公开(公告)号：US5057438A

公开(公告)日：1991-10-15

申请号：US31665

申请日：1987-03-30

申请人： Kazumichi Imai ,  Daizo Tokinaga ,  Teruaki Kobayashi ,  Kenji Yasuda ,  Keiichi Nagai ,  Satoshi Takahashi

发明人： Kazumichi Imai ,  Daizo Tokinaga ,  Teruaki Kobayashi ,  Kenji Yasuda ,  Keiichi Nagai ,  Satoshi Takahashi

IPC分类号： G01N33/561

CPC分类号： G01N33/561 ,  Y10S436/809 ,  Y10S436/824

摘要： Determination of a plurality of species of antibodies or antigens is attained by a method which comprises forming a plurality of different kinds of reaction membranes each having a different species of antibody or antigen on an electrophoretic carrier, superposing these reaction membranes, optionally superposing a filter on the laminate of reaction membranes, inserting the laminate in an electrolyte, adding a plurality of different species of antigens or antibodies corresponding to the plurality of species of antibodies or antigens supported in the aforementioned reaction membranes, electrophoretically moving the added antigens or antibodies through the electrolyte and enabling them to react with the antibodies or antigens supported on the reaction membranes, and measuring the concentrations of either the antigens or antibodies resulting from the reaction or the antibodies or antigens supported in an unreacted form on the reaction membranes.

摘要翻译： 通过包括在电泳载体上形成多个不同种类的抗体或抗原的不同种类的反应膜，叠加这些反应膜，任选地将过滤器叠加在一起的方法来实现多种抗体或抗原的测定 反应膜的层压体，将层压体插入电解质中，加入与上述反应膜中支持的多种抗体或抗原相对应的多种不同种类的抗原或抗体，通过电解质电泳移动加入的抗原或抗体 并使其能够与支持在反应膜上的抗体或抗原反应，并测量由反应产生的抗原或抗体的浓度，或反应膜上以未反应形式负载的抗体或抗原的浓度。

公开(公告)号：US4824778A

公开(公告)日：1989-04-25

申请号：US867554

申请日：1986-05-28

申请人： Keiichi Nagai ,  Daizo Tokinaga ,  Kazumichi Imai ,  Kenji Yasuda ,  Satoshi Takahashi ,  Teruaki Kobayashi

发明人： Keiichi Nagai ,  Daizo Tokinaga ,  Kazumichi Imai ,  Kenji Yasuda ,  Satoshi Takahashi ,  Teruaki Kobayashi

IPC分类号： G01N33/543 ,  G01N33/561 ,  G01N33/53

CPC分类号： G01N33/561 ,  Y10S435/968

摘要： An immunoassay comprisingimmobilizing antibody in a matrix for electrophoresis;immobilizing antigen in a measurement sample by subjecting the same to antigen antibody reaction with the above-mentioned immobilized antibody by a procedure of moving the antigen by electrophoresis;either moving labeled antibody to the above-mentioned immobilized antigen by electrophoresis to react the same with the immobilized antigen, or moving labeled antigen to the unreacted portion of the above-mentioned immobilized antibody by electrophoresis to react the same with the unreacted portion; andmeasuring the concentration of antigen in the sample, characterized byusing as a label for the labeled antibody or the labeled antigen an enzyme capable of coverting a substrate into a fluorescent substance,moving the substrate convertible into a fluorescent substance by said enzyme by electrophoresis,reacting the substrate with the label enzyme to convert the same into a fluorescent substance, andmeasuring the concentration of the fluorescent substance in the electrolyte solution.

