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    Production method of oil or fat containing polyunsaturated fatty acid-containing triglyceride
    3.
    发明授权
    Production method of oil or fat containing polyunsaturated fatty acid-containing triglyceride 有权
    含有多不饱和脂肪酸的甘油三酸酯的油脂的生产方法

    公开(公告)号：US07538238B2

    公开(公告)日：2009-05-26

    申请号：US10482373

    申请日：2002-07-02

    申请人： Kengo Akimoto ,  Motoo Sumida ,  Kenichi Higashiyama ,  Shigeaki Fujikawa

    发明人： Kengo Akimoto ,  Motoo Sumida ,  Kenichi Higashiyama ,  Shigeaki Fujikawa

    IPC分类号： A23D9/00

    CPC分类号： C12P7/6472 ,  A23C9/1528 ,  A23C11/04 ,  A23K20/158 ,  A23L33/115 ,  A23L33/12 ,  C11C3/08 ,  C11C3/10

    摘要： The present invention relates to a production method of an oil containing triglyceride in which medium chain fatty acids are bound to the 1- and 3-positions of the triglyceride and polyunsaturated fatty acid is bound to the 2 position by allowing lipase, which specifically acts on ester bonds at the 1- and 3-positions that has been immobilized on a porous ion exchange resin support having a pore size of about 100 Angstroms or more, to act on a mixture of medium-chain fatty acids and raw material oil containing at least one polyunsaturated fatty acid selected from the group consisting of ω6 series polyunsaturated fatty acid having 18 or more carbon atoms and 3 or more double bonds and ω9 series polyunsaturated fatty acid having 18 or more carbon atoms and 2 or more double bonds, but not containing ω3 series polyunsaturated fatty acid, oils and fats or triglycerides obtained by that method, and the use of the oils and fats or triglycerides in a food, beverage or pharmaceutical composition.

    摘要翻译： 本发明涉及含有甘油三酯的油的制备方法，其中中链脂肪酸与甘油三酯的1位和3位结合，多不饱和脂肪酸通过使脂肪酶特异性地作用于2位而结合到2位上 在固定在孔径为约100埃以上的多孔离子交换树脂载体上的1-位和3-位的酯键作用于中链脂肪酸和至少含有至少 一种选自具有18个或更多个碳原子的ω-6系多不饱和脂肪酸和3个或更多个双键的多不饱和脂肪酸和具有18个或更多个碳原子和2个或更多个双键但不含ω3的ω-系列多不饱和脂肪酸 系列多不饱和脂肪酸，通过该方法获得的油和脂肪或甘油三酯，以及在食品，饮料或药物中使用油和脂肪或甘油三酸酯 l组成。

    Process for producing microbial fat or oil having lowered unsaponifiable matter content and said fat or oil
    5.
    发明申请
    Process for producing microbial fat or oil having lowered unsaponifiable matter content and said fat or oil 有权
    用于生产具有降低的不皂化物质含量和所述脂肪或油的微生物脂肪或油的方法

    公开(公告)号：US20050287651A1

    公开(公告)日：2005-12-29

    申请号：US10530747

    申请日：2003-10-09

    申请人： Kengo Akimoto ,  Kenichi Higashiyama ,  Hiroshi Kawashima ,  Motoo Sumida

    发明人： Kengo Akimoto ,  Kenichi Higashiyama ,  Hiroshi Kawashima ,  Motoo Sumida

    IPC分类号： A23D9/02 ,  C12P7/64

    CPC分类号： C12P7/6472 ,  A23D9/02

    摘要： A process for producing a crude oil having lowered unsaponifiable matter content and/or ester-type sterol content and comprising a highly unsaturated fatty acid as a constituent fatty acid, characterized in that a microorganism capable of producing a fat or oil comprising an unsaturated fatty acid as a constituent fatty acid is cultured in a medium containing a nitrogen source concentration of 2 to 15% within a culture tank equipped with an agitation impeller satisfying the requirement that the ratio of the diameter of agitation impeller (=d) to the inner diameter of the culture tank (=D) is d/D=0.30 to 0.6.

