METHOD OF SEPARATING PANCREATIC ISLET
    2.
    发明申请
    METHOD OF SEPARATING PANCREATIC ISLET 有权
    分离胰岛素的方法

    公开(公告)号:US20130017606A1

    公开(公告)日:2013-01-17

    申请号:US13624309

    申请日:2012-09-21

    IPC分类号: C12N5/071

    摘要: The present invention provides a pancreatic islet isolation method comprising the steps of (1) injecting a protection solution containing a protease inhibitor into the pancreatic duct of an procured pancreas; (3) digesting the pancreas into which the protection solution has been injected; and (4) purifying the digested pancreatic tissue using a purification solution containing a density gradient reagent. The present invention also provides a protection solution for injection into the pancreatic duct, a pancreas preservation solution for the two-layer method, and an islet purification solution.

    摘要翻译: 本发明提供一种胰岛分离方法,其包括以下步骤:(1)将含有蛋白酶抑制剂的保护溶液注入胰腺胰管; (3)消化注入保护液的胰腺; 和(4)使用含有密度梯度试剂的纯化溶液纯化消化的胰腺组织。 本发明还提供了用于注射胰管的保护溶液,用于双层方法的胰脏保存溶液和胰岛净化溶液。