公开(公告)号：US20240327810A1

公开(公告)日：2024-10-03

申请号：US18501102

申请日：2023-11-03

Applicant: Mammoth Biosciences, Inc.

Inventor： James Paul BROUGHTON ,  Janice Sha Chen ,  Jesus Ching ,  Clare Louise Fasching ,  Lucas Benjamin Harrington ,  Lior Kreindler ,  Bridget Ann Paine Mckay ,  David Paez-Espino ,  Benjamin Julius Rauch ,  Matthew Verosloff ,  William Douglass Wright

IPC: C12N9/22 ,  C12N15/11 ,  C12Q1/6876

CPC classification number: C12N9/22 ,  C12N15/111 ,  C12Q1/6876 ,  C12N2310/20

Abstract: The present disclosure provides compositions of effector proteins. The compositions may comprise engineered guide nucleic acids. Also disclosed are the methods and systems for detecting and modifying target nucleic acids using the same. The compositions may provide nucleic acid modification and/or detection at relatively high temperatures.

公开(公告)号：US20240191280A1

公开(公告)日：2024-06-13

申请号：US18506918

申请日：2023-11-10

Applicant: Mammoth Biosciences, Inc.

Inventor： William Douglass WRIGHT ,  Benjamin Julius RAUCH ,  Aaron DELOUGHERY ,  James Paul BROUGHTON

IPC: C12Q1/6818 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6823 ,  C12Q1/70

CPC classification number: C12Q1/6818 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/6823 ,  C12Q1/701 ,  C12N2310/20 ,  C12N2310/3517 ,  C12N2310/3519 ,  C12N2310/531

Abstract: The present disclosure describes CRISPR/Cas proteins and guide RNA combinations that are capable of modifying nucleic acids at high temperatures. These compositions are especially useful for systems and methods of nucleic acid detection, including diagnostic devices.

公开(公告)号：US20220119788A1

公开(公告)日：2022-04-21

申请号：US17495652

申请日：2021-10-06

Applicant: MAMMOTH BIOSCIENCES, INC.

Inventor： Lucas Benjamin HARRINGTON ,  Janice Sha CHEN ,  James Paul BROUGHTON ,  Pedro Patrick Draper GALARZA ,  Wiputra J. HARTONO ,  Isaac Paterson WITTE ,  Jasmeet SINGH

IPC: C12N9/22 ,  C12N15/113 ,  C12Q1/6886 ,  C12Q1/70

Abstract: Disclosed herein are compositions, kits, and methods related to improved Cas activity. Through compositions and kits disclosed herein and practice of methods disclosed herein, one attains improved Cas activity such as Cas12 activity relative to Cas proteins in the art such as LbCas12. Further described herein are methods to detect target nucleic acid using a programmable nuclease system. Often, the target nucleic acids are present in at low frequency in the sample. Provided herein are methods for enriching these target nucleic acids for detection. Also described herein are methods to insert a PAM sequence into a target sequence of interest for use in a detection comprising a programmable nuclease.

公开(公告)号：US20240359180A1

公开(公告)日：2024-10-31

申请号：US18541650

申请日：2023-12-15

Applicant: Mammoth Biosciences, Inc.

Inventor： Timothy James PATNO ,  Phillip You Fai LEE ,  Benjamin Andrew BLIZARD ,  Xin MIAO ,  Jacob LESINSKI ,  Ryan A. BROWN ,  Daniel Thomas DRZAL ,  Sarah Jane SHAPIRO ,  Joshua BAIK ,  Janice Sha CHEN ,  James Paul BROUGHTON ,  Clare Louise FASCHING ,  Jesus CHING ,  Sonal JAIN ,  Deepika VERMA ,  Matthew VEROSLOFF ,  Devin SPRATT ,  Nicholas John FANTIN

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0663 ,  B01L2300/161 ,  B01L2300/1805 ,  B01L2400/06

Abstract: The present disclosure provides various systems, diagnostic devices, and methods for nucleic acid analysis. The systems, devices, and methods allow for the analysis of nucleic acids, in a sample, via programmable nuclease-based assays. Provided are systems comprising an instrument and a cartridge allowing for point of care use. The systems, devices, and methods described herein can be configured for multiplexed detection of nucleic acids in a single sample.

公开(公告)号：US20240294970A1

公开(公告)日：2024-09-05

申请号：US18334327

申请日：2023-06-13

Applicant: Mammoth Biosciences, Inc.

Inventor： Jesus CHING ,  James Paul BROUGHTON ,  Janice Sha CHEN ,  Sarah Jane SHAPIRO ,  Elizabeth Mae HAWKINS ,  Sonal JAIN ,  Lior KREINDLER ,  Sophia HUBBELL

IPC: C12Q1/6823 ,  C12N15/10 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N21/64

CPC classification number: C12Q1/6823 ,  C12N15/1096 ,  C12Q1/44 ,  C12Q1/6806 ,  C12Q1/6844 ,  G01N21/6428 ,  G01N2021/6432 ,  G01N2333/922

Abstract: Described herein are methods, devices, and compositions for immobilization of biomolecules in diagnostic assays onto a surface. The methods, devices, and compositions may utilize or comprise a plurality of different non-naturally occurring guide nucleic acids. Each of the different non-naturally occurring guide nucleic acids may be immobilized to a surface at a known location identified with the particular non-naturally occurring guide nucleic acid, Alternatively, or in combination, a plurality of programmable nucleases may be immobilized to the surface at each of the known locations. Optionally, the methods, devices, and compositions may utilize or comprise a plurality of reporters immobilized to the surface in proximity to each of the different non-naturally occurring guide nucleic acids and/or programmable nucleases at each of the known locations.

