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11 条记录 (耗时 0.018 秒)

    Determination of NAD(P)H or salicylate
    1.
    发明授权
    Determination of NAD(P)H or salicylate 失效
    测定NAD（P）H或水杨酸

    公开(公告)号：US4416983A

    公开(公告)日：1983-11-22

    申请号：US328312

    申请日：1981-12-07

    申请人： Albert Roder ,  Joachim Siedel ,  Hans Mollering ,  Hans Seidel ,  Helmgard Gauhl

    发明人： Albert Roder ,  Joachim Siedel ,  Hans Mollering ,  Hans Seidel ,  Helmgard Gauhl

    IPC分类号： G01N33/66 ,  C12Q1/00 ,  C12Q1/26 ,  C12N9/02 ,  C12Q1/14 ,  C12Q1/32 ,  C12Q1/38 ,  C12Q1/40 ,  C12Q1/54 ,  C12Q1/56

    CPC分类号： C12Q1/26 ,  C12Q1/008 ,  Y10S435/805 ,  Y10S435/81

    摘要： The present invention provides a process for the determination of NAD(P)H or of salicylate, wherein, in a NAD(P)H-dependent reaction, salicylate is decarboxylated by salicylate hydroxylase and a colored material is formed from the decarboxylation product in the presence of tyrosinase by oxidative coupling with an appropriate colored material component, the colored material formed then being determined photometrically.The present invention also provides a reagent for the determination of NADH or NADPH, wherein it contains salicylate, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer, as well as a reagent for the determination of salicylate, wherein it contains NAD(P)H, a chromogenic hydrazone or amine, salicylate hydroxylase, tyrosinase and buffer.

    摘要翻译： 本发明提供了测定NAD（P）H或水杨酸盐的方法，其中在NAD（P）H依赖性反应中，水杨酸盐被水杨酸羟化酶脱羧，并且着色材料由 通过与合适的着色材料组分的氧化偶联来存在酪氨酸酶，形成的有色材料然后被光度测定。 本发明还提供了用于测定NADH或NADPH的试剂，其中含有水杨酸盐，显色腙或胺，水杨酸羟化酶，酪氨酸酶和缓冲液，以及用于测定水杨酸盐的试剂，其中它含有NAD（P ）H，显色腙或胺，水杨酸羟化酶，酪氨酸酶和缓冲液。

    Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof
    2.
    发明授权
    Nucleoside triphosphate-dependent 1-methylhydantoinase, a process for obtaining it and the use thereof 失效
    核苷三磷酸依赖性1-甲基乙内酰脲酶，其获得方法及其用途

    公开(公告)号：US4816393A

    公开(公告)日：1989-03-28

    申请号：US704712

    申请日：1985-02-25

    申请人： Joachim Siedel ,  Rolf Deeg ,  Albert Roder ,  Joachim Ziegenhorn ,  Hans Mollering ,  Helmgard Gauhl

    发明人： Joachim Siedel ,  Rolf Deeg ,  Albert Roder ,  Joachim Ziegenhorn ,  Hans Mollering ,  Helmgard Gauhl

    IPC分类号： C12N9/14 ,  C12N9/86 ,  C12Q1/34 ,  C12R1/01 ,  C12R1/06 ,  C12R1/13 ,  C12R1/265 ,  G01N33/70 ,  C12Q1/26 ,  C12Q1/32

    CPC分类号： C12N9/86 ,  C12Q1/34 ,  G01N33/70 ,  G01N2333/986 ,  Y10S435/81

    摘要： The present invention provides 1-methylhydantoinase, which hydrolyses 1-methylhydantoin in the presence of a nucleoside triphosphate and of polyvalent metal ions.The present invention also provides a process for obtaining 1-methylhydantoinase and a reagent containing it.Furthermore, the present invention provides a process for the determination of creatinine by the conversion of the creatinine with creatinine deiminase (E.C. 3.5.4.21) into 1-methylhydantoin, hydrolysis of the latter with the 1-methylhydantoinase in the presence of nucleoside triphosphate and of polyvalent metal ions and determination(a) of the hydrolysis product formed from 1-methylhydantoin with N-carbamoylsarcosinamidohydrolase with formation of sarcosine and detection of the sarcosine with sarcosine oxidase or sarcosine dehydrogenase or(b) of the simultaneously formed nucleoside diphosphate.

    摘要翻译： 本发明提供1-甲基乙内酰脲酶，其在核苷三磷酸和多价金属离子的存在下水解1-甲基乙内酰脲。 本发明还提供了获得1-甲基乙内酰脲酶和含有它的试剂的方法。 此外，本发明提供了通过将肌酐与肌酐去酰亚胺酶（EC 3.5.4.21）转化为1-甲基乙内酰脲来测定肌酸酐的方法，后者在核苷三磷酸存在下用1-甲基乙内酰脲酶水解， 测定（a）由N-甲基乙内酰脲与N-氨基甲酰基肌氨酰氨基水解酶形成的水解产物（肌氨酸形成），并用肌氨酸氧化酶或肌氨酸脱氢酶检测肌氨酸，或（b）同时形成的二磷酸核苷。

    NADH Peroxidase for hydrogen peroxide determination
    5.
    发明授权
    NADH Peroxidase for hydrogen peroxide determination 失效
    NADH过氧化物酶测定过氧化氢

