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    Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids
    4.
    发明授权
    Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids 有权
    用于制造含有4-羟基酸的聚羟基链烷酸酯聚合物的生物体系

    公开(公告)号：US06316262B1

    公开(公告)日：2001-11-13

    申请号：US09156809

    申请日：1998-09-18

    申请人： Gjalt W. Huisman ,  Frank Skraly ,  David P. Martin ,  Oliver P. Peoples

    发明人： Gjalt W. Huisman ,  Frank Skraly ,  David P. Martin ,  Oliver P. Peoples

    IPC分类号： C12N1554

    CPC分类号： C12N9/0008 ,  C08G63/06 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/8243 ,  C12P7/625

    摘要： The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtaining by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, &agr;-ketoglutarate, or succinate as substrate.

    摘要翻译： 已经从细菌中分离出编码4-羟基丁酰-CoA转移酶的基因，并整合到也表达聚羟基链烷酸酯合酶的细菌的基因组中，以使用包括细菌和植物在内的转基因生物来产生含4HB的聚羟基链烷酸酯的改进的生产方法。 新途径提供了从低廉的碳源（如糖和脂肪酸）生产含有PHB的4HB的方法，其产率高，稳定。 有用的菌株是通过筛选在其基因组中整合了编码4HB-CoA转移酶和/或PHA合成酶的基因的聚合物生产获得的菌株。 用于聚合物生产的方法使用可以利用廉价底物的重组体系。 提供可以利用氨基酸降解途径的系统，α-酮戊二酸或琥珀酸作为底物。

    Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids
    5.
    发明授权
    Biological systems for manufacture of polyhydroxyalkanoate polymers containing 4-hydroxyacids 失效
    用于制造含有4-羟基酸的聚羟基链烷酸酯聚合物的生物体系

    公开(公告)号：US07081357B2

    公开(公告)日：2006-07-25

    申请号：US10773916

    申请日：2004-02-06

    申请人： Gjalt W. Huisman ,  Frank Skraly ,  David P. Martin ,  Oliver P. Peoples

    发明人： Gjalt W. Huisman ,  Frank Skraly ,  David P. Martin ,  Oliver P. Peoples

    IPC分类号： C12P7/62 ,  C12N15/53 ,  C12N15/54

    CPC分类号： C12N9/0008 ,  C08G63/06 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/8243 ,  C12P7/625

    摘要： The gene encoding a 4-hydroxybutyryl-CoA transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtaining by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, α-ketoglutarate, or succinate as substrate.

    摘要翻译： 已经从细菌中分离出编码4-羟基丁酰辅酶A转移酶的基因，并整合到也表达聚羟基链烷酸酯合成酶的细菌的基因组中，以产生使用包括细菌和植物在内的转基因生物体的含4HB的聚羟基链烷酸酯的改进的生产方法。 新途径提供了从低廉的碳源（如糖和脂肪酸）生产含有PHB的4HB的方法，其产率高，稳定。 有用的菌株是通过筛选在其基因组中整合了编码4HB-CoA转移酶和/或PHA合成酶的基因的聚合物生产获得的菌株。 用于聚合物生产的方法使用可以利用廉价底物的重组体系。 提供可以利用氨基酸降解途径的系统，α-酮戊二酸或琥珀酸作为底物。

    Biological Systems for Manufacture of Polyhydroxyalkanoate Polymers Containing 4-Hydroxyacids
    6.
    发明申请
    Biological Systems for Manufacture of Polyhydroxyalkanoate Polymers Containing 4-Hydroxyacids 审中-公开
    用于制造含有4-羟基酸的聚羟基链烷酸酯聚合物的生物系统

    公开(公告)号：US20100093043A1

    公开(公告)日：2010-04-15

    申请号：US11735888

    申请日：2007-04-16

    申请人： Gjalt W. Huisman ,  Frank Skraly ,  David P. Martin ,  Oliver P. Peoples

    发明人： Gjalt W. Huisman ,  Frank Skraly ,  David P. Martin ,  Oliver P. Peoples

    IPC分类号： C12P13/04 ,  C12N1/12 ,  C12N15/74

    CPC分类号： C12N9/0008 ,  C08G63/06 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/8243 ,  C12P7/625

    摘要： The gene encoding a 4-hydroxybutyryl-Co A transferase has been isolated from bacteria and integrated into the genome of bacteria also expressing a polyhydroxyalkanoate synthase, to yield an improved production process for 4HB-containing polyhydroxyalkanoates using transgenic organisms, including both bacteria and plants. The new pathways provide means for producing 4HB containing PHAs from cheap carbon sources such as sugars and fatty acids, in high yields, which are stable. Useful strains are obtaining by screening strains having integrated into their genomes a gene encoding a 4HB-CoA transferase and/or PHA synthase, for polymer production. Processes for polymer production use recombinant systems that can utilize cheap substrates. Systems are provided which can utilize amino acid degradation pathways, α-ketoglutarate, or succinate as substrate.

