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公开(公告)号：US20130071898A1
公开(公告)日：2013-03-21
申请号：US13428585
申请日：2012-03-23
申请人： LARRY CAMERON ANTHONY , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica Mcelvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
发明人： LARRY CAMERON ANTHONY , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica Mcelvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
摘要翻译： 本文提供重组酵母宿主细胞及其用于生产异丁醇的方法。 提供的酵母宿主细胞包含异丁醇生物合成途径和降低或消除的醛脱氢酶活性，降低或消除的乙酰乳酸还原酶活性中的至少一种; 或编码具有酮酸酸还原异构酶活性的多肽的异源多核苷酸。
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公开(公告)号：US09790521B2
公开(公告)日：2017-10-17
申请号：US13428585
申请日：2012-03-23
申请人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
发明人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
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3.发明申请KETO-ISOVALERATE DECARBOXYLASE ENZYMES AND METHODS OF USE THEREOF 有权KETO-ISOVALERATE DECARBOXYLASE ENZYMES及其使用方法公开(公告)号：US20130203138A1
公开(公告)日：2013-08-08
申请号：US13560147
申请日：2012-07-27
申请人： Jessica McElvain , Daniel P. Okeefe , Brian James Paul , Mark S. Payne , Steven Cary Rothman , Hongxian He
发明人： Jessica McElvain , Daniel P. Okeefe , Brian James Paul , Mark S. Payne , Steven Cary Rothman , Hongxian He
CPC分类号： C12P7/16 , C12N9/0006 , C12P7/24 , Y02E50/10
摘要： Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.
摘要翻译： 本文提供了编码具有酮异戊酸脱羧酶活性的多肽的多肽和多核苷酸。 还提供了包含这种多肽和多核苷酸的重组宿主细胞及其使用方法。
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公开(公告)号：US20120064585A1
公开(公告)日：2012-03-15
申请号：US13296944
申请日：2011-11-15
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US20100081179A1
公开(公告)日：2010-04-01
申请号：US12569069
申请日：2009-09-29
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US09238828B2
公开(公告)日：2016-01-19
申请号：US13560147
申请日：2012-07-27
申请人： Jessica McElvain , Daniel P O'Keefe , Brian James Paul , Mark S Payne , Steven Cary Rothman , Hongxian He
发明人： Jessica McElvain , Daniel P O'Keefe , Brian James Paul , Mark S Payne , Steven Cary Rothman , Hongxian He
CPC分类号： C12P7/16 , C12N9/0006 , C12P7/24 , Y02E50/10
摘要： Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.
摘要翻译： 本文提供了编码具有酮异戊酸脱羧酶活性的多肽的多肽和多核苷酸。 还提供了包含这种多肽和多核苷酸的重组宿主细胞及其使用方法。
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公开(公告)号：US09206447B2
公开(公告)日：2015-12-08
申请号：US12569069
申请日：2009-09-29
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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8.发明授权Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof 有权具有Fe-S簇蛋白的重组酵母宿主细胞及其使用方法公开(公告)号：US08241878B2
公开(公告)日：2012-08-14
申请号：US13296944
申请日：2011-11-15
申请人： Larry Cameron Anthony , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
发明人： Larry Cameron Anthony , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
IPC分类号： C12P7/58
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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![IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES](/abs-image/US/2010/04/01/US20100081154A1/abs.jpg.150x150.jpg)
9.发明申请IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES 审中-公开细菌[2Fe-2S]二羟基脱氢酶的鉴定和应用公开(公告)号：US20100081154A1
公开(公告)日：2010-04-01
申请号：US12569636
申请日：2009-09-29
CPC分类号： G16B30/00 , C12N9/88 , C12P7/16 , C12P7/40 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.
摘要翻译： 发现一组具有[2Fe-2S]簇的细菌二羟基酸脱水酶。 细菌[2Fe-2S] DHADs在细菌和酵母细胞中表达为异源蛋白质，提供DHAD活性，用于将2,3-二羟基异戊酸酯转化为α-酮异戊酸酯或2,3-二羟甲基戊酸酯转化成α-酮戊二酸酯。 异丁醇和其他化合物可以在包括细菌[2Fe-2S] DHAD活性的途径中合成。
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