公开(公告)号：US20230249180A1

公开(公告)日：2023-08-10

申请号：US18012653

申请日：2021-11-01

Applicant: LANSION BIOTECHNOLOGY CO., LTD.

Inventor： Xingshang Xu ,  Jeffery Chen

IPC: B01L3/00 ,  B01F33/301 ,  B01F23/451 ,  B01F23/40 ,  B01F35/83

CPC classification number: B01L3/502723 ,  B01F33/3017 ,  B01F23/451 ,  B01F23/483 ,  B01F35/83 ,  B01F2101/23

Abstract: The invention discloses a multifunctional microfluidic detection chip. The detection chip comprises a chip body, on which a sample injection chamber, a sample quantitative chamber, a sample overflow chamber, a diluent storage chamber, a diluent quantitative chamber, a diluent overflow chamber, a quantitative mixing chamber, a reaction chamber and vent holes are disposed; a sample to be detected is injected into the sample injection chamber, and enters the sample quantitative chamber through a microfluidic channel, and the excess reaction sample enters the sample overflow chamber, a diluent in the diluent storage chamber enters the diluent quantitative chamber through a microfluidic channel, and the excess diluent enters the diluent overflow chamber; the reaction chamber includes one or more reaction cavities and a sample blank cavity; after the sample in the sample quantitative chamber is mixed with the diluent in the diluent quantitative chamber uniformly in the quantitative mixing chamber, mixed liquid enters the reaction cavities through microfluidic channels and reacts with a reaction reagent for detection, and enters the sample blank cavity at the same time as a sample blank for detection. The invention can effectively reduce the sample consumption, improve the accuracy of the detection results, and simultaneously detect multiple indicators.

公开(公告)号：US10761092B2

公开(公告)日：2020-09-01

申请号：US16079516

申请日：2017-12-06

Applicant: NANJING LANSION BIOTECHNOLOGY CO., LTD.

Inventor： Xingshang Xu ,  Jeffery Chen ,  Long Wang ,  Weiyan Sun ,  Qilu Wan ,  Shen Yang

IPC: G01N33/558 ,  B01L3/00 ,  F16K99/00 ,  G01N33/543

Abstract: Provided is a multi-flux micro-fluidic chip including a chip body. The chip body includes a fluid inflow cavity communicated with an external air path, reaction-quantification cavities, waste liquid cavities, and a fluid path distribution cavity disposed at a middle position of the chip body. The two or more reaction-quantification cavities are distributed on two sides of the fluid path distribution cavity in rows to form the first and second row of reaction-quantification cavities respectively; and they are communicated with a fluid outlet of the fluid path distribution cavity through fluid path branches, and a fluid inlet of the fluid path distribution cavity through fluid path branches, and a fluid inlet of the fluid path distribution cavity is communicated with a fluid outlet of the fluid inflow cavity and an external fluid path, which making it possible to detect multiple items simultaneously and greatly improving the flux of the micro-fluidic chip.

公开(公告)号：US10648975B2

公开(公告)日：2020-05-12

申请号：US16079440

申请日：2017-12-06

Applicant: NANJING LANSION BIOTECHNOLOGY CO., LTD.

Inventor： Xingshang Xu ,  Jeffery Chen ,  Long Wang ,  Weiyan Sun ,  Qilu Wan ,  Shen Yang

IPC: G01N33/558 ,  B01L3/00 ,  F16K99/00 ,  G01N33/543

Abstract: Provided is a single-channel chemiluminescent micro-fluidic chip, including a chip body with a quantification-reaction cavity and a waste liquid cavity. The quantification-reaction cavity is composed of a quantification-reaction pool on a lower portion and a reaction pool cover plate, the quantification-reaction pool is equally divided into three cavities by two partition plates, a labeled antibody is placed in the middle cavity, coated antibodies are placed in the others, a surface, facing the quantification-reaction pool, of the reaction pool cover plate is equally divided into two parts. In result, the coated antibodies and the labeled antibodies are physically separated to effectively avoid nonspecific binding. Due to a special structure of the reaction tank, wavy fluid flow is generated to fully mix the coated antibodies and the labeled antibodies, which improves testing efficiency and sensitivity.

