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17 条记录 (耗时 0.021 秒)

    Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof
    1.
    发明授权
    Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof 失效
    核苷三磷酸依赖性1-甲基乙内酰脲酶，其获得方法及其用途

    公开(公告)号：US4816393A

    公开(公告)日：1989-03-28

    申请号：US704712

    申请日：1985-02-25

    申请人： Joachim Siedel ,  Rolf Deeg ,  Albert Roder ,  Joachim Ziegenhorn ,  Hans Mollering ,  Helmgard Gauhl

    发明人： Joachim Siedel ,  Rolf Deeg ,  Albert Roder ,  Joachim Ziegenhorn ,  Hans Mollering ,  Helmgard Gauhl

    IPC分类号： C12N9/14 ,  C12N9/86 ,  C12Q1/34 ,  C12R1/01 ,  C12R1/06 ,  C12R1/13 ,  C12R1/265 ,  G01N33/70 ,  C12Q1/26 ,  C12Q1/32

    CPC分类号： C12N9/86 ,  C12Q1/34 ,  G01N33/70 ,  G01N2333/986 ,  Y10S435/81

    摘要： The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.

    摘要翻译： 本发明提供1-甲基乙内酰脲酶，其在核苷三磷酸和多价金属离子的存在下水解1-甲基乙内酰脲。 本发明还提供了获得1-甲基乙内酰脲酶和含有它的试剂的方法。 此外，本发明提供了通过将肌酐与肌酐去酰亚胺酶（EC 3.5.4.21）转化为1-甲基乙内酰脲来测定肌酸酐的方法，后者在核苷三磷酸存在下用1-甲基乙内酰脲酶水解， 测定（a）由N-甲基乙内酰脲与N-氨基甲酰基肌氨酰氨基水解酶形成的水解产物（肌氨酸形成），并用肌氨酸氧化酶或肌氨酸脱氢酶检测肌氨酸，或（b）同时形成的二磷酸核苷。

    Determination of NAD(P)H or salicylate
    3.
    发明授权
    Determination of NAD(P)H or salicylate 失效
    测定NAD（P）H或水杨酸

    公开(公告)号：US4416983A

    公开(公告)日：1983-11-22

    申请号：US328312

    申请日：1981-12-07

    申请人： Albert Roder ,  Joachim Siedel ,  Hans Mollering ,  Hans Seidel ,  Helmgard Gauhl

    发明人： Albert Roder ,  Joachim Siedel ,  Hans Mollering ,  Hans Seidel ,  Helmgard Gauhl

    IPC分类号： G01N33/66 ,  C12Q1/00 ,  C12Q1/26 ,  C12N9/02 ,  C12Q1/14 ,  C12Q1/32 ,  C12Q1/38 ,  C12Q1/40 ,  C12Q1/54 ,  C12Q1/56

    CPC分类号： C12Q1/26 ,  C12Q1/008 ,  Y10S435/805 ,  Y10S435/81

    摘要： The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically.The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.

    摘要翻译： 本发明提供了测定NAD（P）H或水杨酸盐的方法，其中在NAD（P）H依赖性反应中，水杨酸盐被水杨酸羟化酶脱羧，并且着色材料由 通过与合适的着色材料组分的氧化偶联来存在酪氨酸酶，形成的有色材料然后被光度测定。 本发明还提供了用于测定NADH或NADPH的试剂，其中含有水杨酸盐，显色腙或胺，水杨酸羟化酶，酪氨酸酶和缓冲液，以及用于测定水杨酸盐的试剂，其中它含有NAD（P ）H，显色腙或胺，水杨酸羟化酶，酪氨酸酶和缓冲液。

    Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide
    6.
    发明授权
    Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide 失效
    改进过氧化氢定量色谱法的方法和试剂

    公开(公告)号：US5094943A

    公开(公告)日：1992-03-10

    申请号：US147576

    申请日：1988-01-19

    申请人： Joachim Siedel ,  Albert Roder ,  Joachim Ziegenhorn

    发明人： Joachim Siedel ,  Albert Roder ,  Joachim Ziegenhorn

    IPC分类号： C12Q1/26 ,  C12Q1/28

    CPC分类号： C12Q1/28 ,  C12Q1/26

    摘要： The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and measurement of the colored material formed, wherein superoxide dismutase (E.C. 1.15.1.1) is added to the reagent solution.The present invention also provides a reagent for the improved colorimetric determination of hydrogen peroxide, comprising a hydrogen peroxide-producing oxidase, a chromogenic system, a buffer and optionally adjuvant enzymes, wherein it also contains superoxide dismutase.

