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    4,7-Dichlororhodamine dyes
    1.
    发明授权
    4,7-Dichlororhodamine dyes 失效
    4,7-二氯罗丹明染料

    公开(公告)号：US6025505A

    公开(公告)日：2000-02-15

    申请号：US38191

    申请日：1998-03-10

    申请人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    发明人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    IPC分类号： C12N15/09 ,  C07D311/80 ,  C07D311/82 ,  C07D405/12 ,  C07D491/22 ,  C07F9/6561 ,  C07H19/06 ,  C07H19/14 ,  C07H19/16 ,  C07H21/00 ,  C07H21/04 ,  C09B11/24 ,  C09B11/28 ,  C09K11/06 ,  C09K11/07 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/78 ,  G01N27/447 ,  G01N33/58 ,  C07D311/78 ,  C07D311/88

    CPC分类号： C07H21/00 ,  C09B11/24

    摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structure ##STR1## wherein R.sub.1 -R.sub.6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R.sub.1 and R.sub.6 is benzo, or, when taken together, R.sub.4 and R.sub.5 is benzo; Y.sub.1 -Y.sub.4 are hydrogen or lower alkyl, or, when taken together, Y.sub.1 and R.sub.2, Y.sub.2 and R.sub.1 Y.sub.3 and R.sub.3, or Y.sub.4 and R.sub.4 is propano, ethano, or substituted forms thereof, and X.sub.1 -X.sub.3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, --CH.sub.2 OH, and linking group. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其结构是其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸酯，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团，或其组合，或者当R 1和R 6一起时为苯并，或者一起使用时，R 4和R 5为苯并; Y1-Y4是氢或低级烷基，或者当Y1和R2，Y2和R1Y3和R3，或Y4和R4是它们的丙酰，乙酸或取代形式时，分别选择的X1-X3选自 由氢，氯，氟，低级烷基，羧酸酯，磺酸，-CH 2 OH和连接基组成的组。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4,7-dichlororhodamine dyes
    2.
    发明授权
    4,7-dichlororhodamine dyes 有权
    4,7-二氯罗丹明染料

    公开(公告)号：US6017712A

    公开(公告)日：2000-01-25

    申请号：US191421

    申请日：1998-11-12

    申请人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    发明人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    IPC分类号： C07H21/00 ,  C07D311/88 ,  C12Q1/68 ,  C07H19/00

    CPC分类号： C07H21/00

    摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structure ##STR1## wherein R.sub.1 -R.sub.6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R.sub.1 and R.sub.6 is benzo, or, when taken together, R.sub.4 and R.sub.5 is benzo; Y.sub.1 -Y.sub.4 are hydrogen or lower alkyl, or, when taken together, Y.sub.1 and R.sub.2, Y.sub.2 and R.sub.1 Y.sub.3 and R.sub.3, or Y.sub.4 and R.sub.4 is propano, ethano, or substituted forms thereof; and X.sub.1 -X.sub.3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, --CH.sub.2 OH, and linking group. In another aspect, the invention includes reagents labeled with the 4,7-chlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其结构是其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸酯，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团，或其组合，或者当R 1和R 6一起时为苯并，或者一起使用时，R 4和R 5为苯并; Y1-Y4是氢或低级烷基，或者当Y1和R2，Y2和R1Y3和R3，或Y4和R4是丙酰基，乙酰基或取代的形式时; 和分别选择的X1-X3选自氢，氯，氟，低级烷基，羧酸酯，磺酸，-CH 2 OH和连接基团。 另一方面，本发明包括用4,7-氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4,7-dichlororhodamine dyes labeled polynucleotides
    3.
    发明授权
    4,7-dichlororhodamine dyes labeled polynucleotides 失效
    4,7-二氯罗丹明染料标记的多核苷酸

