公开(公告)号：US20240359180A1

公开(公告)日：2024-10-31

申请号：US18541650

申请日：2023-12-15

Applicant: Mammoth Biosciences, Inc.

Inventor： Timothy James PATNO ,  Phillip You Fai LEE ,  Benjamin Andrew BLIZARD ,  Xin MIAO ,  Jacob LESINSKI ,  Ryan A. BROWN ,  Daniel Thomas DRZAL ,  Sarah Jane SHAPIRO ,  Joshua BAIK ,  Janice Sha CHEN ,  James Paul BROUGHTON ,  Clare Louise FASCHING ,  Jesus CHING ,  Sonal JAIN ,  Deepika VERMA ,  Matthew VEROSLOFF ,  Devin SPRATT ,  Nicholas John FANTIN

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0663 ,  B01L2300/161 ,  B01L2300/1805 ,  B01L2400/06

Abstract: The present disclosure provides various systems, diagnostic devices, and methods for nucleic acid analysis. The systems, devices, and methods allow for the analysis of nucleic acids, in a sample, via programmable nuclease-based assays. Provided are systems comprising an instrument and a cartridge allowing for point of care use. The systems, devices, and methods described herein can be configured for multiplexed detection of nucleic acids in a single sample.

公开(公告)号：US20230332253A1

公开(公告)日：2023-10-19

申请号：US17817889

申请日：2022-08-05

Applicant: Mammoth Biosciences, Inc.

Inventor： Maria-Nefeli TSALOGLOU ,  Clare FASCHING ,  Jesus CHING ,  Janice Sha CHEN ,  James Paul BROUGHTON ,  Daniel Thomas DRZAL ,  Sarah Jane SHAPIRO

IPC: C12Q1/70 ,  C12Q1/6853 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/701 ,  C12Q1/6853 ,  B01L3/502715 ,  B01L7/52 ,  C12Q2600/16 ,  C12Q2600/156 ,  B01L2300/0636 ,  B01L2300/087 ,  B01L2200/16 ,  B01L2300/0883

Abstract: Described herein, in certain embodiments, are methods of assaying for a segment of a coronavirus target nucleic acid in a sample. In some embodiments, the target nucleic acid is from a gene of SARS-CoV-2, or a variant thereof. In some embodiments, methods described herein comprise a) contacting the sample to: i) a detector nucleic acid; and ii) a composition comprising a programmable nuclease and a non-naturally occurring guide nucleic acid, and b) assaying for a change in a signal, wherein the change in the signal is produced by cleavage of the detector nucleic acid by the programmable nuclease. Also provided herein are devices for performing the methods of assaying for a segment of a coronavirus target nucleic acid described herein.

公开(公告)号：US20240326048A1

公开(公告)日：2024-10-03

申请号：US18058122

申请日：2022-11-22

Applicant: MAMMOTH BIOSCIENCES, INC.

Inventor： Clare Louise FASCHING ,  Matthew VEROSLOFF ,  Jesus CHING ,  James Paul BROUGHTON ,  Maria-Nefeli TSALOGLOU ,  Phillip You Fai LEE ,  Timothy James PATNO ,  Janice Sha CHEN ,  Sarah Jane SHAPIRO ,  James Raymond CORDOVA ,  Brian J. EASTMAN ,  Donald Allan LOWMILLER ,  Lior KREINDLER ,  Daniel Thomas DRZAL ,  Devin SPRATT ,  Jacob LESINSKI ,  Joshua BAIK ,  Rachel Nouha DERANEY ,  Sonal JAIN ,  Sophia HUBBELL ,  Carley Gelenter HENDRIKS

IPC: B01L3/00 ,  B01L7/00 ,  C12N9/22 ,  C12N15/11 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6844

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L7/52 ,  C12N9/22 ,  C12N15/111 ,  C12Q1/485 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6834 ,  C12Q1/6844 ,  B01L2200/16 ,  B01L2300/0681 ,  B01L2300/1877 ,  B01L2400/0487 ,  C12N2310/20

Abstract: The present disclosure provides various diagnostic devices. Devices can comprising a sample interface configured to receive a sample that may comprise at least one sequence of interest; a channel in fluid communication with the sample interface and a detection chamber, said channel comprising one or more movable mechanisms to separate the sample into a plurality of droplets, wherein said detection chamber is configured to receive and contact the plurality of droplets with at least one programmable nuclease probe disposed on a surface of said detection chamber, and wherein said at least one programmable nuclease probe may comprise a guide nucleic acid complexed with a programmable nuclease; and a plurality of sensors that determine a presence of said at least one sequence of interest by detecting a signal produced upon cleavage of a target nucleic acid region of said at least one sequence of interest by said at least one programmable nuclease probe.

公开(公告)号：US20240139728A1

公开(公告)日：2024-05-02

申请号：US18055814

申请日：2022-11-15

Applicant: Mammoth Biosciences, Inc.

Inventor： Maria-Nefeli TSALOGLOU ,  Janice Sha CHEN ,  James Paul BROUGHTON ,  Daniel Thomas DRZAL ,  Sarah Jane SHAPIRO ,  Clare Louise FASCHING ,  Carley Gelenter HENDRIKS ,  Jesus CHING ,  Sonal JAIN

IPC: B01L3/00 ,  G01N33/53

CPC classification number: B01L3/5023 ,  G01N33/5308 ,  B01L2300/069 ,  B01L2400/06

Abstract: The application provides devices, kits and methods for a streamlined lateral flow assay for sample preparation, optional amplification, and detection of a target nucleic acid using programmable nuclease reagents.

公开(公告)号：US20220325363A1

公开(公告)日：2022-10-13

申请号：US17555236

申请日：2021-12-17

Applicant: Mammoth Biosciences, Inc.

Inventor： James Paul BROUGHTON ,  Jasmeet Singh ,  Clare Louise FASCHING ,  Maria-Nefeli TSALOGLOU ,  Pedro Patrick Draper GALARZA ,  Janice Sha CHEN ,  Xin MIAO ,  Lucas HARRINGTON ,  Daniel Thomas DRZAL ,  Sarah Jane Shapiro

IPC: C12Q1/70 ,  B01L3/00

Abstract: Described herein are devices, systems, fluidic devices, kits, and methods for detection of target nucleic acids.