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    Hepatitis C virus gene
    1.
    发明授权
    Hepatitis C virus gene 有权
    丙型肝炎病毒基因

    公开(公告)号：US08981073B2

    公开(公告)日：2015-03-17

    申请号：US13823422

    申请日：2011-10-07

    申请人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai

    发明人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai

    IPC分类号： C07H21/04 ,  A01N63/02 ,  C12N7/00 ,  C07H21/02 ,  C07K14/005

    CPC分类号： C07H21/02 ,  C07K14/005 ,  C12N2770/24222

    摘要： Disclosed are an HCV gene having higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b, an RNA replicon having this gene, a cell infected with this RNA replicon, which cell allows replication of HCV, and an HCV particle. The hepatitis C virus gene encodes an amino acid sequence wherein the 979th amino acid is threonine; the 1804th amino acid is leucine; and the 1966th amino acid is lysine. An HCV gene which can propagate in vitro and has higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b was provided.

    摘要翻译： 公开了具有比基因型1b的已知HCV基因，具有该基因的RNA复制子，感染了该RNA复制子的细胞，该细胞允许HCV复制和HCV颗粒更高的复制效率和更高的再感染效率的HCV基因。 丙型肝炎病毒基因编码氨基酸序列，其中第979位氨基酸是苏氨酸; 第1804位氨基酸为亮氨酸; 而第1966位氨基酸是赖氨酸。 提供了可以在体外繁殖并且具有比已知的基因型1b的HCV基因更高的复制效率和更高的再感染效率的HCV基因。

    HCV/GBV-B chimeric virus
    2.
    发明授权
    HCV/GBV-B chimeric virus 失效
    HCV / GBV-B嵌合病毒

    公开(公告)号：US08741607B2

    公开(公告)日：2014-06-03

    申请号：US13054356

    申请日：2009-07-15

    申请人： Noboru Maki ,  Kenichi Mori ,  Hiromi Fukai

    发明人： Noboru Maki ,  Kenichi Mori ,  Hiromi Fukai

    IPC分类号： C12N15/09 ,  C12N5/10 ,  C12N7/00 ,  C07K14/18

    CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24243

    摘要： An HCV/GBV-B chimeric virus which maintains the replication function of HCV and is capable of infecting tamarin is disclosed in order to construct an HCV animal model which can be used as a development or evaluation system for therapeutic agents for HCV. The HCV/GBV-B chimeric RNA comprises an RNA of hepatitis C virus and an RNA of GB virus-B, wherein the RNA of hepatitis C virus comprises an RNA encoding leucine at the 1804th position and lysine at the 1966th position in the amino acid sequence of the polyprotein of hepatitis C virus.

    摘要翻译： 公开了保持HCV的复制功能并能够感染t am素的HCV / GBV-B嵌合病毒，以构建可用作HCV治疗剂的开发或评估系统的HCV动物模型。 HCV / GBV-B嵌合RNA包含丙型肝炎病毒的RNA和GB病毒B的RNA，其中丙型肝炎病毒的RNA包含编码第1804位亮氨酸的RNA，氨基酸中第1966位的赖氨酸 丙型肝炎病毒多蛋白序列。

    HEPATITIS C VIRUS GENE
    3.
    发明申请
    HEPATITIS C VIRUS GENE 有权
    HEPATITIS C病毒基因

    公开(公告)号：US20130189779A1

    公开(公告)日：2013-07-25

    申请号：US13823422

    申请日：2011-10-07

    申请人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai

    发明人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai

    IPC分类号： C07H21/02

    CPC分类号： C07H21/02 ,  C07K14/005 ,  C12N2770/24222

    摘要： Disclosed are an HCV gene having higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b, an RNA replicon having this gene, a cell infected with this RNA replicon, which cell allows replication of HCV, and an HCV particle. The hepatitis C virus gene encodes an amino acid sequence wherein the 979th amino acid is threonine; the 1804th amino acid is leucine; and the 1966th amino acid is lysine. An HCV gene which can propagate in vitro and has higher replication efficiency and higher reinfection efficiency than the known HCV gene of genotype 1b was provided.

