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    Nucleotide Sequences Which Code for the tal Gene
    4.
    发明申请
    Nucleotide Sequences Which Code for the tal Gene 有权
    核苷酸序列哪些代码为tal基因

    公开(公告)号：US20110117611A1

    公开(公告)日：2011-05-19

    申请号：US12941608

    申请日：2010-11-08

    申请人： L K. Dunican ,  Ashling McCormack ,  Cliona Stapelton ,  Kevin Burke ,  Bettina Mockel

    发明人： L K. Dunican ,  Ashling McCormack ,  Cliona Stapelton ,  Kevin Burke ,  Bettina Mockel

    IPC分类号： C12P13/04

    CPC分类号： C12N9/0006 ,  C07K2319/00 ,  C12N9/1022 ,  C12P13/06 ,  C12P13/08 ,  C12P13/227

    摘要： The invention relates to an isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequences of SEQ ID NO. 2 or SEQ ID NO. 4, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequences of SEQ ID NO. 2 or SEQ ID NO. 4 c) polynucleotide which is complementary to the polynucleotides of a) or b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c) and a process for the preparation of L-amino acids, which comprises carrying out the following steps: a) fermentation of the desired L-amino acid-producing bacteria in which at least the tal gene is amplified, b) concentration of the desired product in the medium or in the cells of the bacteria and c) isolation of the L-amino acid.

    摘要翻译： 本发明涉及来自棒状细菌的分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码多肽的多核苷酸的程度相同的多核苷酸，所述多核苷酸包含氨基酸序列 SEQ ID NO： 2或SEQ ID NO。 4，b）编码多肽的多核苷酸，其包含与SEQ ID NO：1的氨基酸序列至少70％的程度相同的氨基酸序列。 2或SEQ ID NO。 4）与a）或b）的多核苷酸互补的多核苷酸和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及制备L-氨基酸的方法 其包括进行以下步骤：a）发酵所需的L-氨基酸生产细菌，其中至少有tal基因被扩增，b）所需产物在培养基中或细菌中的浓度，以及 c）分离L-氨基酸。

    Process for Preparing L-amino Acids
    10.
    发明申请
    Process for Preparing L-amino Acids 审中-公开
    L-氨基酸的制备方法

    公开(公告)号：US20070122832A1

    公开(公告)日：2007-05-31

    申请号：US11615860

    申请日：2006-12-22

    申请人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    发明人： Bettina Mockel ,  Mike Farwick ,  Thomas Hermann ,  Achim Marx ,  Walter Pfefferle

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P13/08 ,  C12N9/10 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C12N9/1205 ,  C07K14/34 ,  C12N9/10 ,  C12P13/04 ,  C12P13/06 ,  C12P13/08 ,  C12P13/10 ,  C12P13/12 ,  C12P13/14 ,  C12P13/20 ,  C12P13/222 ,  C12P13/225 ,  C12P13/227 ,  C12P13/24

    摘要： The invention relates to isolated polynucleotides comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and a process for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the citA gene is present in attenuated form, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.

    摘要翻译： 本发明涉及分离的多核苷酸，其包含多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码包含SEQ ID No.2的氨基酸序列的多肽的多核苷酸的程度相同至少70％ b）编码多肽的多核苷酸，其包含与SEQ ID No.2的氨基酸序列至少70％相同的氨基酸序列，c）与a）的多核苷酸互补的多核苷酸， 或b），和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及使用棒状细菌发酵制备L-氨基酸的方法，其中至少有citA基因 以减毒形式存在，并且使用包含根据本发明的序列的多核苷酸作为杂交探针。