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    Genes that are Up- or Down-Regulated During Differentiation of Human Embryonic Stem Cells
    2.
    发明申请
    Genes that are Up- or Down-Regulated During Differentiation of Human Embryonic Stem Cells 审中-公开
    在人类胚胎干细胞分化过程中上调或下调的基因

    公开(公告)号：US20090263835A1

    公开(公告)日：2009-10-22

    申请号：US12487869

    申请日：2009-06-19

    申请人： Lawrence W. Stanton ,  Ralph Brandenberger ,  Joseph D. Gold ,  John M. Irving ,  Ramkumar Mandalam ,  Michael Mok ,  Dawne Shelton

    发明人： Lawrence W. Stanton ,  Ralph Brandenberger ,  Joseph D. Gold ,  John M. Irving ,  Ramkumar Mandalam ,  Michael Mok ,  Dawne Shelton

    IPC分类号： G01N33/53

    CPC分类号： C12Q1/6876 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/158

    摘要： Genes that are up- or down-regulated during differentiation provide important leverage by which to characterize and manipulate early-stage pluripotent stem cells. Over 35,000 unique transcripts have been amplified and sequenced from undifferentiated human embryonic stem cells, and three types of differentiated progeny. Statistical analysis of the assembled transcripts identified genes that alter expression levels as differentiation proceeds. The expression profile provides a marker system that has been used to identify particular culture components for maintaining the undifferentiated phenotype. The gene products can also be used to promote differentiation; to assess other relatively undifferentiated cells (such as cancer cells); to control gene expression; or to separate cells having desirable characteristics. Manipulation of particular genes can be used to forestall or focus the differentiation process, en route to producing a specialized homogenous cell population suitable for human therapy.

    摘要翻译： 在分化期间上调或下调的基因提供了表征和操纵早期多能干细胞的重要作用。 已经从未分化的人类胚胎干细胞和三种类型的分化后代中扩增和测序了超过35,000个独特的转录物。 组装的转录物的统计分析确定了在分化进行时改变表达水平的基因。 表达谱提供了用于鉴定用于维持未分化表型的特定培养组分的标记系统。 基因产物也可用于促进分化; 评估其他相对未分化的细胞（如癌细胞）; 控制基因表达; 或分离具有所需特征的细胞。 特定基因的操作可用于预防或集中分化过程，途径是产生适合人类治疗的专门的均质细胞群。

    Marker system for preparing and characterizing high-quality human embryonic stem cells
    3.
    发明授权
    Marker system for preparing and characterizing high-quality human embryonic stem cells 有权
    用于制备和表征高质量人类胚胎干细胞的标记系统

    公开(公告)号：US07153650B2

    公开(公告)日：2006-12-26

    申请号：US10389431

    申请日：2003-03-13

    申请人： Lawrence W. Stanton ,  Ralph Brandenberger ,  Joseph D. Gold ,  John M. Irving ,  Ramkumar Mandalam ,  Michael Mok

    发明人： Lawrence W. Stanton ,  Ralph Brandenberger ,  Joseph D. Gold ,  John M. Irving ,  Ramkumar Mandalam ,  Michael Mok

    IPC分类号： C12Q1/00 ,  C12Q1/68 ,  G01N33/53 ,  C12N5/00 ,  C12N5/02

    CPC分类号： C12Q1/6881 ,  C12Q2600/158 ,  G01N33/5005

    摘要： This disclosure provides a system for qualifying embryonic stem cells intended for human therapy. A large-scale sequencing project has identified important markers that are characteristic of undifferentiated pluripotent cells. Combinations of these markers can be used to validate the self-renewing capacity of ES cells, and their ability to differentiate into tissue types suitable for regenerative medicine. The marker system of this invention has been used to screen feeder cells, media additives, and culture conditions that promote proliferation of stem cells without differentiation. A culture system optimized by following these markers is suitable for rapid expansion of undifferentiated cells from existing lines, or the derivation of new lines that are equally apposite for clinical use.

    摘要翻译： 本公开提供了用于限定用于人类治疗的胚胎干细胞的系统。 大规模测序项目确定了未分化多能细胞特征的重要标志物。 这些标记的组合可以用于验证ES细胞的自我更新能力，以及它们分化成适合再生医学的组织类型的能力。 本发明的标记系统已用于筛选不分化促进干细胞增殖的饲养细胞，培养基添加剂和培养条件。 通过遵循这些标记优化的培养系统适用于从现有品系中未分化细胞的快速扩增，或适用于临床应用的新品系的衍生。

    Synthetic Surfaces for Differentiating Stem Cells into Cardiomyocytes
    8.
    发明申请
    Synthetic Surfaces for Differentiating Stem Cells into Cardiomyocytes 审中-公开
    用于将干细胞分化成心肌细胞的合成表面

    公开(公告)号：US20100248366A1

    公开(公告)日：2010-09-30

    申请号：US12701731

    申请日：2010-02-08

    申请人： Andrei Gennadyevich Fadeev ,  Jennifer Weber ,  Zara Melkoumian ,  David Michael Weber ,  Yue Zhou ,  Ralph Brandenberger

    发明人： Andrei Gennadyevich Fadeev ,  Jennifer Weber ,  Zara Melkoumian ,  David Michael Weber ,  Yue Zhou ,  Ralph Brandenberger

    IPC分类号： C12N5/0735

    CPC分类号： C12N5/0068 ,  C12N5/0606 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2537/10

    摘要： Synthetic surfaces capable of supporting culture of eukaryotic cells including stem cells and undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a polypeptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes a carboxyl group-containing (meth)acrylate monomer, a cross-linking (di- or higher-functional) (meth)acrylate monomer, and a hydrophilic monomer capable of polymerizing with the carboxyl group-containing (meth)acrylate monomer and the cross-linking (meth)acrylate monomer. The swellable (meth)acrylate layer has an equilibrium water content in water of between about 5% and about 70%. The conjugated peptide may include an RGD amino acid sequence.

    摘要翻译： 能够支持培养真核细胞（包括干细胞和未分化的人类胚胎干细胞）的合成表面包括可溶胀的（甲基）丙烯酸酯层和与可溶胀的（甲基）丙烯酸酯层共轭的多肽。 可溶胀的（甲基）丙烯酸酯层可以通过将包含含羧基的（甲基）丙烯酸酯单体，交联（二官能或更高官能）（甲基）丙烯酸酯单体和亲水性（甲基）丙烯酸酯单体）的组合物中的单体聚合而形成 能够与含羧基（甲基）丙烯酸酯单体和交联（甲基）丙烯酸酯单体聚合的单体。 可溶胀的（甲基）丙烯酸酯层在水中的平衡水含量为约5％至约70％。 缀合的肽可以包括RGD氨基酸序列。