公开(公告)号：US07172682B2

公开(公告)日：2007-02-06

申请号：US10351976

申请日：2003-01-24

申请人： Jonathan S. Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

发明人： Jonathan S. Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

IPC分类号： C07K17/14

CPC分类号： C07K17/14

摘要： Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst-or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.

公开(公告)号：US20070000781A1

公开(公告)日：2007-01-04

申请号：US10351976

申请日：2003-01-24

申请人： Jonathan Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

发明人： Jonathan Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

IPC分类号： C07K1/26 ,  G01N27/00

CPC分类号： C07K17/14

摘要： Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst- or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.

摘要翻译： 本文公开了将生物催化剂固定在微流体生物芯片上以在存在电渗流的情况下进行反应的方法和装置。 生物芯片包括其微流体流动表面上的聚合物，其中聚合物包括选自相同极性的离子基团的第一取代基或其前体，作为疏水基团的第二取代基，和包含固定的生物催化剂或前体的第三取代基 其中。 生物芯片可用于在电渗流的存在下进行多次连续的生物催化反应。

公开(公告)号：US20070108057A1

公开(公告)日：2007-05-17

申请号：US11651269

申请日：2007-01-09

申请人： Jonathan Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

发明人： Jonathan Dordick ,  Moo-Yeal Lee ,  Aravind Srinivasan ,  Bosung Ku

IPC分类号： G01N27/00

CPC分类号： C07K17/14

摘要： Disclosed herein is a method and apparatus of immobilizing a biocatalyst on a microfluidic biochip for conducting reactions in the presence of electroosmotic flow. The biochip includes a polymer on its microfluidic flow surfaces, wherein the polymer includes a first substituent selected from ionic groups of the same polarity or precursors thereof, a second substituent that is a hydrophobic group, and a third substituent comprising an immobilized biocatalyst-or precursor thereof. The biochip can be used to conduct multiple sequential biocatalyzed reactions in the presence of electroosmotic flow.

摘要翻译： 本文公开了将生物催化剂固定在微流体生物芯片上以在存在电渗流的情况下进行反应的方法和装置。 生物芯片包括在其微流体流动表面上的聚合物，其中聚合物包括选自相同极性的离子基团的第一取代基或其前体，作为疏水基团的第二取代基和包含固定的生物催化剂或前体的第三取代基 其中。 生物芯片可用于在电渗流的存在下进行多次连续的生物催化反应。

公开(公告)号：US07427497B2

公开(公告)日：2008-09-23

申请号：US10287440

申请日：2002-11-01

申请人： Jonathan S. Dordick ,  Aravind Srinivasan ,  Jungbae Kim ,  David H. Sherman ,  Douglas S. Clark

发明人： Jonathan S. Dordick ,  Aravind Srinivasan ,  Jungbae Kim ,  David H. Sherman ,  Douglas S. Clark

IPC分类号： C12N11/18 ,  C12N1/00

CPC分类号： B01J19/0093 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00533 ,  B01J2219/00536 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00644 ,  B01J2219/00691 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01J2219/00835 ,  B01J2219/00837 ,  B01J2219/0086 ,  B01L3/5027 ,  B01L3/5085 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2300/0829

摘要： Disclosed herein is a microfluidics device that can be used to prepare natural products and their analogs. The device comprises the enzymes of a biosynthetic pathway immobilized thereon and a means for sequentially directing a starting material and each ensuing reaction product to the enzymes of the biosynthetic pathway in the order corresponding to the steps of the biosynthetic pathway. The device can thus be used to prepare the natural product using the natural starting material of the biosynthetic pathway or analogs of the natural product using an unnatural starting material. Alternatively, artificial pathways can be created by immobilizing an appropriate selection of enzymes on the device in an order whereby each subsequent enzyme can catalyze a reaction with the product of the prior enzyme. Novel chemical entities can be prepared from these artificial pathways.

摘要翻译： 本文公开了可用于制备天然产物及其类似物的微流体装置。 该装置包括固定在其上的生物合成途径的酶，以及以与生物合成途径的步骤相应的顺序将原料和每个随后的反应产物顺序地引导到生物合成途径的酶的装置。 因此，该装置可以用于使用天然起始物质的天然产物或天然产物的类似物使用非天然原料制备天然产物。 或者，可以通过以适当顺序的酶固定适当选择的酶在装置上来产生人工途径，其中每个随后的酶可以催化与先前酶的产物的反应。 这些人造途径可以制备新的化学物质。

公开(公告)号：US07280526B2

公开(公告)日：2007-10-09

申请号：US10053079

申请日：2002-01-17

申请人： Lisa Karen Fleischer ,  Iraj Saniee ,  Frederick Bruce Shepherd ,  Aravind Srinivasan

发明人： Lisa Karen Fleischer ,  Iraj Saniee ,  Frederick Bruce Shepherd ,  Aravind Srinivasan

IPC分类号： H04L12/28 ,  H04J3/16 ,  G01R31/08

CPC分类号： H04L45/50 ,  H04L45/00 ,  H04L45/123 ,  H04L45/28 ,  H04Q11/0062 ,  H04Q2011/0073 ,  H04Q2011/0077 ,  H04Q2011/0081 ,  H04Q2011/0086

