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    Sample preparation method incorporating an alkaline shock
    3.
    发明申请
    Sample preparation method incorporating an alkaline shock 有权

    公开(公告)号：US20060188912A1

    公开(公告)日：2006-08-24

    申请号：US11356613

    申请日：2006-02-17

    申请人： Kui Gao ,  Michael Becker ,  Wen Wu ,  Jeffrey Linnen

    发明人： Kui Gao ,  Michael Becker ,  Wen Wu ,  Jeffrey Linnen

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1006 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2527/119

    摘要： Method of preparing a biological sample appropriate for use in a subsequent in vitro nucleic acid amplification reaction. The method involves a rapid, transient exposure to alkaline conditions which can be achieved by mixing an alkaline solution with a pH-buffered solution that includes a detergent and the biological sample to be tested for the presence of particular nucleic acid species using in vitro amplification. The invented method advantageously can improve detection of some target nucleic acids without substantially compromising detectability of others. The method is particularly useful for simultaneously preparing RNA and DNA templates that can be used in multiplex amplification reactions.

    Compositions, methods and kits for detecting the nucleic acids of HIV-1 and HIV-2
    4.
    发明申请
    Compositions, methods and kits for detecting the nucleic acids of HIV-1 and HIV-2 有权
    用于检测HIV-1和HIV-2核酸的组合物，方法和试剂盒

    公开(公告)号：US20050153282A1

    公开(公告)日：2005-07-14

    申请号：US11015605

    申请日：2004-12-16

    申请人： Jeffrey Linnen ,  Wen Wu

    发明人： Jeffrey Linnen ,  Wen Wu

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/703 ,  C12Q2563/103 ,  C12Q2531/113 ,  C12Q2523/101

    摘要： Compositions, methods and kits for detecting the nucleic acids of HIV-1, HIV-2, or the combination of HIV-1 and HIV-2. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers, including cross-reacting hybridization probes and cross-reacting amplification primers, for detecting very low levels of viral nucleic acids.

    摘要翻译： 用于检测HIV-1，HIV-2或HIV-1和HIV-2的组合的核酸的组合物，方法和试剂盒。 特别描述的是可用作杂交探针和扩增引物（包括用于检测非常低水平的病毒核酸的交叉反应杂交探针和交叉反应扩增引物）的寡核苷酸。

    Polymer Foams With Treated Fillers, Fabrication Method And Products Using Same
    6.
    发明申请
    Polymer Foams With Treated Fillers, Fabrication Method And Products Using Same 有权
    具有处理填料的聚合物泡沫，使用相同的制造方法和产品

    公开(公告)号：US20070269647A1

    公开(公告)日：2007-11-22

    申请号：US11687475

    申请日：2007-03-16

    申请人： Wen Wu ,  David Dobreski

    发明人： Wen Wu ,  David Dobreski

    IPC分类号： B32B5/22 ,  B29C44/04 ,  C08J9/00

    CPC分类号： C08J9/0066 ,  Y10T428/249981 ,  Y10T428/249986 ,  Y10T428/249988 ,  Y10T428/249991 ,  Y10T428/249992 ,  Y10T428/249993

    摘要： The present invention provides for a polymer composite foam with a treated filler and methods for making the same. The polymer composite foam includes a polymer capable of being formed into a polymeric foam and a treated filler, wherein the polymer defines a plurality of cells dispersed therethrough and the treated filler is dispersed throughout the polymer. The filler is treated by techniques which exfoliate, delaminate or intercalate the filler particles into individual micro and/or nano size particulates and platelets. Ideally, the treated filler has a median particle size ranging from about 0.1 nm-10 μm. The treated filler enhances the rigidity, barrier properties, heat deflection temperature, nucleation characteristics, fire retardant characteristics and impact properties of the foam. The products fabricated from the polymer composite foam include containers, cups, sleeves, bags, plates, bowls, protective packaging, boards, sheets, void fillers, storageware, dinnerware and cookware. The present invention also provides for methods of fabricating the polymer composite foam.

    摘要翻译： 本发明提供一种具有处理填料的聚合物复合泡沫体及其制备方法。 聚合物复合泡沫体包括能够形成聚合物泡沫和经处理的填料的聚合物，其中聚合物限定了分散在其中的多个细胞，并且处理的填料分散在整个聚合物中。 填料通过将填料颗粒剥离，分层或嵌入到单独的微粒和/或纳米尺寸颗粒和血小板中的技术来处理。 理想地，处理过的填料的中值粒度为约0.1nm-10μm。 经处理的填料增强了泡沫的刚性，阻隔性能，热变形温度，成核特性，阻燃特性和冲击性能。 由聚合物复合泡沫制成的产品包括容器，杯子，袖子，袋子，盘子，碗，保护性包装，板，片，空隙填料，储存器皿，餐具和炊具。 本发明还提供了制造聚合物复合泡沫体的方法。

    Lens measuring method and device for determining decenter and tilt of the lens
    7.
    发明申请
    Lens measuring method and device for determining decenter and tilt of the lens 失效
    用于确定透镜的倾斜和倾斜的透镜测量方法和装置

