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公开(公告)号：US20140075600A1
公开(公告)日：2014-03-13
申请号：US13516545
申请日：2010-12-15
申请人： Jihane Achkar , Theodorus Sonke , Martinus Julius Beekwilder , Hendrik Jan Bouwmeester , Hendrik Jan Bosch
发明人： Jihane Achkar , Theodorus Sonke , Martinus Julius Beekwilder , Hendrik Jan Bouwmeester , Hendrik Jan Bosch
CPC分类号： C12N9/88 , C12P5/007 , C12P7/26 , C12P7/40 , C12Y402/03073
摘要： The present invention relates to a valencene synthase, to a nucleic acid encoding such valencene synthase, to a host cell comprising said encoding nucleic acid sequence and to a method for preparing valencene, comprising converting farnesyl diphosphate to valencene in the presence of a valencene synthase according to the invention.
摘要翻译： 本发明涉及对于包含所述编码核酸序列的宿主细胞的编码这种戊烯合酶的核酸的戊烯合成酶和用于制备戊烯的方法，所述方法包括在戊烯合成酶存在下将法呢基二磷酸酯转化为戊烯， 涉及本发明。
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公开(公告)号：US09260709B2
公开(公告)日：2016-02-16
申请号：US13516545
申请日：2010-12-15
申请人： Jihane Achkar , Theodorus Sonke , Martinus Julius Beekwilder , Hendrik Jan Bouwmeester , Hendrik Jan Bosch
发明人： Jihane Achkar , Theodorus Sonke , Martinus Julius Beekwilder , Hendrik Jan Bouwmeester , Hendrik Jan Bosch
CPC分类号： C12N9/88 , C12P5/007 , C12P7/26 , C12P7/40 , C12Y402/03073
摘要： The present invention relates to a valencene synthase, to a nucleic acid encoding such valencene synthase, to a host cell comprising said encoding nucleic acid sequence and to a method for preparing valencene, comprising converting farnesyl diphosphate to valencene in the presence of a valencene synthase according to the invention.
摘要翻译： 本发明涉及对于包含所述编码核酸序列的宿主细胞的编码这种戊烯合酶的核酸的戊烯合成酶和用于制备戊烯的方法，所述方法包括在戊烯合成酶存在下将法呢基二磷酸酯转化为戊烯， 涉及本发明。
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3.发明申请MAMMALIAN-TYPE GLYCOSYLATION IN PLANTS BY EXPRESSION OF NON-MAMMALIAN GLYCOSYLTRANSFERASES 有权通过表达非马氏甘露糖衍生物的植物中的MAMMALIAN-TYPE GLYCOSYLATION公开(公告)号：US20110067146A1
公开(公告)日：2011-03-17
申请号：US12596063
申请日：2008-04-17
CPC分类号： C12N15/8257 , C07K2319/00 , C12N9/1051 , C12N15/8245 , C12N15/8246 , C12P21/005
摘要： The present invention relates to non-mammalian β-1,4-galactosyltransferases that can be used in their wild-type or in modified forms. The invention further relates to transformed plants and plant cells expressing non-mammalian β-1,4-galacto-syltransferase and methods to produce glycoproteins with altered and preferably mammalian-type glycosylation. The invention additionally provides nucleic acid molecules and expression vectors of non-mammalian β-1,4-galactosyltransferases.
摘要翻译： 本发明涉及可以以其野生型或修饰形式使用的非哺乳动物和β-蔗糖-1,4-半乳糖基转移酶。 本发明还涉及表达非哺乳动物和-1,4-半乳糖基转移酶的转化植物和植物细胞以及产生具有改变且优选哺乳动物型糖基化的糖蛋白的方法。 本发明另外提供了非哺乳动物和-1,4'-半乳糖基转移酶的核酸分子和表达载体。
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公开(公告)号：US20110030108A1
公开(公告)日：2011-02-03
申请号：US12838162
申请日：2010-07-16
CPC分类号： C12N9/1051 , C12N15/8257 , C12N15/8258
摘要： The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these. The invention provides a plant comprising a functional mammalian enzyme providing N-glycan biosynthesis that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.
