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公开(公告)号：US06251588B1
公开(公告)日：2001-06-26
申请号：US09021701
申请日：1998-02-10
IPC分类号： C12Q168
摘要： Methods are disclosed for predicting the potential of an oligonucleotide to hybridize to a target nucleotide sequence. A predetermined number of unique oligonucleotides is identified. The unique oligonucleotides are chosen to sample the entire length of a nucleotide sequence that is hybridizable with the target nucleotide sequence. At least one parameter that is independently predictive of the ability of each of the oligonucleotides of the set to hybridize to the target nucleotide sequence is determined and evaluated for each of the above oligonucleotides. A subset of oligonucleotides within the predetermined number of unique oligonucleotides is identified based on the evaluation of the parameter. Oligonucleotides in the subset are identified that are clustered along a region of the nucleotide sequence that is hybridizable to the target nucleotide sequence. The method may be carried out with the aid of a computer.
摘要翻译： 公开了用于预测寡核苷酸与靶核苷酸序列杂交的潜力的方法。 鉴定出预定数量的唯一寡核苷酸。 选择独特的寡核苷酸来对可与靶核苷酸序列杂交的核苷酸序列的整个长度进行采样。 确定并评估每种上述寡核苷酸的至少一个独立地预测该组寡核苷酸与靶核苷酸序列杂交的能力的参数。 基于参数的评估来鉴定预定数量的唯一寡核苷酸内的寡核苷酸的子集。 鉴定了亚群中的寡核苷酸，其沿着与靶核苷酸序列可杂交的核苷酸序列的区域聚集。 该方法可以借助于计算机进行。
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2.发明授权Methods designing multiple mRNA transcript nucleic acid probe sequences for use in nucleic acid arrays 失效设计用于核酸阵列的多个mRNA转录核酸探针序列的方法公开(公告)号：US07029854B2
公开(公告)日：2006-04-18
申请号：US10303151
申请日：2002-11-22
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , G06F19/22
摘要： Methods of identifying a sequence of a nucleic acid that is suitable for use as a surface immobilized probe for two or more mRNA transcripts encoded by the same gene are provided. In practicing the subject methods, a consensus region for the two or more transcripts is first identified, and this identified consensus region is then employed to identify the suitable nucleic acid sequence, e.g., by using a probe design protocol. The subject invention also includes algorithms for performing the subject methods recorded on a computer readable medium, as well as computational analysis systems that include the same. Also provided are nucleic acid arrays produced with probes having sequences identified by the subject methods, as well as methods for using the same.
摘要翻译： 提供了鉴定适合用作由相同基因编码的两个或更多个mRNA转录物的表面固定化探针的核酸序列的方法。 在实施本发明方法时，首先鉴定两个或多个转录物的共有区域，然后使用该鉴定的共有区域来鉴定合适的核酸序列，例如通过使用探针设计方案。 本发明还包括用于执行记录在计算机可读介质上的主题方法的算法，以及包括其的计算分析系统。 还提供了由具有由本发明方法鉴定的序列的探针产生的核酸阵列，以及使用该序列的方法。
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公开(公告)号：US06599693B1
公开(公告)日：2003-07-29
申请号：US09628963
申请日：2000-07-31
申请人： Peter G. Webb
发明人： Peter G. Webb
IPC分类号： C12Q100
CPC分类号： B01J19/0046 , Y10T436/143333
摘要： A method of fabricating an array with multiple sets of neighboring features. In the method, for each of multiple sets of neighboring features, at least one set of drops is deposited from a corresponding same pulse jet dispenser onto a substrate so as to form the array with the sets formed from drops deposited by respective different dispensers. Apparatus and computer program products which can execute a method of the invention, are also provided.
摘要翻译： 一种制造具有多组相邻特征的阵列的方法。 在该方法中，对于多组相邻特征中的每一组，至少一组液滴从相应的相同的脉冲喷射分配器沉积到基底上，以便形成阵列，其中由通过相应的不同分配器沉积的液滴形成。 还提供了可以执行本发明的方法的装置和计算机程序产品。
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公开(公告)号：US5800358A
公开(公告)日：1998-09-01
申请号：US828853
申请日：1997-03-31
CPC分类号： A61B8/06 , G01P5/244 , G01S15/582 , G01S15/18 , G01S15/8979
摘要： A motion detection scheme is described which periodically sends a plurality of pulsed ultrasonic signals from a transducer to a particular range cell to receive a series of backscattered signals from the particular range cell. The time interval between any two pulses is greater than a largest dimension of the range cell divided by a slowest velocity of motion at the particular range cell. A temporal variation between the envelopes of the signals is then determined to detect motion at the particular range cell. This scheme may be applied at all points in an image, to produce images that depict regions undergoing motion.
