公开(公告)号：US5057438A

公开(公告)日：1991-10-15

申请号：US31665

申请日：1987-03-30

申请人： Kazumichi Imai ,  Daizo Tokinaga ,  Teruaki Kobayashi ,  Kenji Yasuda ,  Keiichi Nagai ,  Satoshi Takahashi

发明人： Kazumichi Imai ,  Daizo Tokinaga ,  Teruaki Kobayashi ,  Kenji Yasuda ,  Keiichi Nagai ,  Satoshi Takahashi

IPC分类号： G01N33/561

CPC分类号： G01N33/561 ,  Y10S436/809 ,  Y10S436/824

摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.

摘要翻译： 通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定 反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体 并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。

公开(公告)号：US4824778A

公开(公告)日：1989-04-25

申请号：US867554

申请日：1986-05-28

申请人： Keiichi Nagai ,  Daizo Tokinaga ,  Kazumichi Imai ,  Kenji Yasuda ,  Satoshi Takahashi ,  Teruaki Kobayashi

发明人： Keiichi Nagai ,  Daizo Tokinaga ,  Kazumichi Imai ,  Kenji Yasuda ,  Satoshi Takahashi ,  Teruaki Kobayashi

IPC分类号： G01N33/543 ,  G01N33/561 ,  G01N33/53

CPC分类号： G01N33/561 ,  Y10S435/968

摘要： An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluorescent substance in the electrolyte solution.

摘要翻译： 免疫测定法，其包括将抗体固定在用于电泳的基质中; 通过使抗原通过电泳移动的方法，将抗原固定在测量样品中，使其与上述固定化抗体进行抗原抗体反应; 通过电泳将标记抗体移动到上述固定化抗原上，使其与固定化抗原反应，或通过电泳将标记的抗原移动到上述固定化抗体的未反应部分，使其与未反应部分反应; 测定样品中的抗原浓度，其特征在于，将标记抗体或标记抗原的标记作为能够将底物覆盖成荧光物质的酶，通过电泳将所述酶转化为荧光物质， 使底物与标记酶反应，将其转化为荧光物质，并测量电解质溶液中荧光物质的浓度。

公开(公告)号：US4628035A

公开(公告)日：1986-12-09

申请号：US638423

申请日：1984-08-07

申请人： Daizo Tokinaga ,  Teruaki Kobayashi ,  Kazumichi Imai

发明人： Daizo Tokinaga ,  Teruaki Kobayashi ,  Kazumichi Imai

IPC分类号： A61K39/44 ,  G01N33/561 ,  G01N33/58 ,  G01N33/543

CPC分类号： G01N33/561

摘要： An immunoassay method for measuring a concentration of an antigen for a short period of time by immobilizing an antibody over the whole zone of an effective supporting matrix for electrophoresis and fixing an antigen in a sample to be measured by electrophoresis for the antigen-antibody reaction between said immobilized antibody and said antigen.

摘要翻译： 一种免疫测定方法，用于通过将抗体固定在有效支持基质的整个区域上进行电泳和固定待测样品中的抗原，将抗体固定在短时间内的抗原浓度，以通过电泳进行抗原抗体反应 所述固定化抗体和所述抗原。

公开(公告)号：US4913883A

公开(公告)日：1990-04-03

申请号：US221971

申请日：1988-07-20

申请人： Kazumichi Imai ,  Daizo Tokinaga ,  Koichi Yokosawa

发明人： Kazumichi Imai ,  Daizo Tokinaga ,  Koichi Yokosawa

IPC分类号： G01N33/543 ,  G01N35/00

CPC分类号： G01N33/54333 ,  G01N33/54366 ,  G01N35/0098

摘要： The degree of agglutination is measured in a magnetic manner instead of optical manner. Microparticles are composed of a magnetic material and the agglutinated condition is measured using a magnetometer. The sizes and distribution of agglutinated materials formed by the antigen-antibody coupling reaction can be correctly measured without affected by light-scattering materials such as proteins or blood cells contained in the specimen, or by absorbant such as pigment, or by fluorescent material. Therefore, the concentration of antigen is measured maintaining high precision.

摘要翻译： 以磁性方式代替光学方式测量凝集度。 微粒由磁性材料组成，并且使用磁力计测量凝集条件。 通过抗原 - 抗体偶联反应形成的凝集物质的大小和分布可以在不受光散射物质如样品中所含的蛋白质或血液细胞，或吸收剂如颜料或荧光材料的影响的情况下被正确地测量。 因此，保持高精度地测量抗原的浓度。

公开(公告)号：US20130157264A1

公开(公告)日：2013-06-20

申请号：US13818704

申请日：2011-07-19

申请人： Takayuki Obara ,  Kazumichi Imai ,  Toshiro Saito ,  Satoshi Takahashi

发明人： Takayuki Obara ,  Kazumichi Imai ,  Toshiro Saito ,  Satoshi Takahashi

IPC分类号： G01N21/64

CPC分类号： G01N21/6486 ,  C12Q1/6837 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/648 ,  C12Q2563/173

摘要： In a nucleic acid analysis device which detects a fluorescent dye on a nucleic acid sample immobilized on a surface of a substrate by exciting the fluorescent dye with an evanescent wave, the detection of a fluorescence signal with a high SN ratio is realized even for a long nucleic acid sample.The nucleic acid analysis device according to the invention is a nucleic acid analysis device in which a plurality of regions for immobilizing a nucleic acid sample are provided on a surface of a support base and a single molecule of a nucleic acid sample is immobilized on at least one of the regions, and which performs sequence determination by performing an extension reaction of the immobilized nucleic acid sample, wherein the immobilization of the single molecule of the nucleic acid sample on the support base is performed at two or more points.

