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1.发明申请Method for Determining L-Serine, Gene Sequene, Vectors and Micro-Organisms 审中-公开确定L-丝氨酸，基因序列，载体和微生物的方法公开(公告)号：US20090181435A1
公开(公告)日：2009-07-16
申请号：US12083662
申请日：2006-10-09
摘要： The folic acid concentration in amino acid producing organisms is reduced or completely removed in order to increase the production of L-serine. The enzymes and genes, which are involved in the biosynthesis of folic acid, can be completely excluded or at least reduced in activity. In a preferred embodiment, SEQ ID NOs: 1-7 are utilized.
摘要翻译： 为了增加L-丝氨酸的产生，产生氨基酸的生物体中的叶酸浓度被降低或完全去除。 参与叶酸生物合成的酶和基因可以完全排除或至少降低活性。 在优选的实施方案中，使用SEQ ID NO：1-7。
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2.发明授权Nucleotide sequences of coryneform bacteria coded for proteins participating in L-serine metabolism and method for microbial production of L-serine 失效编码参与L-丝氨酸代谢的蛋白质的棒状细菌的核苷酸序列和用于L-丝氨酸的微生物生产的方法公开(公告)号：US08119374B2
公开(公告)日：2012-02-21
申请号：US12136190
申请日：2008-06-10
摘要： This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.
摘要翻译： 本发明涉及编码与L-丝氨酸脱水酶活性降低和关闭的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 本发明还涉及用于生产L-丝氨酸的方法中使用的微生物。
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3.发明授权Nucleotide sequences coding for proteins involved in the biosynthesis of L-serine, an improved method for the microbial production of L-serine and a genetically modified microorganism suitable therefor 失效编码参与L-丝氨酸生物合成的蛋白质的核苷酸序列，用于L-丝氨酸微生物生产的改进方法和适用于其的转基因微生物公开(公告)号：US07083952B2
公开(公告)日：2006-08-01
申请号：US10224574
申请日：2002-08-21
CPC分类号： C12P13/06 , C12N9/1096 , C12N9/16
摘要： The present invention relates to nucleotide sequences of coryneform bacteria, coding for proteins involved in the bio-synthesis of L-serine and to methods for the isolation thereof. The invention further relates to an improved method for the production of L-serine. In addition, the present invention relates to the use of L-serine in the food, animal feed and/or pharmaceutical industries or in human medicine.
摘要翻译： 本发明涉及编码参与L-丝氨酸的生物合成的蛋白质的棒状细菌的核苷酸序列及其分离方法。 本发明还涉及一种生产L-丝氨酸的改进方法。 此外，本发明涉及L-丝氨酸在食品，动物饲料和/或制药工业中或在人类药物中的用途。
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4.发明授权Nucleotide sequences that encode deregulated phosphoglycerate dehydrogenases of coryneform bacteria and method for producing L-serine 失效编码棒状杆菌细菌脱磷酸甘油酸脱氢酶的核苷酸序列及其生产L-丝氨酸的方法公开(公告)号：US07732176B2
公开(公告)日：2010-06-08
申请号：US10520999
申请日：2003-07-08
申请人： Lothar Eggeling , Petra Peters-Wendisch , Roman Netzer , Hermann Sahm , Robert Faurie , Birgit Klassen
发明人： Lothar Eggeling , Petra Peters-Wendisch , Roman Netzer , Hermann Sahm , Robert Faurie , Birgit Klassen
CPC分类号： C12N9/0006 , C12P13/06 , C12Y101/01095
摘要： The invention relates to nucleotide sequences of coryneform bacteria that encode proteins that are involved in the biosynthesis of L-serine and to a method for producing L-serine. According to the invention, at least 79 amino acids at the C terminus of the wild-type serA sequence are deleted, thereby producing a 3-phosphoglycerate dehydrogenase having a reduced feedback inhibition by L-serine vis-à-vis the wild-type sequence.
摘要翻译： 本发明涉及编码参与L-丝氨酸生物合成的蛋白质的棒状细菌的核苷酸序列和L-丝氨酸的生产方法。 根据本发明，野生型serA序列的C末端至少有79个氨基酸被缺失，从而产生了与L-丝氨酸相对于野生型序列的反馈抑制减少的3-磷酸甘油酸脱氢酶 。
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5.发明申请Nucleotide sequences that encode deregulated phosphoglycerate dehydrogenases of coryneform bacteria and method for producing l-serine 失效编码棒状杆菌细菌脱磷酸甘油酸脱氢酶的核苷酸序列和产生l-丝氨酸的方法公开(公告)号：US20060106207A1
公开(公告)日：2006-05-18
申请号：US10520999
申请日：2003-07-08
申请人： Lothar Eggeling , Petra Peters-Wendisch , Roman Netzer , Hermann Sahm , Robert Faurie , Birgit Klassen
发明人： Lothar Eggeling , Petra Peters-Wendisch , Roman Netzer , Hermann Sahm , Robert Faurie , Birgit Klassen
IPC分类号： C07H21/04
CPC分类号： C12N9/0006 , C12P13/06 , C12Y101/01095
摘要： The invention relates to nucleotide sequences of coryneform bacteria that encode proteins that are involved in the biosynthesis of L-serine and to a method for producing L-serine. According to the invention, at least 79 amino acids at the C terminus of the wild-type serA sequence are deleted, thereby producing a 3-phosphoglycerate dehydrogenase having a reduced feedback inhibition by L-serine vis-à-vis the wild-type sequence.
