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公开(公告)号：US09149569B2
公开(公告)日：2015-10-06
申请号：US13108871
申请日：2011-05-16
申请人： Robert J. Hariri
发明人： Robert J. Hariri
CPC分类号： C12N5/0605 , A01N1/021 , A61K35/12 , A61K35/50 , A61K48/00 , A61K2035/124 , A61M1/02 , A61M2202/0462 , A61M2210/1458 , A61M2210/1466 , C12M29/10 , C12M47/04 , C12N5/0603 , C12N5/0647 , C12N5/0668 , C12N2509/00 , C12N2510/02 , C12N2533/90
摘要： The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.
摘要翻译： 本发明提供了从放血的人胎盘中提取和回收胚胎样干细胞（包括但不限于多潜能或多能干细胞）的方法。 通过灌注放血的胎盘，优选用抗凝血剂溶液来处理胎盘以除去残留的脐带血，以冲洗残留的细胞。 收集来自放血胎盘的残留细胞和灌注液，从残留细胞和灌注液中分离胚胎样干细胞。 本发明还提供了利用分离和灌注的胎盘作为其中传播内源性细胞的生物反应器的方法，其包括但不限于胚胎样干细胞。 本发明还提供了用于在胎盘生物反应器中繁殖外源细胞并从其收集繁殖的外源细胞和生物活性分子的方法。
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公开(公告)号：US08580563B2
公开(公告)日：2013-11-12
申请号：US12259259
申请日：2008-10-27
申请人： Robert J. Hariri
发明人： Robert J. Hariri
CPC分类号： C12N5/0605 , A01N1/021 , A61K35/12 , A61K35/50 , A61K48/00 , A61K2035/124 , A61M1/02 , A61M2202/0462 , A61M2210/1458 , A61M2210/1466 , C12M29/10 , C12M47/04 , C12N5/0603 , C12N5/0647 , C12N5/0668 , C12N2509/00 , C12N2510/02 , C12N2533/90
摘要： The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.
摘要翻译： 本发明提供了从放血的人胎盘中提取和回收胚胎样干细胞（包括但不限于多潜能或多能干细胞）的方法。 通过灌注放血的胎盘，优选用抗凝血剂溶液来处理胎盘以除去残留的脐带血，以冲洗残留的细胞。 收集来自放血胎盘的残留细胞和灌注液，从残留细胞和灌注液中分离胚胎样干细胞。 本发明还提供了利用分离和灌注的胎盘作为其中传播内源性细胞的生物反应器的方法，其包括但不限于胚胎样干细胞。 本发明还提供了用于在胎盘生物反应器中繁殖外源细胞并从其收集繁殖的外源细胞和生物活性分子的方法。
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3.发明授权公开(公告)号：US08486034B2
公开(公告)日：2013-07-16
申请号：US11792114
申请日：2005-12-01
申请人： Kok Kiong Tan , Sunan Huang , Kok Zuea Tang , Soon Chye Ng
发明人： Kok Kiong Tan , Sunan Huang , Kok Zuea Tang , Soon Chye Ng
IPC分类号： A61M1/00
CPC分类号： A61B5/150045 , A61B5/150038 , A61B5/150068 , A61B5/150083 , A61B5/150267 , A61B17/42 , A61M1/02 , A61M1/025 , A61M2202/0413 , A61M2210/1458 , A61M2210/1466 , A61M2202/0014
摘要： A device for extracting and collecting blood from a delivered placenta comprising a compartment to contain and support a delivered placenta and umbilical cord. The compartment includes a flexible membrane which can be displaced (preferably under the influence of a fluid (preferably gas) pressure differential between the compartment side and non compartment side of the membrane) to impart onto the placenta a pressure to encourage the displacement of fluid carried by the placenta towards the umbilical cord. The compartment includes at least one outlet opening via which the umbilical cord can extend (preferably in a sealed manner) to allow the flexible membrane pressure induced flow of fluid carried by the placenta to displace from the compartment for external to the compartment collection of the fluid.
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公开(公告)号：US08293223B2
公开(公告)日：2012-10-23
申请号：US13251059
申请日：2011-09-30
申请人： Robert J. Hariri
发明人： Robert J. Hariri
IPC分类号： A01N63/00
CPC分类号： C12N5/0605 , A01N1/021 , A61K35/12 , A61K35/50 , A61K48/00 , A61K2035/124 , A61M1/02 , A61M2202/0462 , A61M2210/1458 , A61M2210/1466 , C12M29/10 , C12M47/04 , C12N5/0603 , C12N5/0647 , C12N5/0668 , C12N2509/00 , C12N2510/02 , C12N2533/90
摘要： The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.
