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    4,7-dichlororhodamine dyes
    1.
    发明授权
    4,7-dichlororhodamine dyes 有权
    4,7-二氯罗丹明染料

    公开(公告)号：US6017712A

    公开(公告)日：2000-01-25

    申请号：US191421

    申请日：1998-11-12

    申请人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    发明人： Linda Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    IPC分类号： C07H21/00 ,  C07D311/88 ,  C12Q1/68 ,  C07H19/00

    CPC分类号： C07H21/00

    摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structure ##STR1## wherein R.sub.1 -R.sub.6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R.sub.1 and R.sub.6 is benzo, or, when taken together, R.sub.4 and R.sub.5 is benzo; Y.sub.1 -Y.sub.4 are hydrogen or lower alkyl, or, when taken together, Y.sub.1 and R.sub.2, Y.sub.2 and R.sub.1 Y.sub.3 and R.sub.3, or Y.sub.4 and R.sub.4 is propano, ethano, or substituted forms thereof; and X.sub.1 -X.sub.3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, --CH.sub.2 OH, and linking group. In another aspect, the invention includes reagents labeled with the 4,7-chlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其结构是其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸酯，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团，或其组合，或者当R 1和R 6一起时为苯并，或者一起使用时，R 4和R 5为苯并; Y1-Y4是氢或低级烷基，或者当Y1和R2，Y2和R1Y3和R3，或Y4和R4是丙酰基，乙酰基或取代的形式时; 和分别选择的X1-X3选自氢，氯，氟，低级烷基，羧酸酯，磺酸，-CH 2 OH和连接基团。 另一方面，本发明包括用4,7-氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    4,7-Dichlororhodamine dyes
    2.
    发明授权
    4,7-Dichlororhodamine dyes 失效
    4,7-二氯罗丹明染料

    公开(公告)号：US6025505A

    公开(公告)日：2000-02-15

    申请号：US38191

    申请日：1998-03-10

    申请人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    发明人： Linda G. Lee ,  Scott C. Benson ,  Barnett B. Rosenblum ,  Sandra L. Spurgeon ,  Jonathan M. Cassel ,  Ronald J. Graham

    IPC分类号： C12N15/09 ,  C07D311/80 ,  C07D311/82 ,  C07D405/12 ,  C07D491/22 ,  C07F9/6561 ,  C07H19/06 ,  C07H19/14 ,  C07H19/16 ,  C07H21/00 ,  C07H21/04 ,  C09B11/24 ,  C09B11/28 ,  C09K11/06 ,  C09K11/07 ,  C12P19/34 ,  C12Q1/68 ,  G01N21/78 ,  G01N27/447 ,  G01N33/58 ,  C07D311/78 ,  C07D311/88

    CPC分类号： C07H21/00 ,  C09B11/24

    摘要： A class of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structure ##STR1## wherein R.sub.1 -R.sub.6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or combinations thereof, or, when taken together, R.sub.1 and R.sub.6 is benzo, or, when taken together, R.sub.4 and R.sub.5 is benzo; Y.sub.1 -Y.sub.4 are hydrogen or lower alkyl, or, when taken together, Y.sub.1 and R.sub.2, Y.sub.2 and R.sub.1 Y.sub.3 and R.sub.3, or Y.sub.4 and R.sub.4 is propano, ethano, or substituted forms thereof, and X.sub.1 -X.sub.3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, --CH.sub.2 OH, and linking group. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.

    摘要翻译： 公开了一类用作荧光染料的4,7-二氯罗丹明化合物，其结构是其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯烃，低级炔烃，磺酸酯，砜，氨基，酰氨基，腈，低级烷氧基 或连接基团，或其组合，或者当R 1和R 6一起时为苯并，或者一起使用时，R 4和R 5为苯并; Y1-Y4是氢或低级烷基，或者当Y1和R2，Y2和R1Y3和R3，或Y4和R4是它们的丙酰，乙酸或取代形式时，分别选择的X1-X3选自 由氢，氯，氟，低级烷基，羧酸酯，磺酸，-CH 2 OH和连接基组成的组。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。

    APPARATUS AND METHODS FOR PREPARATION OF SUBTANTIALLY UNIFORM EMULSIONS CONTAINING A PARTICLE
    4.
    发明申请
    APPARATUS AND METHODS FOR PREPARATION OF SUBTANTIALLY UNIFORM EMULSIONS CONTAINING A PARTICLE 审中-公开
    用于制备含有颗粒的亚基均匀乳液的装置和方法

    公开(公告)号：US20090023189A1

    公开(公告)日：2009-01-22

    申请号：US12119475

    申请日：2008-05-12

    申请人： Aldrich N.K. Lau ,  Jonathan M. Cassel ,  John A. Bridgham

    发明人： Aldrich N.K. Lau ,  Jonathan M. Cassel ,  John A. Bridgham

    IPC分类号： C12P19/34 ,  C12M1/40

    CPC分类号： B01F3/0807 ,  B01F5/0475 ,  B01F5/0476 ,  B01F5/0485 ,  B01F13/0059

    摘要： Methods and systems for forming water-in-oil emulsions are described. For example, an apparatus is described which includes: a first compartment containing a plurality of particles dispersed in an aqueous phase; a second compartment containing an oil phase; a porous layer separating the first and second compartments; and a device for applying pressure to the first compartment. A method is described which includes: moving an oil phase relative to a surface of a porous layer while simultaneously forcing an aqueous composition comprising particles through the porous layer and into the flowing dispersion medium thereby forming droplets of the aqueous composition containing particles dispersed in the oil phase. The aqueous composition can include one or more nucleic acid templates and reagents for amplifying the nucleic acids such as PCR reagents. A porous partition is described comprising a first and second major surfaces and at least two straight through pores comprising a cross sectional shape selected from a polygon, an oval, an oblong, a dumbbell, a bowtie and irregular shapes thereof. Aqueous droplets containing an oligonucleotide attached to a particle and reagents can be used as a microreactor for nucleic acid amplification.

    摘要翻译： 描述了用于形成油包水乳液的方法和系统。 例如，描述了一种装置，其包括：包含分散在水相中的多个颗粒的第一隔室; 含有油相的第二隔室; 分离第一和第二隔室的多孔层; 以及用于向第一隔室施加压力的装置。 描述了一种方法，其包括：相对于多孔层的表面移动油相，同时迫使包含颗粒的水性组合物通过多孔层并进入流动分散介质中，由此形成含有分散在油中的颗粒的含水组合物的液滴 相。 水性组合物可以包括用于扩增核酸如PCR试剂的一种或多种核酸模板和试剂。 描述了一种多孔分隔件，其包括第一和第二主表面和至少两个直通孔，其包括选自多边形，椭圆形，长方形，哑铃，弓形和不规则形状的横截面形状。 含有与粒子连接的寡核苷酸的水滴和试剂可用作核酸扩增的微反应器。