公开(公告)号：US20100172881A1

公开(公告)日：2010-07-08

申请号：US12522127

申请日：2008-01-03

申请人： Joachim Hauber ,  Frank Buchholz ,  Ilona Hauber ,  Francis A. Stewart ,  Indrani Sarkar

发明人： Joachim Hauber ,  Frank Buchholz ,  Ilona Hauber ,  Francis A. Stewart ,  Indrani Sarkar

IPC分类号： A61K35/12 ,  C40B50/06 ,  C12N15/74 ,  C07H21/00 ,  C12N9/90 ,  C12N5/074 ,  A61K31/7088 ,  A61K38/52 ,  C12Q1/68 ,  A61P31/12

CPC分类号： C12N15/86 ,  C12N15/1058 ,  C12N2740/16043 ,  C12N2800/30

摘要： The present invention is directed to a method for preparing an expression vector encoding a tailored recombinase, wherein said tailored recombinase recombines asymmetric target sites within the LTR of proviral DNA of a retrovirus inserted into the genome of a host cell and is useful as means for excising the provirus from the genome of the host cell. The present invention further relates to an in vitro-method of optimising the treatment of a retroviral infection of a subject and to the use of tailored recombinases for the preparation of pharmaceutical compositions for reducing the viral load in a subjected infected by a retrovirus.

摘要翻译： 本发明涉及一种编码编码修饰重组酶的表达载体的方法，其中所述修整的重组酶重组插入到宿主细胞基因组中的逆转录病毒DNA的前病毒DNA的LTR内的不对称靶位点，并且可用作切除的手段 来自宿主细胞基因组的原病毒。 本发明还涉及优化对受试者的逆转录病毒感染的治疗的体外方法，以及使用调整的重组酶来制备用于减少由逆转录病毒感染的病毒的病毒载量的药物组合物。

公开(公告)号：US08871516B2

公开(公告)日：2014-10-28

申请号：US12522127

申请日：2008-01-03

申请人： Joachim Hauber ,  Frank Buchholz ,  Ilona Hauber ,  Francis A Stewart ,  Indrani Sarkar

发明人： Joachim Hauber ,  Frank Buchholz ,  Ilona Hauber ,  Francis A Stewart ,  Indrani Sarkar

IPC分类号： C12N15/87 ,  C12N15/10 ,  C12N15/86 ,  C12N15/00 ,  C12N9/90 ,  C12N9/00 ,  C07H21/02 ,  C07H21/04 ,  C12P21/04 ,  C12P21/06

CPC分类号： C12N15/86 ,  C12N15/1058 ,  C12N2740/16043 ,  C12N2800/30

摘要： The present invention is directed to a method for preparing an expression vector encoding a tailored recombinase, wherein said tailored recombinase recombines asymmetric target sites within the LTR of proviral DNA of a retrovirus inserted into the genome of a host cell and is useful as means for excising the provirus from the genome of the host cell. The present invention further relates to an in vitro-method of optimising the treatment of a retroviral infection of a subject and to the use of tailored recombinases for the preparation of pharmaceutical compositions for reducing the viral load in a subjected infected by a retrovirus.

摘要翻译： 本发明涉及一种编码编码修饰重组酶的表达载体的方法，其中所述修整的重组酶重组插入到宿主细胞基因组中的逆转录病毒DNA的前病毒DNA的LTR内的不对称靶位点，并且可用作切除的手段 来自宿主细胞基因组的原病毒。 本发明还涉及优化对受试者的逆转录病毒感染的治疗的体外方法，以及使用调整的重组酶来制备用于减少由逆转录病毒感染的病毒的病毒载量的药物组合物。

公开(公告)号：US20130164271A1

公开(公告)日：2013-06-27

申请号：US13698410

申请日：2011-05-27

申请人： Joachim Hauber ,  Jan Chemnitz ,  Frank Buchholz ,  Janet Chusainow

发明人： Joachim Hauber ,  Jan Chemnitz ,  Frank Buchholz ,  Janet Chusainow

IPC分类号： C12N9/12 ,  C12N15/10

CPC分类号： C12N9/1241 ,  C12N9/22 ,  C12N15/1058 ,  C12N2740/16022

摘要： The present invention relates to a method for preparing an expression vector encoding a tailored recombinase, which tailored recombinase is capable of recombining asymmetric target sequences within the long terminal repeat (LTR) of proviral DNA of a plurality of retrovirus strains inserted into the genome of a host cell, as well as to the obtained expression vector, cells transfected with this, expressed recombinase and pharmaceutical compositions comprising the expression vector, cells and/or recombinase. Pharmaceutical compositions are useful, e.g., in treatment and/or prevention of retrovirus infection. In particular, asymmetric target sequences present in a plurality of HIV strains are disclosed, as well as tailored recombinases capable of combining these sequences (Tre 3.0 and 4.0) and expression vectors encoding them.

