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    Mold capable of degrading dioxin, degradation of dioxin with the use of the same, method for producing composts capable of degrading dioxin and method for growing plants
    1.
    发明授权
    Mold capable of degrading dioxin, degradation of dioxin with the use of the same, method for producing composts capable of degrading dioxin and method for growing plants 失效
    能够降解二恶英的模具，二恶英的降解，使用它们的二恶英的降解方法以及能够降解二恶英的堆肥的生产方法和生长植物的方法

    公开(公告)号：US06506956B1

    公开(公告)日：2003-01-14

    申请号：US09674278

    申请日：2000-10-30

    申请人： Junichiro Yuki ,  Motoshi Suzuki ,  Hideo Miyamoto

    发明人： Junichiro Yuki ,  Motoshi Suzuki ,  Hideo Miyamoto

    IPC分类号： A62D300

    CPC分类号： C12R1/645 ,  B09C1/105 ,  C05F11/08 ,  C05F17/0036 ,  C05F17/009 ,  C12N1/14 ,  C12N1/22 ,  C12R1/80 ,  Y02P20/145 ,  Y02W30/43

    摘要： The invention provides lignin-degradable fungi capable of degrading dioxins that have accumulated in the soil for farm crops, into harmless substances; a method for degrading dioxins by applying the fungi to a dioxin-containing object to such a degree that the cell concentration in the object reaches at least 1×102 cfu per gram of the object; compost containing the fungi; a method for producing compost that comprises a step of thermally fermenting a lignin-containing, plant-derived organic material to be compost, at a temperature falling between 65 and 100° C. for at least 2 hours, followed by inoculating the thus-fermented material with lignin degrading enzymes-producing fungi and further fermenting it; and a method of using the compost for cultivating plants.

    摘要翻译： 本发明提供了能够降解积累在农作物土壤中的二恶英的木质素可降解真菌成为无害物质; 通过将真菌施用于含二恶英的物体来降解二恶英的方法，使得物体中的细胞浓度达到每克物体至少1×102cfu的程度; 含有真菌的堆肥 一种生产堆肥的方法，其包括在温度为65-100℃的温度下将含木质素的植物衍生的有机材料发酵成堆肥的步骤至少2小时，然后将发酵的 具有木质素降解酶生产真菌并进一步发酵的材料; 以及使用堆肥来培育植物的方法。

    THERMOSTABLE POLYMERASES HAVING ALTERED FIDELITY AND METHODS OF IDENTIFYING AND USING SAME
    4.
    发明申请
    THERMOSTABLE POLYMERASES HAVING ALTERED FIDELITY AND METHODS OF IDENTIFYING AND USING SAME 审中-公开
    具有变化的可塑性聚合物及其识别方法和使用方法

    公开(公告)号：US20090004656A1

    公开(公告)日：2009-01-01

    申请号：US11962067

    申请日：2007-12-20

    申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    IPC分类号： C12Q1/68 ,  C12Q1/48 ,  C12N9/10 ,  C12N15/11 ,  C12P19/34

    CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

    摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

    摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。

    File management apparatus and method
    5.
    发明授权
    File management apparatus and method 失效
    文件管理装置和方法

    公开(公告)号：US07020664B1

    公开(公告)日：2006-03-28

    申请号：US09723972

    申请日：2000-11-28

    申请人： Tomoko Yamaguchi ,  Toshio Mizuno ,  Motoshi Suzuki

    发明人： Tomoko Yamaguchi ,  Toshio Mizuno ,  Motoshi Suzuki

    IPC分类号： G06F12/00

    CPC分类号： G06F17/30067

    摘要： Upon receipt of a segment name obtainment request, the segment name generation unit 104 generates a segment name composed of (1) a file name of a file to which the segment belongs and (2) a number indicating a serial number of the segment in the file. Upon receipt of an access request specifying a segment name, the segment identification unit 119 refers to the segment name to detect the file name and the serial number of the segment in the detected file. The segment position detection unit 106 then refers to the file information storage unit 102 and the segment information storage unit 103 to detect a position where a segment is to be accessed.

