公开(公告)号：US20240084401A1

公开(公告)日：2024-03-14

申请号：US17938640

申请日：2022-10-06

Applicant: Mammoth Biosciences, Inc.

Inventor： Matthew VEROSLOFF ,  Clare FASCHING ,  Carley Gelenter HENDRIKS ,  Xin MIAO ,  James Paul BROUGHTON ,  Lucas Benjamin HARRINGTON ,  Janice Sha CHEN

IPC: C12Q1/689 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6844

CPC classification number: C12Q1/689 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12N2310/20

Abstract: Provided herein, in certain embodiments, are various methods, reagents, and devices for detection of multiple target nucleic acids in a sample, or multiple segments of a target nucleic acid in a sample, using a programmable nuclease. In certain embodiments, the present disclosure provides compositions of pools of guide nucleic acids, programmable nucleases, and detector nucleic acids and methods of using said compositions for detection of different segments of one target nucleic acid or different target nucleic acids in a sample.

公开(公告)号：US20240384332A1

公开(公告)日：2024-11-21

申请号：US18393023

申请日：2023-12-21

Applicant: Mammoth Biosciences, Inc.

Inventor： Jesus CHING ,  James Paul BROUGHTON ,  Janice Sha CHEN ,  Clare Louise FASCHING ,  Phillip You Fai LEE ,  Brian J. EASTMAN ,  Devin SPRATT ,  Sarah Jane SHAPIRO ,  Sonal JAIN ,  Matthew VEROSLOFF ,  Jasmeet SINGH ,  Elizabeth M. HAWKINS ,  Vaishnavi NAGESH ,  Lior KREINDLER ,  Sophia HUBBELL ,  Carley Gelenter HENDRIKS ,  Emma G. STANFIELD ,  Amanda Claire BROWN

IPC: C12Q1/6844 ,  B01L3/00 ,  C12Q1/34

Abstract: Various compositions and diagnostic devices for the detection of nucleic acids are provided. Also provided are systems comprising the same, and methods of using the same. In some embodiments, compositions and devices comprise or are configured to be used in combination with one or more of a guide nucleic acid, a programmable nuclease, and reporters. In some embodiments, presence of at least one sequence of interest is determined by detecting a signal produced upon cleavage of the reporters.

公开(公告)号：US20240401158A1

公开(公告)日：2024-12-05

申请号：US18431664

申请日：2024-02-02

Applicant: Mammoth Biosciences, Inc.

Inventor： Clare Louise FASCHING ,  James Paul BROUGHTON ,  Jesus CHING ,  Janice Sha CHEN ,  Carley Gelenter HENDRIKS ,  Bridget Ann Paine MCKAY

IPC: C12Q1/70 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6844

Abstract: Compositions and methods for improved SNP discrimination are provided. An exemplary composition comprises a programmable nuclease and a non-naturally occurring guide nucleic acid that hybridizes to a target nucleic acid, or segment thereof, comprising at least one single-nucleotide polymorphism (SNP). Methods may comprise a) contacting the sample to: i) a detector nucleic acid and ii) the composition; and b) assaying for a signal indicative of cleavage of the detector nucleic acid by the first programmable nuclease. In an exemplary embodiment, the target nucleic acid is a coronavirus target nucleic acid.

公开(公告)号：US20240209421A1

公开(公告)日：2024-06-27

申请号：US18334320

申请日：2023-06-13

Applicant: Mammoth Biosciences, Inc.

Inventor： Janice Sha CHEN ,  Carley Gelenter HENDRIKS ,  Clare Louise FASCHING ,  Xin MIAO ,  Nazmiye Emel ALPAY ,  James Paul BROUGHTON ,  Elizabeth M. HAWKINS ,  Matthew S. VEROSLOFF ,  Sophia HUBBELL ,  Sarah Jane SHAPIRO ,  Lior KREINDLER ,  Jesus CHING

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/44 ,  C12Q1/48 ,  C12Q1/6844

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/111 ,  C12Q1/44 ,  C12Q1/485 ,  C12Q1/6844 ,  C12N2310/20 ,  G01N2333/9126 ,  G01N2333/9128 ,  G01N2333/922

Abstract: The present disclosure provides single-buffer systems that enable efficient and rapid amplification and programmable nuclease enzyme mediated reactions. The single-buffer systems and reagent compositions therein may be used for assaying for a nucleic acid sequence from a sample.