摘要翻译： 免疫测定法，其包括将抗体固定在用于电泳的基质中; 通过使抗原通过电泳移动的方法，将抗原固定在测量样品中，使其与上述固定化抗体进行抗原抗体反应; 通过电泳将标记抗体移动到上述固定化抗原上，使其与固定化抗原反应，或通过电泳将标记的抗原移动到上述固定化抗体的未反应部分，使其与未反应部分反应; 测定样品中的抗原浓度，其特征在于，将标记抗体或标记抗原的标记作为能够将底物覆盖成荧光物质的酶，通过电泳将所述酶转化为荧光物质， 使底物与标记酶反应，将其转化为荧光物质，并测量电解质溶液中荧光物质的浓度。

公开(公告)号：US4628035A

公开(公告)日：1986-12-09

申请号：US638423

申请日：1984-08-07

申请人： Daizo Tokinaga ,  Teruaki Kobayashi ,  Kazumichi Imai

发明人： Daizo Tokinaga ,  Teruaki Kobayashi ,  Kazumichi Imai

IPC分类号： A61K39/44 ,  G01N33/561 ,  G01N33/58 ,  G01N33/543

CPC分类号： G01N33/561

摘要： An immunoassay method for measuring a concentration of an antigen for a short period of time by immobilizing an antibody over the whole zone of an effective supporting matrix for electrophoresis and fixing an antigen in a sample to be measured by electrophoresis for the antigen-antibody reaction between said immobilized antibody and said antigen.

摘要翻译： 一种免疫测定方法，用于通过将抗体固定在有效支持基质的整个区域上进行电泳和固定待测样品中的抗原，将抗体固定在短时间内的抗原浓度，以通过电泳进行抗原抗体反应 所述固定化抗体和所述抗原。

公开(公告)号：US4913883A

公开(公告)日：1990-04-03

申请号：US221971

申请日：1988-07-20

申请人： Kazumichi Imai ,  Daizo Tokinaga ,  Koichi Yokosawa

发明人： Kazumichi Imai ,  Daizo Tokinaga ,  Koichi Yokosawa

IPC分类号： G01N33/543 ,  G01N35/00

CPC分类号： G01N33/54333 ,  G01N33/54366 ,  G01N35/0098

摘要： The degree of agglutination is measured in a magnetic manner instead of optical manner. Microparticles are composed of a magnetic material and the agglutinated condition is measured using a magnetometer. The sizes and distribution of agglutinated materials formed by the antigen-antibody coupling reaction can be correctly measured without affected by light-scattering materials such as proteins or blood cells contained in the specimen, or by absorbant such as pigment, or by fluorescent material. Therefore, the concentration of antigen is measured maintaining high precision.

摘要翻译： 以磁性方式代替光学方式测量凝集度。 微粒由磁性材料组成，并且使用磁力计测量凝集条件。 通过抗原 - 抗体偶联反应形成的凝集物质的大小和分布可以在不受光散射物质如样品中所含的蛋白质或血液细胞，或吸收剂如颜料或荧光材料的影响的情况下被正确地测量。 因此，保持高精度地测量抗原的浓度。

公开(公告)号：US4476005A

公开(公告)日：1984-10-09

申请号：US537511

申请日：1983-09-30

申请人： Daizo Tokinaga ,  Teruaki Kobayashi ,  Akiko Katori ,  Yoshiharu Karasawa

发明人： Daizo Tokinaga ,  Teruaki Kobayashi ,  Akiko Katori ,  Yoshiharu Karasawa

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/30 ,  C12Q1/58

CPC分类号： C12Q1/002 ,  C12Q1/005 ,  Y10S435/817

摘要： Urea electrode comprises a urease-immobilized membrane having added amino groups prepared by dipping an immobilized membrane in an aqueous solution of ethylenediamine or polylysine and an electrode for quantitative determination of ammonium ions. The electrode has a shorter measurement time with a larger electrode output.

摘要翻译： 尿素电极包括固定有脲酶的膜，其具有通过将固定化膜浸渍在乙二胺或聚赖氨酸的水溶液中的添加的氨基和用于定量测定铵离子的电极。 电极具有较短的测量时间，较大的电极输出。

公开(公告)号：US07316770B2

公开(公告)日：2008-01-08

申请号：US09814860

申请日：2001-03-23

申请人： Ryoji Inaba ,  Tomoyuki Sakai ,  Satoshi Takahashi ,  Muneo Maeshima ,  Miho Ozawa ,  Masaya Kojima ,  Daizo Tokinaga

发明人： Ryoji Inaba ,  Tomoyuki Sakai ,  Satoshi Takahashi ,  Muneo Maeshima ,  Miho Ozawa ,  Masaya Kojima ,  Daizo Tokinaga

IPC分类号： G01N27/26

CPC分类号： G01N27/44708 ,  G01N27/44782

摘要： A method of electrophoresis, an electrophoresis apparatus and a capillary array in which a fluorescently labelled sample is supplied into capillaries in a capillary array constituted by a plurality of capillaries and including a sample supply portion, an electrophoresis medium supply portion and a detection portion while controlling the temperature of the capillary array through gas circulation, the sample is caused to migrate and separated in the capillaries through electrophoresis, and at the region where the capillaries are contacted or come close each other a plurality of capillaries are contacted to a solid body so as to dissipate heat generated from the capillaries to the solid body.

