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公开(公告)号：US07964380B2
公开(公告)日：2011-06-21
申请号：US11338124
申请日：2006-01-23
CPC分类号： G01N33/54346 , B01D15/3828 , B01J20/289 , B01J20/3204 , B01J20/3272 , B01J20/3274 , B01J20/3289 , B82Y5/00 , B82Y15/00 , B82Y30/00 , G01N2800/52
摘要： Matrices for manipulation of biopolymers, including the separation, purification, immobilization and archival storage of biopolymers is disclosed.
摘要翻译： 公开了用于操作生物聚合物的基质，包括生物聚合物的分离，纯化，固定和存档。
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公开(公告)号：US20090182120A1
公开(公告)日：2009-07-16
申请号：US12054325
申请日：2008-03-24
CPC分类号： G01N33/54346 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C12N15/1013 , G01N33/54326 , Y10T436/25375
摘要： Materials and methods for surface mediated self assembly of nanoparticles for the isolation of biomolecules is provided.
摘要翻译： 提供了用于分离生物分子的纳米颗粒的表面介导的自组装的材料和方法。
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公开(公告)号：US08288169B2
公开(公告)日：2012-10-16
申请号：US12054325
申请日：2008-03-24
CPC分类号： G01N33/54346 , B82Y5/00 , B82Y15/00 , B82Y30/00 , C12N15/1013 , G01N33/54326 , Y10T436/25375
摘要： Materials and methods for surface mediated self-assembly of nanoparticles for the isolation of biomolecules is provided.
摘要翻译： 提供了用于分离生物分子的纳米颗粒的表面介导的自组装的材料和方法。
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公开(公告)号：US20090208919A1
公开(公告)日：2009-08-20
申请号：US12015402
申请日：2008-01-16
CPC分类号： C07H21/00 , B82Y5/00 , B82Y15/00 , C07K1/32 , C12N5/0634 , C12N11/14 , C12N15/10 , G01N33/54346
摘要： Matrices for manipulation of biopolymers, including the separation, purification, immobilization and archival storage of biopolymers is disclosed.
摘要翻译： 公开了用于操作生物聚合物的基质，包括生物聚合物的分离，纯化，固定和存档。
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公开(公告)号：US10272409B2
公开(公告)日：2019-04-30
申请号：US12080720
申请日：2008-04-04
申请人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
发明人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
摘要： Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.
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公开(公告)号：US20130101981A1
公开(公告)日：2013-04-25
申请号：US13304298
申请日：2011-11-23
CPC分类号： C07H21/00 , B82Y5/00 , B82Y15/00 , C07K1/32 , C12N5/0634 , C12N11/14 , C12N15/10 , G01N33/54346
摘要： Matrices for manipulation of biopolymers, including the separation, purification, immobilization and archival storage of biopolymers is disclosed.
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7.发明申请Methods and devices based upon a novel form of nucleic acid duplex on a surface 审中-公开基于表面上核酸双链体的新形式的方法和装置公开(公告)号：US20090011949A1
公开(公告)日：2009-01-08
申请号：US12080720
申请日：2008-04-04
申请人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
发明人： Michael E. Hogan , Sergy Lemeshko , Yuri Belosludtsev , Thomas F. Powdrill , Rahul Mitra , Joseph G. Utermohlen , Frederick H. Eggers
CPC分类号： B01J19/0046 , B01J2219/00376 , B01J2219/00497 , B01J2219/005 , B01J2219/00576 , B01J2219/00605 , B01J2219/00653 , B01J2219/00659 , B01J2219/00677 , B01J2219/00691 , B01J2219/00722
摘要： Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.
摘要翻译： 本文提供的生物分子杂交装置包括具有永久且共价连接的官能团表面的底物和未修饰的单链寡核苷酸的吸附单层，其长度为10至约24个碱基，作为约束寡核苷酸的饱和膜 通过每个寡核苷酸的基本上所有磷酸基团的直接非共价磷酸盐表面吸附接触表面。 受约束的寡核苷酸有效地与具有不对称，非螺旋碱基配对并且没有寡核苷酸与装置表面解离的互补单链核酸解离地杂交。 此外，提供了用于将溶液状态靶核酸杂交以探测核酸并用于鉴定核苷酸结合蛋白使用生物分子杂交装置结合的核苷酸序列的方法。
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公开(公告)号：US20220298547A1
公开(公告)日：2022-09-22
申请号：US17698400
申请日：2022-03-18
申请人： Michael E. Hogan
发明人： Michael E. Hogan
IPC分类号： C12Q1/686 , C12Q1/70 , C12Q1/689 , C12Q1/6853 , C12Q1/6874
摘要： Provided herein are methods for isolating, detecting and analyzing a virus in a sample. Generally, the virus is isolated via centrifuging, incubating, aggregating, and lysing the sample to obtain a crude neutralized lysate. The crude neutralized lysate is analyzed and the virus(es) are detected by performing one of a first polymerase chain reaction (PCR) amplification followed by a second PCR amplification, by an isothermal amplification or by the reverse transcription reaction and the first polymerase chain reaction amplification together utilizing fluorescently labeled primer pairs and hybridizing the resultant fluorescent labeled amplicons to complementary nucleic acid probes.
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公开(公告)号：US20210130898A1
公开(公告)日：2021-05-06
申请号：US17087099
申请日：2020-11-02
IPC分类号： C12Q1/6883 , G01N33/04 , C12N1/20 , C12N1/14
摘要： Provided herein is a method for identifying a mastitis-causing microbe in a subject. A milk sample is centrifuged to form a microbial pellet, total nucleic acids are extracted from the pellet and a microarray analysis of extracted DNA from which the mastitis-causing microbe is identified from DNA hybridization to mastitis-causing microbe species-specific gene probes. Also provided is a method for diagnosing a bovine mastitis infection in a dairy cow after identifying the bovine mastitis-causing microbe in a raw milk sample from the dairy cow.
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公开(公告)号：US07667026B2
公开(公告)日：2010-02-23
申请号：US11711561
申请日：2007-02-27
CPC分类号： C12Q1/6837 , C12Q1/6881 , C12Q2600/156
摘要： The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.
摘要翻译： 本发明提供了一种用于现场环境中实时种群规模HLA基因分型和/或等位基因的便携式系统以及这种群体规模HLA基因分型的方法。 系统的各个组件可以在现场环境中移植到并可操作，从而通过实时基因或等位基因提供高吞吐量。 还提供了HLA基因特异性引物和HLA等位基因特异性或单核苷酸多态性特异性杂交探针。 此外，本发明提供了包含杂交探针的微阵列。 还提供了包含HLA基因特异性引物和微阵列的试剂盒。
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