    摘要翻译： 在接收到段名称获取请求时，段名称生成单元104生成由（1）段所属的文件的文件名构成的段名，（2）表示段的名称的序号， 文件。 在接收到指定段名称的访问请求时，段识别单元119参考段名来检测检测文件中的段的文件名和序列号。 然后，段位置检测单元106参考文件信息存储单元102和段信息存储单元103以检测段被访问的位置。

    Vacuum booster apparatus and a brake apparatus
    6.
    发明授权
    Vacuum booster apparatus and a brake apparatus 有权

    公开(公告)号：US06398316B1

    公开(公告)日：2002-06-04

    申请号：US09285052

    申请日：1999-04-02

    申请人： Yasuji Mizutani ,  Motoshi Suzuki ,  Hirofumi Nitta

    发明人： Yasuji Mizutani ,  Motoshi Suzuki ,  Hirofumi Nitta

    IPC分类号： B60T844

    CPC分类号： F02D9/02 ,  B60T8/4809 ,  B60T13/683 ,  B60T13/686 ,  B60T13/72

    摘要： A vacuum booster apparatus used for a brake apparatus positively decreases a degree of opening of a throttle valve when it is required while an unnecessary decrease in the degree of opening of the throttle valve is positively prevented. A first pressure chamber of a vacuum booster is connected to an intake pipe of the engine on a downstream side of a throttle valve. A second pressure chamber of the vacuum booster is selectively connectable to one of the first pressure chamber and atmosphere. The vacuum booster boosts an operating force applied to a brake operating member based on a pressure difference between the first pressure chamber and the second pressure chamber. A throttle-opening-degree control device controls a negative pressure in the first pressure chamber by controlling the degree of opening of the throttle valve. The throttle-opening-degree control device decreases the degree of opening of the throttle valve only for a limited time when the negative pressure in the first pressure chamber is smaller than a predetermined necessary negative pressure determined by an operating-force relating amount related to the operating force applied to the brake operating member.

    Thermostable polymerases having altered fidelity and method of identifying and using same
    7.
    发明授权
    Thermostable polymerases having altered fidelity and method of identifying and using same 失效
    具有改变的保真度的热稳定性聚合酶和鉴定和使用它们的方法

    公开(公告)号：US06395524B2

    公开(公告)日：2002-05-28

    申请号：US08978806

    申请日：1997-11-26

    申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    IPC分类号： C12N910

    CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

    摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

    摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。

    Machine tool having parallel structure
    8.
    发明授权
    Machine tool having parallel structure 失效
    机床具有平行结构

    公开(公告)号：US5941128A

    公开(公告)日：1999-08-24

    申请号：US950438

    申请日：1997-10-15

    申请人： Taizo Toyama ,  Motoshi Suzuki

    发明人： Taizo Toyama ,  Motoshi Suzuki

    IPC分类号： B23Q1/00 ,  B23Q1/44 ,  B25J9/04 ,  B25J11/00 ,  B25J17/00 ,  B25J17/02 ,  B25J18/00 ,  F16B7/10

    CPC分类号： B25J17/0266 ,  B25J17/0208 ,  Y10T403/32008 ,  Y10T409/308232 ,  Y10T74/20335

    摘要： A machine tool includes a base, a traveling plate to which a tool is to be attached, plural rods, and plural drive mechanisms fixed to the base. Each of the drive mechanisms is provided with a slide table movable on the drive mechanism and with an actuator for moving the slide table. One end of each of the rods is swingably connected to the corresponding slide tables through a first joint and the other end of each of the rods is swingably connected to the traveling plate through a second joint. Each of the first and the second joints include two rolling elements, each of which is rotatably supported by a rolling bearing to swing the rod.As the rolling elements are supported by the rolling bearings without a clearance, the positioning accuracy of the machine tool is enhanced.

    摘要翻译： 机床包括基座，待安装工具的行进板，多个杆和固定到基座的多个驱动机构。 每个驱动机构设置有可在驱动机构上移动的滑动台和用于移动滑动台的致动器。 每个杆的一端通过第一接头可摆动地连接到相应的滑动台，并且每个杆的另一端通过第二接头可摆动地连接到行进板。 第一和第二关节中的每一个包括两个滚动元件，每个滚动元件由滚动轴承可旋转地支撑以摆动杆。 由于滚动体由滚动轴承支撑而没有间隙，因此机床的定位精度得以提高。

    Brake Control Apparatus for Vehicle
    9.
    发明申请
    Brake Control Apparatus for Vehicle 有权
    车辆制动控制装置

