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公开(公告)号：US20210285037A1
公开(公告)日：2021-09-16
申请号：US17209957
申请日：2021-03-23
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU
IPC: C12Q1/6851 , C12Q1/6806 , C12Q1/686
Abstract: A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13 pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.
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公开(公告)号：US20160251709A1
公开(公告)日：2016-09-01
申请号：US15029088
申请日：2014-09-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q1/6806 , C12Q1/686 , C12Q2561/113 , C12Q2565/607 , C12Q2565/625
Abstract: A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.
Abstract translation: 本文公开了未加工的法医案件样本的直接qPCR定量的工作流程。 已经通过从纸基质的直接扩增检测到13pg的DNA。 未经处理的法医案件样本的直接qPCR定量和在相同PE拭子上收集的未加工的法医案件样本的直接STR扩增将大大增加法医实验室的效率和能力。
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公开(公告)号：US20150111214A1
公开(公告)日：2015-04-23
申请号：US14056921
申请日：2013-10-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU
IPC: C12Q1/68
CPC classification number: C12Q1/6851 , C12Q1/6806 , C12Q1/686 , C12Q2561/113 , C12Q2565/607 , C12Q2565/625
Abstract: A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13 pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability.
Abstract translation: 本文公开了未加工的法医案件样本的直接qPCR定量的工作流程。 已经通过从纸基质直接扩增检测到13pg的DNA。 未经处理的法医案件样本的直接qPCR定量和在相同PE拭子上收集的未加工的法医案件样本的直接STR扩增将大大增加法医实验室的效率和能力。
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公开(公告)号：US20140248626A1
公开(公告)日：2014-09-04
申请号：US14192631
申请日：2014-02-27
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU , Elena Bolchakova , Jaiprakash Shewale , Manohar Furtado
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , C12Q1/6848 , C12Q2523/301 , C12Q2521/301 , C12Q2521/319
Abstract: In general, the disclosed method can be used to remove contaminating microbes and nucleic acids from microorganisms-derived reagents, apparatus and processes (materials and apparatus) related to PCR (and RT-PCR), including sample prep reagents and materials that are used to isolate, purify and detect nucleic acids.
Abstract translation: 通常，所公开的方法可用于从与来自微生物的试剂，与PCR（和RT-PCR）相关的装置和方法（材料和装置））中除去污染微生物和核酸，包括样品制备试剂和用于 分离，纯化和检测核酸。
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公开(公告)号：US20210008548A1
公开(公告)日：2021-01-14
申请号：US16909626
申请日：2020-06-23
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU
IPC: B01L3/00
Abstract: Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.
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Patent Agency Ranking
公开(公告)号：US20170182492A1
公开(公告)日：2017-06-29
申请号：US15125205
申请日：2015-03-16
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU
IPC: B01L3/00
Abstract: Devices, systems, methods, and kits are disclosed for amplifying small quantities of nucleic acid and identifying the nucleic acid sequences of the amplified nucleic acid products.
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公开(公告)号：US20160011085A1
公开(公告)日：2016-01-14
申请号：US14739899
申请日：2015-06-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Michael HARROLD , Lori HENNESSY , Jason Yingjie LIU , Chang ZHONG
CPC classification number: G01N1/30 , A61B5/0077 , A61B5/1172 , A61B5/1411 , A61B5/14514 , A61B5/150022 , A61B5/150083 , A61B5/150091 , A61B5/150412 , A61B5/150984 , A61B5/15105 , A61B5/15134 , A61B5/157 , A61B10/0064 , A61B10/0233 , G06K9/00013 , G06K9/00543 , G06K2009/00946 , G06T7/0012
Abstract: Systems, methods, and kits are disclosed for collection, labeling and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of an individual. Such devices and methods are used in forensic, human identification, access control and screening technologies to rapidly process an individual's identity or determine the identity of an individual.
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公开(公告)号：US20150376721A1
公开(公告)日：2015-12-31
申请号：US14736922
申请日：2015-06-11
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU
CPC classification number: C12Q1/701 , C12Q1/6816 , C12Q1/682 , C12Q1/6851 , C12Q1/686 , C12Q2527/125 , C12Q2531/113 , C12Q2561/113
Abstract: Disclosed herein are methods, compositions and kits for the quantification of a nucleic acid target present on a solid support. This entails quantitative real-time polymerase chain reaction wherein minor groove binders are excluded.
Abstract translation: 本文公开了用于定量存在于固体支持物上的核酸靶的方法，组合物和试剂盒。 这需要定量实时聚合酶链反应，其中排除小沟结合剂。
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公开(公告)号：US20150211051A1
公开(公告)日：2015-07-30
申请号：US14612183
申请日：2015-02-02
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU
IPC: C12Q1/68
CPC classification number: C12Q1/6806 , C12N15/1003
Abstract: A method for selectively recovering nucleic acid from a sperm cell in a sample containing cells of at least a sperm cell and an epithelial cell, and a cell suspension medium comprising extracellular impurities, is provided. The method entails introducing a sample into a vessel, sequestering the cells from the remaining sample components, washing the cells with a washing solution either before or after sequestration, removing the impurities-containing cell suspension medium from the vessel while retaining the cells; lysing selectively cells of the first cell type; and isolating the nucleic acid from the lysed cells. Methods for recovering nucleic acid from the second cell type are also provided.
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公开(公告)号：US20130295572A1
公开(公告)日：2013-11-07
申请号：US13774903
申请日：2013-02-22
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jason Yingjie LIU , Michael HARROLD , Chang ZHONG , Nina PATEL
IPC: G01N1/30
CPC classification number: C12Q1/6806 , A61B10/0045 , A61B10/02 , G01N1/30
Abstract: Systems, methods, and kits are disclosed for collection, labeling and analyzing biological samples containing nucleic acid in conjunction with collecting at least one ridge and valley signature of an individual. Such devices and methods are used in forensic, human identification, access control and screening technologies to rapidly process an individuals identity or determine the identity of an individual.
Abstract translation: 公开了用于收集，标记和分析含有核酸的生物样品以及收集个体的至少一个脊谷签名的系统，方法和试剂盒。 这些设备和方法用于法医，人类识别，访问控制和筛选技术，以快速处理个人身份或确定个人的身份。
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