    公开(公告)号：US07550570B2

    公开(公告)日：2009-06-23

    申请号：US10788660

    申请日：2004-02-26

    申请人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Ronald J. Graham

    发明人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Ronald J. Graham

    IPC分类号： C07G3/00 ,  C07H19/04 ,  C12Q1/68

    CPC分类号： C07D401/06 ,  C07D401/14 ,  C07D405/14 ,  C07D491/147 ,  C12Q1/6869 ,  C12Q2563/107 ,  C12Q2535/101

    摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; R7-R10, R12-R16 and R18 may be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11 and R17 may be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4 are hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Y1 and R2, Y2 and R1 Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof; and X1-X3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R1-R6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团时，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; R7-R10，R12-R16和R18可以是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸盐，砜，氨基，酰氨基，腈，低级烷氧基，连接基团; R11和R17可以是氢，低级烷基，低级烯，低级炔，苯基，芳基，连接基团; Y1-Y4是氢，低级烷基或环烷基，或者当一起为Y1和R2，Y2和R1Y3和R3，和/或Y4和R4为丙酰，乙酸或其取代形式时; 单独使用的X 1 -X 3是氢，氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4,7-DICHLORORHODAMINE DYES
    4.
    发明申请
    4,7-DICHLORORHODAMINE DYES 审中-公开
    4,7-二氯丹染色体

    公开(公告)号：US20090093623A1

    公开(公告)日：2009-04-09

    申请号：US12199441

    申请日：2008-08-27

    申请人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Ronald J. Graham

    发明人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Ronald J. Graham

    IPC分类号： C07H21/00 ,  C07H19/10

    CPC分类号： C07D401/06 ,  C07D401/14 ,  C07D405/14 ,  C07D491/147 ,  C12Q1/6869 ,  C12Q2563/107 ,  C12Q2535/101

    摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; R7-R10, R12-R16 and R18 may be hydrogen, fluorine, chlorine, lower alkyl lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11 and R17 may be hydrogen, lower alkyl lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4 are hydrogen, lower alkyl or cycloalkyl, or, when taken together, Y1 and R2, Y2 and R1 Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof, and X1-X3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R1-R6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团时，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; R7-R10，R12-R16和R18可以是氢，氟，氯，低级烷基低级烯，​​低级炔，磺酸，砜，氨基，酰胺基，腈，低级烷氧基，连接基团; R11和R17可以是氢，低级烷基低级烯，​​低级炔，苯基，芳基，连接基; Y1-Y4是氢，低级烷基或环烷基，或者当Y1和R2，Y2和R1Y3和R3和/或Y4和R4分别为丙酰，乙酸或其取代形式时，分别取X1-X3 是氢，氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4,7-dichlororhodamine dyes
    5.
    发明授权
    4,7-dichlororhodamine dyes 有权
    4,7-二氯罗丹明染料

    公开(公告)号：US6080852A

    公开(公告)日：2000-06-27

    申请号：US277793

    申请日：1999-03-27

    申请人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon

    发明人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon

    IPC分类号： G01N33/58 ,  C07D221/12 ,  C07D311/88 ,  C07D491/147 ,  C07F9/6558 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C09B11/24 ,  C09B11/28 ,  C09K11/06 ,  C12Q1/68 ,  C07D309/00 ,  C07D311/78

    CPC分类号： C07H21/00

    摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures ##STR1## wherein R.sub.1 -R.sub.6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrite, lower alkoxy, linking group, or, when taken together, R.sub.1 and R.sub.6 is benzo, or, when taken together, R.sub.4 and R.sub.5 is benzo; R.sub.7 -R.sub.10, R.sub.12 -R.sub.16 and R.sub.18 may be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrite, lower alkoxy, linking group; R.sub.11 and R.sub.17 may be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y.sub.1 -Y.sub.4 are hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Y.sub.1 and R.sub.2, Y.sub.2 and R.sub.1 Y.sub.3 and R.sub.3, and/or Y.sub.4 and R.sub.4 is propano, ethano, or substituted forms thereof, and X.sub.1 -X.sub.3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R1-R6是氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，亚硝酸根，低级烷氧基 或连接基团时，或者当R 1和R 6一起时为苯并，或者合并时，R 4和R 5为苯并; R7-R10，R12-R16和R18可以是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸盐，砜，氨基，酰氨基，亚硝酸盐，低级烷氧基，连接基团; R11和R17可以是氢，低级烷基，低级烯，低级炔，苯基，芳基，连接基团; Y1-Y4是氢，低级烷基或环烷基，或者当Y1和R2，Y2和R1Y3和R3，和/或Y4和R4是共摩尔，乙酸或其取代形式时，并且取X1-X3 分别是氢，氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4, 7-Dichlororhodamine dyes
    6.
    发明授权
    4, 7-Dichlororhodamine dyes 失效
    4，7-二氯罗丹明染料

