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    Stable envelope proteins for retroviral, viral and liposome vectors and use in gene drug therapy
    1.
    发明授权
    Stable envelope proteins for retroviral, viral and liposome vectors and use in gene drug therapy 失效
    用于逆转录病毒，病毒和脂质体载体的稳定的包膜蛋白，用于基因药物治疗

    公开(公告)号：US06448390B1

    公开(公告)日：2002-09-10

    申请号：US09315127

    申请日：1999-05-20

    申请人： Lorraine Moore Albritton ,  Tatiana Zavorotinskaya

    发明人： Lorraine Moore Albritton ,  Tatiana Zavorotinskaya

    IPC分类号： C07H2104

    CPC分类号： C07K14/005 ,  C12N7/00 ,  C12N15/86 ,  C12N15/88 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/13062 ,  C12N2810/50 ,  C12N2810/6054

    摘要： This invention includes retrovirus envelope mutants into which heterologous peptide or glycopeptide sequences can be linked for expression and stable presentation on retroviral vectors. The envelope mutants are characterized by the ability to restore the target penetration capability that is lost or greatly diminished upon fusion of heterologous sequences to the wild type envelope protein and the ability to increase the fusion envelope protein stability and decrease envelope shedding from virus particles. The envelope mutants are created by rotating residues in at least one of 7 motifs. The disclosed envelope proteins also can be used in liposome or pseudotype-virus compositions for delivery of agents including nucleic acid molecules. Methods of preparing and utilizing these envelope mutants in gene therapy are also described.

    摘要翻译： 本发明包括逆转录病毒包膜突变体，异源肽或糖肽序列可以连接到逆转录病毒包膜突变体上，用于在逆转录病毒载体上表达和稳定表达。 信封突变体的特征在于恢复在异源序列融合到野生型包膜蛋白时丢失或大大减少的靶穿透能力的能力，以及增加融合包膜蛋白稳定性并减少病毒颗粒的包膜脱落的能力。 通过在7个图案中的至少一个中旋转残基来产生包膜突变体。 所公开的包膜蛋白也可用于脂质体或假型病毒组合物中用于递送包括核酸分子的试剂。 还描述了在基因治疗中制备和利用这些包膜突变体的方法。