公开(公告)号：US11235323B2

公开(公告)日：2022-02-01

申请号：US16557192

申请日：2019-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

IPC: B01L1/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00 ,  G01N35/10

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

公开(公告)号：US09808799B2

公开(公告)日：2017-11-07

申请号：US14462640

申请日：2014-08-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

IPC: B01L9/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  G01N35/10

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L7/00 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  G01N2035/00306 ,  G01N2035/1037 ,  Y10T436/2575

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

公开(公告)号：US20150044758A1

公开(公告)日：2015-02-12

申请号：US14462640

申请日：2014-08-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L7/00 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  G01N2035/00306 ,  G01N2035/1037 ,  Y10T436/2575

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

Abstract translation: 本发明提供了用于自动化生物处理的系统，装置，装置和方法。 适用于本发明的方案和生物处理方法的实例包括但不限于免疫沉淀，染色质免疫沉淀，重组蛋白分离，核酸分离和分离，蛋白质标记，分离和分离，细胞分离和分离，食品安全分析和自动珠 基于分离。 在一些实施方案中，本发明提供自动化系统，自动化装置，自动化盒和western印迹处理的自动化方法。 其他实施方案包括自动化系统，自动化装置，自动化盒和用于分离，制备和纯化核酸例如DNA或RNA或其片段的自动化方法，包括质粒DNA，基因组DNA，细菌DNA，病毒DNA和任何其它DNA， 以及用于自动化系统，自动化设备，自动化盒和用于处理，分离和纯化蛋白质，肽等的自动化盒和自动化方法。

公开(公告)号：US20130319863A1

公开(公告)日：2013-12-05

申请号：US13894197

申请日：2013-05-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph Amshey ,  Thomas Diller ,  Regina Rooney

IPC: C07K1/30

CPC classification number: C07K1/306 ,  C30B7/00 ,  C30B29/58

Abstract: The present invention provides methods, compositions, and kits for protein crystallization. The present invention involves electrophoretically focusing at least a first protein species within a matrix comprising at least 2 regions of different pH, the protein being present in amount sufficient to permit crystallization within said pH gradient.

公开(公告)号：US10434509B2

公开(公告)日：2019-10-08

申请号：US15719130

申请日：2017-09-28

Applicant: Life Technologies Corporation

Inventor： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

IPC: G01N1/00 ,  B01L3/00 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00 ,  G01N35/10

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to: immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.