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1.发明授权Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof 有权具有Fe-S簇蛋白的重组酵母宿主细胞及其使用方法公开(公告)号：US08241878B2
公开(公告)日：2012-08-14
申请号：US13296944
申请日：2011-11-15
申请人： Larry Cameron Anthony , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
发明人： Larry Cameron Anthony , Dennis Flint , Wonchul Suh , Rick W. Ye , Steven Cary Rothman , Jean-Francois Tomb
IPC分类号： C12P7/58
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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![IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES](/abs-image/US/2010/04/01/US20100081154A1/abs.jpg.150x150.jpg)
2.发明申请IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES 审中-公开细菌[2Fe-2S]二羟基脱氢酶的鉴定和应用公开(公告)号：US20100081154A1
公开(公告)日：2010-04-01
申请号：US12569636
申请日：2009-09-29
CPC分类号： G16B30/00 , C12N9/88 , C12P7/16 , C12P7/40 , C12P13/06 , C12P13/08 , C12Y402/01009 , Y02E50/10
摘要： A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.
摘要翻译： 发现一组具有[2Fe-2S]簇的细菌二羟基酸脱水酶。 细菌[2Fe-2S] DHADs在细菌和酵母细胞中表达为异源蛋白质，提供DHAD活性，用于将2,3-二羟基异戊酸酯转化为α-酮异戊酸酯或2,3-二羟甲基戊酸酯转化成α-酮戊二酸酯。 异丁醇和其他化合物可以在包括细菌[2Fe-2S] DHAD活性的途径中合成。
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3.发明申请FERMENTIVE PRODUCTION OF ISOBUTANOL USING HIGHLY EFFECTIVE KETOL-ACID REDUCTOISOMERASE ENZYMES 审中-公开使用高效的乙醇酸还原酶制剂生产ISOBUTMOL公开(公告)号：US20110244536A1
公开(公告)日：2011-10-06
申请号：US12893077
申请日：2010-09-29
CPC分类号： C12P7/16 , C12N9/0006 , C12N9/88 , C12N15/52 , C12P7/42 , C12Y101/01008 , C12Y101/01086 , C12Y401/01001 , C12Y401/01072 , C12Y401/02009 , C12Y402/01009 , Y02E50/10 , Y02P20/52
摘要： Ketol-acid reductoisomerase enzymes have been identified that provide high effectiveness in vivo as a step in an isobutanol biosynthetic pathway in bacteria and in yeast. These KARIs are members of a clade identified through molecular phylogenetic analysis called the SLSL Clade.
摘要翻译： 已经鉴定了酮醇酸还原异构酶，其在细菌和酵母中作为异丁醇生物合成途径的步骤在体内提供高效力。 这些KARI是通过称为SLSL分子的分子系统发育分析鉴定的进化枝的成员。
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公开(公告)号：US09206447B2
公开(公告)日：2015-12-08
申请号：US12569069
申请日：2009-09-29
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US20120064585A1
公开(公告)日：2012-03-15
申请号：US13296944
申请日：2011-11-15
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US20100081179A1
公开(公告)日：2010-04-01
申请号：US12569069
申请日：2009-09-29
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US09017961B2
公开(公告)日：2015-04-28
申请号：US13412193
申请日：2012-03-05
IPC分类号： C12Q1/34 , C12P7/20 , C07K14/195 , C07K14/24 , C07K14/245 , C07K14/33 , C07K14/335 , C12N9/10 , C12N9/12 , C12N9/26 , C12P7/18 , C12P7/42
CPC分类号： C12P7/20 , C07K14/195 , C07K14/24 , C07K14/245 , C07K14/33 , C07K14/335 , C12N9/1051 , C12N9/1205 , C12N9/2402 , C12N9/2431 , C12P7/18 , C12P7/42 , C12Y204/01007 , C12Y207/01001 , C12Y207/01003 , C12Y207/01004 , C12Y302/01026
摘要： Recombinant bacteria capable of metabolizing sucrose are described. The recombinant bacteria comprise in their genome or on at least one recombinant construct, a novel nucleotide sequence encoding a polypeptide having sucrose transporter activity and a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity. These nucleotide sequences are each operably linked to the same or a different promoter. Recombinant bacteria capable of metabolizing sucrose to produce glycerol and/or glycerol-derived products such as 1,3-propanediol and 3-hydroxypropionic acid are also described.
