利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

8 条记录 (耗时 0.031 秒)

    Method of separating and recovering proteins from a protein solution
    5.
    发明授权
    Method of separating and recovering proteins from a protein solution 有权
    从蛋白质溶液中分离和回收蛋白质的方法

    公开(公告)号：US06528622B1

    公开(公告)日：2003-03-04

    申请号：US09117401

    申请日：1999-04-30

    申请人： John Stephen Ayers ,  David Francis Elgar ,  Mark Pritchard

    发明人： John Stephen Ayers ,  David Francis Elgar ,  Mark Pritchard

    IPC分类号： C07K118

    CPC分类号： A23J3/08 ,  A23C9/1465 ,  A23J1/205 ,  Y10S530/833

    摘要： A preparative method of isolating a preselected whey protein or group of whey proteins from a solution is provided. The method comprises the following steps: (a) contacting a whey protein solution with the preselected ion exchanger for a time and at a temperature sufficient to enable the preselected whey protein to be adsorbed; wherein the whey protein solution has (1) a protein content in the range of about 5% to about 20% by weight, (2) a pH of a preselected level, which is the level at which the preselected whey protein or group of whey proteins selectively binds to the preselected ion exchanger, and (3) a reduced ionic strength; and (b) recovering either or both of the following: (1) the whey protein component adsorbed in step (a), and (2) the breakthrough whey protein component not adsorbed in step (a). It is preferred that the whey protein solution is a retentate obtained via ultrafiltration of whey, having reduced ionic strength, or a whey protein concentrate powder which has been reconstituted with water.

    摘要翻译： 提供了从溶液中分离预选乳清蛋白或一组乳清蛋白的制备方法。 该方法包括以下步骤：（a）将乳清蛋白溶液与预选离子交换剂接触一段时间并且足以使预选乳清蛋白被吸附的温度; 其中所述乳清蛋白溶液具有（1）蛋白质含量在约5％至约20％重量的范围内，（2）预选水平的pH，其是预选乳清蛋白或乳清组的水平 蛋白质选择性地结合预选离子交换剂，和（3）降低的离子强度; 和（b）回收下列各项之一或两者：（1）步骤（a）中吸附的乳清蛋白组分，和（2）步骤（a）中未吸附的穿透乳清蛋白组分。 优选乳清蛋白溶液是通过具有降低的离子强度的乳清超滤获得的保留物，或已经用水重构的乳清蛋白浓缩物粉末。

    Production of an immunoglobulin enriched fraction from whey protein solutions
    7.
    发明授权
    Production of an immunoglobulin enriched fraction from whey protein solutions 失效
    从乳清蛋白溶液中制备免疫球蛋白富集级分

    公开(公告)号：US06592905B1

    公开(公告)日：2003-07-15

    申请号：US09117631

    申请日：1998-11-16

    申请人： John Stephen Ayers ,  David Francis Elgar ,  Mark Pritchard

    发明人： John Stephen Ayers ,  David Francis Elgar ,  Mark Pritchard

    IPC分类号： A61K3520

    CPC分类号： A23J1/205 ,  A23C9/1465

    摘要： This invention is directed to a process for producing an immunoglobulin enriched whey protein fraction from whey protein solutions. More particularly, it relates to the production of a whey protein fraction enriched in immunoglobulin, and optionally a whey protein isolate, using a cation exchanger under selected conditions. The selected conditions require overloading the cation exchanger with potentially absorbable protein which causes the exchanger to adsorb preferentially whey proteins other than immunoglobulin. The invention is also directed to the products produced by the process of the invention.

    摘要翻译： 本发明涉及一种从乳清蛋白溶液中生产免疫球蛋白的乳清蛋白级分的方法。 更具体地说，本发明涉及使用阳离子交换剂在选择的条件下生产富含免疫球蛋白的乳清蛋白质级分和任选的乳清蛋白分离物。 所选择的条件需要使潜在的可吸收蛋白质使阳离子交换剂过载，这导致交换剂优先吸附除免疫球蛋白以外的乳清蛋白。 本发明还涉及通过本发明的方法生产的产品。

    Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5% w/w
    8.
    发明授权
    Process for isolating glycomacropeptide from dairy products with a phenylalanine impurity of 0.5% w/w 失效
    从具有0.5％w / w的苯丙氨酸杂质的乳制品中分离糖巨肽的方法

    公开(公告)号：US06555659B1

    公开(公告)日：2003-04-29

    申请号：US09625043

    申请日：2000-07-24

    申请人： John Stephen Ayers ,  Kay Patricia Coolbear ,  David Francis Elgar ,  Mark Pritchard

    发明人： John Stephen Ayers ,  Kay Patricia Coolbear ,  David Francis Elgar ,  Mark Pritchard

    IPC分类号： C07K1400

    CPC分类号： A23J1/202 ,  C07K14/4732 ,  Y10S530/832 ,  Y10S530/833

    摘要： The present invention is related to a method for the purification of glycomacropeptide (GMP) with an amino acid composition containing no greater that 0.5% (w/w) phenylalanine, comprising the steps of contacting a GMP-containing feedstock with a first anion exchanger under conditions to adsorb the GMP, eluting the adsorbed GMP from the anion exchanger and removing impurities from the GMP-containing eluate by either: (i) contacting the GMP-containing eluate with a cation exchanger in conditions under which the impurities in the eluate are adsorbed onto the cation exchanger, or (ii) precipitating the impurities in GMP-containing eluate using conditions in which the GMP remains i solution, or (iii) contacting the GMP-containing eluate with a second anion exchanger in conditions under which the impurities in the eluate are adsorbed onto the anion exchanger, and recovering the GMP from whichever one or more of the steps (i), (ii) or (iii) was used.

    摘要翻译： 本发明涉及一种纯化含有不大于0.5％（w / w）苯丙氨酸的氨基酸组成的糖巨肽（GMP）的方法，该方法包括以下步骤：使含GMP的原料与第一阴离子交换剂接触 吸附GMP的条件，从阴离子交换器洗脱吸附的GMP并从含GMP的洗脱液中除去杂质，方法是：（i）在吸附洗脱液中的杂质的条件下将含GMP的洗脱液与阳离子交换剂接触 或者（ii）使用GMP保留在溶液中的条件沉淀含GMP的洗脱液中的杂质，或者（iii）使含GMP的洗脱液与第二阴离子交换剂接触， 洗脱液被吸附到阴离子交换器上，并且从使用步骤（i），（ii）或（iii）中的一个或多个步骤回收GMP。