    摘要翻译： 已经从细菌中分离出编码4-羟基丁酰-CoA转移酶的基因，并整合到也表达聚羟基链烷酸酯合酶的细菌的基因组中，以使用包括细菌和植物在内的转基因生物来产生含4HB的聚羟基链烷酸酯的改进的生产方法。 新途径提供了从低廉的碳源（如糖和脂肪酸）生产含有PHB的4HB的方法，其产率高，稳定。 有用的菌株是通过筛选在其基因组中整合了编码4HB-CoA转移酶和/或PHA合成酶的基因的聚合物生产获得的菌株。 用于聚合物生产的方法使用可以利用廉价底物的重组体系。 提供可以利用氨基酸降解途径的系统，α-酮戊二酸或琥珀酸作为底物。

    Generation of high polyhydroxybutyrate producing oilseeds
    8.
    发明授权
    Generation of high polyhydroxybutyrate producing oilseeds 有权
    生产高聚羟基丁酸酯油籽油

    公开(公告)号：US09181559B2

    公开(公告)日：2015-11-10

    申请号：US13395702

    申请日：2010-09-15

    申请人： Nii Patterson ,  Jihong Tang ,  Jixiang Han ,  Venkata Tavva ,  Andrew Hertig ,  Zhigang Zhang ,  Thomas Martin Ramseier ,  Karen Bohmert-Tatarev ,  Oliver P. Peoples ,  Kristi D. Snell

    发明人： Nii Patterson ,  Jihong Tang ,  Jixiang Han ,  Venkata Tavva ,  Andrew Hertig ,  Zhigang Zhang ,  Thomas Martin Ramseier ,  Karen Bohmert-Tatarev ,  Oliver P. Peoples ,  Kristi D. Snell

    IPC分类号： C12N15/82 ,  C12N15/63 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8214 ,  C12N15/8257

    摘要： Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates (“PHA”) are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate (“PHB”). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

    摘要翻译： 提供了用于合成生物聚合物的转基因植物，植物材料，植物细胞和遗传构建体，例如聚羟基链烷酸酯（“PHA”）。 在一个实施方案中，转基因植物合成聚羟基丁酸酯（“PHB”）。 在一个实施方案中，转基因植物编码编码用于产生PHA的酶的一种或多种基因的siRNA。 在更优选的实施方案中，siRNA表达受诱导型调节元件的控制。 在另一个实施方案中，转基因植物含有编码将降解聚合物的表达酶的转基因。 在优选的实施方案中，这些酶的表达受发芽特异性，诱导型或最小启动子的控制。 在另一个实施方案中，转基因植物含有编码增加聚合物合成碳流的酶的转基因。 在优选的实施方案中，这些转基因编码增加卡尔文循环中碳流的酶。

    Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
    9.
    发明授权
    Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways 有权
    从脂肪酸生物合成途径生产中链长度聚羟基链烷酸酯

    公开(公告)号：US07786355B2

    公开(公告)日：2010-08-31

    申请号：US09991152

    申请日：2001-11-16

    申请人： Stephanie Aquin ,  Oliver P. Peoples ,  Kristi D. Snell

    发明人： Stephanie Aquin ,  Oliver P. Peoples ,  Kristi D. Snell

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N1/20 ,  C12N5/10

    CPC分类号： C12N9/93 ,  C12N9/00 ,  C12N9/10 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/8243

    摘要： Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.

    摘要翻译： 已经开发了使用3-羟基酰基ACP硫酯酶，PHA合成酶和酰基辅酶A合成酶从脂肪酸生物合成途径制备聚羟基链烷酸酯（PHA）的方法。 使用具有3-羟基酰基ACP硫酯酶的催化活性的酶，非天然细菌PHA生产者和植物中的脂肪酸生物合成途径的PHA生产的方法，具有对于中链长度3-羟基脂肪酸具有底物特异性的酰基辅酶A合成酶 ，和中链长度PHA合酶。 酰基辅酶A合成酶活性可以通过宿主生物的内源酰基辅酶A合成酶提供，当充分表达时，或者宿主生物的活性可以通过重组酰基辅酶A合成酶基因的表达来补充。 描述了用于生物量作物的叶绿体，细胞溶质和过氧化物酶体以及油料种子作物的质体，细胞溶质和过氧化物酶体的基于植物的PHA生产的新策略。

    ENZYMES FOR BIOPOLYMER PRODUCTION
    10.
    发明申请
    ENZYMES FOR BIOPOLYMER PRODUCTION 审中-公开
    生物聚合物生产的酶

    公开(公告)号：US20080233629A1

    公开(公告)日：2008-09-25

    申请号：US11926832

    申请日：2007-10-29

    申请人： Oliver P. Peoples ,  Lara L. Madison ,  Gjalt W. Huisman

    发明人： Oliver P. Peoples ,  Lara L. Madison ,  Gjalt W. Huisman

    IPC分类号： C12N15/60 ,  C12N9/88

    CPC分类号： C12N9/0008 ,  C07K2319/00 ,  C12N9/10 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/62 ,  C12N15/82 ,  C12N15/8243 ,  C12P7/625

    摘要： In order to optimize the flux or flow of carbon intermediates from normal cellular metabolism into PHAs it is desirable to optimize the expression of the enzymes of the PHA biosynthetic pathway. Gene fusions are genetic constructs where two open reading frames have been fused into one and encode hybrid proteins and in some cases bifunctional hybrid enzymes. Linkers may be added to spatially separate the two domains of the hybrid protein. In the case of enzymes which catalyse successive reactions in a pathway, the fusion of two genes results in bringing two enzymatic activities into close proximity to each other. When the product of the first reaction is a substrate for the second one, this new configuration of active sites may result in a faster transfer of the product of the first reaction to the second active site with a potential for increasing the flux through the pathway.

    摘要翻译： 为了优化碳中间体从正常细胞代谢到PHAs的通量或流量，优选优化PHA生物合成途径的酶的表达。 基因融合是遗传构建体，其中两个开放阅读框已融合在一起并编码杂交蛋白质，并且在某些情况下编码双功能杂合酶。 可以加入接头以在空间上分离杂交蛋白的两个结构域。 在催化途径中连续反应的酶的情况下，两个基因的融合导致使两个酶活性彼此靠近。 当第一反应的产物是第二反应的底物时，活性位点的这种新构型可以导致第一反应的产物更快地转移到第二活性位点，具有增加通过该通路的通量的可能性。