Starches via modification of expression of starch biosynthetic enzyme genes
    3.
    发明授权
    Starches via modification of expression of starch biosynthetic enzyme genes 失效
    通过修饰淀粉生物合成酶基因表达的淀粉

    公开(公告)号:US06376749B1

    公开(公告)日:2002-04-23

    申请号:US09257894

    申请日:1999-02-25

    IPC分类号: C12N1582

    摘要: The instant invention discloses utilization of a cDNA clone to construct sense and antisense genes for inhibition of starch branching enzyme enzymatic activity in corn. More specifically, this invention concerns a method of controlling the starch fine structure of starch derived from the grain of corn comprising: (1) preparing a chimeric gene comprising a nucleic acid fragment encoding a starch branching enzyme structural gene or a fragment thereof, operably linked in either sense or antisense orientation on the upstream side to a nucleic acid fragment encoding a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a nucleic acid fragment encoding a suitable regulatory sequence for transcriptional termination, and (2) transforming corn with said chimeric gene, wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed corn compared to the fine structure of starch derived from corn not possessing said chimeric gene.

    摘要翻译: 本发明公开了利用cDNA克隆构建用于抑制玉米淀粉分支酶酶活性的有义和反义基因。 更具体地说,本发明涉及一种控制来自玉米粒的淀粉的淀粉精细结构的方法,包括:(1)制备嵌合基因,其包含编码淀粉分支酶结构基因或其片段的核酸片段,其可操作地连接 在上游侧的任何正义或反义方向,编码引导玉米胚乳组织中的基因表达的启动子的核酸片段,并且在下游侧可操作地连接到编码转录终止的合适调控序列的核酸片段,和( 2)用所述嵌合基因转化玉米,其中所述嵌合基因的表达导致衍生自所述转化玉米的淀粉的淀粉的精细结构的改变与源自不具有所述嵌合基因的玉米的淀粉的精细结构相反。

    Using oligonucleotide microarrays to analyze genomic differences for the prediction of heterosis
    4.
    发明授权
    Using oligonucleotide microarrays to analyze genomic differences for the prediction of heterosis 有权
    使用寡核苷酸微阵列分析杂交优势预测的基因组差异

    公开(公告)号:US08996318B2

    公开(公告)日:2015-03-31

    申请号:US12344887

    申请日:2008-12-29

    IPC分类号: G06F19/18 C12Q1/68 A01H1/04

    摘要: A novel method for prediction of the degree of heterotic phenotypes in plants is disclosed. Structural variation analyzes of the genome are used to predict the degree of a heterotic phenotype in plants. In some examples, copy number variation is used to predict the degree of heterotic phenotype. In some methods copy number variation is detected using competitive genomic hybridization arrays. Further, methods for optimizing the arrays are disclosed, together with kits for producing such arrays, as well as hybrid plants selected for development based on the predicted results.

    摘要翻译: 公开了一种预测植物杂种表型程度的新方法。 基因组的结构变异分析用于预测植物中杂种优势表型的程度。 在一些实例中,使用拷贝数变异来预测杂种表型的程度。 在一些方法中,使用竞争性基因组杂交阵列检测拷贝数变异。 此外,公开了用于优化阵列的方法,以及用于生产这种阵列的试剂盒,以及基于预测结果选择用于开发的杂交植物。

    Nucleic acid encoding a wheat brittle-1 homolog
    7.
    发明授权
    Nucleic acid encoding a wheat brittle-1 homolog 有权
    编码小麦脆-1同源物的核酸

    公开(公告)号:US06660850B2

    公开(公告)日:2003-12-09

    申请号:US09796766

    申请日:2001-03-01

    IPC分类号: C12N1529

    CPC分类号: C12N9/1051

    摘要: This invention relates to an isolated nucleic acid fragment encoding a wheat brittle-1-like protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the wheat brittle-1-like protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the wheat brittle-1-like protein in a transformed host cell.

    摘要翻译: 本发明涉及编码小麦脆性-1-样蛋白质的分离的核酸片段。 本发明还涉及在正义或反义方向上构建编码全部或部分小麦脆性-1-样蛋白质的嵌合基因,其中嵌合基因的表达导致小麦脆性-1的水平的改变 在转化的宿主细胞中。

    Using Oligonucleotide Microarrays to Analyze Genomic Differences for the Prediction of Heterosis
    8.
    发明申请
    Using Oligonucleotide Microarrays to Analyze Genomic Differences for the Prediction of Heterosis 有权
    使用寡核苷酸芯片分析基因组差异预测异质性

    公开(公告)号:US20090170712A1

    公开(公告)日:2009-07-02

    申请号:US12344887

    申请日:2008-12-29

    IPC分类号: C40B10/00 C12Q1/68 C40B50/00

    摘要: A novel method for prediction of the degree of heterotic phenotypes in plants is disclosed. Structural variation analyses of the genome are used to predict the degree of a heterotic phenotype in plants. In some examples, copy number variation is used to predict the degree of heterotic phenotype. In some methods copy number variation is detected using competitive genomic hybridization arrays. Further, methods for optimizing the arrays are disclosed, together with kits for producing such arrays, as well as hybrid plants selected for development based on the predicted results.

    摘要翻译: 公开了一种预测植物杂种表型程度的新方法。 基因组的结构变异分析用于预测植物中杂种优势表型的程度。 在一些实例中,使用拷贝数变异来预测杂种表型的程度。 在一些方法中,使用竞争性基因组杂交阵列检测拷贝数变异。 此外,公开了用于优化阵列的方法,以及用于生产这种阵列的试剂盒,以及基于预测结果选择用于开发的杂交植物。