部定位的至少一个磁性元件，以便围绕环形流动通道的至少一部分提供径向对称的磁场。 至少一个磁性元件构造成沿流动轴线提供逐渐增加的磁场，以限制环形流动通道内的最大磁梯度。 泵可操作地连接到环形流动通道的终端以迫使悬浮液通过环形通道。

公开(公告)号：US06467630B1

公开(公告)日：2002-10-22

申请号：US09653432

申请日：2000-09-01

申请人： Maciej Zborowski ,  Jeff Chalmers ,  Lee R. Moore

发明人： Maciej Zborowski ,  Jeff Chalmers ,  Lee R. Moore

IPC分类号： B03B560

CPC分类号： B03C5/026 ,  B01D39/1692 ,  B01D39/18 ,  B01D49/00 ,  B01D57/02 ,  B01D61/00 ,  B01D61/14 ,  G01N27/44769 ,  G01N30/0005

摘要： The present invention provides apparatuses and methods for continuously separating particles of interest from a heterogeneous particle population. The heterogeneity can be based on, for example, magnetic susceptibility, particle size, thermal diffusion, phase solubility, and combinations of the preceding. Based on the heterogeneity, a separation force capable of exploiting the separand (i.e., particles subject to the separation process) is provided. The various embodiments of the present invention preferably employ an annular separation channel, appropriate separation force and flow compartments. In a first embodiment, an annular separation channel having semi-permeable inner and outer cylindrical walls is used to generate lateral convection forces. In a second embodiment of the present invention, an annular separation channel having heat conductive inner and outer cylindrical walls is used to generate thermal diffusion forces. In a third embodiment, an annular separation channel having non-permeable inner and outer cylindrical walls is used to generate a solubility difference separation force based on solubility coefficient differences between phases of flow compartments. In a fourth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate an electrophoretic separation force. In a fifth embodiment, an annular separation channel having electrically conductive inner and outer cylindrical walls is used to generate a dielectrophoretic separation force. Yet other embodiments of the present invention provide for the combination of separation forces including lateral convection, thermal diffusion, solubility difference, electrophoretic, dielectrophoretic, and magnetic separation forces.

摘要翻译： 本发明提供了用于连续分离感兴趣的颗粒与异质颗粒群体的装置和方法。 异质性可以基于例如磁化率，粒度，热扩散，相溶性和前述的组合。 基于异质性，提供能够利用分离的分离力（即，进行分离处理的颗粒）。 本发明的各种实施例优选地采用环形分离通道，适当的分离力和流动隔室。 在第一实施例中，使用具有半透的内圆柱形壁和外圆柱形壁的环形分隔通道产生横向对流力。 在本发明的第二实施例中，使用具有导热内壁和外圆柱壁的环形分隔通道产生热扩散力。 在第三实施例中，使用具有不可渗透的内圆柱形壁和外圆柱形壁的环形分离通道，以基于流动隔室的相位之间的溶解度系数差产生溶度差分离力。 在第四实施例中，使用具有导电内壁和外圆柱形壁的环形分隔通道产生电泳分离力。 在第五实施例中，使用具有导电内壁和外圆柱形壁的环形分隔通道产生介电电泳分离力。 本发明的其它实施例提供了分离力的组合，包括横向对流，热扩散，溶解度差异，电泳，介电电泳和磁力分离力。

公开(公告)号：US6120735A

公开(公告)日：2000-09-19

申请号：US352725

申请日：1999-07-13

申请人： Maciej Zborowski ,  Jeff Chalmers ,  Lee R. Moore

发明人： Maciej Zborowski ,  Jeff Chalmers ,  Lee R. Moore

IPC分类号： G01N15/14 ,  G01N33/49 ,  G01N33/00

CPC分类号： G01N33/491 ,  G01N2015/149

摘要： A method and apparatus for the fraction sorting of cells is provided. The invention include a dispensing device, aspirating device, flow channel and a magnetic assembly. A first fractional sorting device includes a dipole fractional cell sorter. The dipole fractional cell sorter includes large aspect ratio flow channel and a dipole magnetic assembly. A second fractional sorting device includes a quadrupole fractional cell sorter. The quadrupole fractional cell sorter includes an annular flow channel having inlet and outlet splitters, and a quadrupole magnetic assembly. The quadrupole magnetic assembly includes pole pieces having projecting members with convex surfaces and in is in physical communication with the annular flow chamber. The method of the present invention provides a flow through fractional cell sorting process which is based on the application of a magnetic force to cells having a range of magnetic labeling densities.

摘要翻译： 提供了用于细胞分级分选的方法和装置。 本发明包括分配装置，抽吸装置，流动通道和磁性组件。 第一分数分选装置包括偶极子分数细胞分选器。 偶极子分数细胞分选机包括大纵横比流动通道和偶极磁组件。 第二分数分类装置包括四极分数细胞分选器。 四极分级电池分选机包括具有入口和出口分流器以及四极磁组件的环形流动通道。 四极磁组件包括具有凸表面并具有与环形流动腔物理连通的突出构件的极片。 本发明的方法提供了一种通过分数细胞分选过程的流程，其基于将磁力应用于具有一定范围的磁标记密度的细胞。

公开(公告)号：US5968820A

公开(公告)日：1999-10-19

申请号：US806598

申请日：1997-02-26

申请人： Maciej Zborowski ,  Jeff Chalmers ,  Lee R. Moore

发明人： Maciej Zborowski ,  Jeff Chalmers ,  Lee R. Moore

IPC分类号： B03C1/035 ,  C12M1/42 ,  C12N13/00 ,  G01N15/14 ,  G01N33/543 ,  C12N5/00 ,  A01N1/02 ,  B01D35/06 ,  B03C1/00 ,  B07C5/02 ,  B07C9/00

CPC分类号： G01N33/54326 ,  B03C1/035 ,  C12M47/04 ,  C12N13/00 ,  G01N2015/149

摘要： A method for separating cells is presented. The method provides a flow through fractional cell sorting process that is based on the application of a magnetic force to cells having a range of magnetic labeling densities. Magnetically labeled cells are introduced into a flow channel. The cells are continuously fractionated into flow streams based on magnetic susceptibility by subjecting the cells in the flow channel to a magnetic field. The fractionated cells are recovered based on their response to the magnetic field. Methods employing dipole fractional sorters and quadrapole annular sorters are also presented.

摘要翻译： 提出了一种分离细胞的方法。 该方法提供了通过分数细胞分选过程的流程，其基于将磁力施加到具有一定范围的磁标记密度的细胞。 将磁性标记的细胞引入流动通道。 通过使流路中的细胞经受磁场，基于磁化率将细胞连续分馏成流动流。 基于它们对磁场的响应，分离的细胞被回收。 还提出了使用偶极子分数分拣机和四极环状分拣机的方法。