公开(公告)号：US11965201B2

公开(公告)日：2024-04-23

申请号：US17203617

申请日：2021-03-16

Applicant: LanzaTech NZ, Inc.

Inventor： Kaspar Valgepea ,  Michael Koepke ,  James Bruce Yarnton Haycock Behrendorff ,  Esteban Marcellin ,  Lars K. Nielsen ,  Renato de S. P. Lemgruber

IPC: C12P7/56 ,  C12N1/20 ,  C12N1/38 ,  C12N9/06 ,  C12N9/10 ,  C12N9/12 ,  C12N9/78 ,  C12N9/90 ,  C12N15/52 ,  C12P7/04 ,  C12P7/06 ,  C12P7/18 ,  C12P7/40 ,  C12P7/54

CPC classification number: C12P7/56 ,  C12N1/20 ,  C12N1/38 ,  C12N9/0016 ,  C12N9/1018 ,  C12N9/1029 ,  C12N9/1217 ,  C12N9/78 ,  C12N9/90 ,  C12N15/52 ,  C12P7/04 ,  C12P7/065 ,  C12P7/18 ,  C12P7/40 ,  C12P7/54 ,  C12Y104/01012 ,  C12Y201/03003 ,  C12Y207/02002 ,  C12Y305/03006 ,  C12Y501/01012 ,  C12Y504/03005 ,  Y02E50/10

Abstract: The invention provides methods for improving efficiency of fermentation by arginine supplementation, and genetically modified bacterium for use therefor. More particularly the invention provides methods for (i) increasing the production ATP intensive products with arginine supplementation, (ii) increasing utilization of arginine by a C1-fixing bacterium; and (iii) providing C1-fixing bacterium with optimized arginine de-aminase pathways.

公开(公告)号：US11441149B2

公开(公告)日：2022-09-13

申请号：US15442695

申请日：2017-02-26

Applicant: LanzaTech NZ, Inc.

Inventor： Shilpa Nagaraju ,  Michael Koepke

IPC: C12N15/74 ,  C12N15/10

Abstract: The invention provides methods of genetically engineering a C1-fixing bacterium using a Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) (CRISPR/Cas) system. Preferably, the Cas protein is under the control of an inducible promoter.

公开(公告)号：US20230013524A1

公开(公告)日：2023-01-19

申请号：US17823471

申请日：2022-08-30

Applicant: LanzaTech NZ, Inc.

Inventor： Michael Koepke

IPC: C12P5/00 ,  C12N15/74 ,  C12N9/88 ,  C12N9/10 ,  C12N9/12 ,  C12N9/04 ,  C12N15/52 ,  C12P7/42 ,  C12P9/00

Abstract: The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

公开(公告)号：US20230059296A1

公开(公告)日：2023-02-23

申请号：US17934828

申请日：2022-09-23

Applicant: LanzaTech NZ, Inc.

Inventor： Sean Dennis Simpson ,  Christophe Collet ,  Michael Cockrem ,  Simon David Oakley ,  Michael Koepke

IPC: C12P7/28 ,  C12M1/00 ,  C12M1/34 ,  C12P1/04 ,  C12P7/08 ,  C12P7/62 ,  C01B3/38 ,  C12P7/54 ,  C12N1/20 ,  C12P3/00 ,  C12P7/00 ,  C12P7/06

Abstract: What is described is an integrated steel mill and a bioreactor configured to produce useful products from the waste stream of the steel mill. A waste gas stack which is connected to the steel mill is connected to a heat exchanger to cool the waste gas from the steel mill. The cooled gas is pressurized using a pressurization apparatus connected to the heat exchanger. The pressurized gas is sent to an oxygen removal apparatus connected to the pressurization apparatus. An oxygen depleted waste stream from the oxygen removal apparatus is passed to a bioreactor (connected to the oxygen removal apparatus) where microorganisms ferment the waste stream to products. Optional apparatus such as scrubbers, valves, buffers, are also disclosed. The products of the fermentation in the bioreactor can be ethanol and or acetate.

公开(公告)号：US11459589B2

公开(公告)日：2022-10-04

申请号：US17095064

申请日：2020-11-11

Applicant: LanzaTech NZ, Inc.

Inventor： Michael Koepke

IPC: C12P5/00 ,  C12N15/74 ,  C12N9/88 ,  C12N9/10 ,  C12N9/12 ,  C12N9/04 ,  C12N15/52 ,  C12P7/42 ,  C12P9/00

Abstract: The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

公开(公告)号：US12286660B2

公开(公告)日：2025-04-29

申请号：US17823471

申请日：2022-08-30

Applicant: LanzaTech NZ, Inc.

Inventor： Michael Koepke

IPC: C12P5/00 ,  C12N1/21 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52 ,  C12N15/74 ,  C12P7/42 ,  C12P9/00

Abstract: The invention provides a method for producing a terpene or a precursor thereof by microbial fermentation. Typically, the method involves culturing a recombinant bacterium in the presence of a gaseous substrate whereby the bacterium produces a terpene or a precursor thereof, such as mevalonic acid, isopentenyl pyrophosphate, dimethylallyl pyrophosphate, isoprene, geranyl pyrophosphate, farnesyl pyrophosphate, and/or farnesene. The bacterium may comprise one or more exogenous enzymes, such as enzymes in mevalonate, DXS, or terpene biosynthesis pathways.

公开(公告)号：US11549103B2

公开(公告)日：2023-01-10

申请号：US16856841

申请日：2020-04-23

Applicant: LanzaTech NZ, Inc.

Inventor： Shilpa Nagaraju ,  Bakir Al-Sinawi ,  Sashini De Tissera ,  Michael Koepke

IPC: C12P7/06 ,  C12P7/24 ,  C12P13/06 ,  C12N9/04 ,  C12N15/52 ,  C12P7/54 ,  C12P7/18

Abstract: Provided is a method of producing a product by culturing a carboxydotrophic acetogenic bacterium with a disrupting mutation in a lactate dehydrogenase enzyme in the presence of a substrate comprising CO, CO2, and/or H2. Preferably, the disrupting mutation reduces or eliminates the expression or activity of the enzyme such that the bacterium produces a reduced amount of lactate or no lactate.