    摘要翻译： 公开了具有比基因型1b的已知HCV基因，具有该基因的RNA复制子，感染了该RNA复制子的细胞，该细胞允许HCV复制和HCV颗粒更高的复制效率和更高的再感染效率的HCV基因。 丙型肝炎病毒基因编码氨基酸序列，其中第979位氨基酸是苏氨酸; 第1804位氨基酸为亮氨酸; 而第1966位氨基酸是赖氨酸。 提供了可以在体外繁殖并且具有比已知的基因型1b的HCV基因更高的复制效率和更高的再感染效率的HCV基因。

    HCV GENE
    4.
    发明申请
    HCV GENE 审中-公开
    HCV基因

    公开(公告)号：US20100173298A1

    公开(公告)日：2010-07-08

    申请号：US12597692

    申请日：2008-04-28

    申请人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai ,  Chiharu Ohue

    发明人： Kenichi Mori ,  Noboru Maki ,  Hiromi Fukai ,  Chiharu Ohue

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C07K14/005 ,  C07H21/02 ,  C12N15/63 ,  C12N5/071 ,  C12N7/00

    CPC分类号： G01N33/5008 ,  C07K14/005 ,  C12N15/86 ,  C12N2770/24222 ,  C12N2770/24243 ,  C12Q1/707 ,  G01N33/5767 ,  G01N2333/186 ,  G01N2500/00

    摘要： The present invention provides a hepatitis C virus gene, a replicon RNA derived from the gene, a replicon-replicating cell into which the replicon RNA is introduced, and a method for screening a drug using the replicon-replicating cell. By introducing a replicon RNA comprising (A) a polynucleotide having the nucleic acid sequence shown in SEQ ID NO: 5; (B) a polynucleotide having the nucleic acid sequence shown in SEQ ID NO: 7; (C) a polynucleotide coding for a polypeptide having the amino acid sequence shown in SEQ ID NO: 6; (D) a polynucleotide coding for a polypeptide having the amino acid sequence shown in SEQ ID NO: 8; or (E) a polynucleotide having a nucleic acid sequence having a homology of not less than 90% with the nucleic acid sequence shown in SEQ ID NO: 5 or SEQ ID NO: 7; or a replicon RNA of the genotype 1b comprising nucleotides coding for the 1804th amino acid leucine and the 1966th amino acid lysine in the amino acid sequence of a hepatitis C virus polyprotein and a polynucleotide coding for an NS4B protein, into a cell, the replicon-replicating cell can be prepared. By using the replicon-replicating cell, the screening for the drug can be carried out.

    摘要翻译： 本发明提供丙型肝炎病毒基因，来源于该基因的复制子RNA，引入复制子RNA的复制子复制细胞和使用复制子复制细胞筛选药物的方法。 通过引入包含（A）具有SEQ ID NO：5所示核酸序列的多核苷酸的复制子RNA; （B）具有SEQ ID NO：7所示核酸序列的多核苷酸; （C）编码具有SEQ ID NO：6所示氨基酸序列的多肽的多核苷酸; （D）编码具有SEQ ID NO：8所示氨基酸序列的多肽的多核苷酸; 或（E）具有与SEQ ID NO：5或SEQ ID NO：7所示核酸序列同源性不低于90％的核酸序列的多核苷酸; 或基因型1b的复制子RNA，其包含编码丙型肝炎病毒多蛋白的氨基酸序列中的第1804位氨基酸亮氨酸和第1966位氨基酸赖氨酸的核苷酸和编码NS4B蛋白的多核苷酸的复制子RNA， 可以制备复制细胞。 通过使用复制子复制细胞，可以进行药物的筛选。