摘要翻译： 本发明涉及用作生产重组生物药物蛋白质的成本效益和污染安全的工厂的转基因植物中的糖蛋白加工领域或包含它们的药物组合物。 本发明提供了一种植物，其包含通常不存在于植物中的提供N-聚糖生物合成的功能性哺乳动物酶，所述植物另外包含通常不存在于植物中的至少第二哺乳动物蛋白或其功能片段。
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5.发明申请公开(公告)号：US20120036596A9
公开(公告)日：2012-02-09
申请号：US12596063
申请日：2008-04-17
CPC分类号： C12N15/8257 , C07K2319/00 , C12N9/1051 , C12N15/8245 , C12N15/8246 , C12P21/005
摘要： The present invention relates to non-mammalian β-1,4-galactosyltransferases that can be used in their wild-type or in modified forms. The invention further relates to transformed plants and plant cells expressing non-mammalian β-1,4-galacto-syltransferase and methods to produce glycoproteins with altered and preferably mammalian-type glycosylation. The invention additionally provides nucleic acid molecules and expression vectors of non-mammalian β-1,4-galactosyltransferases.
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公开(公告)号：US20120010155A1
公开(公告)日：2012-01-12
申请号：US12980651
申请日：2010-12-29
IPC分类号： A61K38/14 , C07K14/575 , C12P21/00 , C07K16/00 , C07K14/475 , C07K1/14 , C07K14/00 , C07K14/71
CPC分类号： C12N15/8243 , C12N9/1051 , C12N15/8257 , C12P21/02
摘要： The invention relates to the field of glycoprotein processing in transgenic plants used as cost efficient and contamination safe factories for the production of recombinant biopharmaceutical proteins or pharmaceutical compositions comprising these glycoproteins. The invention provides a plant comprising a functional mammalian enzyme providing mammalian GnTIII that is normally not present in plants, said plant additionally comprising at least a second mammalian protein or functional fragment thereof that is normally not present in plants.
摘要翻译： 本发明涉及用作生产重组生物药物蛋白质或包含这些糖蛋白的药物组合物的成本效益和污染安全的工厂的转基因植物中的糖蛋白加工领域。 本发明提供了一种植物，其包含提供通常不存在于植物中的哺乳动物GnTIII的功能哺乳动物酶，所述植物另外包含至少一种通常不存在于植物中的哺乳动物蛋白质或其功能片段。
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公开(公告)号：US08058508B2
公开(公告)日：2011-11-15
申请号：US12553043
申请日：2009-09-02
申请人： Hendrikus Antonius Cornelis Bakker , Hendrik Jan Bosch , Dionisius Elisabeth Antonius Florack , Gerard Johan Adolph Rouwendal
发明人： Hendrikus Antonius Cornelis Bakker , Hendrik Jan Bosch , Dionisius Elisabeth Antonius Florack , Gerard Johan Adolph Rouwendal
CPC分类号： C12N9/1051 , C07K2317/13 , C07K2317/41 , C07K2319/02 , C12N15/8257 , C12N15/8258
摘要： The invention is directed to methods for optimizing glycan processing in organisms (and in particular, plants) so that a glycoprotein having complex type bi-antennary glycans and thus containing galactose residues on both arms and which are devoid of (or reduce in) xylose and fucose can be obtained. The invention is further directed to said glycoprotein obtained and host system comprising said protein.
摘要翻译： 本发明涉及用于优化生物（特别是植物）中的聚糖加工的方法，使得具有复合型双触角聚糖并因此在两臂上含有半乳糖残基并且没有（或减少）木糖的糖蛋白和 岩藻糖可以得到。 本发明还涉及所获得的所述糖蛋白和包含所述蛋白质的宿主系统。
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公开(公告)号：US20100227363A1
公开(公告)日：2010-09-09
申请号：US12601350
申请日：2008-05-23
申请人： Hendrik Jan Bosch , Alexander Ronald van der Krol , Maurice Gerard Leon Henquet , Dionisius Elisabeth Antonius Florack
发明人： Hendrik Jan Bosch , Alexander Ronald van der Krol , Maurice Gerard Leon Henquet , Dionisius Elisabeth Antonius Florack
CPC分类号： C12N15/8257 , C07K14/59 , C12N9/1051 , C12N15/8218 , C12N15/8245 , C12P21/005
摘要： The present invention relates to a method to produce proteins reduced in high mannose type glycans in a plant or plant cell by partially inhibiting the expression of the Alg3 gene in said plant or plant cell. Said partial inhibition can be provided for by inserting the Alg3 mutant gene from Arabidopsis as derived from the plant line Salk_040296c or by partially inhibiting the endogenous Alg3 gene by providing the plant or plant cell either with an antisense expression, a sense co-suppressing or with an RNA inhibition genetic construct. Optionally also tissue-specific expression of said inhibiting constructs can yield partial inhibition in a plant.