    公开(公告)号：US5847162A

    公开(公告)日：1998-12-08

    申请号：US672196

    申请日：1996-06-27

    申请人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon

    发明人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon

    IPC分类号： C12N15/09 ,  C07D311/80 ,  C07D311/82 ,  C07D405/12 ,  C07D491/22 ,  C07F9/6561 ,  C07H19/06 ,  C07H19/14 ,  C07H19/16 ,  C07H21/00 ,  C07H21/04 ,  C09B11/24 ,  C09B11/28 ,  C09K11/06 ,  C09K11/07 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/78 ,  G01N27/447 ,  G01N33/58 ,  C07D311/88 ,  C07H19/00

    CPC分类号： C07H21/00 ,  C09B11/24

    摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structure ##STR1## wherein R.sub.1 -R.sub.6 are hydrogen, fluorine, chlorine, lower alkyl lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, lining group, or combinations thereof or, when taken together, R.sub.1 and R.sub.6 is benzo, or, when taken together, R.sub.4 and R.sub.5 is benzo; Y.sub.1 -Y.sub.4 are hydrogen or lower alkyl or, when taken together, Y.sub.1 and R.sub.2 is propano and Y.sub.2 and R.sub.1 is propano, or, when taken together, Y.sub.3 and R.sub.3 is propano and Y.sub.4 and R.sub.4 is propano; and X.sub.1 -X.sub.3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl carboxylate, sulfonic acid, --CH.sub.2 OH, and linking group. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其结构为：其中R 1 -R 6为氢，氟，氯，低级烷基低级烯，​​低级炔，磺酸酯，砜，氨基，酰氨基，腈， 低级烷氧基，内衬基团或其组合，或者当R 1和R 6一起时为苯并，或者当一起时，R 4和R 5为苯并; Y1-Y4是氢或低级烷基，或者一起Y1和R2是丙酰基，Y 2和R 1是丙酰基，或者当Y 3和R 3是丙酰基时，Y 4和R 4是丙酰基; 和分别选择的X1-X3选自氢，氯，氟，低级烷基羧酸酯，磺酸，-CH 2 OH和连接基团。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    Mobility-Modifying Cyanine Dyes
    8.
    发明授权
    Mobility-Modifying Cyanine Dyes 有权
    流动性改性花青染料

    公开(公告)号：US06716994B1

    公开(公告)日：2004-04-06

    申请号：US09477270

    申请日：2000-01-04

    申请人： Steven M. Menchen ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Shaheer H. Khan

    发明人： Steven M. Menchen ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Shaheer H. Khan

    IPC分类号： C07D20902

    CPC分类号： C07H21/00 ,  C07F9/2408 ,  C09B23/02 ,  G01N27/44726

    摘要： The present invention provides a novel class of fluorescent cyanine dye compounds that are modified at one of the hetercyclic ring nitrogen atoms with a mobility-modifying moiety that permits the electrophoretic mobilities of polynucleotides labeled with the mobility-modifying cyanine dyes to be adjusted or tuned in a predictable fashion while retaining enzymatic activity. The ability to predictably tune the relative electrophoretic mobilities of the dyes permits the creation of sets of mobility-matched fluorescent dyes of a variety of structures for a variety of applications, including fluorescence-based 4-color nucleic acid sequencing reactions.

    摘要翻译： 本发明提供了一类新颖的荧光花青染料化合物，其在一个杂环氮原子上被修饰，具有迁移率调节部分，其允许调节或调谐用迁移率调节花青染料标记的多核苷酸的电泳迁移率 一种可预测的方式，同时保留酶活性。 可预测地调节染料的相对电泳迁移率的能力允许为各种应用（包括基于荧光的4色核酸测序反应）创建各种结构的迁移率匹配荧光染料组。