摘要翻译： 本发明提供了从放血的人胎盘中提取和回收胚胎样干细胞（包括但不限于多潜能或多能干细胞）的方法。 通过灌注放血的胎盘，优选用抗凝血剂溶液来处理胎盘以除去残留的脐带血，以冲洗残留的细胞。 收集来自放血胎盘的残留细胞和灌注液，从残留细胞和灌注液中分离胚胎样干细胞。 本发明还提供了利用分离和灌注的胎盘作为其中传播内源性细胞的生物反应器的方法，其包括但不限于胚胎样干细胞。 本发明还提供了用于在胎盘生物反应器中繁殖外源细胞并从其收集繁殖的外源细胞和生物活性分子的方法。
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5.发明申请PLACENTAL STEM CELLS DERIVE FROM POST-PARTUM MANMALIAN PLACENTA, AND USES AND METHODS OF TREATMENT USING SAID CELLS 审中-公开来自马氏体后代的普通干细胞，以及使用细胞的治疗方法和治疗方法公开(公告)号：US20120034195A1
公开(公告)日：2012-02-09
申请号：US13275246
申请日：2011-10-17
申请人： Robert J. HARIRI
发明人： Robert J. HARIRI
CPC分类号： A61K35/28 , A61K35/50 , A61K35/51 , A61K35/545 , A61K48/00 , A61M1/02 , A61M2202/0462 , A61M2210/1458 , A61M2210/1466 , C12N5/0068 , C12N5/0605 , C12N5/0607 , C12N2500/90 , C12N2509/00 , C12N2533/90
摘要： The present invention provides compositions and methods of using placental stem cells that originate from a post-partum placenta with conventional cord blood compositions or other stem or progenitor cells. The placental stem cells can be used alone or in a mixture with other stem cell populations. In accordance with the present invention, the placental stem cells may be mixed with other stem cell populations, including but not limited to, umbilical cord blood, fetal and neonatal hematopoietic stem cells and progenitor cells, human stem cells and progenitor cells derived from bone marrow. The placental stem cells and the mixed populations of placental stem cells and stem cells have a multitude of uses and applications, including but not limited to, therapeutic uses for transplantation and treatment and prevention of disease, and diagnostic and research uses.
摘要翻译： 本发明提供了使用源自产后胎盘的胎盘干细胞与常规脐带血组合物或其它干细胞或祖细胞的组合物和方法。 胎盘干细胞可以单独使用或与其他干细胞群混合使用。 根据本发明，胎盘干细胞可以与其它干细胞群体混合，包括但不限于脐带血，胎儿和新生儿造血干细胞和祖细胞，源自骨髓的人干细胞和祖细胞 。 胎盘干细胞和胎盘干细胞和干细胞的混合种群具有多种用途和应用，包括但不限于用于移植和治疗和预防疾病的治疗用途以及诊断和研究用途。
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公开(公告)号：US20110223141A1
公开(公告)日：2011-09-15
申请号：US13108891
申请日：2011-05-16
申请人： Robert J. HARIRI
发明人： Robert J. HARIRI
CPC分类号： C12N5/0605 , A01N1/021 , A61K35/12 , A61K35/50 , A61K48/00 , A61K2035/124 , A61M1/02 , A61M2202/0462 , A61M2210/1458 , A61M2210/1466 , C12M29/10 , C12M47/04 , C12N5/0603 , C12N5/0647 , C12N5/0668 , C12N2509/00 , C12N2510/02 , C12N2533/90
摘要： The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.
摘要翻译： 本发明提供了从放血的人胎盘中提取和回收胚胎样干细胞（包括但不限于多潜能或多能干细胞）的方法。 通过灌注放血的胎盘，优选用抗凝血剂溶液来处理胎盘以除去残留的脐带血，以冲洗残留的细胞。 收集来自放血胎盘的残留细胞和灌注液，从残留细胞和灌注液中分离胚胎样干细胞。 本发明还提供了利用分离和灌注的胎盘作为其中传播内源性细胞的生物反应器的方法，其包括但不限于胚胎样干细胞。 本发明还提供了用于在胎盘生物反应器中繁殖外源细胞并从其收集繁殖的外源细胞和生物活性分子的方法。
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7.发明申请公开(公告)号：US20090053805A1
公开(公告)日：2009-02-26
申请号：US12259259
申请日：2008-10-27
申请人： Robert J. HARIRI
发明人： Robert J. HARIRI
IPC分类号： C12N5/06
CPC分类号： C12N5/0605 , A01N1/021 , A61K35/12 , A61K35/50 , A61K48/00 , A61K2035/124 , A61M1/02 , A61M2202/0462 , A61M2210/1458 , A61M2210/1466 , C12M29/10 , C12M47/04 , C12N5/0603 , C12N5/0647 , C12N5/0668 , C12N2509/00 , C12N2510/02 , C12N2533/90
摘要： The present invention provides a method of extracting and recovering embryonic-like stem cells, including, but not limited to pluripotent or multipotent stem cells, from an exsanguinated human placenta. A placenta is treated to remove residual umbilical cord blood by perfusing an exsanguinated placenta, preferably with an anticoagulant solution, to flush out residual cells. The residual cells and perfusion liquid from the exsanguinated placenta are collected, and the embryonic-like stem cells are separated from the residual cells and perfusion liquid. The invention also provides a method of utilizing the isolated and perfused placenta as a bioreactor in which to propagate endogenous cells, including, but not limited to, embryonic-like stem cells. The invention also provides methods for propagation of exogenous cells in a placental bioreactor and collecting the propagated exogenous cells and bioactive molecules therefrom.
