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13 条记录 (耗时 0.036 秒)

    Human p51 genes and gene products thereof
    3.
    发明授权
    Human p51 genes and gene products thereof 有权
    人p51基因及其基因产物

    公开(公告)号：US07964367B2

    公开(公告)日：2011-06-21

    申请号：US12786018

    申请日：2010-05-24

    申请人： Yoji Ikawa ,  Shuntaro Ikawa ,  Masuo Obinata

    发明人： Yoji Ikawa ,  Shuntaro Ikawa ,  Masuo Obinata

    IPC分类号： G01N33/574

    CPC分类号： C07K14/4747 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4746

    摘要： Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.

    摘要翻译： 属于与已知为细胞增殖调节基因的p53基因有关的家族基因类别的新型人类基因及其基因产物。 特征在于包含编码SEQ ID NO：1所示的氨基酸序列的碱基序列的人p51基因; 具有由SEQ ID NO：2所示的序列中的145〜1488个碱基组成的碱基序列的人p51基因; 含有这些基因的载体; 用这些载体转化的宿主细胞; 制备具有SEQ ID NO：1所示氨基酸序列的p51蛋白质的方法; 其包括培养上述宿主细胞并从由此获得的培养物中收获蛋白质; 和具有SEQ ID NO：1所示的氨基酸序列的p51蛋白。

    Human lymphotoxin
    4.
    发明授权
    Human lymphotoxin 失效
    人淋巴毒素

    公开(公告)号：US5776446A

    公开(公告)日：1998-07-07

    申请号：US243168

    申请日：1994-05-16

    申请人： Toshiaki Osawa ,  Masuo Obinata ,  Yoshiyuki Ishii ,  Yoshio Kobayashi

    发明人： Toshiaki Osawa ,  Masuo Obinata ,  Yoshiyuki Ishii ,  Yoshio Kobayashi

    IPC分类号： A61K38/00 ,  C07K14/525 ,  A61K38/19 ,  C07K14/52

    CPC分类号： C07K14/5255 ,  A61K38/00

    摘要： Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the base sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.

    摘要翻译： 产生淋巴毒素的人T细胞杂交瘤在含有佛波酮肉豆蔻酸乙酸酯，刀豆杆菌素A或其混合物的培养基中培养，所得细胞通过蔗糖密度梯度离心法分级，以将12.6S至14.6S级分中的信使RNA ，并且从信使RNA制备含有编码具有淋巴毒素活性的多肽的部分的基因。 该基因由本文给出的表1的碱基序列表示。 使用本发明的基因，可以通过遗传工程技术获得具有本文给出的氨基酸序列（I）或（II）的新的淋巴毒素。

    Human p51 genes and gene products thereof
    5.
    发明授权
    Human p51 genes and gene products thereof 有权
    人p51基因及其基因产物

    公开(公告)号：US07132276B1

    公开(公告)日：2006-11-07

    申请号：US09670568

    申请日：1999-03-24

    申请人： Yoji Ikawa ,  Shuntaro Ikawa ,  Masuo Obinata

    发明人： Yoji Ikawa ,  Shuntaro Ikawa ,  Masuo Obinata

    IPC分类号： C12N15/63

    CPC分类号： C07K14/4747 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4746

    摘要： Novel human genes falling whithin the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.

    摘要翻译： 与被称为细胞增殖调节基因的p53基因有关的家族基因类别及其基因产物的新型人类基因。 特征在于包含编码SEQ ID NO：1所示的氨基酸序列的碱基序列的人p51基因; 具有由SEQ ID NO：2所示的序列中的145〜1488个碱基组成的碱基序列的人p51基因; 含有这些基因的载体; 用这些载体转化的宿主细胞; 制备具有SEQ ID NO：1所示氨基酸序列的p51蛋白质的方法; 其包括培养上述宿主细胞并从由此获得的培养物中收获蛋白质; 和具有SEQ ID NO：1所示的氨基酸序列的p51蛋白。

    Method for production of lymphotoxin (TNFB) in cell line A-C5-8
    6.
    发明授权
    Method for production of lymphotoxin (TNFB) in cell line A-C5-8 失效
    在细胞系A-C5-8中产生淋巴毒素（TNFB）的方法

    公开(公告)号：US5403725A

    公开(公告)日：1995-04-04

    申请号：US733974

    申请日：1991-07-22

    申请人： Toshiaki Osawa ,  Masuo Obinata ,  Yoshiyuki Ishii ,  Yoshio Kobayashi

    发明人： Toshiaki Osawa ,  Masuo Obinata ,  Yoshiyuki Ishii ,  Yoshio Kobayashi

    IPC分类号： A61K38/00 ,  C07K14/525 ,  A61K45/05 ,  C12N15/00 ,  C12P21/06

    CPC分类号： C07K14/5255 ,  A61K38/00

    摘要： Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the nucleotide sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.

