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    Method and apparatus for measurement of binding between a protein and a nucleotide
    1.
    发明申请
    Method and apparatus for measurement of binding between a protein and a nucleotide 审中-公开
    用于测量蛋白质和核苷酸之间结合的方法和装置

    公开(公告)号：US20070014692A1

    公开(公告)日：2007-01-18

    申请号：US11495047

    申请日：2006-07-28

    申请人： Judith Erb ,  James Downward ,  Otho Ulrich

    发明人： Judith Erb ,  James Downward ,  Otho Ulrich

    IPC分类号： G01N21/64

    CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/7703 ,  G01N2021/6484 ,  G01N2021/7786 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/814 ,  Y10S436/817 ,  Y10S436/818 ,  Y10S977/924 ,  Y10S977/958 ,  Y10S977/959

    摘要： A method and apparatus for measuring binding between a plurality of molecules of a first type and a plurality of molecules of a second type is presented. Apparatus utilizes a sensor possessing a waveguide to which have been attached in close proximity to its surface, features resembling molecules of said first type. Light is injected into said waveguide so as to produce an evanescent field at its surface. Molecules of said second type are tagged with a tag belonging to that class of chemicals which alters a characteristic of light, when said light passes through said chemical tag. Apparatus utilizes a rapid means of monitoring the change in optical signal coming from said waveguide as binding proceeds between tagged molecules of type 2 and the feature resembling molecules of type 1 on said waveguide. This allows direct measurement of binding and dissociation rates between the two types of molecules. Methods are provided whereby such data may be used to compute affinity constants, binding activity, complex affinity constants resulting from cooperativity, and kinetic parameters for the molecular pair. Preferred embodiments of the invention illustrate application of the method and apparatus to measuring binding between biological receptors and their nuclear response elements, and the use of this type of measurement for assessment of the activity of hormonal mimics present in a sample, for evaluation of pharmaceuticals intended to treat hormone dependent cancers, and for evaluation of tissue biopsy samples to detect mutant forms of the p53 protein.

    摘要翻译： 提出了用于测量第一类型的多个分子与第二类型的多个分子之间的结合的方法和装置。 装置利用具有波导的传感器，其已经附着在其表面附近，特征类似于所述第一类型的分子。 光注入到所述波导中，以便在其表面产生渐逝场。 当所述光通过所述化学标签时，所述第二类型的分子用属于所述类别的化学品的标签标记，所述标签改变光的特性。 装置利用快速的方式监测来自所述波导的光信号的变化，因为在所述波导上标记的类型2的分子和类似于类型1的分子的特征之间的结合进行。 这允许直接测量两种类型的分子之间的结合和解离速率。 提供了这样的方法，其中这样的数据可以用于计算亲和常数，结合活性，由协同性产生的复合亲和常数和分子对的动力学参数。 本发明的优选实施方案说明了用于测量生物受体及其核应答元件之间的结合的方法和装置的应用，以及使用这种类型的测量来评估样品中存在的激素模拟物的活性，以评估药物的目的 治疗激素依赖性癌症，以及用于评估组织活检样品以检测p53蛋白的突变体形式。

    Fiber-optic sensor array
    2.
    发明申请
    Fiber-optic sensor array 审中-公开
    光纤传感器阵列

    公开(公告)号：US20050181432A1

    公开(公告)日：2005-08-18

    申请号：US11095971

    申请日：2005-03-30

    申请人： Eric Priuska ,  Richard Smith ,  Judith Erb ,  James Downward ,  Otho Ulrich

    发明人： Eric Priuska ,  Richard Smith ,  Judith Erb ,  James Downward ,  Otho Ulrich

    IPC分类号： G01N21/25 ,  G01N21/64 ,  G01N33/533 ,  G01N33/543 ,  C12Q1/68 ,  G01N33/53

    CPC分类号： G01N21/648 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6458 ,  G01N33/533 ,  G01N33/54306 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6482

    摘要： A method for performing a rapid, homogenous assays for monitoring the reactions of a binding target, by immobilizing a fluorescent-capable chelate complex that is derivatized so as to posses recognition binding ligands, labeling the complex with a labeled second chelator that is added to the assay thereby forming a fluorescent mixed chelate, and measuring the fluorescent mixed chelate, whereby the measurement of the label enable monitoring of the reaction of the binding target. A rapid assay for performing the above method including a first chelating molecule, a fluorescent-capable ion complexed with the first chelating molecule, a second chelating molecule for reacting with the fluorescent-capable ion complexed with the first chelating molecule, and a measuring device for measuring fluorescent resulting from the second chelating molecule reacting with the fluorescent-capable ion complexed with the first chelating molecule. A biosensor for monitoring molecular interactions between receptors, including a biosensor having attached thereto a fluorescent-capable ion complexed with a first chelating molecule, whereby upon exposure to a second chelating molecule said complex becomes fluorescent is also provided.

