公开(公告)号：US09034922B2

公开(公告)日：2015-05-19

申请号：US13824325

申请日：2011-09-16

申请人： Koji Eto ,  Ryoko Ohnishi ,  Hiromitsu Nakauchi ,  Takahiko Murata

发明人： Koji Eto ,  Ryoko Ohnishi ,  Hiromitsu Nakauchi ,  Takahiko Murata

IPC分类号： A61K31/27 ,  A01N1/02 ,  A61K31/165 ,  A61K31/18 ,  A61K31/4453 ,  A61K31/5375 ,  C07D295/135 ,  C07C317/28 ,  C12N5/078 ,  A61K39/00

CPC分类号： A01N1/0226 ,  A61K31/165 ,  A61K31/18 ,  A61K31/4453 ,  A61K31/5375 ,  A61K2039/55555 ,  C07C317/28 ,  C07D295/135 ,  C12N5/0644 ,  C12N2501/70 ,  C12N2501/734

摘要： A composition for maintaining a function of platelets, the composition comprising, as an active ingredient, a compound represented by the following general formula (I) or a salt thereof, or a solvate thereof: wherein X represents a phenylene group; Y represents any one of a hydrogen atom and —(CH2)mR1; wherein m represents an integer of any one of 0 to 4; and R1 is any one of —NR5COR2, —NR5SO2R2, and —NR3R4; wherein R2 represents any one of a C1 to C6 alkyl group, an aryl group, a C1 to C6 alkoxy group, and the like; R3 and R4 represent a C1 to C6 alkyl group or the like; and R5 represents any one of a hydrogen atom, a C1 to C6 alkyl group, and the like; and Z represents any one of a hydrogen atom and a C1 to C6 alkyl group.

摘要翻译： 用于维持血小板功能的组合物，所述组合物包含作为活性成分的由以下通式（I）表示的化合物或其盐或其溶剂合物：其中X表示亚苯基; Y表示氢原子和 - （CH 2）m R 1中的任一个; 其中m表示0至4中任一个的整数; 并且R 1是-NR 5 COR 2，-NR 5 SO 2 R 2和-NR 3 R 4中的任一个; 其中R2表示C1至C6烷基，芳基，C1至C6烷氧基等中的任一个; R3和R4表示C1〜C6烷基等; R5表示氢原子，C1〜C6烷基等中的任意一种， Z表示氢原子和C1〜C6烷基中的任一个。

公开(公告)号：US20130177900A1

公开(公告)日：2013-07-11

申请号：US13824325

申请日：2011-09-16

申请人： Koji Eto ,  Ryoko Ohnishi ,  Hiromitsu Nakauchi ,  Takahiko Murata

发明人： Koji Eto ,  Ryoko Ohnishi ,  Hiromitsu Nakauchi ,  Takahiko Murata

IPC分类号： A01N1/02 ,  C07D295/135 ,  C07C317/28

CPC分类号： A01N1/0226 ,  A61K31/165 ,  A61K31/18 ,  A61K31/4453 ,  A61K31/5375 ,  A61K2039/55555 ,  C07C317/28 ,  C07D295/135 ,  C12N5/0644 ,  C12N2501/70 ,  C12N2501/734

摘要： A composition for maintaining a function of platelets, the composition comprising, as an active ingredient, a compound represented by the following general formula (I) or a salt thereof, or a solvate thereof: wherein X represents a phenylene group; Y represents any one of a hydrogen atom and —(CH2)mR1; wherein m represents an integer of any one of 0 to 4; and R1 is any one of —NR5COR2, —NR5SO2R2, and —NR3R4; wherein R2 represents any one of a C1 to C6 alkyl group, an aryl group, a C1 to C6 alkoxy group, and the like; R3 and R4 represent a C1 to C6 alkyl group or the like; and R5 represents any one of a hydrogen atom, a C1 to C6 alkyl group, and the like; and Z represents any one of a hydrogen atom and a C1 to C6 alkyl group.