    公开(公告)号：US20080255744A1

    公开(公告)日：2008-10-16

    申请号：US12100217

    申请日：2008-04-09

    申请人： Yoshiyuki Yasui ,  Hiroaki Aizawa ,  Motoshi Suzuki

    发明人： Yoshiyuki Yasui ,  Hiroaki Aizawa ,  Motoshi Suzuki

    IPC分类号： G06F19/00

    CPC分类号： B60T13/662 ,  B60T8/1755 ,  B60T8/17616 ,  B60T8/1766 ,  B60T13/686

    摘要： A brake control apparatus for a vehicle includes four wheel braking apparatuses, a first hydraulic pressure generating apparatus, a front-wheel hydraulic circuit connecting the hydraulic pressure generating apparatus to the two of the wheel braking apparatuses, a rear-wheel hydraulic circuit connecting the hydraulic pressure generating apparatus to the other two of the wheel braking apparatuses, a second hydraulic pressure generating apparatus generating an auxiliary pressure, a braking operation variable detecting means, a reference amount determining means for determining a reference amount of auxiliary hydraulic pressure, an obtaining means for obtaining at least one of state quantities indicating a load condition, a driving condition and slipperiness of wheel, a target amount determining means determining a target amount of auxiliary hydraulic pressure of each hydraulic circuit to be equal to or greater than the corresponding reference amount of auxiliary hydraulic pressure, and a pressure regulating means for regulating the auxiliary hydraulic pressure.

    摘要翻译： 车辆的制动控制装置包括四轮制动装置，第一液压发生装置，将液压发生装置连接到两个车轮制动装置的前轮液压回路，连接液压的后轮液压回路 另外两个车轮制动装置的压力发生装置，产生辅助压力的第二液压发生装置，制动操作变量检测装置，用于确定辅助液压参考量的基准量确定装置，用于 获得指示负载状态，车辆的行驶状况和滑动性的状态量中的至少一个，将每个液压回路的辅助液压的目标量确定为相应的辅助参考量的目标量确定装置 液压，和a 用于调节辅助液压的压力调节装置。

    Thermostable polymerases having altered fidelity and methods of identifying and using same
    10.
    发明授权
    Thermostable polymerases having altered fidelity and methods of identifying and using same 有权
    具有改变的保真度的热稳定性聚合酶以及鉴定和使用它们的方法

    公开(公告)号：US07312059B2

    公开(公告)日：2007-12-25

    申请号：US11096645

    申请日：2005-03-31

    申请人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    发明人： Lawrence A. Loeb ,  Leroy Hood ,  Motoshi Suzuki

    IPC分类号： C12N9/10 ,  C07K1/00

    CPC分类号： C12N9/1252 ,  C12Q1/6858 ,  C12Q1/6883

    摘要： The present invention provides a method for identifying a thermostable polymerase having altered fidelity. The method consists of generating a random population of polymerase mutants by mutating at least one amino acid residue of a thermostable polymerase and screening the population for one or more active polymerase mutants by genetic selection. For example, the invention provides a method for identifying a thermostable polymerase having altered fidelity by mutating at least one amino acid residue in an active site O-helix of a thermostable polymerase. The invention also provides thermostable polymerases and nucleic acids encoding thermostable polymerases having altered fidelity, for example, high fidelity polymerases and low fidelity polymerases. The invention additionally provides a method for identifying one or more mutations in a gene by amplifying the gene with a high fidelity polymerase. The invention further provides a method for accurately copying repetitive nucleotide sequences using a high fidelity polymerase mutant. The invention also provides a method for diagnosing a genetic disease using a high fidelity polymerase mutant. The invention further provides a method for randomly mutagenizing a gene by amplifying the gene using a low fidelity polymerase mutant.

    摘要翻译： 本发明提供了鉴定具有改变的保真度的热稳定聚合酶的方法。 该方法包括通过突变热稳定聚合酶的至少一个氨基酸残基并通过遗传选择筛选一个或多个活性聚合酶突变体的群体来产生聚合酶突变体的随机群体。 例如，本发明提供了通过突变热稳定聚合酶的活性位点O-螺旋中的至少一个氨基酸残基来鉴定具有改变的保真度的热稳定聚合酶的方法。 本发明还提供了具有改变的保真度的热稳定聚合酶和编码耐热聚合酶的核酸，例如高保真聚合酶和低保真度聚合酶。 本发明另外提供了通过用高保真聚合酶扩增基因来鉴定基因中的一个或多个突变的方法。 本发明还提供了使用高保真聚合酶突变体准确地复制重复核苷酸序列的方法。 本发明还提供了使用高保真聚合酶突变体来诊断遗传疾病的方法。 本发明还提供了使用低保真度聚合酶突变体扩增基因来随机诱变基因的方法。