公开(公告)号：US20160186166A1

公开(公告)日：2016-06-30

申请号：US14964060

申请日：2015-12-09

Applicant: Life Technologies Corporation ,  Thermo Fisher Scientific Geneart Gmbh ,  Life Technologies As

Inventor： Thomas POEHMERER ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson ,  Xiquan Liang

IPC: C12N15/10 ,  C25B9/18 ,  C25B11/04 ,  C25B15/02 ,  B01L3/00 ,  C25B3/10

CPC classification number: C12N15/101 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00653 ,  B01J2219/00695 ,  B01J2219/00722 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0642 ,  B01L2200/0647 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2400/046 ,  B01L2400/0487 ,  B03C5/026 ,  B03C2201/26 ,  C25B3/10 ,  C25B9/18 ,  C25B11/04 ,  C25B15/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US10407676B2

公开(公告)日：2019-09-10

申请号：US14964060

申请日：2015-12-09

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH ,  LIFE TECHNOLOGIES AS

Inventor： Thomas Poehmerer ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson

IPC: C12N15/10 ,  C25B3/10 ,  C25B11/04 ,  C25B15/02 ,  C25B9/18 ,  B01L3/00 ,  B03C5/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US20230272127A1

公开(公告)日：2023-08-31

申请号：US18143880

申请日：2023-05-05

Applicant: Life Technologies AS

Inventor： Per Nils Pontus Lundberg ,  Geir Fonnum ,  Kristian Andersson

IPC: C08F2/38 ,  C08F228/02 ,  C08F230/02 ,  C08K5/5333 ,  C08L33/26

CPC classification number: C08F2/38 ,  C08F228/02 ,  C08F230/02 ,  C08K5/5333 ,  C08L33/26 ,  C08K3/22

Abstract: This invention also relates to monodisperse coated hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer having a log Poct/wat (log P) of less than about 0.6; and (b) a crosslinker comprising at least two vinyl groups; and a coating. Also provided are methods of forming the monodisperse coated hydrogel polymer particles.

公开(公告)号：US20200010827A1

公开(公告)日：2020-01-09

申请号：US16518099

申请日：2019-07-22

Applicant: THERMO FISHER SCIENTIFIC GENEART GMBH ,  LIFE TECHNOLOGIES AS ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Poehmerer ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson ,  Xiquan Liang

IPC: C12N15/10 ,  C25B3/10 ,  C25B11/04 ,  C25B15/02 ,  C25B9/18 ,  B01L3/00 ,  B03C5/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US11680113B2

公开(公告)日：2023-06-20

申请号：US16478452

申请日：2018-01-19

Applicant: Life Technologies AS

Inventor： Per Nils Pontus Lundberg ,  Geir Fonnum ,  Kristian Andersson

IPC: C08L33/26 ,  C08F2/38 ,  C08F228/02 ,  C08F230/02 ,  C08K5/5333 ,  C08K3/22 ,  C08K5/42

CPC classification number: C08F2/38 ,  C08F228/02 ,  C08F230/02 ,  C08K5/5333 ,  C08L33/26 ,  C08K3/22 ,  C08K5/42 ,  C08K2003/2275 ,  C08K2201/01 ,  C08L2312/00

Abstract: This invention relates to monodisperse magnetic hydrogel polymer particles comprising a magnetic material and a polymer formed from (a) a hydrophilic vinylic monomer having a log Poct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups. The invention also relates to monodisperse coated hydrogel polymer particles comprising a polymer formed from (a) a hydrophilic vinylic monomer having a log Poct/wat (log P) of less than about 0.5; and (b) a crosslinker comprising at least two vinyl groups; and a coating. Also provided are methods of forming the monodisperse magnetic hydrogel polymer particles and monodisperse coated polymer particles.

公开(公告)号：US11041068B2

公开(公告)日：2021-06-22

申请号：US16305372

申请日：2017-06-07

Applicant: LIFE TECHNOLOGIES AS ,  THERMO FISHER SCIENTIFIC GENEART GMBH

Inventor： Kristian Andersson ,  Geir Fonnum ,  Korbinian Heil

IPC: C08L25/14 ,  C08L25/08 ,  B01J19/00 ,  C08F212/08 ,  C08F220/58 ,  C08F293/00

Abstract: This invention relates to polymer particles for solid phase oligonucleotide synthesis. The oligonucleotide may be linked to the particle via a linker having an amide-oligoethyleneglycol-amine structure. The particles may be considered to act as a solid support during the oligonucleotide synthesis. Also disclosed are processes for preparing such polymer particles, compositions and systems comprising such particles, and uses thereof.