    摘要翻译： 产生淋巴毒素的人T细胞杂交瘤在含有佛波酮肉豆蔻酸乙酸酯，刀豆杆菌素A或其混合物的培养基中培养，所得细胞通过蔗糖密度梯度离心法分级，以将12.6S至14.6S级分中的信使RNA 并且从信使RNA制备含有编码具有淋巴毒素活性的多肽的部分的基因。 该基因由本文给出的表1的核苷酸序列表示。 使用本发明的基因，可以通过遗传工程技术获得具有本文给出的氨基酸序列（I）或（II）的新的淋巴毒素。

    Human p51 genes and gene products thereof
    7.
    发明授权
    Human p51 genes and gene products thereof 有权
    人p51基因及其基因产物

    公开(公告)号：US07553933B2

    公开(公告)日：2009-06-30

    申请号：US11340715

    申请日：2006-01-27

    申请人： Yoji Ikawa ,  Shuntaro Ikawa ,  Masuo Obinata

    发明人： Yoji Ikawa ,  Shuntaro Ikawa ,  Masuo Obinata

    IPC分类号： C07K14/00

    CPC分类号： C07K14/4747 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4746

    摘要： Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.

    摘要翻译： 属于与已知为细胞增殖调节基因的p53基因有关的家族基因类别的新型人类基因及其基因产物。 特征在于包含编码SEQ ID NO：1所示的氨基酸序列的碱基序列的人p51基因; 具有由SEQ ID NO：2所示的序列中的145〜1488个碱基组成的碱基序列的人p51基因; 含有这些基因的载体; 用这些载体转化的宿主细胞; 制备具有SEQ ID NO：1所示氨基酸序列的p51蛋白质的方法; 其包括培养上述宿主细胞并从由此获得的培养物中收获蛋白质; 和具有SEQ ID NO：1所示的氨基酸序列的p51蛋白。

    Human p51 genes and gene products thereof
    10.
    发明授权
    Human p51 genes and gene products thereof 有权
    人p51基因及其基因产物

    公开(公告)号：US07754857B2

    公开(公告)日：2010-07-13

    申请号：US12169213

    申请日：2008-07-08

    申请人： Yoji Ikawa ,  Shuntaro Ikawa ,  Masuo Obinata

    发明人： Yoji Ikawa ,  Shuntaro Ikawa ,  Masuo Obinata

    IPC分类号： C07K16/00 ,  G01N33/536

    CPC分类号： C07K14/4747 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4746

    摘要： Novel human genes falling within the category of family genes relating to p53 gene which is known as a cell proliferation regulatory gene, and gene products thereof. A human p51 gene characterized by containing a base sequence encoding an amino acid sequence represented by SEQ ID NO:1; a human p51 gene having a base sequence consisting of the 145- to 1488-bases in the sequence represented by SEQ ID NO:2; vectors containing these genes; host cells transformed with these vectors; a process for producing a p51 protein having the amino sequence represented by SEQ ID NO:1; which comprises culturing the above host cells and harvesting the protein from the thus obtained culture; and the p51 protein having the amino acid sequence represented by SEQ ID NO:1.

    摘要翻译： 属于与已知为细胞增殖调节基因的p53基因有关的家族基因类别的新型人类基因及其基因产物。 特征在于包含编码SEQ ID NO：1所示的氨基酸序列的碱基序列的人p51基因; 具有由SEQ ID NO：2所示的序列中的145〜1488个碱基组成的碱基序列的人p51基因; 含有这些基因的载体; 用这些载体转化的宿主细胞; 制备具有SEQ ID NO：1所示氨基酸序列的p51蛋白质的方法; 其包括培养上述宿主细胞并从由此获得的培养物中收获蛋白质; 和具有SEQ ID NO：1所示的氨基酸序列的p51蛋白。