摘要翻译： 描述了能够代谢蔗糖的重组细菌。 重组细菌在其基因组或至少一种重组构建体中包含编码具有蔗糖转运蛋白活性的多肽的新型核苷酸序列和编码具有蔗糖水解酶活性的多肽的核苷酸序列。 这些核苷酸序列各自可操作地连接到相同或不同的启动子。 还描述了能够代谢蔗糖以产生甘油和/或甘油衍生产物如1,3-丙二醇和3-羟基丙酸的重组细菌。
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8.发明授权Alpha(1,6)-linked glucose oligosaccharide hydrolyzing enzyme polynucleotides and polypeptides and methods of use thereof 有权α（1,6）连接的葡萄糖寡糖水解酶多核苷酸和多肽及其使用方法公开(公告)号：US07268221B2
公开(公告)日：2007-09-11
申请号：US10648152
申请日：2003-08-25
申请人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan Nigarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
发明人： Perry G. Caimi , Mario W. Chen , Vasantha Nagarajan Nigarajan , Jean-Francois Tomb , Siqun Wang , Yuying Zhang
CPC分类号： C12P7/20 , C07K2319/00 , C12N9/2451 , C12N9/246 , C12P7/18 , C12Y302/01068 , C12Y302/01133
摘要： This invention relates to a method for utilizing less purified starch in fermentation processes. One example is a recombinant E. coli containing a exogenous extracellular isoamylase activity that is capable of utilizing small oligomers containing (1,6) linkages (including but not limited to isomaltose and panose) in fermentations to produce useful products. The invention is useful in large-scale industrial biofermentations by reducing the cost of the substrate carbohydrate.
摘要翻译： 本发明涉及一种在发酵过程中利用少量纯化淀粉的方法。 一个实例是含有外源细胞外异淀粉酶活性的重组大肠杆菌，其能够利用在发酵中含有（1,6）键（包括但不限于异麦芽糖和panose）的小寡聚物以产生有用的产物。 通过降低底物碳水化合物的成本，本发明可用于大规模工业生物发酵。
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公开(公告)号：US08962298B2
公开(公告)日：2015-02-24
申请号：US11741916
申请日：2007-04-30
申请人： Gail K. Donaldson , Andrew C. Eliot , Vasantha Nagarajan , Charles E. Nakamura , Jean-Francois Tomb
发明人： Gail K. Donaldson , Andrew C. Eliot , Vasantha Nagarajan , Charles E. Nakamura , Jean-Francois Tomb
IPC分类号： C12N1/20 , C12P7/16 , C12N15/00 , C12N9/00 , C07H21/04 , C12N9/88 , C12N9/04 , C12N9/12 , C12N9/78 , C12P7/26
CPC分类号： C12N9/88 , C12N9/0006 , C12N9/1205 , C12N9/78 , C12P7/16 , C12P7/26 , Y02E50/10
摘要： Methods for the fermentive production of four carbon alcohols are provided. Specifically, butanol, preferably 2-butanol is produced by the fermentive growth of a recombinant bacteria expressing a 2-butanol biosynthetic pathway. The recombinant microorganisms and methods of the invention can also be adapted to produce 2-butanone, an intermediate in the 2-butanol biosynthetic pathways disclosed herein.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达2-丁醇生物合成途径的重组细菌的发酵生长来制备丁醇，优选2-丁醇。 本发明的重组微生物和方法也可适用于生产2-丁酮，本文公开的2-丁醇生物合成途径中的中间体。
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公开(公告)号：US08871488B2
公开(公告)日：2014-10-28
申请号：US13161168
申请日：2011-06-15
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0008 , C12N9/1029 , C12N9/88 , C12N15/52 , C12N15/74 , C12N15/81 , C12P7/18 , C12P7/26 , C12P7/46 , C12P7/56 , C12P13/06 , C12P13/08 , C12Y102/01003 , C12Y102/07001 , C12Y114/15003 , C12Y118/01001 , C12Y118/01002 , C12Y203/01008 , C12Y203/01054 , C12Y401/01001 , C12Y401/02009 , Y02E50/10 , Y02P20/52
摘要： The present invention is related to recombinant host cells comprising: (i) at least one deletion, mutation, and/or substitution in an endogenous gene encoding a polypeptide that converts pyruvate to acetaldehyde, acetyl-phosphate or acetyl-CoA; and (ii) a heterologous polynucleotide encoding a polypeptide having phosphoketolase activity. The present invention is also related to recombinant host cells further comprising (iii) a heterologous polynucleotide encoding a polypeptide having phosphotransacetylase activity.
摘要翻译： 本发明涉及重组宿主细胞，包括：（i）编码将丙酮酸转化为乙醛，乙酰磷酸或乙酰辅酶A的多肽的内源基因中的至少一个缺失，突变和/或取代; 和（ii）编码具有磷酸酮醇酶活性的多肽的异源多核苷酸。 本发明还涉及重组宿主细胞，其还包含（iii）编码具有磷酸转乙酰酶活性的多肽的异源多核苷酸。
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