公开(公告)号：US12128365B2

公开(公告)日：2024-10-29

申请号：US17059473

申请日：2019-04-28

Applicant: LANSION BIOTECHNOLOGY CO., LTD

Inventor： Xingshang Xu ,  Jeffery Chen ,  Pei Yu

IPC: B01D69/12 ,  A61M1/36 ,  B01D61/58 ,  B01D69/02

CPC classification number: B01D69/12 ,  B01D61/58 ,  B01D69/02 ,  A61M1/3635 ,  B01D2323/216 ,  B01D2325/10 ,  B01D2325/36

Abstract: Disclosed are a method for whole blood filtration and a filter membrane structure for whole blood filtration, specifically including the following steps: (1) a filter membrane structure made up of at least two filtration membranes sequentially stacked from top to bottom is selected, and subjected to hemagglutinin treatment for later use; (2) a whole blood sample is added to the filter membrane structure for filtration; and (3) the filtered serum or plasma is collected. The filter membrane structure is composed of at least two filtration membranes stacked from top to bottom, and the pore sizes of the filtration membranes stacked gradually decrease from top to bottom, and the areas of the same gradually increase or are equal to each other from top to bottom.

公开(公告)号：US12055513B2

公开(公告)日：2024-08-06

申请号：US17773045

申请日：2020-12-29

Applicant: LANSION BIOTECHNOLOGY CO., LTD.

Inventor： Xingshang Xu ,  Jeffery Chen

IPC: G01N27/327 ,  B01L3/00 ,  G01N33/543 ,  G01N33/547

CPC classification number: G01N27/3276 ,  B01L3/502707 ,  G01N27/3275 ,  G01N27/3277 ,  G01N27/3278 ,  G01N33/5438 ,  G01N33/547 ,  B01L2300/0645

Abstract: Provided is a preparation method for an immunoelectrode. The immunoelectrode comprises a substrate, a gold layer, a conductive polymer layer and an antibody layer. The substrate, the gold layer, the conductive polymer layer and the antibody layer are sequentially attached from bottom to top. The preparation method for the immunoelectrode specifically comprises the following steps: (1) preparing the conductive polymer layer: preparing a polypyrrole layer on a gold-plated substrate to obtain a polypyrrole/gold-plated substrate; (2) preparing the immunoelectrode: preparing the antibody layer on the polypyrrole layer to obtain an antibody/polypyrrole/gold-plated substrate; and (3) forming an immunoelectrode system: fixing a bare gold-plated substrate to the outer side of the antibody/polypyrrole/gold-plated substrate to obtain the immunoelectrode system. A polypyrrole material is used for fixing an antibody of a biological recognition element and immobilizing the antibody on the immunoelectrode.

公开(公告)号：US12017217B2

公开(公告)日：2024-06-25

申请号：US17435399

申请日：2020-04-13

Applicant: LANSION BIOTECHNOLOGY CO., LTD.

Inventor： Xingshang Xu ,  Jeffery Chen

IPC: B01L3/00 ,  G01N27/327

CPC classification number: B01L3/502715 ,  G01N27/3272 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/141 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2300/0864

Abstract: A multi-channel microfluidic blood coagulation detection chip having a five-layer structure includes a chip body. The chip body includes, in sequence from top to bottom, a first-layer chip, a second-layer chip, a third-layer chip, a fourth-layer chip, and a fifth-layer chip. The first-layer chip (1), the second-layer chip, the third-layer chip, the fourth-layer chip, and the fifth-layer chip cooperate with each other to define a closed microfluidic channel and a plurality of mutually-independent detection chambers. The first-layer chip is provided with a sample loading hole, and the sample loading hole communicates with the detection chambers through the microfluidic channel. The chip body further includes electrodes, and the electrodes are disposed within the detection chambers in one-to-one correspondence.

公开(公告)号：US20240157356A1

公开(公告)日：2024-05-16

申请号：US17915478

申请日：2021-09-27

Applicant: LANSION BIOTECHNOLOGY CO., LTD.