摘要翻译： 描述了一种运动检测方案，其周期性地将多个脉冲超声信号从换能器发送到特定范围单元以从特定范围单元接收一系列反向散射信号。 任何两个脉冲之间的时间间隔大于范围单元的最大尺寸除以特定范围单元处的最慢运动速度。 然后确定信号的包络之间的时间变化以检测特定范围单元处的运动。 该方案可以应用于图像中的所有点，以产生描绘经历运动的区域的图像。
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公开(公告)号：US08221978B2
公开(公告)日：2012-07-17
申请号：US12279570
申请日：2007-01-26
申请人： Jing Gao , B. Shane Giles , Peter G. Webb , Douglas N. Roberts
发明人： Jing Gao , B. Shane Giles , Peter G. Webb , Douglas N. Roberts
CPC分类号： G06F19/20
摘要： A method of selecting a set of normalization probes for use on a comparative genome hybridization array is provided. In certain embodiments, the method includes: a) selecting a first region of a genome to be evaluated by comparative genome hybridization to produce data; b) selecting a second region of the genome for normalization of the data, and c) selecting from a set of candidate probes a sub-set of normalization probes that detect the second region.
摘要翻译： 提供了选择用于比较基因组杂交阵列的一组归一化探针的方法。 在某些实施方案中，该方法包括：a）通过比较基因组杂交来选择待评估的基因组的第一区域以产生数据; b）选择所述基因组的第二区域以使所述数据进行归一化，以及c）从一组候选探针中选择检测所述第二区域的归一化探针的子集。
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公开(公告)号：US08074802B2
公开(公告)日：2011-12-13
申请号：US12492885
申请日：2009-06-26
申请人： Peter G. Webb , Alan Schenck , Robert E. Woodworth , Emily Marine Leproust , Eric Lin , Jeffrey M. McMillan
发明人： Peter G. Webb , Alan Schenck , Robert E. Woodworth , Emily Marine Leproust , Eric Lin , Jeffrey M. McMillan
IPC分类号： B65D85/48
CPC分类号： G02B21/34
摘要： A slide cover for enclosing a region comprising an active region on a slide is provided. A slide cover and a slide are also provided. A slide cover for enclosing a region comprising an active region on a slide, where the slide is placed in a clamshell slide holder is also provided.
摘要翻译： 提供了用于封闭包括滑动件上的活动区域的区域的滑动盖。 还提供滑盖和滑梯。 还提供一种用于封闭包括滑块上的活动区域的区域的滑动盖，其中滑动件放置在蛤壳式滑动架中。
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7.发明申请Methods and systems for pairwise filtering candidate probe nucleic acid sequences 审中-公开用于成对筛选候选探针核酸序列的方法和系统公开(公告)号：US20090036319A1
公开(公告)日：2009-02-05
申请号：US11888059
申请日：2007-07-30
摘要： Aspects of the invention include methods of systems of pairwise filtering candidate probe nucleic acid sequences. Aspects of the invention further include methods and systems of selecting candidate probe nucleic acid sequences from plurality thereof, which methods and systems employ a pairwise elimination ranked record of a plurality of candidate probe nucleic acids for a genomic region of interest.
摘要翻译： 本发明的方面包括成对筛选候选探针核酸序列的系统的方法。 本发明的方面还包括从多个选择候选探针核酸序列中选择候选探针核酸序列的方法和系统，该方法和系统采用多个候选探针核酸成对排除的记录，用于感兴趣的基因组区域。
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公开(公告)号：US20090275482A1
公开(公告)日：2009-11-05
申请号：US12279570
申请日：2007-01-26
申请人： Jing Gao , B. Shane Giles , Peter G. Webb , Douglas N. Roberts
发明人： Jing Gao , B. Shane Giles , Peter G. Webb , Douglas N. Roberts
CPC分类号： G06F19/20
摘要： A method of selecting a set of normalization probes for use on a comparative genome hybridization array is provided. In certain embodiments, the method includes: a) selecting a first region of a genome to be evaluated by comparative genome hybridization to produce data; b) selecting a second region of the genome for normalization of the data, and c) selecting from a set of candidate probes a sub-set of normalization probes that detect the second region.