    公开(公告)号：US20070201037A1

    公开(公告)日：2007-08-30

    申请号：US11513601

    申请日：2006-08-31

    申请人： Wen Wu ,  Kuo-Cheng Huang ,  Chien-Shing Lee ,  Jung-Ru Yu

    发明人： Wen Wu ,  Kuo-Cheng Huang ,  Chien-Shing Lee ,  Jung-Ru Yu

    IPC分类号： G01B11/02

    CPC分类号： G01B11/272

    摘要： A device for measuring a lens, comprising a first interferometer having a first optical axis and carried on a first adjustment base, a lens holder for holding the lens having a first surface having a first lens optical axis and a second surface having a second lens optical axis, and a platen having a sliding rail and carrying the first adjustment base and the lens holder thereon, wherein the lens holder is movable on the sliding rail, wherein each of the first adjustment base and the lens holder has a tetra-axis adjustment mechanism through which a relative positional relationship of the first optical axis of the first interferometer and the first lens optical axis of the first surface of the lens is adjustable.

    摘要翻译： 一种用于测量透镜的装置，包括具有第一光轴并承载在第一调节基座上的第一干涉仪，用于保持具有第一透镜光轴的第一表面的透镜的透镜保持器和具有第二透镜光学的第二表面 以及具有滑动导轨并在其上承载第一调节基座和透镜架的压板，其中透镜保持件可在滑轨上移动，其中第一调节基座和透镜保持器中的每一个具有四轴调节机构 第一干涉仪的第一光轴与透镜的第一表面的第一透镜光轴之间的相对位置关系可调整。

    Compositions, methods and kits for detecting the nucleic acids of HIV-1 and HIV-2
    8.
    发明授权
    Compositions, methods and kits for detecting the nucleic acids of HIV-1 and HIV-2 有权
    用于检测HIV-1和HIV-2核酸的组合物，方法和试剂盒

    公开(公告)号：US07255996B2

    公开(公告)日：2007-08-14

    申请号：US11015605

    申请日：2004-12-16

    申请人： Jeffrey M. Linnen ,  Wen Wu

    发明人： Jeffrey M. Linnen ,  Wen Wu

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/703 ,  C12Q2563/103 ,  C12Q2531/113 ,  C12Q2523/101

    摘要： Compositions, methods and kits for detecting the nucleic acids of HIV-1, HIV-2, or the combination of HIV-1 and HIV-2. Particularly described are oligonucleotides that are useful as hybridization probes and amplification primers, including cross-reacting hybridization probes and cross-reacting amplification primers, for detecting very low levels of viral nucleic acids.

    摘要翻译： 用于检测HIV-1，HIV-2或HIV-1和HIV-2的组合的核酸的组合物，方法和试剂盒。 特别描述的是可用作杂交探针和扩增引物（包括用于检测非常低水平的病毒核酸的交叉反应杂交探针和交叉反应扩增引物）的寡核苷酸。

    Method and system for tracking of a virtual electrode on a coronary sinus catheter in fluoroscopic images
    10.
    发明授权
    Method and system for tracking of a virtual electrode on a coronary sinus catheter in fluoroscopic images 有权
    用于在荧光镜图像中跟踪冠状静脉窦导管上的虚拟电极的方法和系统

    公开(公告)号：US08666477B2

    公开(公告)日：2014-03-04

    申请号：US13413721

    申请日：2012-03-07

    申请人： Wen Wu ,  Terrence Chen ,  Norbert Strobel ,  Gareth Funka-Lea ,  Dorin Comaniciu

    发明人： Wen Wu ,  Terrence Chen ,  Norbert Strobel ,  Gareth Funka-Lea ,  Dorin Comaniciu

    IPC分类号： A61B5/05

    CPC分类号： G06K9/64 ,  A61B6/12 ,  A61B6/487 ,  G06T7/248 ,  G06T7/277 ,  G06T2207/10121 ,  G06T2207/20081 ,  G06T2207/30021 ,  G06T2207/30048

    摘要： A method and system for detecting a virtual electrode (VE) on a coronary sinus (CS) catheter in a fluoroscopic image sequence is disclosed. User inputs indicating locations of CS catheter electrodes and a location of a VE are received. A catheter electrode model and a VE part model is initialized in a first frame of the fluoroscopic image sequence. The VE is tracked by detecting electrode position candidates and catheter body point candidates in the subsequent frames of the fluoroscopic image sequence using respective trained detectors, tracking the catheter electrode model in the subsequent frames based on the detected electrode position candidates, generating VE part hypotheses in the subsequent frames based on detection of the most proximal electrode (MPE) in each subsequent frame, calculating a probability score for each of the VE part hypotheses, and selecting an VE part hypothesis with the highest probability score.

    摘要翻译： 公开了一种用于在透视图像序列中检测冠状窦（CS）导管上的虚拟电极（VE）的方法和系统。 接收指示CS导管电极位置和VE位置的用户输入。 在荧光镜图像序列的第一帧中初始化导管电极模型和VE部分模型。 通过使用各自训练的检测器在荧光镜图像序列的后续帧中检测电极位置候选物和导管体点候选物来跟踪VE，基于检测到的电极位置候选，跟踪随后帧中的导管电极模型，生成VE部分假设 基于在每个后续帧中检测最近端电极（MPE）的后续帧，计算每个VE部分假设的概率分数，以及选择具有最高概率得分的VE部分假设。