公开(公告)号：US20240326048A1

公开(公告)日：2024-10-03

申请号：US18058122

申请日：2022-11-22

Applicant: MAMMOTH BIOSCIENCES, INC.

Inventor： Clare Louise FASCHING ,  Matthew VEROSLOFF ,  Jesus CHING ,  James Paul BROUGHTON ,  Maria-Nefeli TSALOGLOU ,  Phillip You Fai LEE ,  Timothy James PATNO ,  Janice Sha CHEN ,  Sarah Jane SHAPIRO ,  James Raymond CORDOVA ,  Brian J. EASTMAN ,  Donald Allan LOWMILLER ,  Lior KREINDLER ,  Daniel Thomas DRZAL ,  Devin SPRATT ,  Jacob LESINSKI ,  Joshua BAIK ,  Rachel Nouha DERANEY ,  Sonal JAIN ,  Sophia HUBBELL ,  Carley Gelenter HENDRIKS

IPC: B01L3/00 ,  B01L7/00 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6844

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L7/52 ,  C12N9/22 ,  C12N15/111 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6844 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/1877 ,  B01L2400/0487 ,  C12N2310/20

Abstract: The present disclosure provides various diagnostic devices. Devices can comprising a sample interface configured to receive a sample that may comprise at least one sequence of interest; a channel in fluid communication with the sample interface and a detection chamber, said channel comprising one or more movable mechanisms to separate the sample into a plurality of droplets, wherein said detection chamber is configured to receive and contact the plurality of droplets with at least one programmable nuclease probe disposed on a surface of said detection chamber, and wherein said at least one programmable nuclease probe may comprise a guide nucleic acid complexed with a programmable nuclease; and a plurality of sensors that determine a presence of said at least one sequence of interest by detecting a signal produced upon cleavage of a target nucleic acid region of said at least one sequence of interest by said at least one programmable nuclease probe.

公开(公告)号：US20240191281A1

公开(公告)日：2024-06-13

申请号：US18364359

申请日：2023-08-02

Applicant: Mammoth Biosciences, Inc.

Inventor： Benjamin Julius RAUCH ,  Aaron DELOUGHERY ,  Matan DRORY RETWITZER ,  David PAEZ-ESPINO ,  Caleb TRECAZZI ,  Lucas Benjamin HARRINGTON ,  Janice Sha CHEN ,  James Paul BROUGHTON ,  Clarissa Oriel RHINES ,  William Douglass WRIGHT ,  Matthew VEROSLOFF ,  Carley Gelenter HENDRIKS

IPC: C12Q1/6823 ,  C12N9/22 ,  C12N15/11

CPC classification number: C12Q1/6823 ,  C12N9/22 ,  C12N15/11 ,  C12N2310/20 ,  C12N2310/3517

Abstract: The present disclosure provides compositions and methods of use for a Type VI CRISPR/Cas nuclease. Type VI CRISPR/Cas nucleases are able to bind to a target nucleic acid, thereby activating trans-collateral nuclease activity on nucleic acid reports. Furthermore, the present disclosure provides methods to modify ribonucleic acid sequences using the programmable nucleases disclosed herein.

公开(公告)号：US20240139728A1

公开(公告)日：2024-05-02

申请号：US18055814

申请日：2022-11-15

Applicant: Mammoth Biosciences, Inc.

Inventor： Maria-Nefeli TSALOGLOU ,  Janice Sha CHEN ,  James Paul BROUGHTON ,  Daniel Thomas DRZAL ,  Sarah Jane SHAPIRO ,  Clare Louise FASCHING ,  Carley Gelenter HENDRIKS ,  Jesus CHING ,  Sonal JAIN

IPC: B01L3/00 ,  G01N33/53

CPC classification number: B01L3/5023 ,  G01N33/5308 ,  B01L2300/069 ,  B01L2400/06

Abstract: The application provides devices, kits and methods for a streamlined lateral flow assay for sample preparation, optional amplification, and detection of a target nucleic acid using programmable nuclease reagents.