摘要翻译： 提供了选择用于比较基因组杂交阵列的一组归一化探针的方法。 在某些实施方案中，该方法包括：a）通过比较基因组杂交来选择待评估的基因组的第一区域以产生数据; b）选择所述基因组的第二区域以使所述数据进行归一化，以及c）从一组候选探针中选择检测所述第二区域的归一化探针的子集。
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公开(公告)号：US07276336B1
公开(公告)日：2007-10-02
申请号：US09359527
申请日：1999-07-22
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00315 , B01J2219/0036 , B01J2219/00378 , B01J2219/00527 , B01J2219/00529 , B01J2219/0054 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00626 , B01J2219/00637 , B01J2219/00659 , B01J2219/00677 , B01J2219/00689 , B01J2219/00693 , B01J2219/00695 , B01J2219/00702 , B01J2219/00722 , B01J2219/00725 , B01L3/0241 , C40B40/06 , C40B40/10 , C40B60/14 , C40B70/00 , G06T7/0012
摘要： A method of fabricating an addressable array of biopolymer probes on a substrate according to a target array pattern using a deposition apparatus, and a deposition apparatus which can execute the method and computer program products for the apparatus. The deposition apparatus which, when operated according to a target drive pattern based on nominal operating parameters of the apparatus, provides the probes on the substrate in the target array pattern. The method includes examining at least one operating parameter for an error from a nominal value which error will result in use of the target drive pattern producing a discrepancy between the target array pattern and an actual array pattern deposited. When an error is detected deriving, based on the error, a corrected drive pattern different from the target drive pattern such that use of the corrected drive pattern results in a reduced discrepancy between the target and actual array patterns.
摘要翻译： 使用沉积装置根据目标阵列图案在基板上制造可寻址的生物聚合物探针阵列的方法，以及可执行该装置的方法和计算机程序产品的沉积装置。 沉积装置当根据装置的标称操作参数根据目标驱动模式进行操作时，以目标阵列图案在基板上提供探针。 该方法包括从标称值检查错误的至少一个操作参数，该误差将导致使用目标驱动模式，从而产生目标阵列图案与存储的实际阵列图案之间的差异。 当检测到错误时，基于该误差导出与目标驱动模式不同的校正驱动模式，使得使用校正的驱动模式导致目标和实际阵列模式之间的差异减小。
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公开(公告)号：US06998230B1
公开(公告)日：2006-02-14
申请号：US09558532
申请日：2000-04-26
申请人： Christopher A. Schantz , Kyle J. Schleifer , William D. Fisher , Richard P. Tella , Michael P. Caren , Peter G. Webb
发明人： Christopher A. Schantz , Kyle J. Schleifer , William D. Fisher , Richard P. Tella , Michael P. Caren , Peter G. Webb
CPC分类号： B82Y30/00 , B01J19/0046 , B01J2219/00378 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00621 , B01J2219/00637 , B01J2219/00639 , B01J2219/00657 , B01J2219/00659 , B01J2219/00662 , B01J2219/00664 , B01J2219/00675 , B01J2219/00677 , B01J2219/00689 , B01J2219/00691 , B01J2219/00693 , B01J2219/00695 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01L3/0268
摘要： A drop deposition apparatus, and a method and computer program products using the drop deposition apparatus, for fabricating at least one addressable array of biopolymers on a substrate. The drop deposition apparatus has a drop dispenser unit and a sensing element. The method comprises includes for each of multiple addresses, dispensing droplets carrying the biopolymers or biopolymer precursors from a drop dispenser unit onto the sensing element, and onto the substrate so as to fabricate the array. Electrical signals resulting from dispensed droplets striking the sensing element are detected. A performance characteristic of the deposition apparatus is evaluated based on the detected signals.
摘要翻译： 一种液滴沉积设备，以及使用液滴沉积设备的方法和计算机程序产品，用于在基底上制造至少一个可寻址的生物聚合物阵列。 液滴沉积装置具有液滴分配器单元和感测元件。 该方法包括对于多个地址中的每一个，将从生物聚合物或生物聚合物前体携带的液滴从液滴分配器单元分配到感测元件上，并将其分配到基底上，以制造阵列。 检测由分配的液滴引起的感测元件产生的电信号。 基于检测到的信号来评价沉积装置的性能特性。
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