摘要翻译： 用于维持血小板功能的组合物，所述组合物包含作为活性成分的由以下通式（I）表示的化合物或其盐或其溶剂合物：其中X表示亚苯基; Y表示氢原子和 - （CH 2）m R 1中的任一个; 其中m表示0至4中任一个的整数; 并且R 1是-NR 5 COR 2，-NR 5 SO 2 R 2和-NR 3 R 4中的任一个; 其中R2表示C1至C6烷基，芳基，C1至C6烷氧基等中的任一个; R3和R4表示C1〜C6烷基等; R5表示氢原子，C1〜C6烷基等中的任意一种， Z表示氢原子和C1〜C6烷基中的任一个。

公开(公告)号：US09200254B2

公开(公告)日：2015-12-01

申请号：US13496321

申请日：2010-09-15

申请人： Koji Eto ,  Naoya Takayama ,  Sou Nakamura ,  Hiromitsu Nakauchi

发明人： Koji Eto ,  Naoya Takayama ,  Sou Nakamura ,  Hiromitsu Nakauchi

IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/078 ,  A61K35/00

CPC分类号： C12N5/0641 ,  A61K35/00 ,  C12N5/0644 ,  C12N2501/40 ,  C12N2501/48 ,  C12N2501/60 ,  C12N2501/606 ,  C12N2506/02 ,  C12N2506/45

摘要： The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed.

摘要翻译： 本发明的目的是提供通过在期望的分化阶段中扩增细胞来产生特定细胞的方法。 本发明提供通过诱导细胞分化来产生特异性细胞的方法，其中癌细胞在期望的分化阶段的细胞中被强制表达以扩增所需分化阶段的细胞。 本发明还提供了一种制备特定细胞的方法，其中抑制了在所需分化阶段中在细胞中表达的癌基因诱导的致癌基因诱导的衰老（OIS）。

公开(公告)号：US20140127815A1

公开(公告)日：2014-05-08

申请号：US14117465

申请日：2012-05-11

申请人： Koji Eto ,  Hiromitsu Nakauchi ,  Naoya Takayama ,  Sou Nakamura

发明人： Koji Eto ,  Hiromitsu Nakauchi ,  Naoya Takayama ,  Sou Nakamura

IPC分类号： C12N5/078 ,  C12N15/85

CPC分类号： C12N15/85 ,  A61K35/19 ,  C12N5/0644 ,  C12N2501/165 ,  C12N2501/48 ,  C12N2506/02

摘要： An object of the present invention is to provide a method of promoting polyploidization of megakaryocytes and thereby producing highly polyploidized megakaryocytes, a method of efficiently producing platelets from polyploidized megakaryocytes, and the like. The present invention provides a method of producing polyploidized megakaryocytes comprising a step of forcing expression of an apoptosis suppressor gene in megakaryocytes before polyploidization and culturing the resulting cells.

摘要翻译： 本发明的目的是提供一种促进巨核细胞多倍体化，从而产生高度多倍体的巨核细胞的方法，一种从多倍体巨核细胞中有效产生血小板的方法等。 本发明提供多倍体巨核细胞的制造方法，其特征在于，在多倍体化前，在巨核细胞中强制表达凋亡抑制基因的步骤，培养所得细胞。

公开(公告)号：US20120238023A1

公开(公告)日：2012-09-20

申请号：US13496321

申请日：2010-09-15

申请人： Koji Eto ,  Naoya Takayama ,  Sou Nakamura ,  Hiromitsu Nakauchi

发明人： Koji Eto ,  Naoya Takayama ,  Sou Nakamura ,  Hiromitsu Nakauchi

IPC分类号： C12N15/85 ,  C12N5/10

CPC分类号： C12N5/0641 ,  A61K35/00 ,  C12N5/0644 ,  C12N2501/40 ,  C12N2501/48 ,  C12N2501/60 ,  C12N2501/606 ,  C12N2506/02 ,  C12N2506/45

摘要： The present invention has an object of providing a method for producing specific cells by amplifying cells in a desired differentiation stage. The present invention provides a method for producing specific cells by inducing differentiation of cells, wherein an oncogene is forcibly expressed in cells in a desired differentiation stage to amplify the cells in the desired differentiation stage. The present invention also provides a method for producing specific cells, wherein oncogene-induced senescence (OIS) which is induced by the oncogene expressed in the cells in the desired differentiation stage is suppressed.