公开(公告)号：US20240084401A1

公开(公告)日：2024-03-14

申请号：US17938640

申请日：2022-10-06

Applicant: Mammoth Biosciences, Inc.

Inventor： Matthew VEROSLOFF ,  Clare FASCHING ,  Carley Gelenter HENDRIKS ,  Xin MIAO ,  James Paul BROUGHTON ,  Lucas Benjamin HARRINGTON ,  Janice Sha CHEN

IPC: C12Q1/689 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6844

CPC classification number: C12Q1/689 ,  C12N9/22 ,  C12Q1/6806 ,  C12Q1/6816 ,  C12Q1/6844 ,  C12N2310/20

Abstract: Provided herein, in certain embodiments, are various methods, reagents, and devices for detection of multiple target nucleic acids in a sample, or multiple segments of a target nucleic acid in a sample, using a programmable nuclease. In certain embodiments, the present disclosure provides compositions of pools of guide nucleic acids, programmable nucleases, and detector nucleic acids and methods of using said compositions for detection of different segments of one target nucleic acid or different target nucleic acids in a sample.

公开(公告)号：US20220072537A1

公开(公告)日：2022-03-10

申请号：US17037592

申请日：2020-09-29

Applicant: MAMMOTH BIOSCIENCES, INC.

Inventor： Janice Sha CHEN ,  Ashley TEHRANCHI ,  Andrew Besancon LANE ,  James Paul BROUGHTON ,  Lucas Benjamin HARRINGTON ,  Maria-Nefeli TSALOGLOU ,  Xin MIAO ,  Clare Louise FASCHING ,  Jasmeet SINGH ,  Pedro Patrick Draper GALARZA

IPC: B01L3/00 ,  C12N9/22 ,  C12Q1/6816 ,  C12N15/113

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

公开(公告)号：US20210102242A1

公开(公告)日：2021-04-08

申请号：US17037594

申请日：2020-09-29

Applicant: MAMMOTH BIOSCIENCES, INC.

Inventor： Janice Sha CHEN ,  Ashley TEHRANCHI ,  Andrew Besancon LANE ,  James Paul BROUGHTON ,  Lucas Benjamin HARRINGTON ,  Maria-Nefeli TSALOGLOU ,  Xin MIAO ,  Clare Louise FASCHING ,  Jasmeet SINGH ,  Pedro Patrick Draper GALARZA

IPC: C12Q1/6827 ,  C12Q1/682

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.

公开(公告)号：US20240384332A1

公开(公告)日：2024-11-21

申请号：US18393023

申请日：2023-12-21

Applicant: Mammoth Biosciences, Inc.

Inventor： Jesus CHING ,  James Paul BROUGHTON ,  Janice Sha CHEN ,  Clare Louise FASCHING ,  Phillip You Fai LEE ,  Brian J. EASTMAN ,  Devin SPRATT ,  Sarah Jane SHAPIRO ,  Sonal JAIN ,  Matthew VEROSLOFF ,  Jasmeet SINGH ,  Elizabeth M. HAWKINS ,  Vaishnavi NAGESH ,  Lior KREINDLER ,  Sophia HUBBELL ,  Carley Gelenter HENDRIKS ,  Emma G. STANFIELD ,  Amanda Claire BROWN

IPC: C12Q1/6844 ,  B01L3/00 ,  C12Q1/34

Abstract: Various compositions and diagnostic devices for the detection of nucleic acids are provided. Also provided are systems comprising the same, and methods of using the same. In some embodiments, compositions and devices comprise or are configured to be used in combination with one or more of a guide nucleic acid, a programmable nuclease, and reporters. In some embodiments, presence of at least one sequence of interest is determined by detecting a signal produced upon cleavage of the reporters.

公开(公告)号：US20220411854A1

公开(公告)日：2022-12-29

申请号：US17687498

申请日：2022-03-04

Applicant: Mammoth Biosciences, Inc.

Inventor： Janice Sha CHEN ,  Ashley TEHRANCHI ,  Andrew Besancon LANE ,  James Paul BROUGHTON ,  Lucas Benjamin HARRINGTON ,  Maria-Nefeli TSALOGLOU ,  Xin MIAO ,  Clare Louise FASCHING ,  Jasmeet SINGH ,  Pedro Patrick Draper GALARZA

IPC: C12Q1/6827 ,  C12Q1/682 ,  B01L3/00 ,  C12N9/22 ,  C12N15/113 ,  C12Q1/6816

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids associated with diseases, cancers, genetic disorders, a genotype, a phenotype, or ancestral origin. The devices, systems, fluidic devices, kits, and methods may comprise reagents of a guide nucleic acid targeting a target nucleic acid, a programmable nuclease, and a single stranded detector nucleic acid with a detection moiety. The target nucleic acid of interest may be indicative of a disease, and the disease may be communicable diseases, or of a cancer or genetic disorder. The target nucleic acid of interest may be indicative of a genotype, a phenotype, or ancestral origin.