Optical architectures for microvolume laser-scanning cytometers
    4.
    发明授权
    Optical architectures for microvolume laser-scanning cytometers 失效
    用于微体积激光扫描细胞仪的光学架构

    公开(公告)号:US06603537B1

    公开(公告)日:2003-08-05

    申请号:US09378259

    申请日:1999-08-20

    IPC分类号: G01J330

    摘要: Methods and instrumentation for performing charge coupled device (CCD)-based confocal spectroscopy with a laser spot array are provided. The methods and instruments of the invention are useful in any spectroscopic application, including, but not limited to, microscopy and microvolume laser scanning cytometry (MLSC).

    摘要翻译: 提供了一种用激光点阵进行基于电荷耦合器件(CCD)的共聚焦光谱法的方法和仪器。 本发明的方法和仪器在任何光谱应用中是有用的,包括但不限于显微镜和微体积激光扫描细胞计数(MLSC)。

    Optical architectures for microvolume laser-scanning cytometers
    8.
    发明授权
    Optical architectures for microvolume laser-scanning cytometers 失效
    用于微体积激光扫描细胞仪的光学架构

    公开(公告)号:US06800860B2

    公开(公告)日:2004-10-05

    申请号:US10635917

    申请日:2003-08-05

    IPC分类号: G01N2164

    摘要: Methods and instrumentation for performing charge coupled device (CCD)-based confocal spectroscopy with a laser spot array are provided. The methods and instruments of the invention are useful in any spectroscopic application, including, but not limited to, microscopy and microvolume laser scanning cytometry (MLSC).

    摘要翻译: 提供了一种用激光点阵进行基于电荷耦合器件(CCD)的共聚焦光谱法的方法和仪器。 本发明的方法和仪器在任何光谱应用中是有用的,包括但不限于显微镜和微体积激光扫描细胞计数(MLSC)。

    Mass spectrometic quantification of chemical mixture components
    9.
    发明申请
    Mass spectrometic quantification of chemical mixture components 有权
    化学混合物组分的质谱定量

    公开(公告)号:US20050116159A1

    公开(公告)日:2005-06-02

    申请号:US11023234

    申请日:2004-12-27

    IPC分类号: H01J49/04 H01J49/00

    CPC分类号: H01J49/0036

    摘要: Relative quantitative information about components of chemical or biological samples can be obtained from mass spectra by normalizing the spectra to yield peak intensity values that accurately reflect concentrations of the responsible species. A normalization factor is computed from peak intensities of those inherent components whose concentration remains constant across a series of samples. Relative concentrations of a component occurring in different samples can be estimated from the normalized peak intensities. Unlike conventional methods, internal standards or additional reagents are not required. The methods are particularly useful for differential phenotyping in proteomics and metabolomics research, in which molecules varying in concentration across samples are identified. These identified species may serve as biological markers for disease or response to therapy.

    摘要翻译: 通过对光谱进行归一化,可以从质谱中获得关于化学或生物样品成分的相对定量信息,以产生准确反映负责物种浓度的峰值强度值。 归一化因子是由浓度在一系列样品中保持不变的那些固有成分的峰值强度计算出来的。 发生在不同样品中的组分的相对浓度可以从标准化峰强度估计。 与传统方法不同,不需要内标或附加试剂。 该方法对于蛋白质组学和代谢组学研究中的差异表型特别有用,其中鉴定了样品浓度变化的分子。 这些鉴定的物种可以作为疾病或治疗反应的生物标志物。