摘要翻译： 本发明的目的是提供通过在期望的分化阶段中扩增细胞来产生特定细胞的方法。 本发明提供通过诱导细胞分化来产生特异性细胞的方法，其中癌细胞在期望的分化阶段的细胞中被强制表达以扩增所需分化阶段的细胞。 本发明还提供了一种制备特定细胞的方法，其中抑制了在所需分化阶段中在细胞中表达的癌基因诱导的致癌基因诱导的衰老（OIS）。

公开(公告)号：US08546141B2

公开(公告)日：2013-10-01

申请号：US12935380

申请日：2009-04-01

申请人： Hiromitsu Nakauchi ,  Koji Eto ,  Hidekazu Nishiki-I ,  Naoya Takayama ,  Shinya Yamanaka ,  Kazutoshi Takahashi

发明人： Hiromitsu Nakauchi ,  Koji Eto ,  Hidekazu Nishiki-I ,  Naoya Takayama ,  Shinya Yamanaka ,  Kazutoshi Takahashi

IPC分类号： C12N5/00 ,  C12N15/00

CPC分类号： C12N5/0644 ,  A61K35/12 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/165 ,  C12N2501/91 ,  C12N2502/1394 ,  C12N2506/45

摘要： A method for efficiently preparing blood cells, such as mature megakaryocytes and platelets, from iPS cells is achieved in an in vitro culture system. A sac-like structure encloses hematopoietic progenitor cells, which is obtained by inoculating iPS cells onto feeder cells and then culturing the iPS cells under conditions suitable for inducing the differentiation of hematopoietic progenitor cells. Moreover, a method for producing various types of blood cells, comprises culturing hematopoietic progenitor cells enclosed in the sac-like structure under conditions suitable for inducing the differentiation of blood cells. Furthermore, a method for producing various types of blood cells, particularly megakaryocytes and platelets, is achieved without involving the sac-like structure.

摘要翻译： 在体外培养系统中实现了从iPS细胞中有效地制备血细胞如成熟巨核细胞和血小板的方法。 囊状结构包围造血祖细胞，其通过将iPS细胞接种在饲养细胞上，然后在适于诱导造血祖细胞分化的条件下培养iPS细胞而获得。 此外，生产各种血细胞的方法包括在适于诱导血细胞分化的条件下培养包封在囊样结构中的造血祖细胞。 此外，不涉及囊状结构，可以实现生产各种血细胞，特别是巨核细胞和血小板的方法。

公开(公告)号：US08058064B2

公开(公告)日：2011-11-15

申请号：US12444237

申请日：2004-10-04

申请人： Hiromitsu Nakauchi ,  Koji Eto ,  Naoya Takayama ,  Hidekazu Nishiki-i ,  Hiroko Tsukui

发明人： Hiromitsu Nakauchi ,  Koji Eto ,  Naoya Takayama ,  Hidekazu Nishiki-i ,  Hiroko Tsukui

IPC分类号： C12N5/00

CPC分类号： C12N5/0644 ,  C12N2501/105 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2502/13 ,  C12N2506/02

摘要： An object of the present invention is to provide a sac-like structure enclosing hematopoietic progenitor cells and a method for preparing the sac-like structure as well as a method for efficiently preparing blood cells such as mature megakaryocytes and platelets from the sac-like structure. The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, the sac-like structure being obtained by plating ES cells onto feeder cells and culturing the ES cells under suitable conditions for inducing hematopoietic progenitor cell differentiation. Moreover, the present invention provides a method for producing various blood cells, the method comprising further culturing hematopoietic progenitor cells enclosed in the sac-like structure under suitable conditions for inducing blood cell differentiation.

摘要翻译： 本发明的目的是提供一种封闭造血祖细胞的囊状结构和制备囊状结构的方法以及从囊状结构有效地制备成熟巨核细胞和血小板的血细胞的方法 。 本发明提供封闭造血祖细胞的囊状结构，该囊状结构通过将ES细胞铺在饲养细胞上并在合适的条件下培养ES细胞以诱导造血祖细胞分化而获得。 此外，本发明提供了生产各种血细胞的方法，所述方法还包括在用于诱导血细胞分化的合适条件下包封在囊状结构中的造血祖细胞。

公开(公告)号：US09738906B2

公开(公告)日：2017-08-22

申请号：US14117465

申请日：2012-05-11

申请人： Koji Eto ,  Hiromitsu Nakauchi ,  Naoya Takayama ,  Sou Nakamura

发明人： Koji Eto ,  Hiromitsu Nakauchi ,  Naoya Takayama ,  Sou Nakamura

IPC分类号： C12N5/078 ,  C12N15/85 ,  A61K35/19

CPC分类号： C12N15/85 ,  A61K35/19 ,  C12N5/0644 ,  C12N2501/165 ,  C12N2501/48 ,  C12N2506/02

摘要： An object of the present invention is to provide a method of promoting polyploidization of megakaryocytes and thereby producing highly polyploidized megakaryocytes, a method of efficiently producing platelets from polyploidized megakaryocytes, and the like. The present invention provides a method of producing polyploidized megakaryocytes comprising a step of forcing expression of an apoptosis suppressor gene in megakaryocytes before polyploidization and culturing the resulting cells.