    摘要翻译： 一种生产具有降低的不皂化物质含量和/或酯类固醇含量并含有高度不饱和脂肪酸作为构成脂肪酸的原油的方法，其特征在于，能够生产包含不饱和脂肪酸的脂肪或油的微生物 作为构成脂肪酸，在含有浓度为2〜15％的氮源的培养基中培养，在配备有搅拌叶轮的培养槽中，搅拌叶轮满足搅拌叶轮直径（= d）与内径 培养罐（= D）为d / D = 0.30〜0.6。

    Media for culturing microorganisms and process for producing unsaturated fatty acids or lipids containing the same
    6.
    发明授权
    Media for culturing microorganisms and process for producing unsaturated fatty acids or lipids containing the same 有权
    用于培养微生物的培养基和用于生产不饱和脂肪酸或含有它们的脂质的方法

    公开(公告)号：US06746857B2

    公开(公告)日：2004-06-08

    申请号：US09331759

    申请日：1999-06-25

    申请人： Kenichi Higashiyama ,  Toshiaki Yaguchi ,  Kengo Akimoto ,  Sakayu Shimizu

    发明人： Kenichi Higashiyama ,  Toshiaki Yaguchi ,  Kengo Akimoto ,  Sakayu Shimizu

    IPC分类号： C12P764

    CPC分类号： C12P7/6427 ,  C12N1/14 ,  C12P7/6472

    摘要： A process for controlling the mycelial morphology of a microorganism belonging to the genus Mortierella during culturing and a process for producing unsaturated fatty acids and a lipid containing them by using a culture medium for culturing a microorganism in which phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the culture medium are in the range of 5 to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively, characterized in that the microorganism belonging to the genus Mortierella is cultured in a culture medium containing phosphate ions in the range of 5 to 60 mM, potassium ions in the range of 5 to 60 mM, sodium ions in the range of 2 to 50 mM, magnesium ions in the range of 0.5 to 9 mM, and calcium ions in the range of 0.5 to 12 mM, respectively, to produce unsaturated fatty acids and the lipid containing them, and the culture medium for culturing a microorganism having phosphate ions, potassium ions, sodium ions, magnesium ions, and calcium ions in the range of 5 to 60 mM, 5 to 60 mM, 2 to 50 mM, 0.5 to 9 mM, and 0.5 to 12 mM, respectively.

    摘要翻译： 用于在培养期间控制属于被孢霉属属的微生物的菌丝体形态的方法以及通过使用培养基培养不饱和脂肪酸和含有它们的脂质的方法来培养微生物的方法，其中磷酸根离子，钾离子，钠离子， 培养基中的镁离子和钙离子分别为5〜60mM，5〜60mM，2〜50mM，0.5〜9mM，以及0.5〜12mM的范围，其特征在于，属于 将被孢霉属属菌体培养在含有5-60mM磷酸根离子，5-60mM范围钾离子，2-50mM范围内的钠离子，0.5- 至9mM，钙离子分别为0.5-12mM，以产生不饱和脂肪酸和含有它们的脂质，以及用于培养具有磷酸根离子，钾离子，钠离子，镁离子的微生物的培养基 s和钙离子分别在5至60mM，5至60mM，2至50mM，0.5至9mM和0.5至12mM的范围内。

    Edible oil containing arachidonic acid and foods containing the same
    7.
    发明授权
    Edible oil containing arachidonic acid and foods containing the same 有权
    含有花生四烯酸的食用油和含有它的食品

    公开(公告)号：US6117905A

    公开(公告)日：2000-09-12

    申请号：US284205

    申请日：1999-06-29

    申请人： Kenichi Higashiyama ,  Kengo Akimoto ,  Sakayu Shimizu ,  Nobushige Doisaki ,  Kiyomi Furihata