摘要： Broadly, techniques for solving network routing within a predetermined error are disclosed. These techniques may be applied to networks supporting dedicated reserve capacity, where reserved capacity on links in the network is dedicated for a particular commodity (generally, a source and sink pair of computers), and shared recovery, where reserved capacity on links is shared amongst two or more commodities. These techniques use an iterative process to determine flows on each of the links in a network. Costs are set for each commodity, and primary and secondary (i.e., backup) flows are initialized. A commodity is selected and demand for the commodity is routed through the shortest path. Costs are updated for each potential failure mode. For each commodity, the flows and costs are updated. Once all flows and costs are updated, then it is determined if a function is less than a predetermined value. If the function is less than a predetermined value, then the commodity selection, and flow and cost adjustments are again performed. If the function is greater than the predetermined amount, then the network routing problem is solved to within a predetermined amount from an optimal network routing.

摘要翻译： 广泛地，公开了在预定误差内解决网络路由的技术。 这些技术可以应用于支持专用储备容量的网络，其中网络上的链路上的保留容量专用于特定商品（通常是源和计算机对），以及共享恢复，其中共享链路上的预留容量 两种以上的商品。 这些技术使用迭代过程来确定网络中每个链路上的流量。 为每个商品设置成本，并初始化主要和次要（即备份）流。 选择商品，并通过最短路径路由商品的需求。 每个潜在的故障模式都会更新成本。 对于每种商品，流量和成本都会更新。 一旦所有流量和成本被更新，则确定功能是否小于预定值。 如果功能小于预定值，则再次执行商品选择，流量和成本调整。 如果该功能大于预定量，则将网络路由问题从最佳网络路由解决到预定量内。

公开(公告)号：US20070226292A1

公开(公告)日：2007-09-27

申请号：US11386610

申请日：2006-03-22

申请人： Madhu Chetuparambil ,  Srinivas Hasti ,  Stephan Hesmer ,  Todd Kaplinger ,  Subbarao Meduri ,  Maxim Moldenhauer ,  Aravind Srinivasan

发明人： Madhu Chetuparambil ,  Srinivas Hasti ,  Stephan Hesmer ,  Todd Kaplinger ,  Subbarao Meduri ,  Maxim Moldenhauer ,  Aravind Srinivasan

IPC分类号： G06F15/16

CPC分类号： G06F9/54

摘要： A Fragment Context Preserver has two components, a Fragmented Request Preserver and a Request Context Helper. The Fragmented Request Preserver program runs on a surrogate attached to a client computer and distributed computer environment, and a Request Context Helper runs on each application server on the distributed computer environment. Working together, these two programs ensure that independently dispatched fragments of a request execute with the required context.

摘要翻译： 片段上下文保存器有两个组件，分片请求保存器和请求上下文帮助器。 分片请求保存程序在连接到客户端计算机和分布式计算机环境的代理上运行，并且请求上下文帮助程序在分布式计算机环境中的每个应用程序服务器上运行。 这两个程序一起工作，确保独立调度的请求的片段与所需的上下文一起执行。

公开(公告)号：US20060127950A1

公开(公告)日：2006-06-15

申请号：US11244826

申请日：2005-10-06

申请人： Carlos Bosques ,  Nishla Keiser ,  Aravind Srinivasan ,  Rahul Raman ,  Karthik Viswanathan ,  Ram Sasisekharan ,  Pankaj Gandhe ,  Sasi Raguram

发明人： Carlos Bosques ,  Nishla Keiser ,  Aravind Srinivasan ,  Rahul Raman ,  Karthik Viswanathan ,  Ram Sasisekharan ,  Pankaj Gandhe ,  Sasi Raguram

IPC分类号： C40B40/10 ,  G01N33/53 ,  C12P21/06

CPC分类号： G01N33/6851 ,  G01N33/66 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2400/10 ,  G16C20/20

摘要： The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins and saccharides attached to lipids. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information from the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation. Such methods are also provided. Methods are further provided to assess production processes, to assess the purity of samples containing glycoconjugates, and to select glycoconjugates with the desired glycosylation.

摘要翻译： 本发明部分涉及碳水化合物的改进分析。 特别地，本发明涉及碳水化合物的分析，例如在蛋白质上发现的N-聚糖和O-聚糖以及与脂质连接的糖。 因此，还提供了改进的方法，用于研究细胞，组织和体液上的糖基化模式。 来自聚糖分析的信息，例如细胞，组织和体液中的糖基化模式，可用于诊断和治疗方法以及促进糖基化/改变糖基化作用的研究。 还提供了这样的方法。 进一步提供方法以评估生产过程，以评估含有糖缀合物的样品的纯度，并选择具有所需糖基化的糖缀合物。

