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公开(公告)号：US11000035B2
公开(公告)日：2021-05-11
申请号：US15658799
申请日：2017-07-25
Applicant: NATIONAL RESEARCH AND DEVELOPMENT AGENCY NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION , KANEKA CORPORATION
Inventor： Hitoshi Nakamura , Koichi Suzaki , Takahiko Sugaya , Shingo Kobayashi
Abstract: Disclosed herein is a soil-borne disease control method capable of simply suppressing a soil-borne pathogen. The method for controlling a soil-borne disease includes applying at least one polyhydroxyalkanoic acid having a structure represented by the following formula (1) to soil: [—CHR—CH2—CO—O—] (1) (wherein R is an alkyl group represented by CnH2n+1 and n is an integer of 1 or more but 15 or less).
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公开(公告)号：US11186831B2
公开(公告)日：2021-11-30
申请号：US16962282
申请日：2019-01-10
Applicant: KANEKA CORPORATION
Inventor： Shingo Kobayashi , Shinichi Yoshida , Shunsuke Sato , Naoaki Taoka
Abstract: Provided is a mutant PHA synthase which produces of a PHA copolymer with a high or low 3HH ratio while maintaining PHA productivity. The mutant PHA synthase is a mutant polyhydroxyalkanoate synthase having an amino acid sequence having 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and having at least one of the following mutations (a) to (c): (a) a substitution of serine at 389th position from N-terminus of the amino acid sequence of SEQ ID NO: 1 with an amino acid other than serine; (b) a substitution of leucine at 436th position from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with an amino acid other than leucine; and (c) a deletion of 11 to 19 amino acid residues from the C-terminus of the amino acid sequence of SEQ ID NO: 1.
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Patent Agency Ranking
3.发明公开公开(公告)号：US20240018562A1
公开(公告)日：2024-01-18
申请号：US18452884
申请日：2023-08-21
Applicant: KANEKA CORPORATION
Inventor： Rina Aoki , Shingo Kobayashi , Naoaki Taoka
CPC classification number: C12P21/02 , C12N9/0008 , C12Y102/01008 , C12N9/0006 , C12Y101/01001
Abstract: A cyclic lipopeptide-producing microbial strain improve productivity of a cyclic lipopeptide, such as surfactin, via microbial culture. The cyclic lipopeptide-producing microbial strain, lack at least one of a gene encoding betaine aldehyde dehydrogenase (EC:1.2.1.8) or a gene encoding choline dehydrogenase (EC:1.1.1.1), and a method for producing a cyclic lipopeptide comprising culturing such microbial strain are provided.
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4.发明授权公开(公告)号：US11225676B2
公开(公告)日：2022-01-18
申请号：US16670042
申请日：2019-10-31
Applicant: KANEKA CORPORATION
Inventor： Shingo Kobayashi , Rina Aoki , Tetsuya Minami , Hisashi Arikawa , Keiji Matsumoto
Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.
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5.发明授权公开(公告)号：US10519473B2
公开(公告)日：2019-12-31
申请号：US15129726
申请日：2015-03-27
Applicant: KANEKA CORPORATION
Inventor： Shingo Kobayashi , Rina Aoki , Tetsuya Minami , Hisashi Arikawa , Keiji Matsumoto
Abstract: A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10° C. or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10° C. or more simultaneously.
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公开(公告)号：US11634696B2
公开(公告)日：2023-04-25
申请号：US17252086
申请日：2019-06-12
Applicant: KANEKA CORPORATION
Inventor： Shingo Kobayashi , Shunsuke Sato , Naoaki Taoka
Abstract: Provided is a mutant polyhydroxyalkanoate synthetase having an amino acid sequence which has 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and which contains a mutation of substitution of at least one of the amino acids at the 27th to 33rd, 39th, 56th, 106th, 129th, 144th, 165th, 170th and 172nd to 187th positions from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with another amino acid.
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公开(公告)号：US11339351B2
公开(公告)日：2022-05-24
申请号：US17058932
申请日：2019-05-27
Applicant: KANEKA CORPORATION
Inventor： Takafumi Igari , Wanying Lee , Shingo Kobayashi
Abstract: A free fatty acid-containing substance, which is a waste liquid or by-product obtained in a process for producing palm oil or which is a hydrolysate of the waste liquid or by-product, is subjected to distillation treatment to obtain a free fatty acid fraction satisfying the requirements (i), (ii) and (iii), and a polyhydroxyalkanoate-producing microorganism is cultured in a culture solution containing the free fatty acid fraction. (i) The content of squalene is 0.05 wt % or less. (ii) The ratio of a peak area of trimethylindene to a peak area of a naphthalene-d8 standard is 110 or less in first-dimensional dynamic headspace-GC/TOFMS analysis. (iii) The ratio of a peak area of butyric acid to a peak area of a naphthalene-d8 standard is less than 10 in first-dimensional dynamic headspace-GC/TOFMS analysis.
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