公开(公告)号：US20130303594A1

公开(公告)日：2013-11-14

申请号：US13823431

申请日：2011-09-14

申请人： Dennis Kappei ,  Frank Buchholz ,  Matthias Mann ,  Falk Butter

发明人： Dennis Kappei ,  Frank Buchholz ,  Matthias Mann ,  Falk Butter

IPC分类号： C07K14/47

CPC分类号： C07K14/4703 ,  A61K38/00 ,  C07K14/47 ,  C12N15/113 ,  C12N2310/14

摘要： The invention relates to polynucleotides and polypeptides as defined in the claims for use in the treatment of a disorder associated with abnormal telomerase activity and/or abnormal telomere length in comparison to healthy subjects in a subject. Moreover, the present invention provides a method of identifying an agent capable of decreasing telomerase activity and/or telomere length, and a method of classifying a cancer as a telomerase-negative cancer, as defined in the claims.

摘要翻译： 本发明涉及权利要求中定义的多核苷酸和多肽，其用于治疗与受试者中的健康受试者相比，与端粒酶活性异常和/或异常端粒长度相关的病症。 此外，本发明提供了鉴定能够降低端粒酶活性和/或端粒长度的试剂的方法，以及如权利要求中所定义的将癌症分类为端粒酶阴性癌症的方法。

公开(公告)号：US07736851B2

公开(公告)日：2010-06-15

申请号：US10842534

申请日：2004-05-11

申请人： Francis Stewart ,  Youming Zhang ,  Frank Buchholz

发明人： Francis Stewart ,  Youming Zhang ,  Frank Buchholz

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/10 ,  C12N15/902

摘要： The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.

摘要翻译： 本发明涉及使用在至少两个DNA分子之间的recE / recT介导的同源重组机制克隆DNA分子的方法，其中一个DNA分子是圆形或线性DNA分子，第二DNA分子是环状DNA分子，第二DNA 分子含有与第一个DNA分子具有序列同源性的两个区域。 还描述了感受态细胞和载体。

公开(公告)号：US20060208739A1

公开(公告)日：2006-09-21

申请号：US10553619

申请日：2004-03-17

申请人： Karsten Schiller ,  Frank Buchholz

发明人： Karsten Schiller ,  Frank Buchholz

IPC分类号： G01N27/416

CPC分类号： G01R31/3693 ,  F02D2200/503 ,  F02N11/0862 ,  F02N2200/042 ,  F02N2200/061 ,  F02N2200/062 ,  F02N2300/2006 ,  F02N2300/2008

摘要： A device for predicting the starting ability of a vehicle having an internal combustion engine and a starter which is supplied with electrical power by a vehicle battery. The starting ability of the vehicle can be determined particularly easily when a starting current characteristics map is stored in a device of the prediction system from which a starting current is determined under consideration of the state of charge of the battery after a predefined time period and the starting ability of the vehicle is determined from this via a prediction device.

摘要翻译： 一种用于预测具有内燃机和起动机的车辆的启动能力的装置，其由车辆电池供电。 当启动电流特性图存储在预测系统的装置中时，可以特别容易地确定车辆的启动能力，在该预测系统的装置中，在预定时间段之后考虑到电池的充电状态来确定起动电流，并且 通过预测装置从其确定车辆的起动能力。

公开(公告)号：US20130159385A1

公开(公告)日：2013-06-20

申请号：US13329055

申请日：2011-12-16

申请人： Alexander Ocher ,  Frank Buchholz ,  Werner Daehn ,  Wu Cao ,  Ben Hofmans

发明人： Alexander Ocher ,  Frank Buchholz ,  Werner Daehn ,  Wu Cao ,  Ben Hofmans

IPC分类号： G06F15/16

CPC分类号： G06F9/547 ,  G06F21/629 ,  G06F2209/542

摘要： Methods, systems, and apparatuses for processing function calls using a hooking routines to perform pre-execution tasks are disclosed. A server computer receives a function call including a hooking routine and a request to run a particular function from a client computer. Based on a function group identifier and the credentials of the user or client computer, the server computer can perform various pre-execution tasks. The tasks can be common to all functions in the function group or be customized Both pre-execution tasks can be stored remotely from the client computer, so that updates to the pre-execution task can be made without the need to update computer readable code stored on the client computer. If the pre-execution tasks, such as an authorization, passes, then the server computer can execute the requested function, the server computer can reject or abort the requested function.