摘要翻译： 本发明涉及在植物或植物细胞中通过部分抑制Alg3基因在所述植物或植物细胞中的表达来产生在高甘露糖型聚糖中还原的蛋白质的方法。 所述部分抑制可以通过从植物系Salk_040296c引入来自拟南芥的Alg3突变体基因或通过将植物或植物细胞提供反义表达，感觉共抑制或与 RNA抑制遗传构建体。 任选地，所述抑制构建体的组织特异性表达可以在植物中产生部分抑制。
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公开(公告)号：US20080286297A1
公开(公告)日：2008-11-20
申请号：US10574888
申请日：2004-10-08
IPC分类号： A61K39/00 , C07K14/195 , C12P21/06 , A61K36/00 , C12N5/10
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/541 , A61K2039/55544 , C07K14/245 , C07K14/28 , C07K2319/00 , C12N15/8258 , C12N2770/24022
摘要： The invention relates to a protein complex for the delivery of an antigen to and across mucosal surfaces and the production of said complex in a host cell, such as a plant. Provided is a protein complex comprising at least two, preferably identical, subunits wherein at least one subunit is unaltered and at least one subunit is fused to a first molecule of interest and wherein the protein complex is able to interact with a cell surface receptor via said subunits. Also provided is a method for producing a protein complex according to the invention, comprising a) providing a host cell with a nucleotide sequence encoding an unaltered subunit and a nucleotide sequence encoding a molecule of interest, wherein at least one molecule of interest is fused to a subunit; b) culturing said host cell thereby allowing expression of said nucleotide sequences and allowing for assembly of the protein complex; c) isolating the complex; d) determining the binding of the complex to a cell surface receptor or to a molecule which mimics a cell surface receptor
摘要翻译： 本发明涉及用于将抗原递送至粘膜表面并跨过粘膜表面并在宿主细胞如植物中产生所述复合物的蛋白质复合物。 提供了包含至少两个，优选相同的亚基的蛋白质复合物，其中至少一个亚基是未改变的，并且至少一个亚基与第一目的分子融合，并且其中所述蛋白质复合物能够通过所述蛋白质复合体与细胞表面受体相互作用 亚单位。 还提供了一种用于生产根据本发明的蛋白质复合物的方法，包括：a）向宿主细胞提供编码未改变亚基的核苷酸序列和编码目的分子的核苷酸序列，其中至少一个目的分子融合到 一个亚单位 b）培养所述宿主细胞，从而允许表达所述核苷酸序列并允许蛋白质复合物的装配; c）分离复合物; d）确定复合物与细胞表面受体或模拟细胞表面受体的分子的结合
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公开(公告)号：US06780408B1
公开(公告)日：2004-08-24
申请号：US09668650
申请日：2000-09-22
IPC分类号： A01N6300
CPC分类号： A01N63/02 , C07K14/325 , C07K2319/00
摘要： The present invention provides, inter alia, a B.t. hybrid toxin fragment comprising at its C-terminus domain III of a first Cry protein, or a part of said domain or a protein substantially similar to said domain; and comprising at its N-terminus the N-terminal region of a second Cry protein, or a part of said region or a protein substantially similar to said region.
摘要翻译： 本发明尤其提供了一种B.t. 杂合毒素片段在其C末端结构域III处包含第一Cry蛋白或所述结构域的一部分或与所述结构域基本相似的蛋白质; 并且在其N-末端包含第二Cry蛋白的N-末端区域或所述区域的一部分或与所述区域基本相似的蛋白质。
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