摘要翻译： 一种电泳方法，电泳装置和毛细管阵列，其中荧光标记的样品被供应到由多个毛细管构成的毛细管阵列的毛细管中，并且包括样品供应部分，电泳介质供应部分和检测部分，同时控制 通过气体循环的毛细管阵列的温度，通过电泳使样品在毛细管中迁移和分离，并且在毛细管接触或彼此靠近的区域，多个毛细管与固体接触，以便 以将从毛细管产生的热量散发到固体。

公开(公告)号：US5308990A

公开(公告)日：1994-05-03

申请号：US882954

申请日：1992-05-14

申请人： Satoshi Takahashi ,  Daizo Tokinaga ,  Kazunori Okano

发明人： Satoshi Takahashi ,  Daizo Tokinaga ,  Kazunori Okano

IPC分类号： F02B75/02 ,  G01N15/14

CPC分类号： G01N15/1459 ,  F02B2075/027 ,  G01N2015/0092 ,  G01N2015/1486 ,  G01N2021/6439

摘要： A microparticle measuring method according to the present invention, by which the number of fluorescent microparticles is counted and the fluorescent microparticles are analyzed, includes the steps of introducing fluorescent microparticles into a narrow flow path almost one after another; irradiating the fluorescent microparticles in the narrow flow path with excitation light; detecting a signal pulse produced by detection of a single photon of fluorescence generated by the irradiation with the excitation light; and recognizing existence of the fluorescent microparticle, starting from the number of signal pulses measured per predetermined standard period, and further includes the step of obtaining the number of signal pulses per standard period with a time interval shorter than the standard period. It further includes the step of counting successively the number of signal pulses generated in the predetermined standard period to recognize existence of the fluorescent microparticle, when the count value exceeds a predetermined threshold, and the kind of the fluorescent microparticles is estimated from the count value. Particularly by using microparticles having a diameter smaller than 0.1 .mu.m as label material, reaction efficiency of the label material is increased, stability of the binding with the material to be measured is raised, and the material to be measured can be detected with a high precision and a high sensitivity.

摘要翻译： 根据本发明的微粒测量方法，其中计数荧光微粒的数量并分析荧光微粒，包括几乎一个接一个地将荧光微粒引入窄流路的步骤; 用激发光照射窄流路中的荧光微粒; 检测通过用激发光照射产生的荧光的单个光子的检测而产生的信号脉冲; 并且从每个预定标准周期测量的信号脉冲数开始识别荧光微粒的存在，并且还包括以比标准周期短的时间间隔获得每标准周期的信号脉冲数的步骤。 还包括当计数值超过预定阈值时，连续计数在预定标准周期中产生的信号脉冲数，以识别荧光微粒的存在的步骤，并根据计数值估计荧光微粒的种类。 特别是通过使用直径小于0.1μm的微粒作为标签材料，标签材料的反应效率提高，与被测定材料的结合的稳定性提高，可以高检出被测量的材料 精度高，灵敏度高。

公开(公告)号：US08142635B2

公开(公告)日：2012-03-27

申请号：US12843103

申请日：2010-07-26

申请人： Yasushi Shimizu ,  Toshiaki Kita ,  Kiyoshi Tsukada ,  Akihiro Suzuki ,  Tomonari Morioka ,  Masaya Kojima ,  Ryoji Inaba ,  Daizo Tokinaga ,  Shuhei Yamamoto ,  Yoshiyuki Okishima ,  Masao Fukunaga ,  Takayasu Furukawa ,  Tomohiro Shoji

发明人： Yasushi Shimizu ,  Toshiaki Kita ,  Kiyoshi Tsukada ,  Akihiro Suzuki ,  Tomonari Morioka ,  Masaya Kojima ,  Ryoji Inaba ,  Daizo Tokinaga ,  Shuhei Yamamoto ,  Yoshiyuki Okishima ,  Masao Fukunaga ,  Takayasu Furukawa ,  Tomohiro Shoji

IPC分类号： C02F1/40

CPC分类号： G01N27/44721 ,  G01N27/44782

摘要： A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.