公开(公告)号：US08141042B2

公开(公告)日：2012-03-20

申请号：US11682413

申请日：2007-03-06

申请人： Zhi Gan ,  Ying Chun Guo ,  Rahul Kurane ,  Aravind Srinivasan

发明人： Zhi Gan ,  Ying Chun Guo ,  Rahul Kurane ,  Aravind Srinivasan

IPC分类号： G06F9/44

CPC分类号： G06F8/316 ,  G06F9/445

摘要： A computer-implementable method, system and computer-usable medium for extending the portability of code to a limited-class environment are disclosed. In a preferred embodiment, the method includes the steps of: identifying any full-version-only objects that are not in a reduced-version of a language, wherein the reduced-version of the language comprises a reduced portion of objects that are in a full-version of the language; associating a full-version-only object with a set of one or more objects in the reduced-version of the language, wherein the full-version-only object is functionally equivalent to the set of one or more objects in the reduced-version of the language; identifying any full-version-only objects in a software program; and creating a reduced-version of the software program by replacing any identified full-version-only objects with a functionally equivalent set of one or more objects from the reduced-version of the language.

摘要翻译： 公开了一种用于将代码可移植性扩展到有限级环境的计算机可实现的方法，系统和计算机可用介质。 在优选实施例中，该方法包括以下步骤：识别不在语言的简化版本中的任何完全版本的对象，其中该语言的简化版本包括减少部分的对象 全版本的语言; 将完全版本对象与语言的简化版本中的一个或多个对象的集合相关联，其中，仅全版本对象在功能上等同于缩减版本中的一个或多个对象的集合 语言 识别软件程序中的任何完全版本的对象; 以及通过用语言的简化版本替换具有功能上等价的一个或多个对象的集合的任何已识别的全版本对象来创建软件程序的简化版本。

公开(公告)号：US08000904B2

公开(公告)日：2011-08-16

申请号：US12574161

申请日：2009-10-06

申请人： Nishla Keiser ,  Carlos Bosques ,  Ram Sasisekharan ,  Pankaj Gandhe ,  Sasi Raguram ,  Aravind Srinivasan

发明人： Nishla Keiser ,  Carlos Bosques ,  Ram Sasisekharan ,  Pankaj Gandhe ,  Sasi Raguram ,  Aravind Srinivasan

IPC分类号： G01N31/00

CPC分类号： G01N33/6842 ,  G01N33/6803 ,  G01N33/6851 ,  G01N2400/10 ,  Y10T436/143333

摘要： The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information regarding the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation on protein function. Such methods are also provided. Methods are also provided to assess protein production processes, to assess the purity of proteins produced, and to select proteins with the desired glycosylation.

摘要翻译： 本发明部分涉及碳水化合物的改进分析。 特别地，本发明涉及对蛋白质上发现的碳水化合物如N-聚糖和O-聚糖的分析。 因此，还提供了改进的方法，用于研究细胞，组织和体液上的糖基化模式。 关于聚糖分析的信息，例如细胞，组织和体液中的糖基化模式，可用于诊断和治疗方法以及促进糖基化/改变糖基化对蛋白质功能的影响的研究。 还提供了这样的方法。 还提供了评估蛋白质生产过程，评估产生的蛋白质的纯度和选择具有所需糖基化的蛋白质的方法。

公开(公告)号：US20100144553A1

公开(公告)日：2010-06-10

申请号：US12623070

申请日：2009-11-20

申请人： Carlos Bosques ,  Nishla Keiser ,  Aravind Srinivasan ,  Rahul Raman ,  Karthik Viswanathan ,  Ram Sasisekharan ,  Pankaj Gandhe ,  Sasi Raguram

发明人： Carlos Bosques ,  Nishla Keiser ,  Aravind Srinivasan ,  Rahul Raman ,  Karthik Viswanathan ,  Ram Sasisekharan ,  Pankaj Gandhe ,  Sasi Raguram

IPC分类号： C40B40/12

CPC分类号： G01N33/6851 ,  G01N33/66 ,  G01N33/68 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2400/10 ,  G06F19/703

摘要： The invention relates, in part, to the improved analysis of carbohydrates. In particular, the invention relates to the analysis of carbohydrates, such as N-glycans and O-glycans found on proteins and saccharides attached to lipids. Improved methods, therefore, for the study of glycosylation patterns on cells, tissue and body fluids are also provided. Information from the analysis of glycans, such as the glycosylation patterns on cells, tissues and in body fluids, can be used in diagnostic and treatment methods as well as for facilitating the study of the effects of glycosylation/altered glycosylation. Such methods are also provided. Methods are further provided to assess production processes, to assess the purity of samples containing glycoconjugates, and to select glycoconjugates with the desired glycosylation.

摘要翻译： 本发明部分涉及碳水化合物的改进分析。 特别地，本发明涉及碳水化合物的分析，例如在蛋白质上发现的N-聚糖和O-聚糖以及与脂质连接的糖。 因此，还提供了改进的方法，用于研究细胞，组织和体液上的糖基化模式。 来自聚糖分析的信息，例如细胞，组织和体液中的糖基化模式，可用于诊断和治疗方法以及促进糖基化/改变糖基化作用的研究。 还提供了这样的方法。 进一步提供方法以评估生产过程，以评估含有糖缀合物的样品的纯度，并选择具有所需糖基化的糖缀合物。