公开(公告)号：US20110053267A1

公开(公告)日：2011-03-03

申请号：US12935380

申请日：2009-04-01

申请人： Hiromitsu Nakauchi ,  Koji Eto ,  Hidekazu Nishiki-I ,  Naoya Takayama ,  Shinya Yamanaka ,  Kazutoshi Takahashi

发明人： Hiromitsu Nakauchi ,  Koji Eto ,  Hidekazu Nishiki-I ,  Naoya Takayama ,  Shinya Yamanaka ,  Kazutoshi Takahashi

IPC分类号： C12N5/078 ,  C12N5/0789

CPC分类号： C12N5/0644 ,  A61K35/12 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/165 ,  C12N2501/91 ,  C12N2502/1394 ,  C12N2506/45

摘要： It is an object of the present invention to provide a method for efficiently preparing blood cells, such as mature megakaryocytes and platelets, from iPS cells in an in vitro culture system.The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, which is obtained by inoculating iPS cells onto feeder cells and then culturing the iPS cells under conditions suitable for inducing the differentiation of hematopoietic progenitor cells. Moreover, the present invention also provides a method for producing various types of blood cells, which comprises culturing hematopoietic progenitor cells enclosed in the sac-like structure under conditions suitable for inducing the differentiation of blood cells. Furthermore, the present invention also provides a method for producing various types of blood cells, particularly megakaryocytes and platelets, without involving the sac-like structure.

摘要翻译： 本发明的目的是在体外培养系统中提供从iPS细胞中有效地制备血细胞如成熟巨核细胞和血小板的方法。 本发明提供封闭造血祖细胞的囊状结构，其通过将iPS细胞接种在饲养细胞上，然后在适于诱导造血祖细胞分化的条件下培养iPS细胞而获得。 此外，本发明还提供了生产各种血细胞的方法，其包括在适于诱导血细胞分化的条件下培养包封在囊状结构中的造血祖细胞。 此外，本发明还提供了不涉及囊状结构来生产各种类型的血细胞，特别是巨核细胞和血小板的方法。

公开(公告)号：US20100197016A1

公开(公告)日：2010-08-05

申请号：US12444237

申请日：2007-10-04

申请人： Hiromitsu Nakauchi ,  Koji Eto ,  Naoya Takayama ,  Hidekazu Nishiki-i ,  Hiroko Tsukui

发明人： Hiromitsu Nakauchi ,  Koji Eto ,  Naoya Takayama ,  Hidekazu Nishiki-i ,  Hiroko Tsukui

IPC分类号： C12N5/078

CPC分类号： C12N5/0644 ,  C12N2501/105 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2502/13 ,  C12N2506/02

摘要： An object of the present invention is to provide a sac-like structure enclosing hematopoietic progenitor cells and a method for preparing the sac-like structure as well as a method for efficiently preparing blood cells such as mature megakaryocytes and platelets from the sac-like structure. The present invention provides a sac-like structure enclosing hematopoietic progenitor cells, the sac-like structure being obtained by plating ES cells onto feeder cells and culturing the ES cells under suitable conditions for inducing hematopoietic progenitor cell differentiation. Moreover, the present invention provides a method for producing various blood cells, the method comprising further culturing hematopoietic progenitor cells enclosed in the sac-like structure under suitable conditions for inducing blood cell differentiation.

摘要翻译： 本发明的目的是提供一种封闭造血祖细胞的囊状结构和制备囊状结构的方法以及从囊状结构有效地制备成熟巨核细胞和血小板的血细胞的方法 。 本发明提供封闭造血祖细胞的囊状结构，该囊状结构通过将ES细胞铺在饲养细胞上并在合适的条件下培养ES细胞以诱导造血祖细胞分化而获得。 此外，本发明提供了生产各种血细胞的方法，所述方法还包括在用于诱导血细胞分化的合适条件下包封在囊状结构中的造血祖细胞。