    HCV/GBV-B CHIMERIC VIRUS
    5.
    发明申请
    HCV/GBV-B CHIMERIC VIRUS 失效
    HCV / GBV-B CHIMERIC病毒

    公开(公告)号：US20110289610A1

    公开(公告)日：2011-11-24

    申请号：US13054356

    申请日：2009-07-15

    申请人： Noboru Maki ,  Kenichi Mori ,  Hiromi Fukai

    发明人： Noboru Maki ,  Kenichi Mori ,  Hiromi Fukai

    IPC分类号： A01K67/027 ,  C07K14/18 ,  C12N15/63 ,  C12N5/10 ,  C12N15/51 ,  C12N7/01

    CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24243

    摘要： An HCV/GBV-B chimeric virus which maintains the replication function of HCV and is capable of infecting tamarin is disclosed in order to construct an HCV animal model which can be used as a development or evaluation system for therapeutic agents for HCV. The HCV/GBV-B chimeric RNA comprises an RNA of hepatitis C virus and an RNA of GB virus-B, wherein the RNA of hepatitis C virus comprises an RNA encoding leucine at the 1804th position and lysine at the 1966th position in the amino acid sequence of the polyprotein of hepatitis C virus.

    摘要翻译： 公开了保持HCV的复制功能并能够感染t am素的HCV / GBV-B嵌合病毒，以构建可用作HCV治疗剂的开发或评估系统的HCV动物模型。 HCV / GBV-B嵌合RNA包含丙型肝炎病毒的RNA和GB病毒B的RNA，其中丙型肝炎病毒的RNA包含编码第1804位亮氨酸的RNA，氨基酸中第1966位的赖氨酸 丙型肝炎病毒多蛋白序列。

    Hbv Particles Containing Hbv-Rna
    8.
    发明申请
    Hbv Particles Containing Hbv-Rna 审中-公开
    含有Hbv-Rna的Hbv颗粒

    公开(公告)号：US20070264646A1

    公开(公告)日：2007-11-15

    申请号：US11630254

    申请日：2004-06-22

    申请人： Noboru Maki ,  Tatsuji Kimura ,  Akihiro Matsumoto ,  Akinori Rokuhara ,  Eiji Tanaka ,  Kendo Kiyosawa

    发明人： Noboru Maki ,  Tatsuji Kimura ,  Akihiro Matsumoto ,  Akinori Rokuhara ,  Eiji Tanaka ,  Kendo Kiyosawa

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N7/00

    CPC分类号： C12N7/00 ,  C12N2730/10123 ,  C12Q1/706

    摘要： Hepatitis B virus (HBV) particles that contain HBV-RNA but do not contain HBV-DNA in a body fluid sample; A method of measuring the particles according to claim 1 by measuring HBV-RNA; A method of judging the therapeutic effect of the treatment of HBV infections by a drug having an effect of inhibiting RNA-dependent DNA polymerase, which method comprises performing the measurement on a body fluid of a patient who received said treatment; A method of predicting the risk of developing hepatic carcinoma or selecting risk groups of developing hepatic carcinoma by the above measurement.

    摘要翻译： 在体液样品中含有HBV-RNA但不含有HBV-DNA的乙型肝炎病毒（HBV）颗粒; 一种通过测量HBV-RNA测量根据权利要求1的颗粒的方法; 一种判断通过具有抑制RNA依赖性DNA聚合酶作用的药物治疗HBV感染的治疗效果的方法，该方法包括对接受所述治疗的患者的体液进行测定; 通过上述测量预测肝癌发展风险或选择发展成肝癌风险组的方法。

    Non-a non-b hepatitis-specific antigen and its use in hepatitis diagnosis
    9.
    发明授权
    Non-a non-b hepatitis-specific antigen and its use in hepatitis diagnosis 失效
    非乙型肝炎特异性抗原及其在肝炎诊断中的应用

