公开(公告)号：US08486034B2

公开(公告)日：2013-07-16

申请号：US11792114

申请日：2005-12-01

申请人： Kok Kiong Tan ,  Sunan Huang ,  Kok Zuea Tang ,  Soon Chye Ng

发明人： Kok Kiong Tan ,  Sunan Huang ,  Kok Zuea Tang ,  Soon Chye Ng

IPC分类号： A61M1/00

CPC分类号： A61B5/150045 ,  A61B5/150038 ,  A61B5/150068 ,  A61B5/150083 ,  A61B5/150267 ,  A61B17/42 ,  A61M1/02 ,  A61M1/025 ,  A61M2202/0413 ,  A61M2210/1458 ,  A61M2210/1466 ,  A61M2202/0014

摘要： A device for extracting and collecting blood from a delivered placenta comprising a compartment to contain and support a delivered placenta and umbilical cord. The compartment includes a flexible membrane which can be displaced (preferably under the influence of a fluid (preferably gas) pressure differential between the compartment side and non compartment side of the membrane) to impart onto the placenta a pressure to encourage the displacement of fluid carried by the placenta towards the umbilical cord. The compartment includes at least one outlet opening via which the umbilical cord can extend (preferably in a sealed manner) to allow the flexible membrane pressure induced flow of fluid carried by the placenta to displace from the compartment for external to the compartment collection of the fluid.

公开(公告)号：US09494568B2

公开(公告)日：2016-11-15

申请号：US14376279

申请日：2012-02-03

申请人： Soon Chye Ng ,  Swee Lian Liow ,  Naiqing Chen ,  Yen Wah Tong ,  Saif Abdul Kadir Khan ,  Boon Sing Fang

发明人： Soon Chye Ng ,  Swee Lian Liow ,  Naiqing Chen ,  Yen Wah Tong ,  Saif Abdul Kadir Khan ,  Boon Sing Fang

IPC分类号： G01N33/00 ,  C12N5/076 ,  G01N33/483 ,  C12M1/00 ,  B01L3/00 ,  G01N33/487 ,  G01N15/14 ,  C12M3/06 ,  C12N5/071 ,  A61D19/00

CPC分类号： G01N33/4833 ,  A61D19/00 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  C12M23/16 ,  C12M47/04 ,  C12N5/061 ,  C12N5/0612 ,  G01N15/147 ,  G01N33/487 ,  G01N2015/149 ,  Y10T29/49826

摘要： According to embodiments of the present invention, a passive counter flow free microfluidic device for sorting a sample of flagellated cells is provided. The passive counter flow free microfluidic device includes an input reservoir configured to receive the sample of flagellated cells, an output reservoir positioned downstream of the input reservoir, the output reservoir configured to collect the flagellated cells that are sorted, and a plurality of microchannel sections arranged sequentially and in fluid communication with each other between the input reservoir and the output reservoir, and designed to define a closed microchannel system between the input reservoir and the output reservoir, wherein each microchannel section comprises at least one microchannel, wherein an inlet of the microchannel of a first microchannel section of the plurality of microchannel sections is connected to the input reservoir, wherein an outlet of the microchannel of a last microchannel section of the plurality of microchannel sections is connected to the output reservoir, and wherein, for each microchannel section, the total volume of the microchannels of one microchannel section of the plurality of microchannel sections is larger than that of the respective in said downstream direction subsequent microchannel section of the plurality of microchannel sections for sorting the flagellated cells when the flagellated cells move from the input reservoir to the output reservoir.

摘要翻译： 根据本发明的实施例，提供了一种用于对鞭毛细胞样品进行分选的无源逆流微流体装置。 被动无逆流微流体装置包括：输入储存器，其构造成接收鞭毛细胞的样品;位于输入储存器下游的输出储存器，输出储存器，被配置成收集被分类的鞭毛细胞，以及多个微通道部分排列 顺序地并且在输入储存器和输出储存器之间彼此流体连通，并被设计为在输入储存器和输出储存器之间限定封闭的微通道系统，其中每个微通道部分包括至少一个微通道，其中微通道的入口 所述多个微通道部分中的第一微通道部分连接到所述输入储存器，其中所述多个微通道部分中的最后微通道部分的所述微通道的出口连接到所述输出储存器，并且其中，对于每个微通道部分， 微通道的总体积 所述多个微通道部分中的一个微通道部分大于所述多个微通道部分中的所述下游方向的相应的微通道部分，当所述鞭毛状细胞从所述输入储存器移动到所述输出储存器时，用于分选所述鞭毛细胞。

公开(公告)号：US20050063962A1

公开(公告)日：2005-03-24

申请号：US10889225

申请日：2004-07-12

申请人： Soon Chye Ng ,  Naiqing Chen

发明人： Soon Chye Ng ,  Naiqing Chen

IPC分类号： C12N5/075 ,  C12N5/22 ,  C12N15/877 ,  A61K48/00 ,  C12N5/06 ,  C12N5/08

CPC分类号： C12N5/0609 ,  A01K2227/106 ,  C12N15/8776 ,  C12N2500/05 ,  C12N2500/14 ,  C12N2500/34 ,  C12N2500/50 ,  C12N2501/06 ,  C12N2501/999 ,  C12N2510/00 ,  C12N2517/04

摘要： The present invention relates to a method for cell enucleation, comprising removal of the spindle body from a cell in metaphase with a minimal amount of cytoplasm from the cell, said method comprising using polarized light microscopy for visualization of the spindle body during cell enucleation. The present invention also relates to a method for production of an activated reconstructed vertebrate cell, said method comprising: a) culturing a reconstructed vertebrate cell for about 1.5 to about 3 hours after being reconstructed; b) applying at least one electrical pulse to the reconstructed cell; c) culturing the reconstructed cell for about a further 2 hours; and d) treating the reconstructed cell with a chemical activator. The methods of the invention find application in the preparation of totipotent or pluripotent cells of substantially identical genotype, as well as the cloning of vertebrates.

