公开(公告)号：US20240018562A1

公开(公告)日：2024-01-18

申请号：US18452884

申请日：2023-08-21

Applicant: KANEKA CORPORATION

Inventor： Rina Aoki ,  Shingo Kobayashi ,  Naoaki Taoka

IPC: C12P21/02 ,  C12N9/02 ,  C12N9/04

CPC classification number: C12P21/02 ,  C12N9/0008 ,  C12Y102/01008 ,  C12N9/0006 ,  C12Y101/01001

Abstract: A cyclic lipopeptide-producing microbial strain improve productivity of a cyclic lipopeptide, such as surfactin, via microbial culture. The cyclic lipopeptide-producing microbial strain, lack at least one of a gene encoding betaine aldehyde dehydrogenase (EC:1.2.1.8) or a gene encoding choline dehydrogenase (EC:1.1.1.1), and a method for producing a cyclic lipopeptide comprising culturing such microbial strain are provided.

公开(公告)号：US11268113B2

公开(公告)日：2022-03-08

申请号：US16616276

申请日：2018-05-23

Applicant: KANEKA CORPORATION

Inventor： Rina Aoki ,  Shunsuke Sato

IPC: C12N1/20 ,  C12P7/62 ,  C12N9/10 ,  C12N15/74 ,  C12P7/625

Abstract: Provided are a PHA-producing microorganism producing a higher molecular weight PHA and a PHA production method using the PHA-producing microorganism. A PHA-producing microorganism including a gene encoding a PHA synthase derived from genus Aeromonas, in which at least a portion of a PHA degrading enzyme gene is altered by substitution, deletion, insertion, and/or addition to reduce or eliminate activity of a PHA degrading enzyme encoded by the gene, and further a glycerol kinase activity is enhanced.

公开(公告)号：US11225676B2

公开(公告)日：2022-01-18

申请号：US16670042

申请日：2019-10-31

Applicant: KANEKA CORPORATION

Inventor： Shingo Kobayashi ,  Rina Aoki ,  Tetsuya Minami ,  Hisashi Arikawa ,  Keiji Matsumoto

IPC: C12P7/62 ,  C12N9/10 ,  C08G63/06 ,  C12N9/88 ,  C12N15/09 ,  C08L101/16

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.

公开(公告)号：US10519473B2

公开(公告)日：2019-12-31

申请号：US15129726

申请日：2015-03-27

Applicant: KANEKA CORPORATION

Inventor： Shingo Kobayashi ,  Rina Aoki ,  Tetsuya Minami ,  Hisashi Arikawa ,  Keiji Matsumoto

IPC: C12P7/62 ,  C12N9/10 ,  C12N9/88

Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.