    摘要翻译： 本发明提供了通过添加显色系统和形成的有色材料的测量来改进由产生过氧化氢的氧化酶形成的过氧化氢的比色测定方法，其中将超氧化物歧化酶（EC 1.15.1.1）加入到 试剂溶液。 本发明还提供了用于改进过氧化氢比色测定的试剂，其包含产生过氧化氢的氧化酶，显色系统，缓冲液和任选的辅助酶，其中还含有超氧化物歧化酶。

    NADH Peroxidase for hydrogen peroxide determination
    7.
    发明授权
    NADH Peroxidase for hydrogen peroxide determination 失效
    NADH过氧化物酶测定过氧化氢

    公开(公告)号：US4186052A

    公开(公告)日：1980-01-29

    申请号：US811422

    申请日：1977-06-29

    申请人： Albert Roder ,  Hans Mollering ,  Wolfgang Gruber ,  Klaus Beaucamp ,  Hans Seidel ,  Peter Stahl ,  Detlef von Hoerschelmann

    发明人： Albert Roder ,  Hans Mollering ,  Wolfgang Gruber ,  Klaus Beaucamp ,  Hans Seidel ,  Peter Stahl ,  Detlef von Hoerschelmann

    IPC分类号： C12N9/08 ,  C12Q1/28 ,  G01N33/50 ,  C07G7/02 ,  C12D13/10 ,  G01N31/14

    CPC分类号： C12N9/0065 ,  C12Q1/28

    摘要： A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.Mto hydrogen peroxide of 2.8.times.10.sup.-5 M andto reduced nicotinamide-adenine-dinucleotide of 1.7.times.10.sup.-5 M,measured at 25.degree. C. in 0.2M tris buffer of pH 6.0, containing 0.1M potassium acetate. The peroxidase can be prepared by liberating the enzyme from Streptococcus faecalis ATCC 8043 by digestion or by treatment with a surface-active agent and isolating same from the enzyme solution obtained. Hydrogen peroxide is determined in a simple reaction or in a coupled reaction with a specific oxidase by contacting the said peroxidase with the H.sub.2 O.sub.2 producing reaction mixture at a pH of from 6.0 to 9.0 and measuring the change of extinction as a measure of H.sub.2 O.sub.2 and of the concentration of nicotinamide-adenine-dinucleotide initially present in said reaction mixture.

    摘要翻译： 提供了一种用于还原型烟酰胺 - 腺嘌呤二核苷酸的新型过氧化物酶，其过氧化物酶用过氧化氢氧化还原型烟酰胺 - 腺嘌呤二核苷酸，得到烟酰胺 - 腺嘌呤二核苷酸和水，其特征在于具有2.8x10-5M的过氧化氢的米氏常数KM， 降低了1.7×10-5M的烟酰胺 - 腺嘌呤二核苷酸，在25℃下，在含有0.1M醋酸钾的0.2M Tris缓冲液pH6.0中测定。 过氧化物酶可以通过消化或通过用表面活性剂处理从粪链球菌ATCC 8043中释放酶制备，并从获得的酶溶液中分离。 过氧化氢在简单的反应或与特定氧化酶的偶联反应中通过使所述过氧化物酶与产生H 2 O 2的反应混合物在6.0至9.0的pH下接触并测定作为H 2 O 2和 最初存在于所述反应混合物中的烟酰胺 - 腺嘌呤二核苷酸的浓度。

    Process for specific determination of cholesterol in the presence of the HDL fraction in serum
    8.
    发明授权
    Process for specific determination of cholesterol in the presence of the HDL fraction in serum 失效
    在血清中HDL级分存在下特异性测定胆固醇的方法

    公开(公告)号：US4544630A

    公开(公告)日：1985-10-01

    申请号：US468792

    申请日：1983-02-22

    申请人： Joachim Ziegenhorn ,  Albert Roder ,  Knut Bartl ,  Gunter Wehmeyer

    发明人： Joachim Ziegenhorn ,  Albert Roder ,  Knut Bartl ,  Gunter Wehmeyer

    IPC分类号： C12Q1/26 ,  C12Q1/28 ,  C12Q1/44 ,  C12Q1/60

    CPC分类号： C12Q1/60

    摘要： The present invention provides a process for the specific determination of the cholesterol of the LDL fraction in the presence of the HDL fraction of the lipoproteins of serum by the action of cholesterol esterase for the liberation of the cholesterol and oxidation of the liberated cholesterol with cholesterol oxidase and oxygen with the formation of hydrogen peroxide and cholestenone and kinetic measurement of the change of one of the reaction components of the oxidase reaction, especially the formation of hydrogen peroxide, wherein the measurement is carried out in a predetermined period of time, the reaction solution having a tenside concentration of 0.01 to 1.5 mmol/liter, a cholesterol esterase concentration of 0.1 to 30 U/ml. and a pH value of 6.5 to 8.0.The present invention also provides a reagent for carrying out this process.

    摘要翻译： 本发明提供了一种在胆固醇酯酶的作用下，通过胆固醇释放和胆固醇氧化酶氧化释放的胆固醇，在血清脂蛋白的HDL部分存在下，特异性测定LDL级分的胆固醇的方法 和氧气，形成过氧化氢和胆甾烯酮，动力学测量氧化酶反应的一种反应成分的变化，特别是过氧化氢的形成，其中测量在预定时间内进行，反应溶液 其表面活性剂浓度为0.01〜1.5mmol / l，胆固醇酯酶浓度为0.1〜30U / ml。 pH值为6.5〜8.0。 本发明还提供了一种用于进行该方法的试剂。