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    Methods of Refolding Mammalian Glycosyltranferases
    4.
    发明申请
    Methods of Refolding Mammalian Glycosyltranferases 有权
    复制哺乳动物糖基转移酶的方法

    公开(公告)号：US20070269879A1

    公开(公告)日：2007-11-22

    申请号：US10587769

    申请日：2005-02-04

    申请人： Sami Saribas ,  David Hakes ,  Scott Willett ,  Karl Johnson ,  Daniel Bezila ,  Shawn Defrees

    发明人： Sami Saribas ,  David Hakes ,  Scott Willett ,  Karl Johnson ,  Daniel Bezila ,  Shawn Defrees

    IPC分类号： C12P21/06 ,  C12N9/10

    CPC分类号： C12N9/1048

    摘要： The present invention provides methods of refolding mammalian glycosyltransferases that have been produced in bacterial cells, and methods to use such refolded glycosyltransferases, including glycosyltransferase mutants that have enhanced ability to be refolded. The invention also provides methods of refolding more than one glycosyltransferase in a single vessel, methods to use such refolded glycosyltransferases, and reaction mixtures comprising the refolded glycosyltransferases.

    摘要翻译： 本发明提供了在细菌细胞中产生的哺乳动物糖基转移酶重折叠的方法，以及使用这种重折叠的糖基转移酶（包括具有增强的重折叠能力的糖基转移酶突变体）的方法。 本发明还提供了在单个容器中重折叠多于一种糖基转移酶的方法，使用这种重折叠的糖基转移酶的方法和包含重折叠的糖基转移酶的反应混合物。

    Glycopegylated Granulocyte Colony Stimulating Factor
    8.
    发明申请
    Glycopegylated Granulocyte Colony Stimulating Factor 审中-公开
    糖基化粒细胞集落刺激因子

    公开(公告)号：US20070254836A1

    公开(公告)日：2007-11-01

    申请号：US10579620

    申请日：2004-12-03

    申请人： Shawn Defrees ,  Henrik Clausen ,  David Zopf ,  Zhi-Guang Wang ,  Caryn Bowe ,  Marc Schwartz ,  Bingyuan Wu

    发明人： Shawn Defrees ,  Henrik Clausen ,  David Zopf ,  Zhi-Guang Wang ,  Caryn Bowe ,  Marc Schwartz ,  Bingyuan Wu

    IPC分类号： A61K38/22 ,  A61P31/00 ,  A61P37/04 ,  C07K14/75 ,  C12P21/06

    CPC分类号： A61K38/193 ,  A61K47/60 ,  C07K14/535

    摘要： The present invention provides conjugates between Granulocyte Colony Stimulating Factor and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.

    摘要翻译： 本发明提供粒细胞集落刺激因子和PEG部分之间的缀合物。 缀合物经由完整的糖基连接基团连接，该基团介于并共价连接到肽和修饰基团之间。 通过糖基转移酶的作用，缀合物由糖基化和未糖基化的肽形成。 糖基转移酶将修饰的糖部分连接到肽上的氨基酸或糖基残基上。 还提供了包括缀合物的药物制剂。 缀合物的制备方法也在本发明的范围内。

    MUTANT ENDOGLYCOCERAMIDASES WITH ENHANCED SYNTHETIC ACTIVITY
    10.
    发明申请
    MUTANT ENDOGLYCOCERAMIDASES WITH ENHANCED SYNTHETIC ACTIVITY 审中-公开
    具有增强合成活性的突变内毒素

    公开(公告)号：US20140147891A1

    公开(公告)日：2014-05-29

    申请号：US13950259

    申请日：2013-07-24

    申请人： Karl F. Johnson ,  Shawn Defrees ,  Stephen Withers ,  Mark Vaughan

    发明人： Karl F. Johnson ,  Shawn Defrees ,  Stephen Withers ,  Mark Vaughan

    IPC分类号： C12P19/26

    CPC分类号： C12P19/26 ,  C12N9/2402 ,  C12Y302/01123

    摘要： The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme.

    摘要翻译： 本发明涉及其水解活性已经显着降低或消除的新型神经酰胺糖内切酶，使得该酶可用于从单糖或寡糖和神经酰胺合成糖脂。 更具体地，神经酰胺糖内切酶是天然存在的神经酰胺糖内切酶的突变形式，优选地包含酶的活性位点或亲核位点内的突变，更优选地包含活性位点或亲核位点内的Glu残基的取代突变。 还公开了产生突变型神经酰胺糖内切酶的方法和使用该突变酶酶促合成糖脂的方法。