    发明人： Kenichi Higashiyama ,  Kengo Akimoto ,  Sakayu Shimizu ,  Nobushige Doisaki ,  Kiyomi Furihata

    IPC分类号： A23C9/152 ,  A23D9/00 ,  A23D9/007 ,  A23L1/30 ,  C12P7/64 ,  C12R1/645 ,  A61K31/20

    CPC分类号： A23D9/00 ,  A23L33/12 ,  A23L33/40 ,  A23V2002/00

    摘要： Edible oil containing arachidonic acid obtained from microorganisms belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid are provided. The oil contain little unsaponifiable matters and, above all, the smallest possible amount of sterol with cyclopropane structure which have not been recognized as food components, and are suitable for the production of foods, in particular, infant formula.Arachidonic acid-containing edible oil originating in microorganisms containing not more than 0.8% by weight, preferably not more than 0.6% by weight of unsaponifiable matters and 20% by weight or more of arachidonic acid. Further, these edible oil contain not more than 0.3% by weight, preferably not more than 0.15% by weight of 24,25-methylenecholest-5-en-3 .beta.-ol. The microorganisms are those belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid. These microorganisms belong to the species alpine of the genus Mortierella. Foods including the arachidonic acid-containing edible oil. Formula for premature infants, formula for infants, foods for infants, and foods for pregnant women and nursing mothers, including the arachidonic acid-containing edible oil.

    摘要翻译： PCT No.PCT / JP97 / 03631 Sec。 371日期1999年6月29日第 102（e）1999年6月29日PCT PCT 1997年10月9日PCT公布。 公开号WO98 / 16119 日期：1998年04月23日提供了从属于被孢霉属的被孢霉属的属于微生物的微生物获得的能够产生花生四烯酸的花生四烯酸的可食用油。 该油含有少量不皂化物质，最重要的是尽可能少量的具有环丙烷结构的固醇未被认定为食品成分，并且适用于生产食品，特别是婴儿配方奶粉。 来源于不超过0.8重量％，优选不超过0.6重量％的不皂化物和20重量％以上花生四烯酸的微生物的含花生四烯酸的食用油。 此外，这些食用油含有不多于0.3重量％，优选不超过0.15重量％的24,25-亚甲基胆甾-5-烯-3β-醇。 这些微生物属于被孢霉属的被孢霉属亚属属，并且能够产生花生四烯酸。 这些微生物属于被孢霉属的高山属。 食物包括含花生四烯酸的食用油。 婴幼儿配方奶粉，婴儿配方食品，孕妇食品，哺乳母亲，包括含花生四烯酸的食用油。

    Process for producing arachidonic acid-containing lipids
    10.
    发明授权
    Process for producing arachidonic acid-containing lipids 失效
    制备含花生四烯酸的脂质的方法

    公开(公告)号：US07585651B2

    公开(公告)日：2009-09-08

    申请号：US11167224

    申请日：2005-06-28

    申请人： Kengo Akimoto ,  Kenichi Higashiyama ,  Sakayu Shimizu

    发明人： Kengo Akimoto ,  Kenichi Higashiyama ,  Sakayu Shimizu

    IPC分类号： C12P7/64

    CPC分类号： C12P7/6472 ,  Y10S435/911

    摘要： A process for producing lipids containing arachidonic acid comprising culturing a microorganism in which ω3 desaturase activity has been decreased or is lacking at a temperature lower than the optimum growth temperature from the start of culturing or after culturing at the optimum growth temperature, said microorganism being obtained by the mutation treatment of a microorganism capable of producing arachidonic acid and belonging to the genus Mortierella and the like; and then recovering lipids containing arachidonic acid from the culture.

    摘要翻译： 一种含花生四烯酸的脂质的制造方法，其特征在于，在培养开始时或培养后，在最适生长温度下培养微生物，其中ω3去饱和酶活性降低或不足于最佳生长温度的温度，所述微生物获得 通过突变处理能够产生花生四烯酸并属于被孢霉属等的微生物; 然后从培养物中回收含有花生四烯酸的脂质。