摘要翻译： 本发明涉及一种用于细胞摘除的方法，包括从细胞中以最小量的细胞质从中期的细胞中去除心轴体，所述方法包括使用偏振光显微术在细胞摘除期间可视化心轴体。 本发明还涉及一种生产活化的重建脊椎动物细胞的方法，所述方法包括：a）在重建后，将重建的脊椎动物细胞培养约1.5至约3小时; b）将至少一个电脉冲施加到重建的细胞; c）将重建的细胞再培养2小时; 和d）用化学活化剂处理重建的细胞。 本发明的方法可用于制备基本相同基因型的全能或多能细胞以及脊椎动物的克隆。

公开(公告)号：US20150072374A1

公开(公告)日：2015-03-12

申请号：US14376279

申请日：2012-02-03

申请人： Soon Chye Ng ,  Swee Lian Liow ,  Naiqing Chen ,  Yen Wah Tong ,  Saif Abdul Kadir Khan ,  Boon Sing Fang

发明人： Soon Chye Ng ,  Swee Lian Liow ,  Naiqing Chen ,  Yen Wah Tong ,  Saif Abdul Kadir Khan ,  Boon Sing Fang

IPC分类号： G01N33/483

CPC分类号： G01N33/4833 ,  A61D19/00 ,  B01L3/5027 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/087 ,  C12M23/16 ,  C12M47/04 ,  C12N5/061 ,  C12N5/0612 ,  G01N15/147 ,  G01N33/487 ,  G01N2015/149 ,  Y10T29/49826

摘要： According to embodiments of the present invention, a passive counter flow free microfluidic device for sorting a sample of flagellated cells is provided. The passive counter flow free microfluidic device includes an input reservoir configured to receive the sample of flagellated cells, an output reservoir positioned downstream of the input reservoir, the output reservoir configured to collect the flagellated cells that are sorted, and a plurality of microchannel sections arranged sequentially and in fluid communication with each other between the input reservoir and the output reservoir, and designed to define a closed microchannel system between the input reservoir and the output reservoir, wherein each microchannel section comprises at least one microchannel, wherein an inlet of the microchannel of a first microchannel section of the plurality of microchannel sections is connected to the input reservoir, wherein an outlet of the microchannel of a last microchannel section of the plurality of microchannel sections is connected to the output reservoir, and wherein, for each microchannel section, the total volume of the microchannels of one microchannel section of the plurality of microchannel sections is larger than that of the respective in said downstream direction subsequent microchannel section of the plurality of microchannel sections for sorting the flagellated cells when the flagellated cells move from the input reservoir to the output reservoir.

摘要翻译： 根据本发明的实施例，提供了一种用于对鞭毛细胞样品进行分选的无源逆流微流体装置。 被动无逆流微流体装置包括：输入储存器，其构造成接收鞭毛细胞的样品;位于输入储存器下游的输出储存器，输出储存器，被配置成收集被分类的鞭毛细胞，以及多个微通道部分排列 顺序地并且在输入储存器和输出储存器之间彼此流体连通，并被设计为在输入储存器和输出储存器之间限定封闭的微通道系统，其中每个微通道部分包括至少一个微通道，其中微通道的入口 所述多个微通道部分中的第一微通道部分连接到所述输入储存器，其中所述多个微通道部分中的最后微通道部分的所述微通道的出口连接到所述输出储存器，并且其中，对于每个微通道部分， 微通道的总体积 所述多个微通道部分中的一个微通道部分大于所述多个微通道部分中的所述下游方向的相应的微通道部分，当所述鞭毛状细胞从所述输入储存器移动到所述输出储存器时，用于分选所述鞭毛细胞。

公开(公告)号：US06548544B1

公开(公告)日：2003-04-15

申请号：US09714614

申请日：2000-11-17

申请人： Periannan Adaikan ,  Soon Chye Ng

发明人： Periannan Adaikan ,  Soon Chye Ng

IPC分类号： A61K3120

CPC分类号： A61K31/5575 ,  A61K31/00

摘要： Treatment or prophylaxis of persistent penile erection resulting from, for example, physiological dysfunction, trauma, surgical intervention, or chemical induction. The method of the present invention is practiced by the administration of an agent capable of inducing, promoting, or otherwise facilitating contraction of the cavernosum. Another aspect of the present invention provides a composition comprising a cavernosum contraction-inducing, -promoting, or -facilitating agent. In one embodiment, the agents of the present invention are prostaglandins or functional derivatives, homologous, analogues, agonists, mimetics, or metabolites thereof. This invention also provides a means for diagnosing the physiological basis behind penile dysfunction.

摘要翻译： 治疗或预防由例如生理功能障碍，创伤，外科手术或化学诱导引起的持续性阴茎勃起。 本发明的方法通过施用能诱导，促进或以其它方式促进海绵体收缩的药剂来实施。 本发明的另一方面提供了包含海绵体收缩诱导剂，促进剂或促进剂的组合物。 在一个实施方案中，本发明的药剂是前列腺素或功能衍生物，同源物，类似物，激动剂，模拟物或其代谢物。 本发明还提供了用于诊断阴茎功能障碍背后的生理基础的方法。