摘要翻译： 公开了使用挂钩例程处理函数调用以执行预执行任务的方法，系统和装置。 服务器计算机接收包括挂接例程和从客户端计算机运行特定功能的请求的功能调用。 基于功能组标识符和用户或客户端计算机的凭据，服务器计算机可以执行各种预执行任务。 这些任务对于功能组中的所有功能可以是通用的，或者可以被定制。两个预执行任务可以从客户端计算机远程存储，从而可以对预执行任务进行更新，而无需更新存储的计算机可读代码 在客户端计算机上。 如果预执行任务（例如授权）通过，则服务器计算机可以执行所请求的功能，服务器计算机可以拒绝或中止所请求的功能。

公开(公告)号：US07528570B2

公开(公告)日：2009-05-05

申请号：US10553619

申请日：2004-03-17

申请人： Karsten Schiller ,  Frank Buchholz

发明人： Karsten Schiller ,  Frank Buchholz

IPC分类号： H01M10/46

CPC分类号： G01R31/3693 ,  F02D2200/503 ,  F02N11/0862 ,  F02N2200/042 ,  F02N2200/061 ,  F02N2200/062 ,  F02N2300/2006 ,  F02N2300/2008

摘要： A device for predicting the starting ability of a vehicle having an internal combustion engine and a starter which is supplied with electrical power by a vehicle battery. The starting ability of the vehicle can be determined particularly easily when a starting current characteristics map is stored in a device of the prediction system from which a starting current is determined under consideration of the state of charge of the battery after a predefined time period and the starting ability of the vehicle is determined from this via a prediction device.

摘要翻译： 一种用于预测具有内燃机和起动机的车辆的启动能力的装置，其由车辆电池供电。 当启动电流特性图存储在预测系统的装置中时，可以特别容易地确定车辆的启动能力，在该预测系统的装置中，在预定时间段之后考虑到电池的充电状态来确定起动电流，并且 通过预测装置从其确定车辆的起动能力。

公开(公告)号：US06787316B2

公开(公告)日：2004-09-07

申请号：US10231013

申请日：2002-08-30

申请人： Francis Stewart ,  Youming Zhang ,  Frank Buchholz

发明人： Francis Stewart ,  Youming Zhang ,  Frank Buchholz

IPC分类号： C12Q168

CPC分类号： C12N15/10 ,  C12N15/902

摘要： The invention relates to methods for cloning DNA molecules using recE/recT-mediated homologous recombination mechanism between at least two DNA molecules where one DNA molecule is a circular or linear DNA molecule and the second DNA molecule is a circular DNA molecule, and the second DNA molecule contains two regions with sequence homology to the first DNA molecule. Competent cells and vectors are also described.

摘要翻译： 本发明涉及使用在至少两个DNA分子之间的recE / recT介导的同源重组机制克隆DNA分子的方法，其中一个DNA分子是圆形或线性DNA分子，第二DNA分子是环状DNA分子，第二DNA 分子含有与第一个DNA分子具有序列同源性的两个区域。 还描述了感受态细胞和载体。

公开(公告)号：US08819155B2

公开(公告)日：2014-08-26

申请号：US13329055

申请日：2011-12-16

申请人： Alexander Ocher ,  Frank Buchholz ,  Werner Daehn ,  Wu Cao ,  Ben Hofmans

发明人： Alexander Ocher ,  Frank Buchholz ,  Werner Daehn ,  Wu Cao ,  Ben Hofmans

IPC分类号： G06F15/16

CPC分类号： G06F9/547 ,  G06F21/629 ,  G06F2209/542

摘要： Methods, systems, and apparatuses for processing function calls using a hooking routines to perform pre-execution tasks are disclosed. A server computer receives a function call including a hooking routine and a request to run a particular function from a client computer. Based on a function group identifier and the credentials of the user or client computer, the server computer can perform various pre-execution tasks. The tasks can be common to all functions in the function group or be customized Both pre-execution tasks can be stored remotely from the client computer, so that updates to the pre-execution task can be made without the need to update computer readable code stored on the client computer. If the pre-execution tasks, such as an authorization, passes, then the server computer can execute the requested function, the server computer can reject or abort the requested function.

摘要翻译： 公开了使用挂钩例程处理函数调用以执行预执行任务的方法，系统和装置。 服务器计算机接收包括挂接例程和从客户端计算机运行特定功能的请求的功能调用。 基于功能组标识符和用户或客户端计算机的凭据，服务器计算机可以执行各种预执行任务。 这些任务对于功能组中的所有功能可以是通用的，或者可以被定制。两个预执行任务可以从客户端计算机远程存储，从而可以对预执行任务进行更新，而无需更新存储的计算机可读代码 在客户端计算机上。 如果预执行任务（例如授权）通过，则服务器计算机可以执行所请求的功能，服务器计算机可以拒绝或中止所请求的功能。