摘要翻译： 本发明提供了从放血的人胎盘中提取和回收胚胎样干细胞（包括但不限于多潜能或多能干细胞）的方法。 通过灌注放血的胎盘，优选用抗凝血剂溶液来处理胎盘以除去残留的脐带血，以冲洗残留的细胞。 收集来自放血胎盘的残留细胞和灌注液，从残留细胞和灌注液中分离胚胎样干细胞。 本发明还提供了利用分离和灌注的胎盘作为其中传播内源性细胞的生物反应器的方法，其包括但不限于胚胎样干细胞。 本发明还提供了用于在胎盘生物反应器中繁殖外源细胞并从其收集繁殖的外源细胞和生物活性分子的方法。
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8.发明授权Tissue matrices comprising placental stem cells, and methods of making the same 有权包含胎盘干细胞的组织基质及其制备方法公开(公告)号：US07311904B2
公开(公告)日：2007-12-25
申请号：US10074976
申请日：2002-02-13
申请人： Robert J. Hariri
发明人： Robert J. Hariri
IPC分类号： A01N63/00
CPC分类号： A61K35/50 , A61K35/12 , A61L27/3695 , A61L27/3834 , A61L2430/34 , A61M1/02 , A61M2202/0462 , A61M2210/1458 , A61M2210/1466 , C12N5/0068 , C12N5/0605 , C12N5/0606 , C12N5/0663 , C12N5/0678 , C12N2509/00 , C12N2513/00 , C12N2533/90
摘要： A method of manufacturing a tissue matrix for implantation into a patient is disclosed. The method sets forth collecting embryonic stem cells from a placenta which has been treated to remove residual cord blood and seeding the collected stem cells onto or into a tissue matrix. The seeded tissue matrix is then implanted on or into a patient. The seeded tissue matrix made by the method of the present invention is also disclosed.
摘要翻译： 公开了一种制造用于植入患者的组织基质的方法。 该方法阐述了从胎盘收集胚胎干细胞，其已被处理以除去残留的脐带血，并将收集的干细胞接种到组织基质上或组织基质上。 然后将接种的组织基质植入患者体内或进入患者体内。 还公开了通过本发明的方法制备的接种的组织基质。
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公开(公告)号：US20030036733A1
公开(公告)日：2003-02-20
申请号：US10255294
申请日：2002-09-26
发明人： Lauree Martin , Anne Christian
IPC分类号： A61M005/315
CPC分类号： A61M5/14 , A61M31/00 , A61M37/00 , A61M2210/1466
摘要： An apparatus useful for umbilical cord care and method of its use are disclosed. The apparatus provides an injector that is detachably connected to a disposable applicator. The umbilical cord care apparatus may be provided as a kit, particularly suited for use at home.
摘要翻译： 公开了一种可用于脐带护理的装置及其使用方法。 该装置提供了一个可拆卸地连接到一次性涂抹器的注射器。 脐带护理装置可以作为套件提供，特别适合在家中使用。
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公开(公告)号：US06491682B2
公开(公告)日：2002-12-10
申请号：US09242422
申请日：1999-04-20
申请人： Valter Paderni
发明人： Valter Paderni
IPC分类号： A61M100
CPC分类号： A61M1/02 , A61B5/150038 , A61B5/150045 , A61M2202/0413 , A61M2210/1458 , A61M2210/1466 , A61M2202/0014
摘要： An apparatus (1) for extracting fluids from a body tissue or organ having an aperture, including a seat (26) for positioning said tissue, and a pressure membranes (17,22) for applying pressure to said tissue while leaving said tissue aperture open for the exit of said fluids. The surface (23) of the supporting surface of the tissue is shaped as a funnel or cone, so that the apex is directed downward and the base (29) delimits the supporting surface of the tissue; the apex region being constituted by a hollow cylinder (22) in order to allow the passage of the umbilical cord of a placenta or of the vessel for the drainage of fluids from the placenta or organ or tissue; the supporting surface being provided so as to avoid producing obstructions or collapse of the surface vessels of the placenta or organ or tissue, so as to avoid hindering the flow of blood or fluids
摘要翻译： 一种用于从具有孔的身体组织或器官中提取流体的装置（1），包括用于定位所述组织的座（26）和用于向所述组织施加压力同时留下所述组织孔的压力膜（17,22） 用于所述流体的出口。 组织的支撑表面的表面（23）成形为漏斗或锥体，使得顶点向下指向，并且基部（29）限定组织的支撑表面; 所述顶点区域由中空圆柱体（22）构成，以允许胎盘或所述容器的脐带通过以从所述胎盘或器官或组织排出流体; 提供支撑表面以避免产生胎盘或器官或组织的表面血管的阻塞或塌陷，以避免阻碍血液或流体的流动
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