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    Nucleotide Sequences Which Code for the tal Gene
    7.
    发明申请
    Nucleotide Sequences Which Code for the tal Gene 有权
    核苷酸序列哪些代码为tal基因

    公开(公告)号：US20110117611A1

    公开(公告)日：2011-05-19

    申请号：US12941608

    申请日：2010-11-08

    申请人： L K. Dunican ,  Ashling McCormack ,  Cliona Stapelton ,  Kevin Burke ,  Bettina Mockel

    发明人： L K. Dunican ,  Ashling McCormack ,  Cliona Stapelton ,  Kevin Burke ,  Bettina Mockel

    IPC分类号： C12P13/04

    CPC分类号： C12N9/0006 ,  C07K2319/00 ,  C12N9/1022 ,  C12P13/06 ,  C12P13/08 ,  C12P13/227

    摘要： The invention relates to an isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequences of SEQ ID NO. 2 or SEQ ID NO. 4, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequences of SEQ ID NO. 2 or SEQ ID NO. 4 c) polynucleotide which is complementary to the polynucleotides of a) or b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c) and a process for the preparation of L-amino acids, which comprises carrying out the following steps: a) fermentation of the desired L-amino acid-producing bacteria in which at least the tal gene is amplified, b) concentration of the desired product in the medium or in the cells of the bacteria and c) isolation of the L-amino acid.

    摘要翻译： 本发明涉及来自棒状细菌的分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含氨基酸序列 SEQ ID NO： 2或SEQ ID NO。 4，b）编码多肽的多核苷酸，其包含与SEQ ID NO：1的氨基酸序列至少70％的程度相同的氨基酸序列。 2或SEQ ID NO。 4）与a）或b）的多核苷酸互补的多核苷酸和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及制备L-氨基酸的方法 其包括进行以下步骤：a）发酵所需的L-氨基酸生产细菌，其中至少有tal基因被扩增，b）所需产物在培养基中或细菌中的浓度，以及 c）分离L-氨基酸。