    公开(公告)号：US5662906A

    公开(公告)日：1997-09-02

    申请号：US449093

    申请日：1995-05-24

    申请人： Noboru Maki ,  Kenjiro Yamaguchi ,  Ayumi Toyoshima ,  Michinori Kohara

    发明人： Noboru Maki ,  Kenjiro Yamaguchi ,  Ayumi Toyoshima ,  Michinori Kohara

    IPC分类号： C07K14/18 ,  C12N15/40 ,  A61K39/29

    CPC分类号： C07K14/005 ,  C12N2770/24222

    摘要： This invention relates to a DNA fragment comprising a base sequence encoding a non-A non-B hepatitis-specific antigen polypeptide, said base sequence being obtained using genetic engineering techniques from non-A non-B hepatitis virus RNA which is isolated directly from blood plasma from non-A non-B hepatitis patients, to an expression vector and a transformant for use in the expression of the DNA fragment, to a single strand DNA sequence for PCR primer, and to use of said polypeptide and said single strand DNA sequence in the detection of the non-A non-B hepatitis virus. The recombinant polypeptide and the single strand DNA sequence for PCR primer make it possible to detect the non-A non-B hepatitis virus with extremely high accuracy.

    摘要翻译： 本发明涉及包含编码非A非乙型肝炎特异性抗原多肽的碱基序列的DNA片段，所述碱基序列使用从直接从血液中分离的非A非乙型肝炎病毒RNA的遗传工程技术获得 来自非A非B型肝炎患者的血浆，用于表达DNA片段的表达载体和转化体，用于PCR引物的单链DNA序列，以及使用所述多肽和所述单链DNA序列 在非A非乙型肝炎病毒的检测中。 用于PCR引物的重组多肽和单链DNA序列使得可以以非常高的精度检测非A非B型肝炎病毒。

    Isolated genes encoding peptidyl prolyl-cis.trans-isomerase and plasmids comprising said genes
    10.
    发明授权
    Isolated genes encoding peptidyl prolyl-cis.trans-isomerase and plasmids comprising said genes 失效
    编码肽基脯氨酰基 - 顺反异构酶的分离基因和包含所述基因的质粒

    公开(公告)号：US5416015A

    公开(公告)日：1995-05-16

    申请号：US89850

    申请日：1993-07-09

    申请人： Toshiya Hayano ,  Setsuko Katou ,  Noboru Maki ,  Nobuhiro Takahashi ,  Masanori Suzuki

    发明人： Toshiya Hayano ,  Setsuko Katou ,  Noboru Maki ,  Nobuhiro Takahashi ,  Masanori Suzuki

    IPC分类号： C12N9/90 ,  C12N15/61 ,  C12N15/63 ,  C12N15/70

    CPC分类号： C12N9/90

    摘要： A yeast PPIase characterized by possessing following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro stands for L-proline), (2) exhibiting a single molecular weight of about 17,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 6.2 in the isoelectric focusing, and (4) inhibited by CsA;an E. coli PPIase-.beta. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 20,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 5.0 in the isoelectric focusing, and (4) no being inhibited by CsA;an E. coli PPIase-.alpha. characterized by possessing the following properties: (1) acting on and isomerizing the bond X.sub.aa -Pro (wherein X.sub.aa stands for any amino acid and Pro for L-proline), (2) exhibiting a single molecular weight of about 22,000 daltons in the sodium dodecyl sulfate-polyacrylamide concentration gradient gel electrophoresis, (3) exhibiting a single isoelectric point of about 9.7 in the isoelectric focusing, and (4) not being inhibited by CsA.

    摘要翻译： 特征在于具有以下性质的酵母PPI酶：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro代表L-脯氨酸），（2）表现出约17,000道尔顿单分子量 在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中，（3）在等电聚焦中表现出约6.2的单一等电点，（4）被CsA抑制; 特征在于具有以下性质的大肠杆菌PPI酶-β：（1）作用于Xaa-Pro（Xaa代表任何氨基酸，Pro-L-脯氨酸，Xaa代表任意氨基酸，Pro表示单分子量） 在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中为约20,000道尔顿，（3）在等电聚焦中呈现约5.0的单等电点，（4）不被CsA抑制; 其特征在于具有以下性质：（1）作用于Xaa-Pro键（Xaa代表任何氨基酸，Pro为L-脯氨酸），（2）表现出单分子量 在十二烷基硫酸钠 - 聚丙烯酰胺浓度梯度凝胶电泳中约为22,000道尔顿，（3）在等电聚焦中显示出约9.7的单一等电点，（4）不被CsA抑制。