Inventor： Xingshang Xu ,  Jeffery Chen ,  Yabao Chen

IPC: B01L3/00 ,  G01N33/543

CPC classification number: B01L3/502715 ,  G01N33/54386 ,  B01L2200/16 ,  B01L2300/0654 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0677

Abstract: The invention discloses a microfluidic blood type detection chip. The chip comprises a chip body, and the chip body comprises: a forward typing blood type identification area, which is provided with a first sample injection chamber, a quantitative mixing chamber, a forward typing reaction chamber, and a vent hole; a reverse typing blood type identification area, which is provided with a second sample injection chamber, a quantitative separation chamber, a reverse typing reaction chamber, and a vent hole; a sample is injected into the first sample injection chamber, from which the sample and preset diluent flow into the quantitative mixing chamber through a microfluidic channel; after the sample and the diluent are mixed in the quantitative mixing chamber, they enter the forward typing reaction chamber to react with a reaction reagent for detection; a sample is injected into the second sample injection chamber, and enters the quantitative separation chamber through a microfluidic channel, the sample is separated in the quantitative separation chamber, and separated plasma enters the reverse typing reaction chamber and reacts with a reaction reagent for detection. The present invention can realize the simultaneous forward and reverse typing blood type identification, so that the results of blood type identification are more accurate.

公开(公告)号：US11872555B2

公开(公告)日：2024-01-16

申请号：US16973780

申请日：2019-03-21

Applicant: LANSION BIOTECHNOLOGY CO., LTD

Inventor： Xingshang Xu ,  Jeffery Chen

IPC: B01L3/00 ,  G01N33/86 ,  B01L7/00 ,  G01N33/49

CPC classification number: B01L3/502715 ,  B01L7/00 ,  G01N33/4905 ,  G01N33/86 ,  B01L2200/087 ,  B01L2300/023 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/18

Abstract: A detection system for multi-index coagulation items, including a housing, a detection and heating module, a battery module, a touch display screen and a mainboard. The housing includes an upper housing and a lower housing, and is an enclosed space formed by the upper housing abutting the lower housing; the mainboard is disposed between the upper and the lower housing, and is arranged in the enclosed space; the touch display screen is disposed at the upper surface of upper housing, and is connected with the mainboard; the detection and heating module is disposed in the enclosed space, and is arranged at the front end of lower housing for connecting with the mainboard; the battery module is arranged at the bottom of terminal of lower housing, and is connected with the mainboard; and a detection inlet for inserting and placing a detection card is disposed at the front end of housing.

公开(公告)号：US11630113B2

公开(公告)日：2023-04-18

申请号：US17762088

申请日：2021-07-16

Applicant: LANSION BIOTECHNOLOGY CO., LTD.

Inventor： Xingshang Xu ,  Jeffery Chen ,  Peng Wang

IPC: G01N33/53 ,  G01N33/68 ,  G01N33/543

Abstract: A method for assigning an antibody standard and determining a minimum detection limit of an antibody detection reagent specifically includes the following steps: S1, determination of a neutralized antigen equivalent of an antibody standard or a sample: an antigen with a known purity and concentration is subjected to a gradient dilution using a matrix, an equal amount of an antibody standard or antibody-containing sample is added to each of the gradient diluents with different concentrations respectively, and after a reaction, each mixture is subjected to an antibody detection using a first antibody detection reagent, to determine the neutralized antigen equivalent of the antibody standard or sample; S2, determination of an antibody titer of the sample; and S3, acquisition of the minimum detection limit of an antibody detection reagent.

公开(公告)号：US11440006B2

公开(公告)日：2022-09-13

申请号：US16770955

申请日：2019-01-24

Applicant: LANSION BIOTECHNOLOGY CO., LTD

Inventor： Xingshang Xu ,  Jeffery Chen

IPC: B01L3/00

Abstract: A microfluidic detection chip for multi-channel rapid detection, including a chip body. A chip sampling port, a plurality of independent detection chambers, and a microfluidic channel are disposed on the chip body, and the chip sampling port is connected to the detection chambers by means of the microfluidic channel. The chip body further includes an electrode. The detection chambers are connected to the electrode. The microfluidic channel includes a main flow channel and a plurality of branching microfluidic channels. A tail end of the main flow channel is divided into the plurality of branching microfluidic channels, and the plurality of branching microfluidic channels are connected to the plurality of independent detection chambers in a one-to-one corresponding manner. And, the other end of the main flow channel is connected to the chip sampling port.