    摘要翻译： 一种用于进行用于监测结合靶的反应的快速均质测定的方法，通过固定衍生化以具有识别结合配体的荧光能螯合物复合物，将标记的第二螯合剂标记在添加到 测定荧光混合螯合物，测定荧光混合螯合物，由此标记物的测定能够监测结合靶的反应。 一种用于执行上述方法的快速测定法，包括第一螯合分子，与第一螯合分子络合的荧光能离子，用于与与第一螯合分子络合的荧光能离子反应的第二螯合分子，以及用于 测量由第二螯合分子产生的荧光与与第一螯合分子络合的具有荧光能力的离子反应。 用于监测受体之间的分子相互作用的生物传感器，包括附着有与第一螯合分子络合的荧光能离子的生物传感器，由此当暴露于第二螯合分子时，还提供所述络合物变为荧光的生物传感器。

    Method and apparatus for measurement of binding between a protein and a nucleotide
    5.
    发明授权
    Method and apparatus for measurement of binding between a protein and a nucleotide 失效
    用于测量蛋白质和核苷酸之间结合的方法和装置

    公开(公告)号：US06251688B1

    公开(公告)日：2001-06-26

    申请号：US09045223

    申请日：1998-03-20

    申请人： Judith L. Erb ,  James G. Downward ,  John R. Erb-Downward ,  Otho Ulrich

    发明人： Judith L. Erb ,  James G. Downward ,  John R. Erb-Downward ,  Otho Ulrich

    IPC分类号： G01N33543

    CPC分类号： G01N21/648 ,  G01N21/552 ,  G01N21/6428 ,  G01N21/7703 ,  G01N2021/6484 ,  G01N2021/7786 ,  Y10S435/808 ,  Y10S436/805 ,  Y10S436/814 ,  Y10S436/817 ,  Y10S436/818 ,  Y10S977/924 ,  Y10S977/958 ,  Y10S977/959

    摘要： A method and apparatus for measuring binding between a plurality of molecules of a first type and a plurality of molecules of a second type is presented. Apparatus utilizes a sensor possessing a waveguide to which have been attached in close proximity to its surface, features resembling molecules of said first type. Light is injected into said waveguide so as to produce an evanescent field at its surface. Molecules of said second type are tagged with a tag belonging to that class of chemicals which alters a characteristic of light, when said light passes through said chemical tag. Apparatus utilizes a rapid means of monitoring the change in optical signal coming from said waveguide as binding proceeds between tagged molecules of type 2 and the feature resembling molecules of type 1 on said waveguide. This allows direct measurement of binding and dissociation rates between the two types of molecules. Methods are provided whereby such data may be used to compute affinity constants, binding activity, complex affinity constants resulting from cooperativity, and kinetic parameters for the molecular pair. Preferred embodiments of the invention illustrate application of the method and apparatus to measuring binding between biological receptors and their nuclear response elements, and the use of this type of measurement for assessment of the activity of hormonal mimics present in a sample, for evaluation of pharmaceuticals intended to treat hormone dependent cancers, and for evaluation of tissue biopsy samples to detect mutant forms of the p53 protein.

    摘要翻译： 提出了用于测量第一类型的多个分子与第二类型的多个分子之间的结合的方法和装置。 装置利用具有波导的传感器，其已经附着在其表面附近，特征类似于所述第一类型的分子。 光注入到所述波导中，以便在其表面产生渐逝场。 当所述光通过所述化学标签时，所述第二类型的分子用属于所述类别的化学品的标签标记，所述标签改变光的特性。 装置利用快速的方式监测来自所述波导的光信号的变化，因为在所述波导上标记的类型2的分子和类似于类型1的分子的特征之间的结合进行。 这允许直接测量两种类型的分子之间的结合和解离速率。 提供了这样的方法，其中这样的数据可以用于计算亲和常数，结合活性，由协同性产生的复合亲和常数和分子对的动力学参数。 本发明的优选实施方案说明了用于测量生物受体及其核应答元件之间的结合的方法和装置的应用，以及使用这种类型的测量来评估样品中存在的激素模拟物的活性，以评估药物的目的 治疗激素依赖性癌症，以及用于评估组织活检样品以检测p53蛋白的突变体形式。