摘要翻译： 在通过用ev逝波激发荧光染料来检测固定在基板表面上的核酸样品上的荧光染料的核酸分析装置中，即使长时间地实现高SN比的荧光信号的检测 核酸样品。 根据本发明的核酸分析装置是核酸分析装置，其中在支持基底的表面上提供用于固定核酸样品的多个区域，并且将至少一个核酸样品的单个分子固定在至少 其中一个区域，并且通过进行固定化的核酸样品的延伸反应进行序列测定，其中将核酸样品的单分子固定在载体基底上进行两点或更多点。

公开(公告)号：US07662269B2

公开(公告)日：2010-02-16

申请号：US12359115

申请日：2009-01-23

申请人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

发明人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

IPC分类号： G01N27/453

CPC分类号： G01N27/44713 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782

摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.

摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。 通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

公开(公告)号：US20090134030A1

公开(公告)日：2009-05-28

申请号：US12359115

申请日：2009-01-23

申请人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

发明人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

IPC分类号： G01N27/447

CPC分类号： G01N27/44713 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782

摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.

摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。 通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

公开(公告)号：US07014746B2

公开(公告)日：2006-03-21

申请号：US10413540

申请日：2003-04-15

申请人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

发明人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

IPC分类号： G01N27/453

CPC分类号： G01N27/44713 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782

摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries.By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.

摘要翻译： 通过构成电连接到公共电极的电极对和毛细管来消除在多个毛细管的设置期间的麻烦。 通过将安装在每个毛细管附近的电极安装在样品板侧面（在该区域内）的孔的间距与公共电极电接触，毛细管和电极在结构上是一体的。 当通过公共电极部分向电泳仪施加电压时，对每个毛细管的电极施加电压。 这使得可以使用廉价的微量滴定板等，同时插入，附着和分离多个毛细管。

公开(公告)号：US20140200162A1

公开(公告)日：2014-07-17

申请号：US14233256

申请日：2012-05-16

申请人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

发明人： Toshiro Saito ,  Koshin Hamasaki ,  Satoshi Takahashi ,  Muneo Maeshima ,  Kyoko Imai ,  Kazumichi Imai ,  Ryuji Tao

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6834 ,  C12N15/1065 ,  C12Q2525/207 ,  C12Q2537/125 ,  C12Q2537/143 ,  C12Q2563/143 ,  C12Q2563/149

摘要： A convenient method for nucleic acid analysis is provided, which enables 1000 or more types of nucleic acid to be analyzed collectively with high comprehensiveness and with a dynamic range of at least four digits. In particular, provided is a very effective analytical method especially for untranslated RNAs and microRNAs, of which the types of target nucleic acids is 10000 or lower. Nucleic acids can be analyzed conveniently and rapidly with high comprehensiveness and quantitative performance at single-molecule sensitivity and resolution by following the steps of: preparing a group of target nucleic acid fragments one molecule at a time and hybridizing the nucleic acid molecules, which have known base sequences and have been labeled with the fluorescence substances, with the group of the target nucleic acid fragments to detect the fluorescence substances labeling the hybridized nucleic acid molecules.

摘要翻译： 提供了一种方便的核酸分析方法，使得能够以高全面性和至少四位数的动态范围共同分析1000种或更多种类型的核酸。 特别地，提供了非常有效的分析方法，特别是对于非翻译的RNA和微小RNA，其靶核酸的类型为10000或更低。 可以通过以下步骤以单分子灵敏度和分辨率的高全面性和定量性能方便快速地分析核酸：一次一个分子制备一组靶核酸片段，并将已知的核酸分子杂交 碱基序列，并用荧光物质标记，用靶核酸片段组来检测标记杂交核酸分子的荧光物质。

公开(公告)号：US20060086612A1

公开(公告)日：2006-04-27

申请号：US11296487

申请日：2005-12-08

申请人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

发明人： Muneo Maeshima ,  Kazumichi Imai ,  Masaya Kojima ,  Satoshi Takahashi ,  Hiromi Yamashita

IPC分类号： G01N27/447

CPC分类号： G01N27/44713 ,  G01N27/447 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44782

摘要： The troublesomeness during the setting of a plurality of capillaries is eliminated by composing pairs of electrodes, which are electrically connected to the common electrode, and capillaries. By bringing electrodes installed in the vicinity of each capillary disposed at the pitch of wells on the side of sample plate (within the area of the wells) into electrical contact with a common electrode, the capillaries and electrodes are made integral in construction. When a voltage is applied to the electrophoretic instrument via a common electrode portion, the voltage is applied to the electrodes for each capillary. This enables an inexpensive microtiter plate, etc. to be used and a multiple of capillaries to be simultaneously inserted, attached and detached.