摘要翻译： 毛细管阵列包括作为电泳所需功能的光检测部分，样品供应部分，缓冲溶液供应部分和电压施加部分，从而当将毛细管阵列组装到电泳装置中时可以立即使用。 因此，提供了可以容易地并入电泳装置中的毛细管阵列。

公开(公告)号：US07785458B2

公开(公告)日：2010-08-31

申请号：US11300377

申请日：2005-12-15

申请人： Yasushi Shimizu ,  Toshiaki Kita ,  Kiyoshi Tsukada ,  Akihiro Suzuki ,  Tomonari Morioka ,  Masaya Kojima ,  Ryoji Inaba ,  Daizo Tokinaga ,  Shuhei Yamamoto ,  Yoshiyuki Okishima ,  Masao Fukunaga ,  Takayasu Furukawa ,  Tomohiro Shoji

发明人： Yasushi Shimizu ,  Toshiaki Kita ,  Kiyoshi Tsukada ,  Akihiro Suzuki ,  Tomonari Morioka ,  Masaya Kojima ,  Ryoji Inaba ,  Daizo Tokinaga ,  Shuhei Yamamoto ,  Yoshiyuki Okishima ,  Masao Fukunaga ,  Takayasu Furukawa ,  Tomohiro Shoji

IPC分类号： C02F1/40

CPC分类号： G01N27/44721 ,  G01N27/44782

摘要： A capillary array includes a light detection portion, a sample supply portion, a buffer solution supply portion and a voltage application portion which are necessary functions for electrophoresis, thereby, when assembling the capillary array into an electrophoresis apparatus, the same can be immediately used. Accordingly, a capillary array is provided which can be easily incorporated into an electrophoresis apparatus.

摘要翻译： 毛细管阵列包括作为电泳所需功能的光检测部分，样品供应部分，缓冲溶液供应部分和电压施加部分，从而当将毛细管阵列组装到电泳装置中时可以立即使用。 因此，提供了可以容易地并入电泳装置中的毛细管阵列。

公开(公告)号：US20080099389A1

公开(公告)日：2008-05-01

申请号：US11877944

申请日：2007-10-24

申请人： Yoshihiro Nagaoka ,  Masahito Ito ,  Daizo Tokinaga ,  Tomohiro Shoji ,  Tomonari Morioka

发明人： Yoshihiro Nagaoka ,  Masahito Ito ,  Daizo Tokinaga ,  Tomohiro Shoji ,  Tomonari Morioka

IPC分类号： B01D15/10

CPC分类号： B01D15/22 ,  B01J2220/82 ,  G01N30/6026 ,  G01N30/6047 ,  G01N30/6052 ,  G01N2030/528

摘要： The flow rate of a solvent is reduced as a separation column and the separation performance is improved even under high-pressure conditions. There is provided a separation column including a monolith rod into which a sample and a mobile phase flow, the separation column comprising: a coating material coated on the outer circumference of a monolith rod; a support member into which the monolith rod coated with the coating material is inserted, and a rod fixing material fitted into or filled a gap between the coating material and the support member; wherein the upper end face of the rod fixing material is sealed, the upper end face being the inflow-side end when the separation column is assembled.

摘要翻译： 溶剂的流量作为分离塔减少，即使在高压条件下也提高了分离性能。 提供了一种分离柱，其包括样品和流动相流入其中的整料棒，所述分离柱包括：涂覆在整料棒外圆周上的涂料; 插入涂覆有涂料的整料棒的支撑构件，以及装配到涂料和支撑构件之间或填充涂层材料和支撑构件之间的间隙的杆固定材料; 其中杆固定材料的上端面被密封，当组装分离柱时，上端面是流入侧端。