    公开(公告)号：US4186052A

    公开(公告)日：1980-01-29

    申请号：US811422

    申请日：1977-06-29

    申请人： Albert Roder ,  Hans Mollering ,  Wolfgang Gruber ,  Klaus Beaucamp ,  Hans Seidel ,  Peter Stahl ,  Detlef von Hoerschelmann

    发明人： Albert Roder ,  Hans Mollering ,  Wolfgang Gruber ,  Klaus Beaucamp ,  Hans Seidel ,  Peter Stahl ,  Detlef von Hoerschelmann

    IPC分类号： C12N9/08 ,  C12Q1/28 ,  G01N33/50 ,  C07G7/02 ,  C12D13/10 ,  G01N31/14

    CPC分类号： C12N9/0065 ,  C12Q1/28

    摘要： A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.Mto hydrogen peroxide of 2.8.times.10.sup.-5 M andto reduced nicotinamide-adenine-dinucleotide of 1.7.times.10.sup.-5 M,measured at 25.degree. C. in 0.2M tris buffer of pH 6.0, containing 0.1M potassium acetate. The peroxidase can be prepared by liberating the enzyme from Streptococcus faecalis ATCC 8043 by digestion or by treatment with a surface-active agent and isolating same from the enzyme solution obtained. Hydrogen peroxide is determined in a simple reaction or in a coupled reaction with a specific oxidase by contacting the said peroxidase with the H.sub.2 O.sub.2 producing reaction mixture at a pH of from 6.0 to 9.0 and measuring the change of extinction as a measure of H.sub.2 O.sub.2 and of the concentration of nicotinamide-adenine-dinucleotide initially present in said reaction mixture.

    摘要翻译： 提供了一种用于还原型烟酰胺 - 腺嘌呤二核苷酸的新型过氧化物酶，其过氧化物酶用过氧化氢氧化还原型烟酰胺 - 腺嘌呤二核苷酸，得到烟酰胺 - 腺嘌呤二核苷酸和水，其特征在于具有2.8x10-5M的过氧化氢的米氏常数KM， 降低了1.7×10-5M的烟酰胺 - 腺嘌呤二核苷酸，在25℃下，在含有0.1M醋酸钾的0.2M Tris缓冲液pH6.0中测定。 过氧化物酶可以通过消化或通过用表面活性剂处理从粪链球菌ATCC 8043中释放酶制备，并从获得的酶溶液中分离。 过氧化氢在简单的反应或与特定氧化酶的偶联反应中通过使所述过氧化物酶与产生H 2 O 2的反应混合物在6.0至9.0的pH下接触并测定作为H 2 O 2和 最初存在于所述反应混合物中的烟酰胺 - 腺嘌呤二核苷酸的浓度。

    Method and composition for the determination of glycerol
    9.
    发明授权
    Method and composition for the determination of glycerol 失效
    测定甘油的方法和组成

    公开(公告)号：US4273870A

    公开(公告)日：1981-06-16

    申请号：US054728

    申请日：1979-07-05

    申请人： Hans Mollering ,  Siegfried Looser

    发明人： Hans Mollering ,  Siegfried Looser

    IPC分类号： G01N33/50 ,  C12Q1/32 ,  G01N33/52

    CPC分类号： C12Q1/32

    摘要： Method and composition for the determination of glycerol using glycerin dehydrogenase, an electron transfer agent, NAD and a tetrazolium salt and, in addition, at least one microgram of zinc, in the form of a zinc salt, per 50 U of glycerol dehydrogenase.

    摘要翻译： 用于使用甘油脱氢酶，电子转移剂，NAD和四唑鎓盐测定甘油的方法和组合物，以及每50U甘油脱氢酶至少1微克锌盐形式的锌。

    Cloned N-Methylhydantoinase
    10.
    发明授权
    Cloned N-Methylhydantoinase 失效
    克隆N-甲基乙内酰脲酶

    公开(公告)号：US5432070A

    公开(公告)日：1995-07-11

    申请号：US258614

    申请日：1994-06-10

    申请人： Gunther Schumacher ,  Helmut Burtscher ,  Hans Mollering

    发明人： Gunther Schumacher ,  Helmut Burtscher ,  Hans Mollering

    IPC分类号： C12N1/21 ,  C12N9/86 ,  C12N15/09 ,  C12N15/54 ,  C12N15/55 ,  C12Q1/34 ,  C12R1/19 ,  C12N9/14 ,  C12N9/96

    CPC分类号： C12N9/86

    摘要： The invention concerns a DNA which codes for a protein with N-methylhydantoinase activity and which has1.) the nucleic acid sequence shown in FIG. (1),2.) a sequence corresponding to it within the scope of the degeneracy of the genetic code or(3) a sequence which hybridizes with a sequence from (1) or/and (2) under stringent conditions.Furthermore the invention also concerns a recombinant vector which contains a DNA according to the present invention, a cell which is transformed with a vector according to the present invention as well as a process for producing a recombinant protein with NMHase activity.

    摘要翻译： 本发明涉及编码具有N-甲基乙内酰脲酶活性的蛋白质的DNA，其具有1）。 （1），2.）在遗传密码简并范围内对应的序列或（3）在严格条件下与（1）或/和（2）的序列杂交的序列。 此外，本发明还涉及包含根据本发明的DNA的重组载体，用根据本发明的载体转化的细胞以及用于产生具有NMHase活性的重组蛋白的方法。