摘要翻译： 本发明涉及编码参与L-丝氨酸生物合成的蛋白质的棒状细菌的核苷酸序列和L-丝氨酸的生产方法。 根据本发明，野生型serA序列的C末端至少有79个氨基酸被缺失，从而产生了与L-丝氨酸相对于野生型序列的反馈抑制减少的3-磷酸甘油酸脱氢酶 。
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6.发明申请NUCLEOTIDE SEQUENCES OF CORYNEFORM BACTERIA CODED FOR PROTEINS PARTICIPATING IN L-SERINE METABOLISM AND METHOD FOR MICROBIAL PRODUCTION OF L-SERINE 失效编码用于蛋白质参与L-丝氨酸代谢的CORYNEFORM细菌的核苷酸序列和用于微生物生产L-丝氨酸的方法公开(公告)号：US20090061482A1
公开(公告)日：2009-03-05
申请号：US12136190
申请日：2008-06-10
摘要： This invention relates to the nucleotide sequence of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase activity. The invention also relates to microorganisms used in methods for producing L-serine.
摘要翻译： 本发明涉及编码与L-丝氨酸脱水酶活性降低和关闭的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 本发明还涉及用于生产L-丝氨酸的方法中使用的微生物。
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7.发明申请Nucleotide sequences of coryneform bacteria coding for proteins involved in l-serine metabolism and method for producing l-serine 审中-公开编码参与l-丝氨酸代谢的蛋白质的棒状细菌的核苷酸序列和用于产生l-丝氨酸的方法公开(公告)号：US20060204963A1
公开(公告)日：2006-09-14
申请号：US10549262
申请日：2004-02-12
摘要： The invention relates to the nucleotide sequences of coryneform bacteria coding for proteins which are involved in L-serine metabolism with reduced and switched off L-serine dehydratase. Said invention also relates to micro-organisms and to methods for producing L-serine.
摘要翻译： 本发明涉及编码与减少和关闭L-丝氨酸脱水酶的L-丝氨酸代谢相关的蛋白质的棒状细菌的核苷酸序列。 所述发明还涉及微生物和生产L-丝氨酸的方法。
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8.发明申请Method for microbial production of amino acids of the spartate and/or glutamate family and agents which can be used in said method 审中-公开用于微生物生产燕麦和/或谷氨酸家族的氨基酸的方法和可用于所述方法的试剂公开(公告)号：US20070202571A1
公开(公告)日：2007-08-30
申请号：US11732869
申请日：2007-04-05
IPC分类号： C12P21/00
摘要： A method is disclosed for microbially producing L-amino acids of aspartate and/or glutamate families which comprises the step of: culturing a strain of Escherichia coli that produces said L-amino acids in a medium suitable for the production of an L-amino acid, whereby said Escherichia coli strain is transformed with a gene construct comprising a polynucleotide encoding an enzyme with pyruvate carboxylase activity.
摘要翻译： 公开了一种用于微生物天冬氨酸和/或谷氨酸家族的L-氨基酸的方法，其包括以下步骤：在适于生产L-氨基酸的培养基中培养产生所述L-氨基酸的大肠杆菌菌株 ，其中所述大肠杆菌菌株用包含编码具有丙酮酸羧化酶活性的酶的多核苷酸的基因构建体转化。
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9.发明授权公开(公告)号：US07267967B1
公开(公告)日：2007-09-11
申请号：US09529043
申请日：1998-09-30
摘要： A pyruvate carboxylase gene having SEQ ID NO:1 as well as an amino acid sequence having SEQ ID NO:2 expressed by the pyruvate carboxylate gene are disclosed. Also disclosed is a method for microbial production of amino acids of the aspartate and/or glutamate family in which the pyruvate carboxylase activity is increased by genetically changing the pyruvate carboxylase gene having SEQ ID NO:1 through expression of a microorganism which produces the corresponding amino acid having SEQ ID NO:2.
摘要翻译： 公开了具有SEQ ID NO：1的丙酮酸羧化酶基因以及由丙酮酸羧酸酯基因表达的具有SEQ ID NO：2的氨基酸序列。 还公开了一种微生物生产天冬氨酸和/或谷氨酸家族的氨基酸的方法，其中丙酮酸羧化酶活性通过遗传改变具有SEQ ID NO：1的丙酮酸羧化酶基因而增加，通过表达产生相应氨基的微生物 具有SEQ ID NO：2的酸。
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10.发明申请L-LYSINE-PRODUCING CORYNEBACTERIA AND PROCESS FOR THE PREPARATION OF L-LYSINE 有权生产L-赖氨酸的L-赖氨酸和L-赖氨酸的制备方法公开(公告)号：US20100261233A1
公开(公告)日：2010-10-14
申请号：US12249092
申请日：2008-10-10
申请人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
发明人： Caroline Kreutzer , Stephan Hans , Mechthild Rieping , Bettina Mockel , Walter Pfefferle , Lothar Eggeling , Hermann Sahm , Miroslav Patek
IPC分类号： C12P13/08
摘要： L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.
摘要翻译： 具有增强的lysE基因（赖氨酸出口载体基因）的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因）的lys附加基因，lysC基因（天冬氨酸激酶基因），dapB基因 （二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）增强，特别是过表达，以及制备L-赖氨酸的方法。
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